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    • 3. 发明申请
    • PROTEASE, DNA ENCODING THE SAME, AND METHOD FOR MANUFACTURING PROTEASE
    • PROTEASE,编码其的DNA,以及制造蛋白质的方法
    • US20100015664A1
    • 2010-01-21
    • US12537259
    • 2009-08-07
    • Yoshitaka KANAYAMAYasuo SakaiTakahiro Hongo
    • Yoshitaka KANAYAMAYasuo SakaiTakahiro Hongo
    • C12P21/02C12N15/11C12N1/20C12N1/21
    • C12N9/52
    • A protease having the following characteristics: (i) a molecular weight of 21,000±1,000 (by SDS-PAGE) and (ii) specific protease activity cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (1) below, cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (2) below, or cutting the Ser-Leu site of a peptide or protein comprising amino acid sequence (3) below: Pro-Gln-Gly-Phe-Gln-Gly-Pro(1) (SEQ ID NO: 4) Pro-Ala-Gly-Phe-Ala-Gly-Pro(2) (SEQ ID NO: 5) Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro.(3) (SEQ ID NO: 6) DNA encoding this protease, including vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.
    • 具有以下特征的蛋白酶:(i)分子量为21,000±1,000(通过SDS-PAGE)和(ii)切割包含以下氨基酸序列(1)的肽或蛋白质的Gly-Phe位点的特异性蛋白酶活性, 切割包含以下氨基酸序列(2)的肽或蛋白质的Gly-Phe位点,或切割包含以下氨基酸序列(3)的肽或蛋白质的Ser-Leu位点:Pro-Gln-Gly-Phe-Gln -Gly-Pro(1)(SEQ ID NO:4)Pro-Ala-Gly-Phe-Ala-Gly-Pro(2)(SEQ ID NO:5)Gln-Thr-Gln-Ser-Leu-Val-Thr -Pro。 (3)(SEQ ID NO:6)编码该蛋白酶的DNA,包含含有该DNA的载体,以及通过用该DNA转化细胞产生该蛋白酶的方法。
    • 5. 发明授权
    • Protease, DNA encoding the same, and method for manufacturing protease
    • 蛋白酶,编码相同的DNA,以及制造蛋白酶的方法
    • US07601807B2
    • 2009-10-13
    • US11287307
    • 2005-11-28
    • Yoshitaka KanayamaYasuo SakaiTakahiro Hongo
    • Yoshitaka KanayamaYasuo SakaiTakahiro Hongo
    • C07K1/00
    • C12N9/52
    • A protease having the following characteristics: (i) a molecular weight of 21,000±1,000 (by SDS-PAGE) and (ii) specific protease activity cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (1) below, cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (2) below, or cutting the Ser-Leu site of a peptide or protein comprising amino acid sequence (3) below: (1) (SEQ ID NO:4) Pro-Gln-Gly-Phe-Gln-Gly-Pro (2) (SEQ ID NO:5) Pro-Ala-Gly-Phe-Ala-Gly-Pro (3) (SEQ ID NO:6) Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro. DNA encoding this protease, including vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.
    • 具有以下特征的蛋白酶:(i)分子量为21,000±1,000(通过SDS-PAGE)和(ii)切割包含以下氨基酸序列(1)的肽或蛋白质的Gly-Phe位点的特异性蛋白酶活性, 切割包含以下氨基酸序列(2)的肽或蛋白质的Gly-Phe位点,或切割包含以下氨基酸序列(3)的肽或蛋白质的Ser-Leu位点:(1)(SEQ ID NO:4 )Pro-Gln-Gly-Phe-Gln-Gly-Pro(2)(SEQ ID NO:5)Pro-Ala-Gly-Phe-Ala-Gly-Pro(3)(SEQ ID NO:6)Gln-Thr -Gln-Ser-Leu-Val-Thr-Pro。 编码该蛋白酶的DNA,包括含有该DNA的载体,以及通过用该DNA转化细胞来生产该蛋白酶的方法。