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    • 1. 发明授权
    • Method for continuous reaction with fluidized immobilized lipase
    • 与流化固定化脂肪酶连续反应的方法
    • US5010004A
    • 1991-04-23
    • US255599
    • 1988-10-11
    • Yoshitsugu KosugiHideoki TanakaHideo SuzukiMasaru Shiraki
    • Yoshitsugu KosugiHideoki TanakaHideo SuzukiMasaru Shiraki
    • C12P7/64C12M1/40
    • C12M23/34C12M21/18C12M23/02C12M25/20C12M27/00C12M47/10Y10S435/813
    • A water-soluble substrate and an oily substrate are continuously reacted with immobilized lipase in a reaction vessel having vertically maintained apart upper and lower conically-shaped regions, respectively, for separation of a water-soluble product and an oily product, a plurality of lipase reaction zones each containing immobilized lipase capable of being fluidized and an agitating means, and a plurality of intermediate separation zones for separation of an oily substance and a water-soluble substance. The lipase reaction zones and the intermediate separation zones are disposed alternately between the upper and lower conically-shaped separation regions. Boundaries between the lipase reaction zones and intermediate separation zones are pervious to liquid but impervious to the immobilized lipase. The water-soluble substrate and oily substrate are passed in counterflow contact through the lipase reaction zones and intermediate separation zones and mutually contact the immobilized lipase which has been fluidized. An oily product is recovered from the upper conically-shaped separation region and a water-soluble product is recovered from the lower conically-shaped separation region.
    • 水溶性底物和油性底物分别在具有垂直保持分开的上下圆锥形区域的反应容器中与固定化脂肪酶连续反应,用于分离水溶性产物和油性产物,多种脂肪酶 每个含有能够流化的固定化脂肪酶的反应区和搅拌装置,以及用于分离油性物质和水溶性物质的多个中间分离区。 脂肪酶反应区和中间分离区交替设置在上下圆锥形分离区之间。 脂肪酶反应区和中间分离区之间的边界可以渗透液体,但对固定的脂肪酶是不透水的。 水溶性底物和油性底物通过反向流动接触通过脂肪酶反应区和中间分离区,并与已流化的固定化脂肪酶相互接触。 从上部圆锥形分离区域回收油状产物,并从下锥形分离区域回收水溶性产物。