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    • 10. 发明授权
    • Method for synthesizing DNA
    • DNA合成方法
    • US08367328B2
    • 2013-02-05
    • US12285866
    • 2008-10-15
    • Kiyozo AsadaTakashi UemoriYoshimi SatoTomoko FujitaKazue MiyakeOsamu TakedaHiroyuki MukaiIkunoshin Kato
    • Kiyozo AsadaTakashi UemoriYoshimi SatoTomoko FujitaKazue MiyakeOsamu TakedaHiroyuki MukaiIkunoshin Kato
    • C12Q1/68C12P19/34C07H21/02C07H21/04
    • C12P19/34C12N9/1252C12N9/96C12Q1/6806Y10S435/975C12Q2521/107
    • A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.
    • 一种DNA合成反应增强剂,其包含选自酸性物质和阳离子配合物中的至少一种; DNA合成方法,其中在DNA合成反应中,通过使用DNA聚合酶在上述增强剂的存在下进行反应; 包含上述增强剂的DNA合成反应组合物; 包含两种以上各自具有3'→5'核酸外切酶活性的DNA聚合酶的DNA合成反应组合物; 一种DNA合成方法,其中在DNA合成反应中使用两种或更多种具有3'→5'核酸外切酶活性的DNA聚合酶; 用于体外DNA合成的试剂盒,其包含两种或更多种各自具有3'→5'核酸外切酶活性的DNA聚合酶; 以及用于体外DNA合成的试剂盒,其中试剂盒包含DNA合成反应增强剂和DNA聚合酶。 根据本发明,与常规DNA合成反应相比,可以以更优异的效果进行DNA合成。