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    • 1. 发明授权
    • Lysozyme assay
    • 溶菌酶测定
    • US4255517A
    • 1981-03-10
    • US9379
    • 1979-02-02
    • Larry C. Ford
    • Larry C. Ford
    • C12Q1/34
    • C12Q1/34G01N2333/305G01N2333/924Y10S435/81
    • A new and improved assay for the determination of the enzyme lysozyme in biological samples which utilizes a plate assay based upon the bacterium Micrococcus lysodiekticus is described. The assay utilizes a novel composition for the assay medium which results in a reduction in assay incubation time to the point that the assay is complete after one-half hour. This reduction in time is achieved in part by the utilization of organic buffers in the assay medium and in part by the reduction of the ionic strength of the assay medium. by the use of this assay a novel technique for determining the integrity of the amniochorial membrane and maturity of fetal lungs are described.
    • 描述了一种用于测定生物样品中酶溶菌酶的新的和改进的测定法,其利用基于细菌微球菌溶血杆菌的平板测定法。 该测定法利用了用于测定培养基的新型组合物,其导致测定孵育时间的降低,直到测定在半小时后完成。 这个时间的减少部分地通过在测定培养基中利用有机缓冲液,部分地通过降低测定培养基的离子强度来实现。 通过使用该测定法,描述了确定胎膜的完整性和胎儿肺成熟度的新技术。