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1. 发明授权

US07855273B2 Method for manufacturing gelatin with reduced endotoxin content and low endotoxin gelatin 有权
标题翻译：降低内毒素含量和低内毒素明胶制造明胶的方法
公开(公告)号：US07855273B2
公开(公告)日：2010-12-21
申请号：US11681323
申请日：2007-03-02
申请人： Yoshitaka Kanayama , Yasuo Sakai
发明人： Yoshitaka Kanayama , Yasuo Sakai
IPC分类号： C07K14/78 , C07K1/14
CPC分类号： A61L27/222 , A61K9/0024 , C08H1/06 , C09H3/02
摘要： A method of manufacturing gelatin with reduced endotoxin content. The method comprises processing a starting material gelatin-containing solution containing gelatin and an endotoxin with an ultrafiltration film having a molecular weight cut-off falling within a range of from 20,000 to 300,000 and having a molecular weight cut-off capable of passing at least a portion of the gelatin contained in the starting material gelatin-containing solution to obtain a permeate that is a gelatin-containing solution with a reduced endotoxin content. A gelatin having an average molecular weight falling within a range of 1,000 to 300,000 and an endotoxin content of less than 1 EU/mL per 1.0 percent of protein.
摘要翻译：制造具有降低的内毒素含量的明胶的方法。该方法包括用分子量截留值为20,000〜300,000的超滤膜加工含有明胶和内毒素的原料明胶溶液，并具有分子量截留能够通过至少一种含有起始原料明胶溶液中的明胶的一部分，以获得具有降低的内毒素含量的含有明胶的溶液的渗透物。平均分子量在1000至300,000范围内，每1.0％蛋白质的内毒素含量小于1EU / mL的明胶。

2. 发明申请

US20060134749A1 Protease, DNA encoding the same, and method for manufacturing protease 有权
标题翻译：蛋白酶，编码相同的DNA，以及制造蛋白酶的方法
公开(公告)号：US20060134749A1
公开(公告)日：2006-06-22
申请号：US11287307
申请日：2005-11-28
申请人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
发明人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
IPC分类号： C07H21/04 , C12P21/06 , C12N9/64
CPC分类号： C12N9/52
摘要： The present invention provides a protease, DNA encoding this protease, a recombinant vector comprising this DNA, and a method for manufacturing the protease using the transformant.
摘要翻译：本发明提供蛋白酶，编码该蛋白酶的DNA，包含该DNA的重组载体，以及使用该转化体制造蛋白酶的方法。

3. 发明申请

US20100015664A1 PROTEASE, DNA ENCODING THE SAME, AND METHOD FOR MANUFACTURING PROTEASE 有权
标题翻译： PROTEASE，编码其的DNA，以及制造蛋白质的方法
公开(公告)号：US20100015664A1
公开(公告)日：2010-01-21
申请号：US12537259
申请日：2009-08-07
申请人： Yoshitaka KANAYAMA , Yasuo Sakai , Takahiro Hongo
发明人： Yoshitaka KANAYAMA , Yasuo Sakai , Takahiro Hongo
IPC分类号： C12P21/02 , C12N15/11 , C12N1/20 , C12N1/21
CPC分类号： C12N9/52
摘要： A protease having the following characteristics: (i) a molecular weight of 21,000±1,000 (by SDS-PAGE) and (ii) specific protease activity cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (1) below, cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (2) below, or cutting the Ser-Leu site of a peptide or protein comprising amino acid sequence (3) below: Pro-Gln-Gly-Phe-Gln-Gly-Pro(1) (SEQ ID NO: 4) Pro-Ala-Gly-Phe-Ala-Gly-Pro(2) (SEQ ID NO: 5) Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro.(3) (SEQ ID NO: 6) DNA encoding this protease, including vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.
摘要翻译：具有以下特征的蛋白酶：（i）分子量为21,000±1,000（通过SDS-PAGE）和（ii）切割包含以下氨基酸序列（1）的肽或蛋白质的Gly-Phe位点的特异性蛋白酶活性，切割包含以下氨基酸序列（2）的肽或蛋白质的Gly-Phe位点，或切割包含以下氨基酸序列（3）的肽或蛋白质的Ser-Leu位点：Pro-Gln-Gly-Phe-Gln -Gly-Pro（1）（SEQ ID NO：4）Pro-Ala-Gly-Phe-Ala-Gly-Pro（2）（SEQ ID NO：5）Gln-Thr-Gln-Ser-Leu-Val-Thr -Pro。（3）（SEQ ID NO：6）编码该蛋白酶的DNA，包含含有该DNA的载体，以及通过用该DNA转化细胞产生该蛋白酶的方法。

