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1. 发明授权

US08798931B2 Evaluation method for evaluating a state of a photosynthesis sample 有权
标题翻译：用于评价光合作用样品的状态的评价方法
公开(公告)号：US08798931B2
公开(公告)日：2014-08-05
申请号：US12279789
申请日：2007-02-20
申请人： Masakazu Katsumata , Ryosuke Mochizuki , Hiroshi Satozono , Kimiko Kazumura , Ayano Takeuchi
发明人： Masakazu Katsumata , Ryosuke Mochizuki , Hiroshi Satozono , Kimiko Kazumura , Ayano Takeuchi
IPC分类号： G01N33/48 , G01N31/00 , G06G7/48 , G06G7/58
CPC分类号： G01N21/6486 , G01N21/6408 , G01N2021/635
摘要： An object of the present invention is to appropriately and easily evaluate a photosynthetic function of a photosynthetic sample contained in an evaluation sample.An evaluation method for photosynthetic sample according to the present invention is for evaluating a state of a photosynthetic sample with a photosynthetic function based on temporal data of a luminescence amount of delayed luminescence emitted from the photosynthetic sample. First, characteristic values that indicate characteristics are determined for a plurality of time periods in the temporal data. An evaluation value is then computed by weighting the characteristic values. The state of the photosynthetic sample is then evaluated based on the evaluation value.
摘要翻译：本发明的目的是适当且容易地评估评价样品中所含的光合作用样品的光合作用。根据本发明的光合作用样品的评价方法是基于从光合作用样品发出的延迟发光的发光量的时间数据来评价具有光合作用功能的光合作用样品的状态。首先，在时间数据中为多个时间段确定指示特性的特征值。然后通过加权特征值来计算评估值。然后根据评价值评价光合作用样品的状态。

2. 发明申请

US20130177967A1 METHOD FOR PREPARATION OF ALGAL CELLS, AND KIT FOR EVALUATION OF TOXICITY OF CHEMICAL SUBSTANCE 有权
标题翻译：制备ALGAL细胞的方法和用于评估化学物质毒性的试剂盒
公开(公告)号：US20130177967A1
公开(公告)日：2013-07-11
申请号：US13821326
申请日：2011-07-14
申请人： Kimiko Kazumura , Ayano Takeuchi , Masakazu Katsumata
发明人： Kimiko Kazumura , Ayano Takeuchi , Masakazu Katsumata
IPC分类号： G01N1/30
CPC分类号： G01N1/30 , C12N1/04 , C12N1/12 , C12Q1/025 , G01N2333/405
摘要： The present invention provides a method for preparing algal cells used for evaluating a toxicity of a chemical substance by delayed luminescence, the method comprising the step of freezing algal cells in a logarithmic growth phase.
摘要翻译：本发明提供一种制备用于通过延迟发光评价化学物质的毒性的藻类细胞的方法，该方法包括以对数生长期冷冻藻类细胞的步骤。

3. 发明申请

US20100159496A1 EVALUATION METHOD FOR PHOTOSYNTHESIS SAMPLE, EVALUATION SYSTEM FOR PHOTOSYNTHESIS SAMPLE, AND EVALUATION PROGRAM FOR PHOTOSYNTHESIS SAMPLE 有权
标题翻译：光合作用样品的评价方法，光合作用样品的评价系统和光合作用样品的评价程序
公开(公告)号：US20100159496A1
公开(公告)日：2010-06-24
申请号：US12279789
申请日：2007-02-20
申请人： Masakazu Katsumata , Ryosuke Mochizuki , Hiroshi Satozono , Kimiko Kazumura , Ayano Takeuchi
发明人： Masakazu Katsumata , Ryosuke Mochizuki , Hiroshi Satozono , Kimiko Kazumura , Ayano Takeuchi
IPC分类号： C12Q1/02
CPC分类号： G01N21/6486 , G01N21/6408 , G01N2021/635
摘要： An object of the present invention is to appropriately and easily evaluate a photosynthetic function of a photosynthetic sample contained in an evaluation sample.An evaluation method for photosynthetic sample according to the present invention is for evaluating a state of a photosynthetic sample with a photosynthetic function based on temporal data of a luminescence amount of delayed luminescence emitted from the photosynthetic sample. First, characteristic values that indicate characteristics are determined for a plurality of time periods in the temporal data. An evaluation value is then computed by weighting the characteristic values. The state of the photosynthetic sample is then evaluated based on the evaluation value.
摘要翻译：本发明的目的是适当且容易地评估评价样品中所含的光合作用样品的光合作用。根据本发明的光合作用样品的评价方法是基于从光合作用样品发出的延迟发光的发光量的时间数据来评价具有光合作用功能的光合作用样品的状态。首先，在时间数据中为多个时间段确定指示特性的特征值。然后通过加权特征值来计算评估值。然后根据评价值评价光合作用样品的状态。

