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1. 发明授权

US07641858B2 Apparatus for introducing fluid into microfluidic chip by using centrifugal force, a system including the apparatus, and a method of using the apparatus 有权
标题翻译：用于通过离心力将流体引入微流体芯片的装置，包括该装置的系统以及使用该装置的方法
公开(公告)号：US07641858B2
公开(公告)日：2010-01-05
申请号：US11846794
申请日：2007-08-29
申请人： Young-rok Kim , Jun-hong Min , Kak Namkoong , Kwang-ho Cheong , Chang-eun Yoo , Ki-woong Han , Ki-eun Kim
发明人： Young-rok Kim , Jun-hong Min , Kak Namkoong , Kwang-ho Cheong , Chang-eun Yoo , Ki-woong Han , Ki-eun Kim
IPC分类号： B01L3/14 , B65B3/04
CPC分类号： B01L9/527 , B01L3/5021 , B01L3/5027 , B01L2200/027 , B01L2300/0832 , B01L2400/0409 , C12M23/16
摘要： An apparatus introducing a fluid using a centrifugal force includes an introduction member including a chip receiver and a fluid introduction reservoir, the chip receiver receiving a first part of a microfluidic chip, the first part including an inlet, the fluid introduction reservoir storing a fluid to be introduced to the microfluidic chip, the fluid introduction reservoir having an exit formed to correspond to the inlet of the microfluidic chip received in the chip receiver, and a support member supporting a second part of the microfluidic chip, wherein the microfluidic chip is disposed between the introduction member and the support member, the apparatus is rotatable in a state where the introduction member is closer to a center of rotation than the microfluidic chip, and the fluid is introducible from the fluid introduction reservoir through the inlet into the microfluidic chip due to a centrifugal force generated by rotation.
摘要翻译：使用离心力引入流体的装置包括引入构件，其包括芯片接收器和流体引入储存器，所述芯片接收器接收微流体芯片的第一部分，所述第一部分包括入口，所述流体引入储存器将流体存储到引入到微流体芯片中，流体引入储存器具有形成为对应于接收在芯片接收器中的微流体芯片的入口的出口，以及支撑微流体芯片的第二部分的支撑构件，其中微流体芯片设置在引导构件和支撑构件，该装置可以在导入构件比微流体芯片更靠近旋转中心的状态下旋转，并且流体可以从流体引入储存器通过入口引入到微流体芯片中，这是由于由旋转产生的离心力。

2. 发明授权

US07807360B2 Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber 有权
标题翻译：在单微室中浓缩和扩增核酸的方法和装置
公开(公告)号：US07807360B2
公开(公告)日：2010-10-05
申请号：US11620961
申请日：2007-01-08
申请人： Young-rok Kim , Jun-hong Min , In-ho Lee , Young-sun Lee , Chang-eun Yoo , Ki-woong Han
发明人： Young-rok Kim , Jun-hong Min , In-ho Lee , Young-sun Lee , Chang-eun Yoo , Ki-woong Han
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/5027 , B01L3/5088 , C12Q1/686 , C12Q2565/629 , C12Q2537/149 , C12Q2527/125
摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.
摘要翻译：在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可染色盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低通过消除样品的运输而损失。因此容易提供用于浓缩和扩增核酸的完整的自动化系统。

3. 发明申请

US20070238114A1 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US20070238114A1
公开(公告)日：2007-10-11
申请号：US11639794
申请日：2006-12-15
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12N1/08
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

4. 发明授权

US08658359B2 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US08658359B2
公开(公告)日：2014-02-25
申请号：US13079926
申请日：2011-04-05
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/00
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

5. 发明授权

US07955801B2 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US07955801B2
公开(公告)日：2011-06-07
申请号：US11639794
申请日：2006-12-15
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

6. 发明授权

US07855069B2 Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权
标题翻译：使用微磁珠和激光快速破坏细胞或病毒的方法和装置
公开(公告)号：US07855069B2
公开(公告)日：2010-12-21
申请号：US11253541
申请日：2005-10-19
申请人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
发明人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
IPC分类号： C12M1/42 , C12M1/33
CPC分类号： C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
摘要： A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
摘要翻译：提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠将振动有效地转移到细胞中，通过疏水处理芯片的内表面和细胞来解决粗糙表面上的微流体问题裂解芯片可应用于LOC。

7. 发明申请

US20060084165A1 Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权
标题翻译：使用微磁珠和激光快速破坏细胞或病毒的方法和装置
公开(公告)号：US20060084165A1
公开(公告)日：2006-04-20
申请号：US11253541
申请日：2005-10-19
申请人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
发明人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
IPC分类号： C12N1/06
CPC分类号： C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
摘要： A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
摘要翻译：提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决裂解芯片可应用于LOC。

8. 发明授权

US07838036B2 Method and apparatus for isolating and purifying nucleic acid using a single surface 有权
公开(公告)号：US07838036B2
公开(公告)日：2010-11-23
申请号：US12716695
申请日：2010-03-03
申请人： Chang-eun Yoo , Sung-young Jeong , Young-rok Kim
发明人： Chang-eun Yoo , Sung-young Jeong , Young-rok Kim
IPC分类号： A61K9/14 , A61K9/16 , C12Q1/68 , C07H21/04 , C12Q1/70
CPC分类号： B01J20/28009 , B01D15/1807 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/327 , C08F20/02 , C08F26/06 , C12N1/066 , C12N13/00
摘要： Provided is a method of isolating nucleic acid from cells using a single surface, wherein a compound represented by Formula 1 is bound to the surface. Also provided are an apparatus for isolation of nucleic acids, and a bead for isolating nucleic acids.

9. 发明申请

US20060134675A1 Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
标题翻译：使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法
公开(公告)号：US20060134675A1
公开(公告)日：2006-06-22
申请号：US11297783
申请日：2005-12-07
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C07H21/04
摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.
摘要翻译：提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

10. 发明授权

US07803539B2 Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
标题翻译：使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法
公开(公告)号：US07803539B2
公开(公告)日：2010-09-28
申请号：US11297783
申请日：2005-12-07
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12M1/34 , C07H21/00
CPC分类号： C07H21/04
摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.
摘要翻译：提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

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