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1. 发明公开

EP2947454A1 METHOD FOR DETECTING SAMPLE GLYCOSYLATION AND TERMINAL MODIFICATION STATES DURING PROTEIN PURIFICATION PROCESS 审中-公开
标题翻译：方法检测样品和终端的糖基化修饰条件期间的蛋白质纯化方法
公开(公告)号：EP2947454A1
公开(公告)日：2015-11-25
申请号：EP13871613.9
申请日：2013-04-24
申请人： Livzon Mabpharm Inc.
发明人： ZHU, Baoguo , PENG, Yucai , YANG, Jiaming
IPC分类号： G01N30/02
CPC分类号： G01N33/6848 , G01N30/06 , G01N30/7233 , G01N33/6854 , G01N2030/027 , G01N2030/065 , G01N2030/067 , G01N2030/8831 , G01N2440/38 , G01N2560/00 , Y10T436/24
摘要： The present invention provides a method for determining glycosylation and terminal modifications of immunoglobulin during immunoglobulin purification process, which can simultaneously and rapidly determine glycosylation, N-terminal pyroglutamination and C-terminal de-lysination of immunoglobulin. The method comprises: 1) separating immunoglobulin by using cation-exchange resin, and collecting different components in according to retention time; 2) denaturing the components of immunoglobulin obtained in step 1) with a denaturant, followed by reducing them with a reducing agent, to separate the light chain and heavy chain; 3) separating the light chain and heavy chain of immunoglobulin of step 2) by using reverse phase ultrahigh pressure liquid chromatography; 4) measuring the molecular weights of the light chain and heavy chain obtained in step 3) with mass spectrum; 5) analyzing the chromatographic data obtained in step 3) and the mass spectrometric data obtained in step 4) to determine glycosylation and terminal modifications of said immunoglobulin.

2. 发明授权

EP2380017B1 PROCEDE DE DETERMINATION DE LA TENEUR D'UNE PLURALITE DE COMPOSES CONTENUS DANS UN FLUIDE DE FORAGE 有权
标题翻译：用于确定多个链路的浓度的钻井液
公开(公告)号：EP2380017B1
公开(公告)日：2013-04-03
申请号：EP10706289.5
申请日：2010-01-08
申请人： Geoservices Equipements
发明人： LESSI, Jacques
IPC分类号： G01N33/28 , E21B49/08
CPC分类号： E21B21/067 , E21B49/005 , G01N2030/065

3. 发明授权

EP1521631B1 VERFAHREN ZUM MISCHUNGSFREIEN ÜBERTRAGEN VON HETEROGENEN FLÜSSIGKEITEN IN MIKROKANÄLEN 有权转让
标题翻译：方法混合自由TRANSFERRING非均质液体在微流道
公开(公告)号：EP1521631B1
公开(公告)日：2011-07-27
申请号：EP03732468.8
申请日：2003-05-26
申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
发明人： WAGLER, Patrick , MCCASKILL, John, Simpson , FOSTER, Tobias
IPC分类号： B01J19/00
CPC分类号： B01L3/502784 , B01F13/0059 , B01F13/0071 , B01J19/0093 , B01J2219/00837 , B01J2219/0086 , B01J2219/00891 , B01J2219/00916 , B01L3/502723 , B01L3/502753 , B01L2200/0605 , B01L2200/0673 , G01N30/84 , G01N35/08 , G01N2001/4061 , G01N2030/065 , Y10T137/0391 , Y10T137/218 , Y10T436/2575
摘要： The invention relates to a method for transferring heterogeneous liquids in microchannels without the occurrence of mixing, according to which a heterogeneous liquid flow (14) is autonomously portioned, conveyed over a distance and is then autonomously recombined. During portioning, individual volumes of a second liquid (16), which can be completely but not necessarily homogeneous, are introduced between successive partial volumes of the heterogeneous liquid flow (14). Without these measures, partial volumes of the heterogeneous liquid (14) that are in contact with one another would mix together. Therefore, by separating the individual partial volumes, the invention now enables these partial volumes, thus the heterogeneous liquid (14), to be conveyed over long distances without the occurrence of mixing. In order to process the partial volumes of the heterogeneous liquid (14), it is then necessary again to separate the liquid flow (12) from alternately successive partial volumes of both liquids (14, 16) so that two flows are formed again of which one flow is the same as the heterogeneous starting liquid and the other is the same as the second liquid. An overall solution for the conveyance of a heterogeneous liquid without the occurrence of mixing and without the use of expensive fractionators is made possible by virtue of the fact that both processes can autonomously proceed by a self-organization of the phases.

