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1. 发明授权

US08088619B2 Established Maruca vitrata cell line 有权
标题翻译：建立了Maruca vitrata细胞系
公开(公告)号：US08088619B2
公开(公告)日：2012-01-03
申请号：US11969799
申请日：2008-01-04
申请人： Chung-Hsiung Wang , Chih-Yu Wu , Song-Tay Lee
发明人： Chung-Hsiung Wang , Chih-Yu Wu , Song-Tay Lee
IPC分类号： C12N5/00 , C12N5/07
CPC分类号： C12N5/0601
摘要： The present invention relates to Maruca vitrata cell lines established from pupal tissues of M. vitrata, including NTU-MV, two subclones of NTU-MV: NTU-MV-1 and NTU-MV-2. The NTU-MV cell line was confirmed to be derived from M. vitrata, and NTU-MV-1, and NTU-MV-2 were confirmed to be derived from NTU-MV by karyotype analysis, Internal transcribed spacer (ITS) analysis and isozyme analysis. The genotypes and characteristics of the abovementioned 3 cell lines are totally different from other insect cell lines so that they are newly established cell lines. In addition, these three MV cell lines are susceptible to Maruca vitrata multiple nucleopolyhedrovirus (MaviMNPV). Therefore the 3 cell lines according to invention can be applied in multiplication of MaviMNPV in vitro to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of baculovirus to produce recombinant proteins.
摘要翻译：本发明涉及由薇皮菌的蛹组织构成的马拉维氏玻璃体细胞系，包括NTU-MV，NTU-MV：NTU-MV-1和NTU-MV-2的两个亚克隆。证实NTU-MV细胞系来源于玻璃体，NTU-MV-1和NTU-MV-2通过核型分析，内转录间隔（ITS）分析和NTN-MV- 同工酶分析。上述3种细胞系的基因型和特征与其他昆虫细胞系完全不同，因此它们是新建立的细胞系。此外，这三种MV细胞系对Maruca vitrata多重核苷酸多角体病毒（MaviMNPV）敏感。因此，根据本发明的3种细胞系可以在MaviMNPV的体外增殖中应用，以在病虫害防治中产生生物杀虫剂。此外，细胞系也可以用作杆状病毒表达载体的宿主以产生重组蛋白。

2. 发明申请

US20090098649A1 NEWLY ESTABLISHED CELL LINES FROM MARUCA VITRATA 有权
标题翻译：从MARUCA VITRATA新建的细线
公开(公告)号：US20090098649A1
公开(公告)日：2009-04-16
申请号：US11969799
申请日：2008-01-04
申请人： Chung-Hsiung Wang , Chih-Yu Wu , Song-Tay Lee
发明人： Chung-Hsiung Wang , Chih-Yu Wu , Song-Tay Lee
IPC分类号： C12N5/00
CPC分类号： C12N5/0601
摘要： The present invention relates to Maruca vitrata cell lines established from pupal tissues of M. vitrata, including NTU-MV, two subclones of NTU-MV: NTU-MV-1 and NTU-MV-2. The NTU-MV cell line was confirmed to be derived from M. vitrata, and NTU-MV-1, and NTU-MV-2 were confirmed to be derived from NTU-MV by karyotype analysis, Internal transcribed spacer (ITS) analysis and isozyme analysis. The genotypes and characteristics of the abovementioned 3 cell lines are totally different from other insect cell lines so that they are newly established cell lines. In addition, these three MV cell lines are susceptible to Maruca vitrata multiple nucleopolyhedrovirus (MaviMNPV). Therefore the 3 cell lines according to invention can be applied in multiplication of MaviMNPV in vitro to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of said baculovirus to produce recombinant proteins.
摘要翻译：本发明涉及由薇皮菌的蛹组织构成的马拉维氏玻璃体细胞系，包括NTU-MV，NTU-MV：NTU-MV-1和NTU-MV-2的两个亚克隆。证实NTU-MV细胞系来源于玻璃体，NTU-MV-1和NTU-MV-2通过核型分析，内转录间隔（ITS）分析和NTN-MV- 同工酶分析。上述3种细胞系的基因型和特征与其他昆虫细胞系完全不同，因此它们是新建立的细胞系。此外，这三种MV细胞系对Maruca vitrata多重核苷酸多角体病毒（MaviMNPV）敏感。因此，根据本发明的3种细胞系可以在MaviMNPV的体外增殖中应用，以在病虫害防治中产生生物杀虫剂。此外，细胞系也可以用作所述杆状病毒的表达载体的宿主以产生重组蛋白。

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