4. 发明授权

US07601807B2 Protease, DNA encoding the same, and method for manufacturing protease 有权
标题翻译：蛋白酶，编码相同的DNA，以及制造蛋白酶的方法
公开(公告)号：US07601807B2
公开(公告)日：2009-10-13
申请号：US11287307
申请日：2005-11-28
申请人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
发明人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
IPC分类号： C07K1/00
CPC分类号： C12N9/52
摘要： A protease having the following characteristics: (i) a molecular weight of 21,000±1,000 (by SDS-PAGE) and (ii) specific protease activity cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (1) below, cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (2) below, or cutting the Ser-Leu site of a peptide or protein comprising amino acid sequence (3) below: (1) (SEQ ID NO:4) Pro-Gln-Gly-Phe-Gln-Gly-Pro (2) (SEQ ID NO:5) Pro-Ala-Gly-Phe-Ala-Gly-Pro (3) (SEQ ID NO:6) Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro. DNA encoding this protease, including vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.
摘要翻译：具有以下特征的蛋白酶：（i）分子量为21,000±1,000（通过SDS-PAGE）和（ii）切割包含以下氨基酸序列（1）的肽或蛋白质的Gly-Phe位点的特异性蛋白酶活性，切割包含以下氨基酸序列（2）的肽或蛋白质的Gly-Phe位点，或切割包含以下氨基酸序列（3）的肽或蛋白质的Ser-Leu位点：（1）（SEQ ID NO：4 ）Pro-Gln-Gly-Phe-Gln-Gly-Pro（2）（SEQ ID NO：5）Pro-Ala-Gly-Phe-Ala-Gly-Pro（3）（SEQ ID NO：6）Gln-Thr -Gln-Ser-Leu-Val-Thr-Pro。编码该蛋白酶的DNA，包括含有该DNA的载体，以及通过用该DNA转化细胞来生产该蛋白酶的方法。

5. 发明授权

US07871797B2 DNA encoding a bacterial protease for degrading gelatin and collagen 有权
标题翻译：编码细菌蛋白酶降解凝胶和胶原的DNA
公开(公告)号：US07871797B2
公开(公告)日：2011-01-18
申请号：US12537259
申请日：2009-08-07
申请人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
发明人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
IPC分类号： C12P21/02
CPC分类号： C12N9/52
摘要： The present invention relates to a protease having a molecular weight of 21,000±1,000 (by SDS-PAGE) and a specific protease activity capable of cutting at the Gly-Phe site of a peptide or protein comprising the amino acid sequence Pro-Gln-Gly-Phe-Gln-Gly-Pro or Pro-Ala-Gly-Phe-Ala-Gly-Pro or cutting at the Ser-Leu site of a peptide or protein comprising the amino acid sequence Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro. The invention further provides DNA encoding this protease, as well as vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.
摘要翻译：本发明涉及分子量为21,000±1,000（通过SDS-PAGE）的蛋白酶和能够在包含氨基酸序列Pro-Gln-Gly的肽或蛋白质的Gly-Phe位点切割的特异性蛋白酶活性 -Phe-Gln-Gly-Pro或Pro-Ala-Gly-Phe-Ala-Gly-Pro或在包含氨基酸序列Gln-Thr-Gln-Ser-Leu- Val-Thr-Pro。本发明还提供了编码该蛋白酶的DNA，以及含有该DNA的载体，以及通过用该DNA转化细胞来生产该蛋白酶的方法。