4. 发明授权

EP1131627B1 VORRICHTUNG UND VERFAHREN ZUR PARALLELEN FLÜSSIGCHROMATOGRAPHISCHEN TRENNUNG VON SUBSTANZEN 有权
标题翻译： DEVICE AND METHOD FOR PARALLEL液体色谱分离物质
公开(公告)号：EP1131627B1
公开(公告)日：2006-04-19
申请号：EP99958185.3
申请日：1999-11-22
申请人： SEPIAtec GmbH
发明人： MÜLLER-KUHRT, Lutz , GOD, Ralf; , GUMM, Holger , BINKELE, Jörg
IPC分类号： G01N30/46 , B01D15/08
CPC分类号： G01N30/466 , B01D15/14 , B01D15/1885 , G01N30/02 , G01N30/32 , G01N2030/062 , G01N2030/065 , G01N2030/324 , B01D15/40
摘要： The invention relates to a device and a method for separating substances by liquid chromatography. The aim of the invention is to provide a device and a method by which means substances can be separated by liquid chromatography under pressure and which enable parallel separation and detection of at least several samples. The device should have a compact, economical construction. To this end, the inventive device for separating substances by liquid chromatography under pressure is characterised in that at least several liquid chromatography separating lines (17) are supplied by a single delivery unit (one or two pumps), said separating lines being arranged so that they run parallel, and in that said separating lines are combined with a sample-loading system (5) and an injection system (18) in the sample introduction area and with a multi-channel detector (13), connected to an evaluation and control unit (16), in the detection area.

5. 发明公开

EP0214237A1 METHOD AND APPARATUS FOR ANALYZING GASES DISSOLVED IN A LIQUID SAMPLE 有权
标题翻译：方法和装置，用于分析溶解气体的液体样品。
公开(公告)号：EP0214237A1
公开(公告)日：1987-03-18
申请号：EP86901676.0
申请日：1986-02-25
申请人： GENERAL ELECTRIC COMPANY
发明人： FISHER, David, John
IPC分类号： G01N1 , G01N30
CPC分类号： G01N30/88 , G01N30/06 , G01N30/56 , G01N2030/025 , G01N2030/065 , G01N2030/8804 , G01N2030/8854 , G01N2030/8886
摘要： Un appareil chromatographique automatique à gaz est utilisé pour analyser de façon rapide et fiable différents gaz dissous dans l'huile isolante d'un transformateur de puissance rempli d'huile. Un échantillon d'huile isolante est introduit dans une boucle d'échantillonnage (24) par une soupape d'échantillonage (22). Il est ensuite propulsé par un gaz porteur inerte dans une chambre de dégazage (26) composée d'un tuyau vertical (70) rempli d'éléments sphériques de remplissage (74). En montant dans ce tuyau (70), le gaz porteur extrait les différents gaz de la solution pour les transporter par écoulement à travers le tampon jusqu'à un chromatographe à gaz où les gaz extraits sont séparés et analysés individuellement.

