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    • 2. 发明授权
    • Method for evaluating toxicity of chemical using alga
    • 使用藻类评价化学毒性的方法
    • US08658392B2
    • 2014-02-25
    • US13508436
    • 2010-10-26
    • Masakazu KatsumataAyano TakeuchiKimiko Kazumura
    • Masakazu KatsumataAyano TakeuchiKimiko Kazumura
    • C12Q1/04
    • G01N21/77G01N21/6408G01N21/6486G01N2021/7786
    • The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value.
    • 本发明提供一种通过使用藻类来评价化学品的毒性的方法,其特征在于,包括:(a)通过加热来解冻冷冻藻类细胞的解冻步骤,并通过向其中加入培养基稀释所得细胞的悬浮液; (b)在解冻步骤(a)中培养获得的藻细胞以使藻类细胞从冷冻和解冻的作用中恢复的回收培养步骤; (c)在回收培养步骤(b)之后收集部分藻类细胞的确认步骤,通过向其中加入培养基稀释该部分藻类细胞,并测定其中的延迟发光的发光量 藻细胞作为初始值数据; (d)在确认步骤(c)之后将藻类细胞与含有测试物质的溶液混合以制备曝光样品并培养曝光样品的曝光步骤; (e)在曝光步骤(d)之后测量曝光样品的延迟发光的发光量作为曝光数据的测量步骤; 以及(f)基于初始值数据和曝光数据计算评价值,并根据评价值来评价被检物质的毒性的评价步骤。
    • 3. 发明申请
    • METHOD FOR EVALUATING TOXICITY OF CHEMICAL USING ALGA
    • 使用ALGA评估化学毒性的方法
    • US20120231491A1
    • 2012-09-13
    • US13508436
    • 2010-10-26
    • Masakazu KatsumataAyano TakeuchiKimiko Kazumura
    • Masakazu KatsumataAyano TakeuchiKimiko Kazumura
    • G01N21/76
    • G01N21/77G01N21/6408G01N21/6486G01N2021/7786
    • The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value.
    • 本发明提供一种通过使用藻类来评价化学品的毒性的方法,其特征在于,包括:(a)通过加热来解冻冷冻藻类细胞的解冻步骤,并通过向其中加入培养基稀释所得细胞的悬浮液; (b)在解冻步骤(a)中培养获得的藻细胞以使藻类细胞从冷冻和解冻的作用中恢复的回收培养步骤; (c)在回收培养步骤(b)之后收集部分藻类细胞的确认步骤,通过向其中加入培养基稀释该部分藻类细胞,并测定其中的延迟发光的发光量 藻细胞作为初始值数据; (d)在确认步骤(c)之后将藻类细胞与含有测试物质的溶液混合以制备曝光样品并培养曝光样品的曝光步骤; (e)在曝光步骤(d)之后测量曝光样品的延迟发光的发光量作为曝光数据的测量步骤; 以及(f)基于初始值数据和曝光数据计算评价值,并根据评价值来评价被检物质的毒性的评价步骤。
    • 5. 发明授权
    • Harmful substance evaluating method and harmful substance evaluation kit
    • 有害物质评估方法和有害物质评估工具
    • US09448170B2
    • 2016-09-20
    • US10583128
    • 2004-12-16
    • Masakazu KatsumataHiroshi TsuchiyaTakashi KoikeMasataka Nishikawa
    • Masakazu KatsumataHiroshi TsuchiyaTakashi KoikeMasataka Nishikawa
    • C12Q1/02C12Q1/06G01N21/64
    • G01N21/6408G01N21/6486G01N2021/635
    • A biological growth inhibition factor assay method includes: a first step of mixing a photosynthetic sample, with an aqueous solution sample to prepare a test measurement solution, letting the test measurement solution stand, and then after illuminating light onto the test measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; a second step of mixing the photosynthetic sample with a standard sample, in which biological growth inhibition factors are not present, to prepare a standard measurement solution, letting the standard measurement solution stand, and then after illuminating light onto the standard measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; and a third step of computing assay values based on the light amounts of delayed fluorescence, respectively measured in the first step and the second step, and determining a comparison value of the assay values to assay biological growth inhibition factors. A biological growth inhibition factor assay method that enables analysis of a wide range of inhibition factors in a short time is thereby realized.
    • 生物生长抑制因子测定方法包括:将光合作用样品与水溶液样品混合以制备测试测量溶液的第一步骤,使测试测量溶液静置,然后将光照射到测试测量溶液上以达到预定的 照射时间,测量发射的延迟荧光的光量; 将光合作用样品与其中不存在生物生长抑制因子的标准样品混合以制备标准测量溶液,使标准测量溶液静置,然后将光照射到标准测量溶液上以达到预定的第二步骤 照射时间,测量发射的延迟荧光的光量; 以及分别基于第一步骤和第二步骤中测量的延迟荧光的光量来计算测定值的第三步骤,以及测定测定值以测定生物生长抑制因子的比较值。 从而实现了能够在短时间内分析广泛的抑制因子的生物生长抑制因子测定方法。
    • 9. 发明申请
    • Harmful Substance Evaluating Method and Harmful Substance Evaluation Kit
    • 有害物质评估方法及有害物质评估工具
    • US20070224659A1
    • 2007-09-27
    • US10583128
    • 2004-12-16
    • Masakazu KatsumataHiroshi TsuchiyaTakashi KoikeMasataka Nishikawa
    • Masakazu KatsumataHiroshi TsuchiyaTakashi KoikeMasataka Nishikawa
    • G01N21/64C12Q1/04G01N21/76
    • G01N21/6408G01N21/6486G01N2021/635
    • A biological growth inhibition factor assay method includes: a first step of mixing a photosynthetic sample, with an aqueous solution sample to prepare a test measurement solution, letting the test measurement solution stand, and then after illuminating light onto the test measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; a second step of mixing the photosynthetic sample with a standard sample, in which biological growth inhibition factors are not present, to prepare a standard measurement solution, letting the standard measurement solution stand, and then after illuminating light onto the standard measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; and a third step of computing assay values based on the light amounts of delayed fluorescence, respectively measured in the first step and the second step, and determining a comparison value of the assay values to assay biological growth inhibition factors. A biological growth inhibition factor assay method that enables analysis of a wide range of inhibition factors in a short time is thereby realized.
    • 生物生长抑制因子测定方法包括:将光合作用样品与水溶液样品混合以制备测试测量溶液的第一步骤,使测试测量溶液静置,然后将光照射到测试测量溶液上以达到预定的 照射时间,测量发射的延迟荧光的光量; 将光合作用样品与其中不存在生物生长抑制因子的标准样品混合以制备标准测量溶液,使标准测量溶液静置,然后将光照射到标准测量溶液上达预定的第二步骤 照射时间,测量发射的延迟荧光的光量; 以及分别基于第一步骤和第二步骤中测量的延迟荧光的光量来计算测定值的第三步骤,以及测定测定值以测定生物生长抑制因子的比较值。 从而实现了能够在短时间内分析广泛的抑制因子的生物生长抑制因子测定方法。