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1. 发明公开

CN108779442A 多种连接酶的组合物、系统以及方法有权
公开(公告)号：CN108779442A
公开(公告)日：2018-11-09
申请号：CN201780015885.4
申请日：2017-02-07
申请人：瑞尔基因公司
发明人：郑钰 , 洪曼青
IPC分类号： C12N9/00 , C12N15/10 , C07K14/01 , C07H21/04 , C12P19/34
CPC分类号： C12N9/93 , C12Y605/01001 , C12Y605/01002
摘要：本文提供了使用多种连接酶的组合物、系统、以及方法，其中至少一种连接酶是腺苷酸化缺陷型ATP依赖性连接酶或未腺苷酸化的ATP依赖性连接酶(例如存在于无ATP混合物中)。在某些实施方式中，使用多种连接酶来使预腺苷酸化的双链序列与非腺苷酸化的双链序列连接(例如所述腺苷酸化缺陷型ATP依赖性连接酶或未腺苷酸化的ATP依赖性连接酶连接第一链，并且第二连接酶连接第二链)。在其他实施方式中，本文提供了突变型T4连接酶(例如K159S突变体或K159C突变体)。

2. 发明公开

US20240240216A1 MATERIAL AND METHOD FOR PREPARATION OF SMALL, SINGLE-STRANDED DNA CIRCLES 审中-公开
公开(公告)号：US20240240216A1
公开(公告)日：2024-07-18
申请号：US18411891
申请日：2024-01-12
申请人： Purdue Research Foundation
发明人： Chengde Mao , Victoria Elizabeth Paluzzi , Cuizheng Zhang
IPC分类号： C12P19/34 , C12N9/00
CPC分类号： C12P19/34 , C12N9/93 , C12Y605/01002
摘要： A splint single-stranded DNA (ssDNA), which comprises a linear splint sequence and a hairpin sequence on each side of the linear splint sequence; a splint ssDNA-ligating linear ssDNA monomeric complex; a method of producing a ssDNA circle; and a kit comprising the splint ssDNA and instructions for using the splint ssDNA to produce a ssDNA circle.

3. 发明申请

US20180127822A1 MODIFIED NUCLEOTIDES METHODS AND KITS 审中-公开
公开(公告)号：US20180127822A1
公开(公告)日：2018-05-10
申请号：US15792875
申请日：2017-10-25
申请人： Pierce Biotechnology, Inc.
发明人： Christopher L. Etienne , Kay K. Opperman , Barbara J. Kaboord , Scott D. Meier , Jean-Samuel Schultz
IPC分类号： C12Q1/6876 , C12P19/34 , C07H19/10 , C07H19/20
CPC分类号： C12Q1/6876 , C07H19/10 , C07H19/20 , C12P19/34 , C12Q1/6816 , C12Y605/01001 , C12Y605/01002 , C12Y605/01003 , C12Q2525/117 , C12Q2563/131
摘要： Modified nucleotides, and methods to modify nucleotides with a moiety or label, such as biotin, that permits their detection and results in a modified nucleotide, and methods of use of the modified nucleotide in quantitative and qualitative assays.

4. 发明授权

US10626390B2 Multiple ligase compositions, systems, and methods 有权
公开(公告)号：US10626390B2
公开(公告)日：2020-04-21
申请号：US15426543
申请日：2017-02-07
申请人： RGENE, Inc.
发明人： Yu Zheng , Manqing Hong
IPC分类号： C12N9/00
CPC分类号： C12N9/93 , C12Y605/01001 , C12Y605/01002
摘要： Provided herein are compositions, systems, and methods using multiple ligases, wherein at least one of the ligases is an adenylation-deficient ATP-dependent ligase or an un-adenylated ATP-dependent ligase (e.g., present in an ATP free mixture). In certain embodiments, multiple ligases are used to ligate a pre-adenylated double stranded sequence to a non-adenylated double stranded sequence (e.g., the adenylation-deficient ATP-dependent ligase or un-adenylated ATP-dependent ligase ligates the first strand, and a second ligase ligates the second strand). In other embodiments, provided herein are mutant T4 ligases (e.g., K159S mutant or K159C mutant).