6. 发明公开

EP3242133A1 COMPOSITION AND SYSTEM FOR SEPARATING AND DETECTING ALPHA-FETOPROTEIN VARIANT AND USE THEREOF 有权
标题翻译：用于分离和检测α-FETPROTEIN变体的组合物和系统及其用途
公开(公告)号：EP3242133A1
公开(公告)日：2017-11-08
申请号：EP15875252.7
申请日：2015-12-30
申请人： Beijing Hotgen Biotech Co., Ltd.
发明人： LUO, Haifeng , LI, Boan , LIN, Changqing
IPC分类号： G01N33/68 , G01N33/543
CPC分类号： C07K14/4715 , A61K38/1709 , G01N33/54326 , G01N33/68 , G01N2030/065
摘要： Disclosed are a composition and system for separating and detecting an alpha-fetoprotein variant, comprising a separation reagent and a detection reagent; a system for separating and detecting an alpha-fetoprotein variant and a use thereof; and a kit for separating and detecting the alpha-fetoprotein variant. By means of the composition and system for separating and detecting the alpha-fetoprotein variant, and the use thereof, primary liver cancer can be indicated early on, the sensitivity is high, and the method is rapid, simple and automated.
摘要翻译：公开了用于分离和检测甲胎蛋白变体的组合物和系统，其包含分离试剂和检测试剂; 用于分离和检测甲胎蛋白变体的系统及其用途; 和用于分离和检测甲胎蛋白变体的试剂盒。通过用于分离和检测甲胎蛋白变体的组合物和系统及其用途，可以在早期指示原发性肝癌，灵敏度高，并且该方法快速，简单和自动化。

7. 发明公开

EP2947454A4 METHOD FOR DETECTING SAMPLE GLYCOSYLATION AND TERMINAL MODIFICATION STATES DURING PROTEIN PURIFICATION PROCESS 审中-公开
标题翻译：方法检测样品和终端的糖基化修饰条件期间的蛋白质纯化方法
公开(公告)号：EP2947454A4
公开(公告)日：2016-08-03
申请号：EP13871613
申请日：2013-04-24
申请人： LIVZON MABPHARM INC
发明人： ZHU BAOGUO , PENG YUCAI , YANG JIAMING
IPC分类号： G01N30/02 , G01N33/68
CPC分类号： G01N33/6848 , G01N30/06 , G01N30/7233 , G01N33/6854 , G01N2030/027 , G01N2030/065 , G01N2030/067 , G01N2030/8831 , G01N2440/38 , G01N2560/00 , Y10T436/24
摘要： The present invention provides a method for determining glycosylation and terminal modifications of immunoglobulin during immunoglobulin purification process, which can simultaneously and rapidly determine glycosylation, N-terminal pyroglutamination and C-terminal de-lysination of immunoglobulin. The method comprises: 1) separating immunoglobulin by using cation-exchange resin, and collecting different components in according to retention time; 2) denaturing the components of immunoglobulin obtained in step 1) with a denaturant, followed by reducing them with a reducing agent, to separate the light chain and heavy chain; 3) separating the light chain and heavy chain of immunoglobulin of step 2) by using reverse phase ultrahigh pressure liquid chromatography; 4) measuring the molecular weights of the light chain and heavy chain obtained in step 3) with mass spectrum; 5) analyzing the chromatographic data obtained in step 3) and the mass spectrometric data obtained in step 4) to determine glycosylation and terminal modifications of said immunoglobulin.
摘要翻译：本发明提供了用于确定性采矿糖基化和期间的免疫球蛋白纯化过程免疫球蛋白的末端修饰的方法，该免疫球蛋白可以同时和快速确定性矿糖基化，N-末端和C-末端pyroglutamination脱lysination。该方法包括：1）通过使用阳离子交换树脂，并收集在雅丁保留时间不同组分中分离免疫球蛋白; 2）免疫球蛋白变性的步骤1中获得）与变性剂，随后通过降低它们与还原剂，对轻链和重链的分离部件; 3）通过使用反相超高压液相色谱分离步骤2）的免疫球蛋白的轻链和重链; 4）测量在轻链和与质谱步骤3）中得到的重链的分子量; 5）分析）在步骤3中获得的色谱数据和质谱数据在步骤4中获得的），以确定的糖基化矿和所述免疫球蛋白的末端修饰。