4. 发明申请

US20120231491A1 METHOD FOR EVALUATING TOXICITY OF CHEMICAL USING ALGA 有权
标题翻译：使用ALGA评估化学毒性的方法
公开(公告)号：US20120231491A1
公开(公告)日：2012-09-13
申请号：US13508436
申请日：2010-10-26
申请人： Masakazu Katsumata , Ayano Takeuchi , Kimiko Kazumura
发明人： Masakazu Katsumata , Ayano Takeuchi , Kimiko Kazumura
IPC分类号： G01N21/76
CPC分类号： G01N21/77 , G01N21/6408 , G01N21/6486 , G01N2021/7786
摘要： The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value.
摘要翻译：本发明提供一种通过使用藻类来评价化学品的毒性的方法，其特征在于，包括：（a）通过加热来解冻冷冻藻类细胞的解冻步骤，并通过向其中加入培养基稀释所得细胞的悬浮液; （b）在解冻步骤（a）中培养获得的藻细胞以使藻类细胞从冷冻和解冻的作用中恢复的回收培养步骤; （c）在回收培养步骤（b）之后收集部分藻类细胞的确认步骤，通过向其中加入培养基稀释该部分藻类细胞，并测定其中的延迟发光的发光量藻细胞作为初始值数据; （d）在确认步骤（c）之后将藻类细胞与含有测试物质的溶液混合以制备曝光样品并培养曝光样品的曝光步骤; （e）在曝光步骤（d）之后测量曝光样品的延迟发光的发光量作为曝光数据的测量步骤; 以及（f）基于初始值数据和曝光数据计算评价值，并根据评价值来评价被检物质的毒性的评价步骤。

5. 发明授权

US09759639B2 Method for preparation of algal cells, and kit for evaluation of toxicity of chemical substance 有权
公开(公告)号：US09759639B2
公开(公告)日：2017-09-12
申请号：US13821326
申请日：2011-07-14
申请人： Kimiko Kazumura , Ayano Takeuchi , Masakazu Katsumata
发明人： Kimiko Kazumura , Ayano Takeuchi , Masakazu Katsumata
IPC分类号： G01N1/30 , C12N1/04 , C12N1/12 , C12Q1/02
CPC分类号： G01N1/30 , C12N1/04 , C12N1/12 , C12Q1/025 , G01N2333/405
摘要： The present invention provides a method for preparing algal cells used for evaluating a toxicity of a chemical substance by delayed luminescence, the method comprising the step of freezing algal cells in a logarithmic growth phase.

6. 发明授权

US08658392B2 Method for evaluating toxicity of chemical using alga 有权
标题翻译：使用藻类评价化学毒性的方法
公开(公告)号：US08658392B2
公开(公告)日：2014-02-25
申请号：US13508436
申请日：2010-10-26
申请人： Masakazu Katsumata , Ayano Takeuchi , Kimiko Kazumura
发明人： Masakazu Katsumata , Ayano Takeuchi , Kimiko Kazumura
IPC分类号： C12Q1/04
CPC分类号： G01N21/77 , G01N21/6408 , G01N21/6486 , G01N2021/7786
摘要： The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value.
摘要翻译：本发明提供一种通过使用藻类来评价化学品的毒性的方法，其特征在于，包括：（a）通过加热来解冻冷冻藻类细胞的解冻步骤，并通过向其中加入培养基稀释所得细胞的悬浮液; （b）在解冻步骤（a）中培养获得的藻细胞以使藻类细胞从冷冻和解冻的作用中恢复的回收培养步骤; （c）在回收培养步骤（b）之后收集部分藻类细胞的确认步骤，通过向其中加入培养基稀释该部分藻类细胞，并测定其中的延迟发光的发光量藻细胞作为初始值数据; （d）在确认步骤（c）之后将藻类细胞与含有测试物质的溶液混合以制备曝光样品并培养曝光样品的曝光步骤; （e）在曝光步骤（d）之后测量曝光样品的延迟发光的发光量作为曝光数据的测量步骤; 以及（f）基于初始值数据和曝光数据计算评价值，并根据评价值来评价被检物质的毒性的评价步骤。

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