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    • 6. 发明授权
    • Established Maruca vitrata cell line
    • 建立了Maruca vitrata细胞系
    • US08088619B2
    • 2012-01-03
    • US11969799
    • 2008-01-04
    • Chung-Hsiung WangChih-Yu WuSong-Tay Lee
    • Chung-Hsiung WangChih-Yu WuSong-Tay Lee
    • C12N5/00C12N5/07
    • C12N5/0601
    • The present invention relates to Maruca vitrata cell lines established from pupal tissues of M. vitrata, including NTU-MV, two subclones of NTU-MV: NTU-MV-1 and NTU-MV-2. The NTU-MV cell line was confirmed to be derived from M. vitrata, and NTU-MV-1, and NTU-MV-2 were confirmed to be derived from NTU-MV by karyotype analysis, Internal transcribed spacer (ITS) analysis and isozyme analysis. The genotypes and characteristics of the abovementioned 3 cell lines are totally different from other insect cell lines so that they are newly established cell lines. In addition, these three MV cell lines are susceptible to Maruca vitrata multiple nucleopolyhedrovirus (MaviMNPV). Therefore the 3 cell lines according to invention can be applied in multiplication of MaviMNPV in vitro to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of baculovirus to produce recombinant proteins.
    • 本发明涉及由薇皮菌的蛹组织构成的马拉维氏玻璃体细胞系,包括NTU-MV,NTU-MV:NTU-MV-1和NTU-MV-2的两个亚克隆。 证实NTU-MV细胞系来源于玻璃体,NTU-MV-1和NTU-MV-2通过核型分析,内转录间隔(ITS)分析和NTN-MV- 同工酶分析。 上述3种细胞系的基因型和特征与其他昆虫细胞系完全不同,因此它们是新建立的细胞系。 此外,这三种MV细胞系对Maruca vitrata多重核苷酸多角体病毒(MaviMNPV)敏感。 因此,根据本发明的3种细胞系可以在MaviMNPV的体外增殖中应用,以在病虫害防治中产生生物杀虫剂。 此外,细胞系也可以用作杆状病毒表达载体的宿主以产生重组蛋白。
    • 7. 发明申请
    • NEWLY ESTABLISHED CELL LINES FROM MARUCA VITRATA
    • 从MARUCA VITRATA新建的细线
    • US20090098649A1
    • 2009-04-16
    • US11969799
    • 2008-01-04
    • Chung-Hsiung WangChih-Yu WuSong-Tay Lee
    • Chung-Hsiung WangChih-Yu WuSong-Tay Lee
    • C12N5/00
    • C12N5/0601
    • The present invention relates to Maruca vitrata cell lines established from pupal tissues of M. vitrata, including NTU-MV, two subclones of NTU-MV: NTU-MV-1 and NTU-MV-2. The NTU-MV cell line was confirmed to be derived from M. vitrata, and NTU-MV-1, and NTU-MV-2 were confirmed to be derived from NTU-MV by karyotype analysis, Internal transcribed spacer (ITS) analysis and isozyme analysis. The genotypes and characteristics of the abovementioned 3 cell lines are totally different from other insect cell lines so that they are newly established cell lines. In addition, these three MV cell lines are susceptible to Maruca vitrata multiple nucleopolyhedrovirus (MaviMNPV). Therefore the 3 cell lines according to invention can be applied in multiplication of MaviMNPV in vitro to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of said baculovirus to produce recombinant proteins.