8. 发明公开

EP2876438A4 HEAD SPACE SAMPLE INTRODUCTION DEVICE AND GAS CHROMATOGRAPH INCLUDING SAME 有权
标题翻译： HEADSPACE样品介绍和气相色谱
公开(公告)号：EP2876438A4
公开(公告)日：2016-03-23
申请号：EP12884084
申请日：2012-09-05
申请人： SHIMADZU CORP
发明人： AONO AKIRA
IPC分类号： G01N30/04 , G01N30/00 , G01N30/06 , G01N30/20 , G01N30/24 , G01N30/32
CPC分类号： G01N30/24 , G01N30/32 , G01N2030/009 , G01N2030/065 , G01N2030/202 , G01N2030/207
摘要： A pressurization passage that divides a constant pressure from a pressure source with passage resistances so as to have a predetermined constant pressure is disposed on a downstream side of a sample loop in order to maintain a back pressure of the sample loop constant when a sample gas is collected to the sample loop.

9. 发明公开

EP1666114A4 METHOD AND DEVICE FOR SUBSTANCE SEPARATION USING COMPATIBLE MULTIPHASE SOLVENT SYSTEM 审中-公开
标题翻译：方法和装置物的分离使用兼容的多相溶剂体系
公开(公告)号：EP1666114A4
公开(公告)日：2008-01-09
申请号：EP04771394
申请日：2004-08-03
申请人： UNIV TOKYO AGRICULTURE & TECHNOLOGY TLO CO LTD
发明人： CHIBA KAZUHIRO
IPC分类号： B01D11/04 , G01N30/30 , G01N30/42
CPC分类号： G01N30/30 , G01N2030/3015 , G01N2030/065
摘要： [PROBLEMS] To provide a method and a device for separating object substances from a mixture without requiring an ultrasonic generator and a centrifugal separator. [MEANS FOR SOLVING PROBLEMS] In this method of separating the object substances from the mixture by using a first solvent and a second solvent, the phases of the first solvent and the second solvent are reversibly changed from a state of being separated into two phases to a state of being mutually dissolved into one phase by changing their temperatures. The upstream portion and the center portion of a column including the first solvent are held in a temperature range in which the solvents are mutually dissolved into one phase, and the downstream portion of the column is held in a temperature range in which the solvents are separated into two phases. The second solvent in which the mixture is dissolved is charged from the upstream portion of the column into the column, and the separated second solvent phase is taken out from the downstream portion of the column.

10. 发明公开

EP1666114A1 METHOD AND DEVICE FOR SUBSTANCE SEPARATION USING COMPATIBLE MULTIPHASE SOLVENT SYSTEM 审中-公开
标题翻译：方法和装置物的分离使用兼容的多相溶剂体系
公开(公告)号：EP1666114A1
公开(公告)日：2006-06-07
申请号：EP04771394.6
申请日：2004-08-03
申请人： Tokyo University of Agriculture and Technology Tlo Co., Ltd.
发明人： CHIBA, K., Agriculture Dept., Tokyo University of
IPC分类号： B01D11/04
CPC分类号： G01N30/30 , G01N2030/3015 , G01N2030/065
摘要： A method for separating a target substance from a mixture using a first solvent and a second solvent wherein the first solvent and the second solvent undergo a reversible phase change from a biphasically separated state into a mutually dissolved monophasic state by means of changing the temperature, wherein an upstream region and an intermediate region of a column containing the first solvent are kept within a range of the temperature allowing for a monophasic mutual dissolution and a downstream region of the column is kept within a range of the temperature allowing for a biphasic separation, wherein the second solvent containing the mixture dissolved therein is loaded onto the column from the upstream of the column and thereafter the second solvent phase separated in the downstream region of the column is taken out whereby separating the target substance.
摘要翻译：一种用于使用第一溶剂和第二溶剂worin所述第一溶剂和所述第二溶剂，借助于经历从双相分离状态成相互溶解的单相状态的可逆相变从混合物中分离目标物质的方法改变温度，worin的在上游区域，并在包含所述第一溶剂被保持的范围内的温度，允许单相互溶解和柱的下游区域内的柱的中间区域被保持在范围的温度允许的两相分离，worin的内含溶解于其中的混合物的第二溶剂被装载到从塔的上游，然后在该塔的下游区域分离的第二溶剂相的柱取出，由此分离目标物质。

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