6. 发明申请

US20080132682A1 METHOD FOR MANUFACTURING GELATIN WITH REDUCED ENDOTOXIN CONTENT AND LOW ENDOTOXIN GELATIN 有权
标题翻译：降低内毒素含量和低内毒素凝胶的制备方法
公开(公告)号：US20080132682A1
公开(公告)日：2008-06-05
申请号：US11681323
申请日：2007-03-02
申请人： Yoshitaka Kanayama , Yasuo Sakai
发明人： Yoshitaka Kanayama , Yasuo Sakai
IPC分类号： C07K14/78 , C07K1/14
CPC分类号： A61L27/222 , A61K9/0024 , C08H1/06 , C09H3/02
摘要： A method of manufacturing gelatin with reduced endotoxin content. The method comprises processing a starting material gelatin-containing solution containing gelatin and an endotoxin with an ultrafiltration film having a molecular weight cut-off falling within a range of from 20,000 to 300,000 and having a molecular weight cut-off capable of passing at least a portion of the gelatin contained in the starting material gelatin-containing solution to obtain a permeate that is a gelatin-containing solution with a reduced endotoxin content. A gelatin having an average molecular weight falling within a range of 1,000 to 300,000 and an endotoxin content of less than 1 EU/mL per 1.0 percent of protein.
摘要翻译：制造具有降低的内毒素含量的明胶的方法。该方法包括用分子量截留值为20,000〜300,000的超滤膜加工含有明胶和内毒素的原料明胶溶液，并具有分子量截留能够通过至少一种含有起始原料明胶溶液中的明胶的一部分，以获得具有降低的内毒素含量的含有明胶的溶液的渗透物。平均分子量在1000至300,000范围内，每1.0％蛋白质的内毒素含量小于1EU / mL的明胶。

7. 发明申请

US20110026679A1 X-RAY METERING APPARATUS, AND X-RAY METERING METHOD 有权
标题翻译： X射线测距仪，X射线测距仪
公开(公告)号：US20110026679A1
公开(公告)日：2011-02-03
申请号：US12667500
申请日：2008-07-01
申请人： Hiroyuki Nose , Daisuke Ishida , Namio Kaneko , Yasuo Sakai , Mitsuru Uesaka , Fumito Sakamoto , Katsuhiro Dobashi
发明人： Hiroyuki Nose , Daisuke Ishida , Namio Kaneko , Yasuo Sakai , Mitsuru Uesaka , Fumito Sakamoto , Katsuhiro Dobashi
IPC分类号： H05G2/00
CPC分类号： H05G2/00
摘要： An X-ray waveform is generated by validating detection data corresponding to when an X-ray (4) is generated at a collision point (9) among X-ray detection data and invalidating other data. For example, when laser light (3) is pulse laser light and an electron beam (1) is a continuous electron beam or a pulse-like electron beam having a pulse width equal to or greater than that of the pulse laser light, the X-ray waveform is generated by detecting the laser light (3) and multiplying the X-ray detection data by laser light detection data after making time axes coincident with respect to the collision point (9).
摘要翻译：通过验证与在X射线检测数据中的碰撞点（9）生成X射线（4）并使其他数据无效的检测数据，生成X射线波形。例如，当激光（3）是脉冲激光并且电子束（1）是连续的电子束或具有等于或大于脉冲激光的脉冲宽度的脉冲状电子束时，X 通过检测激光（3）并且在使相对于碰撞点（9）重合的时间轴之后乘以激光检测数据来生成X射线波形。

8. 发明授权

US07596937B2 Rubber product-reinforcing metallic cord and method for manufacturing such a cord 有权
标题翻译：橡胶制品增强金属帘线及其制造方法
公开(公告)号：US07596937B2
公开(公告)日：2009-10-06
申请号：US11547717
申请日：2005-04-07
申请人： Kenichi Okamoto , Yasuo Sakai , Kazuhiko Saito
发明人： Kenichi Okamoto , Yasuo Sakai , Kazuhiko Saito
IPC分类号： D02G3/22 , D02G3/48
CPC分类号： D07B3/02 , B60C9/0007 , D07B1/0613 , D07B1/062 , D07B3/10 , D07B2201/104 , D07B2201/2023 , D07B2201/2024 , D07B2201/2038 , D07B2207/205 , D07B2207/4009 , D07B2207/4013 , D07B2501/2046 , D07B2801/12
摘要： A rubber product-reinforcing metallic cord is provided which comprises three to five metallic filaments, which shows a high degree of penetration of rubber without reducing compressive rigidity, and which can be manufactured at low facility and production costs. A rubber product-reinforcing metallic cord 7 is formed by twisting together a pre-strand 6 comprising a first metallic filament 1 and a second metallic filament 2 helically wrapped around the first filament 1, and a fifth metallic filament 3 (or 5) with a twist pitch P. The metallic cord 7 may be formed by twisting together two of such pre-strands, or by twisting together two of such pre-strands and the fifth metallic filament.
摘要翻译：提供一种橡胶制品增强金属帘线，其包括三至五根金属丝，其显示出高度的橡胶渗透性，而不降低抗压刚度，并且可以以低的设备和生产成本制造。通过将包括第一金属细丝1和螺旋缠绕在第一丝1周围的第二金属细丝2的预捻线6和第五金属丝3（或5）与第一金属细丝3 扭曲节距P.金属帘线7可以通过将两根这样的预股线捻合在一起而形成，或者通过将两条这样的预股线和第五金属丝捻合在一起而形成。

