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1. 发明授权

US5786219A Microspheres with fluorescent spherical zones 失效
标题翻译：具有荧光球形区域的微球
公开(公告)号：US5786219A
公开(公告)日：1998-07-28
申请号：US740184
申请日：1996-10-28
申请人： Yu-Zhong Zhang , Courtenay R. Kemper , Richard P. Haugland
发明人： Yu-Zhong Zhang , Courtenay R. Kemper , Richard P. Haugland
IPC分类号： G01N15/10 , G01N33/58 , G01Q60/18 , G01N33/546 , G01N33/533
CPC分类号： G01N15/1012 , G01N33/585 , Y10T428/2982 , Y10T428/2989 , Y10T428/2991 , Y10T428/2998
摘要： The invention describes novel fluorescently labeled microspheres, where the microspheres possess at least one internal fluorescent spherical zone. The invention also describes the method of preparing the novel microspheres, the method of calibrating microscopy instrumentation using the novel microspheres, the method of using the novel microspheres as distinct labels for combinatorial analysis and the use of the labeled microspheres as tagging agents and tracers.
摘要翻译：本发明描述了新颖的荧光标记微球，其中微球体具有至少一个内部荧光球形区域。本发明还描述了制备新型微球的方法，使用新型微球校准显微镜仪器的方法，使用新型微球作为组合分析的不同标签的方法，以及使用标记的微球作标记剂和示踪剂。

2. 发明申请

US20060045814A1 Microplates containing microsphere fluorescence standards, microsphere standards, and methods for their use 审中-公开
标题翻译：含微球荧光标准品，微球标准品及其使用方法的微孔板
公开(公告)号：US20060045814A1
公开(公告)日：2006-03-02
申请号：US11218032
申请日：2005-08-31
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： B01L9/00
CPC分类号： B01L3/5085 , B01L2200/148 , B01L2300/0609 , B01L2300/0829 , G02B21/0076
摘要： Microplates containing spherical “microsphere” fluorescence standards are disclosed. The microplates can be prepared using several methods including airbrushing, application with an inkjet printer, or controlled evaporation. Spherical bead standards containing two or more regions stained with dyes of different fluorescence lifetimes, and methods for their preparation are also disclosed. The microplates can be used as calibration standards for fluorescence and confocal microscopes, and as calibration tools for microscope-based high content screening (“HCS”) instruments.
摘要翻译：公开了含有球形“微球”荧光标准品的微孔板。微孔板可以使用几种方法制备，包括用喷墨打印机进行喷涂，用喷墨打印机或受控蒸发。还公开了包含用不同荧光寿命的染料染色的两个或更多个区域的球形珠标准及其制备方法。微孔板可用作荧光和共焦显微镜的校准标准，也可用作基于显微镜的高含量筛选（“HCS”）仪器的校准工具。

3. 发明授权

US5362628A Fluorescent haloalkyl derivatives of reporter molecules well retained in cells 失效
标题翻译：报道分子的荧光卤代烷基衍生物保留在细胞中
公开(公告)号：US5362628A
公开(公告)日：1994-11-08
申请号：US26633
申请日：1993-03-05
申请人： Richard P. Haugland , Yu-Zhong Zhang , Ram Sabnis , Nels A. Olson , John J. Naleway , Rosaria P. Haugland
发明人： Richard P. Haugland , Yu-Zhong Zhang , Ram Sabnis , Nels A. Olson , John J. Naleway , Rosaria P. Haugland
IPC分类号： C12Q1/04 , C12Q1/34 , G01N33/535 , C12Q1/00 , A61K37/02 , C07H15/24 , G01N33/40
CPC分类号： C12Q1/04 , C12Q1/34 , G01N33/535 , C12Q2334/00 , C12Q2334/20 , C12Q2334/40 , C12Q2337/20 , G01N2333/924 , Y10S530/802
摘要： The subject invention provides a method for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process involving conjugation of haloalkyl-substituted derivatives of a reporter molecule with intracellular cysteine-containing peptides while unblocking the reporter molecule. The method for analyzing metabolic activity of cells involves the use of a substrate having the formXR-REPORTER-BLOCKwherein -BLOCK is a group selected to be removable by action of a specific analyte, to give REPORTER spectral properties different from those of the substrate,-REPORTER- is a molecule that, when no longer bound to BLOCK by a BLOCK-REPORTER bond, has spectral properities different from those of the substrate, andXR-- is a haloalkyl moiety that can covalently react with an intracellular thiol (Z--S--H) to form a thioether conjugate (Z--S--R--).After the substrate enters the cells, the analyte removes BLOCK to make REPORTER detectable by the change in spectral properties, and the haloalkyl XR reacts with the intracellular thiol to form the thioether conjugate inside the cells, which is well-retained in the cells.
摘要翻译：本发明提供了通过仅在存在特定酶的完整细胞中改善可检测报道分子的保留来分析细胞中的代谢活性的方法。特别地，涉及将报道分子的卤代烷基取代的衍生物与含细胞内半胱氨酸的肽结合的两部分方法的改进的保留结果，同时解除报道分子的阻断。用于分析细胞代谢活性的方法涉及使用具有XR-REPORTER-BLOCK形式的底物，其中-BLOCK是通过特定分析物的作用被选择为可去除的基团，以产生与底物的不同的REPORTER光谱性质 -REPORTER-是一个分子，当不再通过BLOCK-REPORTER键与BLOCK结合时，具有与底物不同的光谱性质，XR-是可以与胞内硫醇（ZSH）共价反应的卤代烷基部分，以形成硫醚缀合物（ZSR-）。在底物进入细胞后，分析物除去BLOCK以通过光谱性质的变化使REPORTER可检测到，并且卤代烷基XR与细胞内硫醇反应以在细胞内形成硫醚缀合物，其在细胞中良好保留。