    • 本发明涉及由薇皮菌的蛹组织构成的马拉维氏玻璃体细胞系,包括NTU-MV,NTU-MV:NTU-MV-1和NTU-MV-2的两个亚克隆。 证实NTU-MV细胞系来源于玻璃体,NTU-MV-1和NTU-MV-2通过核型分析,内转录间隔(ITS)分析和NTN-MV- 同工酶分析。 上述3种细胞系的基因型和特征与其他昆虫细胞系完全不同,因此它们是新建立的细胞系。 此外,这三种MV细胞系对Maruca vitrata多重核苷酸多角体病毒(MaviMNPV)敏感。 因此,根据本发明的3种细胞系可以在MaviMNPV的体外增殖中应用,以在病虫害防治中产生生物杀虫剂。 此外,细胞系也可以用作所述杆状病毒的表达载体的宿主以产生重组蛋白。
    • 9. 发明申请
    • POWERING CIRCUIT OF AC-DC CONVERTER
    • AC-DC转换器的供电电路
    • US20090213629A1
    • 2009-08-27
    • US12098512
    • 2008-04-07
    • Hsing-Fu LIUChin SunChih-Yu Wu
    • Hsing-Fu LIUChin SunChih-Yu Wu
    • H02M7/04
    • H02M3/155H02M7/155H02M7/2176
    • A powering circuit of an AC-DC converter, for converting a high AC input voltage into a low DC output voltage to provide a load voltage in a stable DC bias range, includes a rectifier, a sensing circuit, a control switching circuit, and a voltage regulating capacitor. The rectifier has a primary side coupled to an AC power supply and a secondary side for outputting a DC power supply. The sensing circuit compares the AC input voltage with a preset reference voltage, and turns on a second switch in the control switching circuit when the AC input voltage is lower than the reference voltage, thereby providing a low DC output voltage. The control switching circuit sustains the DC output voltage in a stable DC bias range. Therefore, in addition to reducing the power consumption of the second switch, this circuit structure is simple and can achieve the purpose of circuit integration.
    • AC-DC转换器的供电电路,用于将高交流输入电压转换成低直流输出电压以提供稳定的直流偏置范围内的负载电压,包括整流器,感测电路,控制开关电路和 调压电容器。 整流器具有耦合到AC电源的初级侧和用于输出DC电源的次级侧。 感测电路将交流输入电压与预设参考电压进行比较,当交流输入电压低于参考电压时,接通控制开关电路中的第二开关,从而提供低直流输出电压。 控制开关电路在稳定的直流偏置范围内维持直流输出电压。 因此,除了降低第二开关的功耗外,该电路结构简单,可以达到电路集成的目的。
    • 10. 发明申请
    • ESTABLISHED CELL LINES FROM LYMANTRIA XYLINA
    • 从LYMANTRIA XYLINA建立细胞系
    • US20080299653A1
    • 2008-12-04
    • US11839500
    • 2007-08-15
    • Chung-Hsiung WANGChih-Yu WuShih-Chia Yeh
    • Chung-Hsiung WANGChih-Yu WuShih-Chia Yeh
    • C12N5/06
    • C12N5/0601
    • The present invention relates to Lymantria xylina cell lines established from pupal tissues of L. xylina Swinhoe, including NTU-LY-1, NTU-LY-2, NTU-LY-3, and NTU-LY-4. These four cell lines were confirmed to be newly established cell lines derived from L. xylina by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and isozyme analysis. The genotypes and characteristics of the abovementioned cell lines are totally different from other insect cell lines. In addition, these four L. xylina cell lines are susceptible to insect baculovirus of L. xylina multiple nucleopolyhedrovirus (LyxyMNPV), LdMNPV-like virus, PenuMNPV, as well as microsporidia of Nosema sp. (isolated from Eurema blanda) and N. bombycis and the like. Therefore the invention can be applied in multiplication of the abovementioned insect-pathogenic microorganisms to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of said baculovirus to produce recombinant proteins.
    • 本发明涉及由木霉菌Swinhoe的蛹组织构成的Lymantria xylina细胞系,包括NTU-LY-1,NTU-LY-2,NTU-LY-3和NTU-LY-4。 通过随机扩增多态性DNA聚合酶链反应(RAPD-PCR)和同工酶分析证实这四种细胞系是新建立的来自木霉菌的细胞系。 上述细胞系的基因型和特征与其他昆虫细胞系完全不同。 此外,这四种木霉素细胞系对L.xylina多核苷酸多角体病毒(LyxyMNPV),LdMNPV样病毒,PenuMNPV以及Nosema sp。的微孢子虫的昆虫杆状病毒敏感。 (分离自Eurema blanda)和N. bombycis等。 因此,本发明可以应用于上述昆虫病原微生物的繁殖,以在病虫害防治中产生生物杀虫剂。 此外,细胞系也可以用作所述杆状病毒表达载体的宿主以产生重组蛋白。