9. 发明申请

US20090025846A1 Metal Cord, Rubber-Cord Complex and Pneumatic Tire Using the Same 审中-公开
标题翻译：金属线，橡胶线复合体和使用其的气动轮胎
公开(公告)号：US20090025846A1
公开(公告)日：2009-01-29
申请号：US12085546
申请日：2006-11-30
申请人： Shinichi Miyazaki , Junichi Kodama , Yasuo Sakai , Keisuke Aramaki , Yuichi Sano , Kenichi Okamoto
发明人： Shinichi Miyazaki , Junichi Kodama , Yasuo Sakai , Keisuke Aramaki , Yuichi Sano , Kenichi Okamoto
IPC分类号： B60C9/00
CPC分类号： B60C9/0007 , B60C9/2006 , C23C2/26 , C23C10/02 , C23C28/021 , C23C28/023 , C23C28/025 , C25D5/10 , C25D5/50 , C25D7/0607 , D07B1/0666 , D07B2201/2011 , D07B2205/3089 , D07B2401/2035 , D07B2401/2095 , D07B2501/2046 , D07B2501/2076 , D07B2801/18
摘要： A rubber-cord complex 9 having an improved wet heat adhesive property between a rubber and a cord comprising a drawn plated wire, and including a cord 10 comprising a drawn plated wire 17 prepared by providing a brass plated layer 16E on the surface of an element wire 15 and drawing the resulting plated wire, and a rubber 12 vulcanized and bonded to the cord 10, wherein the rubber-cord complex 9 has an adhesion reaction layer 25 formed by a cross-linking reaction of sulfur and copper, between the rubber 12 and the brass plated layer 16E, and in a wet heat deterioration state after being subjected to the vulcanization to bond the rubber 12 thereto and further held under an atmosphere having a temperature of 50 to 100° C. and a humidity of 60 to 100% for one hour to 20 days, the adhesion reaction layer 25 has an average thickness of 50 to 1,000 nm, and the interface S between the adhesion reaction layer 25 and the rubber has a fractal dimension of 1.001 to 1.300.
摘要翻译：一种橡胶帘线配合体9，其在橡胶和帘线之间具有改善的湿热粘合性，其包括拉制的电镀线，并且包括帘线10，帘线10包括通过在元件表面上提供黄铜镀层16E而制备的拉制电镀线17 线15并拉制所得到的电镀线，以及硫化并结合到帘线10的橡胶12，其中橡胶帘线复合体9具有通过硫和铜的交联反应形成的粘附反应层25，橡胶12 黄铜镀层16E，经过硫化后的湿热退化状态，将橡胶12粘合，并进一步保持在温度为50〜100℃，湿度为60〜100％的气氛下，粘附反应层25的平均厚度为50〜1000nm，粘合反应层25与橡胶的界面S的分形维数为1.001〜1.300。

10. 发明授权

US4882987A Attaching device in a spring-charged dot printer 失效
标题翻译：将设备连接到弹簧充电点打印机中
公开(公告)号：US4882987A
公开(公告)日：1989-11-28
申请号：US180635
申请日：1988-04-06
申请人： Yasuo Sakai , Morihito Wada
发明人： Yasuo Sakai , Morihito Wada
IPC分类号： B41J9/10 , B41J9/36 , B41J3/10
CPC分类号： B41J9/36 , B41J9/10
摘要： A retaining plate for use in a spring-charged dot impact printing head has a leaf spring confronting surface which mates with the inclined surface of a support, so that mounting members which fix the leaf springs to the support are substantially parallel to the bottom of the support. Also, in order to uniformly distribute pressure to the leaf springs the confronting surface of the retaining plate is curved inwardly.
摘要翻译：用于弹簧充电的点冲击打印头的保持板具有与支撑件的倾斜表面配合的板簧相对表面，使得将板簧固定到支撑件的安装构件基本上平行于支撑件的底部支持。此外，为了均匀地向板簧分配压力，保持板的相对表面向内弯曲。

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