4. 发明授权

US08304242B2 Methods for detecting defects in inorganic-coated polymer surfaces 有权
标题翻译：检测无机涂层聚合物表面缺陷的方法
公开(公告)号：US08304242B2
公开(公告)日：2012-11-06
申请号：US12808325
申请日：2008-12-17
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： G01N31/22
CPC分类号： G01N21/91 , G01N21/643 , G01N21/6489 , G01N21/94 , G01N2021/646 , G01N2021/8427
摘要： Lipophilic fluorescent substances can be used to detect surface defects in materials having hydrophilic (e.g., inorganic) coatings. Use of the described methods makes surface defects appear fluorescent, while the remaining surfaces are not labeled. The disclosed methods are inexpensive, rapid, and easy alternatives to existing approaches.
摘要翻译：亲脂性荧光物质可用于检测具有亲水（例如无机）涂层的材料中的表面缺陷。使用所述方法会使表面缺陷出现荧光，而其余表面未标记。所公开的方法是便宜，快速和容易的替代现有方法。

5. 发明申请

US20120276015A1 Optical in vivo Imaging Contrast Agents and Methods of Use 审中-公开
标题翻译：光学体内成像对比剂和使用方法
公开(公告)号：US20120276015A1
公开(公告)日：2012-11-01
申请号：US13344224
申请日：2012-01-05
申请人： John Mauro , Julie Nyhus , Thomas Steinberg , Yu-Zhong Zhang
发明人： John Mauro , Julie Nyhus , Thomas Steinberg , Yu-Zhong Zhang
IPC分类号： A61K49/00
CPC分类号： A61B5/0059 , A61K49/0021 , A61K49/0093
摘要： Provided is an optical in vivo contrast agent comprising a fluorescent polymeric microsphere, wherein the micro-sphere is impregnated with a dye having an excitation and emission spectrum compatible with in vivo imaging, and wherein the microsphere is coated with a block copolymer.
摘要翻译：提供了一种包含荧光聚合物微球的光学体内造影剂，其中所述微球浸渍有具有与体内成像相容的激发和发射光谱的染料，并且其中所述微球涂覆有嵌段共聚物。

6. 发明申请

US20080019921A1 UNIFORM FLUORESCENT MICROSPHERE WITH HYDROPHOBIC SURFACES 审中-公开
标题翻译：具有疏水性表面的均匀荧光显微镜
公开(公告)号：US20080019921A1
公开(公告)日：2008-01-24
申请号：US11772063
申请日：2007-06-29
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： A61K49/00
CPC分类号： A61K49/0021 , A61K49/0004 , A61K49/0093
摘要： Fluorescent microspheres for the measurement of blood flow are provided. The microspheres are substantially uniform in diameter and have a hydrophobic surface, which allows them to circulate more freely throughout bloodstream, while reducing immunogenicity, particle aggregation and bioaccumulation. The hydrophobic surface on each microsphere is generally comprised of polymeric material having a limited surface charge.
摘要翻译：提供了用于测量血流量的荧光微球。微球的直径基本上是均匀的并且具有疏水性表面，这允许它们在血流中更自由地循环，同时降低免疫原性，颗粒聚集和生物累积。每个微球体上的疏水表面通常由具有有限表面电荷的聚合物材料组成。