5. 发明申请

US20120252691A1 MODIFIED NUCLEOTIDES METHODS AND KITS 有权
标题翻译：改良的核苷酸方法和试剂盒
公开(公告)号：US20120252691A1
公开(公告)日：2012-10-04
申请号：US13482927
申请日：2012-05-29
申请人： Christopher L. Etienne , Kay K. Opperman , Barbara J. Kaboord , Scott Meier , Jean-Samuel Schultz
发明人： Christopher L. Etienne , Kay K. Opperman , Barbara J. Kaboord , Scott Meier , Jean-Samuel Schultz
IPC分类号： C07H19/10 , C12Q1/68 , C40B30/04 , G01N33/566 , C07K17/10 , C07H21/02
CPC分类号： C12Q1/6876 , C07H19/10 , C07H19/20 , C12P19/34 , C12Q1/6816 , C12Y605/01001 , C12Y605/01002 , C12Y605/01003 , C12Q2525/117 , C12Q2563/131
摘要： Modified nucleotides, and methods to modify nucleotides with a moiety or label, such as biotin, that permits their detection and results in a modified nucleotide, and methods of use of the modified nucleotide in quantitative and qualitative assays.
摘要翻译：修饰的核苷酸以及修饰具有部分或标记（例如生物素）的核苷酸的方法，其允许其检测并产生修饰的核苷酸，以及在定量和定性测定中使用经修饰的核苷酸的方法。

6. 发明申请

US20170226498A1 MULTIPLE LIGASE COMPOSITIONS, SYSTEMS, AND METHODS 审中-公开
公开(公告)号：US20170226498A1
公开(公告)日：2017-08-10
申请号：US15426543
申请日：2017-02-07
申请人： RGENE, Inc.
发明人： Yu Zheng , Manqing Hong
IPC分类号： C12N9/00
CPC分类号： C12N9/93 , C12Y605/01001 , C12Y605/01002
摘要： Provided herein are compositions, systems, and methods using multiple ligases, wherein at least one of the ligases is an adenylation-deficient ATP-dependent ligase or an un-adenylated ATP-dependent ligase (e.g., present in an ATP free mixture). In certain embodiments, multiple ligases are used to ligate a pre-adenylated double stranded sequence to a non-adenylated double stranded sequence (e.g., the adenylation-deficient ATP-dependent ligase or un-adenylated ATP-dependent ligase ligates the first strand, and a second ligase ligates the second strand). In other embodiments, provided herein are mutant T4 ligases (e.g., K159S mutant or K159C mutant).

7. 发明申请

US20160153023A1 BIOSENSORS THAT DETECT NAD+ 审中-公开
标题翻译：检测NAD +的生物传感器
公开(公告)号：US20160153023A1
公开(公告)日：2016-06-02
申请号：US14948161
申请日：2015-11-20
申请人： Richard Goodman , Michael Cohen , Lulu Cambronne , Melissa Stewart
发明人： Richard Goodman , Michael Cohen , Lulu Cambronne , Melissa Stewart
IPC分类号： C12Q1/25 , C12N9/00
CPC分类号： C12Q1/25 , C12N9/93 , C12Q1/008 , C12Y605/01002 , G01N2333/9015
摘要： A polypeptide biosensor that detects free NAD+ is disclosed. The polypeptide comprises a first fragment from an NAD+ dependent DNA ligase acetylation domain, a second fragment from the NAD+ dependent DNA ligase acetylation domain, and a fluorescent protein, wherein the fluorescent protein is positioned between the two DNA ligase acetylation domain fragments. Also disclosed are expression vectors comprising the biosensor as well as methods of using the biosensor to detect NAD+.
摘要翻译：公开了检测游离NAD +的多肽生物传感器。多肽包含来自NAD +依赖性DNA连接酶乙酰化结构域的第一片段，来自NAD +依赖性DNA连接酶乙酰化结构域的第二片段和荧光蛋白，其中所述荧光蛋白位于两个DNA连接酶乙酰化结构域片段之间。还公开了包含生物传感器的表达载体以及使用生物传感器检测NAD +的方法。