7. 发明授权

US5869689A Stains for acidic organelles 失效
标题翻译：酸性细胞器污渍
公开(公告)号：US5869689A
公开(公告)日：1999-02-09
申请号：US544226
申请日：1995-10-17
申请人： Yu-Zhong Zhang , Zhenjun Diwu , Richard P. Haugland
发明人： Yu-Zhong Zhang , Zhenjun Diwu , Richard P. Haugland
IPC分类号： C07F5/02 , G01N1/30 , G01N33/58 , C07D207/00
CPC分类号： G01N33/582 , C07F5/022 , G01N1/30
摘要： Dipyrrometheneboron difluoride dyes possessing a covalently attached basic amine moiety are described that have utility for staining acidic organelles. Samples comprising isolated acidic organelles, or a cell or cells containing acidic organelles, are stained by preparing an aqueous labeling solution of the dye and incubating the sample in the labeling solution for a time sufficient to produce fluorescent labeled acidic organelles.
摘要翻译：描述了具有共价连接的碱性胺部分的二吡咯亚甲基二氟化物染料，其具有用于染色酸性细胞器的效用。包含分离的酸性细胞器或含有酸性细胞器的细胞或细胞的样品通过制备染料的水性标记溶液并将样品在标记溶液中孵育足以产生荧光标记的酸性细胞器的时间来染色。

8. 发明授权

US09567346B2 Biotin derivatives 有权
标题翻译：生物素衍生物
公开(公告)号：US09567346B2
公开(公告)日：2017-02-14
申请号：US13881518
申请日：2011-10-28
申请人： Lai-Qiang Ying , Bruce Branchaud , Yu-Zhong Zhang , Stephen Yue
发明人： Lai-Qiang Ying , Bruce Branchaud , Yu-Zhong Zhang , Stephen Yue
IPC分类号： C07D495/04 , C08B37/00 , G01N33/532 , G01N33/543 , G01N33/58
CPC分类号： C07D495/04 , C07B2200/11 , C07D403/12 , C07K1/13 , C07K1/22 , C07K16/42 , C07K2317/20 , C07K2317/92 , C08B37/00 , C09B11/24 , C09K11/06 , C09K2211/1007 , C09K2211/1088 , C12N5/0636 , C12N5/0637 , C12N5/0638 , G01N33/532 , G01N33/533 , G01N33/54353 , G01N33/58 , G01N33/582 , G01N33/6803
摘要： Biotin derivatives, methods of using the biotin derivatives and kits comprising the biotin derivatives.
摘要翻译：生物素衍生物，使用生物素衍生物的方法和包含生物素衍生物的试剂盒。

9. 发明申请

US20130020507A1 Methods for Detecting Defects in Inorganic-Coated Polymer Surfaces 审中-公开
标题翻译：检测无机涂层聚合物表面缺陷的方法
公开(公告)号：US20130020507A1
公开(公告)日：2013-01-24
申请号：US13633713
申请日：2012-10-02
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： G01N21/64 , B32B3/30 , C07F5/02
CPC分类号： G01N21/91 , C07F5/022 , Y10T428/24355
摘要： Lipophilic fluorescent substances can be used to detect surface defects in materials having hydrophilic (e.g., inorganic) coatings. Use of the described methods makes surface defects appear fluorescent, while the remaining surfaces are not labeled. The disclosed methods are inexpensive, rapid, and easy alternatives to existing approaches.
摘要翻译：亲脂性荧光物质可用于检测具有亲水（例如无机）涂层的材料中的表面缺陷。使用所述方法会使表面缺陷出现荧光，而其余表面未标记。所公开的方法是便宜，快速和容易的替代现有方法。

10. 发明申请

US20120064012A1 Uniform Fluorescent Microspheres with Hydrophobic Surfaces 审中-公开
标题翻译：均匀荧光微球与疏水表面
公开(公告)号：US20120064012A1
公开(公告)日：2012-03-15
申请号：US13209770
申请日：2011-08-15
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： A61K49/00
CPC分类号： A61K49/0021 , A61K49/0004 , A61K49/0093
摘要： Fluorescent microspheres for the measurement of blood flow are provided. The microspheres are substantially uniform in diameter and have a hydrophobic surface, which allows them to circulate more freely throughout bloodstream, while reducing immunogenicity, particle aggregation and bioaccumulation. The hydrophobic surface on each microsphere is generally comprised of polymeric material having a limited surface charge.
摘要翻译：提供了用于测量血流量的荧光微球。微球的直径基本上是均匀的并且具有疏水性表面，这允许它们在血流中更自由地循环，同时降低免疫原性，颗粒聚集和生物累积。每个微球体上的疏水表面通常由具有有限表面电荷的聚合物材料组成。

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