8. 发明授权

US09828633B1 Modified nucleotides methods and kits 有权
公开(公告)号：US09828633B1
公开(公告)日：2017-11-28
申请号：US14883810
申请日：2015-10-15
申请人： Pierce Biotechnology, Inc.
发明人： Christopher L. Etienne , Kay K. Opperman , Barbara J. Kaboord , Scott D. Meier , Jean-Samuel Schultz
IPC分类号： C07H19/20 , C07H19/10 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C07H19/10 , C07H19/20 , C12P19/34 , C12Q1/6816 , C12Y605/01001 , C12Y605/01002 , C12Y605/01003 , C12Q2525/117 , C12Q2563/131
摘要： Modified nucleotides, and methods to modify nucleotides with a moiety or label, such as biotin, that permits their detection and results in a modified nucleotide, and methods of use of the modified nucleotide in quantitative and qualitative assays.

9. 发明授权

US09206216B2 Modified nucleotides methods and kits 有权
标题翻译：修饰的核苷酸方法和试剂盒
公开(公告)号：US09206216B2
公开(公告)日：2015-12-08
申请号：US13482927
申请日：2012-05-29
申请人： Christopher L. Etienne , Kay K. Opperman , Barbara J. Kaboord , Scott Meier , Jean-Samuel Schultz
发明人： Christopher L. Etienne , Kay K. Opperman , Barbara J. Kaboord , Scott Meier , Jean-Samuel Schultz
IPC分类号： C07H19/10 , C07H19/20 , C12Q1/68
CPC分类号： C12Q1/6876 , C07H19/10 , C07H19/20 , C12P19/34 , C12Q1/6816 , C12Y605/01001 , C12Y605/01002 , C12Y605/01003 , C12Q2525/117 , C12Q2563/131
摘要： Modified nucleotides, and methods to modify nucleotides with a moiety or label, such as biotin, that permits their detection and results in a modified nucleotide, and methods of use of the modified nucleotide in quantitative and qualitative assays.
摘要翻译：修饰的核苷酸以及修饰具有部分或标记（例如生物素）的核苷酸的方法，其允许其检测并产生修饰的核苷酸，以及在定量和定性测定中使用经修饰的核苷酸的方法。

10. 发明申请

US20120156731A1 Improved Methods for Rapid Gene Synthesis 审中-公开
标题翻译：改进的快速基因合成方法
公开(公告)号：US20120156731A1
公开(公告)日：2012-06-21
申请号：US12052156
申请日：2008-03-20
申请人： Yanyi Huang , James Berger , Stephen Quake
发明人： Yanyi Huang , James Berger , Stephen Quake
IPC分类号： C12P19/34 , C07H21/04 , C12M1/00 , C12N9/00
CPC分类号： C12Y605/01001 , C12P19/34 , C12Y605/01002
摘要： Disclosed are methods and materials for assembling long polynucleotides from synthetic oligonucleotides. The use of synthetic oligonucleotides permits non-natural design of sequences. The oligonucleotides used for construction may be relatively short, according to practicalities of nucleotide synthesis. They are assembled using a ligase which is operative over a range of temperatures, i.e., is thermostable. The method and oligonucleotides are designed such that the melting temperature of the strands to be hybridized is set at a number of selected specific temperatures for each group of oligonucleotides to be hybridized and ligated. Hybridization and ligation take place at or near the melting temperature, so that each succeeding ligation is governed by a temperature that will prevent hybridization if any mismatches are present.
摘要翻译：公开了用于从合成寡核苷酸组装长多核苷酸的方法和材料。合成寡核苷酸的使用允许序列的非自然设计。根据核苷酸合成的实用性，用于构建的寡核苷酸可能相对较短。它们使用在一定温度范围内操作的连接酶组装，即是耐热的。设计该方法和寡核苷酸使待杂交的链的解链温度被设定为要混合并连接的每组寡核苷酸的选定的特定温度的数目。杂交和连接在熔融温度或接近熔化温度下进行，因此每次后续连接都由温度控制，如果存在任何错配，将会阻止杂交。

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