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1. 发明申请

US20100287668A1 ROSE CONTAINING FLAVONE AND DELPHINIDIN, AND METHOD FOR PRODUCTION THEREOF 审中-公开
标题翻译：含有佛罗伦萨和德芬菲的罗辛，及其生产方法
公开(公告)号：US20100287668A1
公开(公告)日：2010-11-11
申请号：US12665051
申请日：2008-06-19
申请人： Yoshikazu Tanaka , Yukihisa Katsumoto , Yuko Fukui , Masako Mizutani , Noriko Nakamura , Junichi Togami
发明人： Yoshikazu Tanaka , Yukihisa Katsumoto , Yuko Fukui , Masako Mizutani , Noriko Nakamura , Junichi Togami
IPC分类号： A01H5/00 , C12N15/82 , C12N5/10
CPC分类号： C12N15/825 , C12N9/0071 , C12N15/8243
摘要： The invention provides a rose characterized by comprising a flavone and delphinidin added by a genetic modification method. The flavone and delphinidin are typically produced by expression of a transferred flavone synthase gene and flavonoid 3′,5′-hydroxylase gene, respectively. The flavone synthase gene is, for example, a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3′,5′-hydroxylase gene is, for example, the pansy (Viola×wittrockiana) flavonoid 3′,5′-hydroxylase gene.
摘要翻译：本发明提供一种玫瑰，其特征在于包含通过遗传修饰方法添加的黄酮和花翠素。黄酮和花翠素通常分别通过转移的黄酮合成酶基因和类黄酮3'，5'-羟化酶基因的表达产生。黄酮合成酶基因是例如玄参科的黄酮合成酶基因，特别地，它可以是玄参科的金盏花的黄酮合成酶基因或玄参科的肾上腺素的黄酮合成酶基因。类黄酮3'，5'-羟化酶基因为例如三色堇（Viola×wittrockiana）黄酮3'，5'-羟化酶基因。

2. 发明申请

US20100287667A1 ROSE CONTAINING FLAVONE, AND METHOD FOR PRODUCTION THEREOF 审中-公开
标题翻译：含有挥发油的罗斯酮及其生产方法
公开(公告)号：US20100287667A1
公开(公告)日：2010-11-11
申请号：US12664980
申请日：2008-06-19
申请人： Yoshikazu Tanaka , Yukihisa Katsumoto , Yuko Fukui , Masako Mizutani , Noriko Nakamura , Junichi Togami
发明人： Yoshikazu Tanaka , Yukihisa Katsumoto , Yuko Fukui , Masako Mizutani , Noriko Nakamura , Junichi Togami
IPC分类号： A01H5/00 , C12N5/10 , A01H1/00
CPC分类号： C12N15/825 , C12N9/0077
摘要： The invention provides a rose characterized by comprising a flavone added by a genetic modification method. The flavone is typically produced by expression of a transferred flavone synthase gene. The flavone synthase gene is, for example, a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae.
摘要翻译：本发明提供一种玫瑰，其特征在于包含通过遗传修饰方法添加的黄酮。黄酮通常通过转移的黄酮合成酶基因的表达产生。黄酮合成酶基因是例如玄参科的黄酮合成酶基因，特别地，它可以是玄参科的金盏花的黄酮合成酶基因或玄参科的肾上腺素的黄酮合成酶基因。

3. 发明申请

US20100281575A1 ROSE CONTAINING FLAVONE AND MALVIDIN, AND METHOD FOR PRODUCTION THEREOF 审中-公开
标题翻译：含有FLAVONE和MALVIDIN的ROSE及其生产方法
公开(公告)号：US20100281575A1
公开(公告)日：2010-11-04
申请号：US12664998
申请日：2008-06-19
申请人： Yoshikazu Tanaka , Yukihisa Katsumoto , Yuko Fukui , Masako Mizutani , Noriko Nakamura , Junichi Togami
发明人： Yoshikazu Tanaka , Yukihisa Katsumoto , Yuko Fukui , Masako Mizutani , Noriko Nakamura , Junichi Togami
IPC分类号： A01H1/00 , A01H5/00
CPC分类号： C12N9/1007 , C12N9/0071 , C12N9/0073 , C12N15/825
摘要： The invention provides a rose characterized by comprising a flavone and malvidin added by a genetic modification method. The flavone and malvidin are typically produced by expression of a transferred flavone synthase gene, pansy flavonoid 3′,5′-hydroxylase gene and anthocyanin methyltransferase gene. The flavone synthase gene is, for example a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3′,5′-hydroxylase gene is, for example, the pansy flavonoid 3′,5′-hydroxylase gene. The anthocyanin methyltransferase gene is, for example, the methyltransferase gene of torenia of the family Scrophulariaceae.
摘要翻译：本发明提供了一种玫瑰，其特征在于包含通过遗传修饰方法添加的黄酮和malvidin。通常通过转移的黄酮合成酶基因，三色堇黄酮3'，5'-羟化酶基因和花色素苷甲基转移酶基因的表达产生黄酮和malvidin。黄酮合成酶基因是例如玄参科的黄酮合成酶基因，特别地它可以是玄参科的金盏花的黄酮合成酶基因，或玄参科的睾酮的黄酮合成酶基因。类黄酮3'，5'-羟化酶基因是例如三色堇黄酮3'，5'-羟化酶基因。花青素甲基转移酶基因是例如玄参科的肾上腺皮质的甲基转移酶基因。

4. 发明申请

US20100143914A1 METHOD FOR DETERMINATION OF PRESENCE OF CROSSING WITH CULTIVATED ROSE IN WILD ROSE 失效
标题翻译：用于确定在野生玫瑰中具有激发的玫瑰的交叉存在的方法
公开(公告)号：US20100143914A1
公开(公告)日：2010-06-10
申请号：US12532246
申请日：2008-03-21
申请人： Noriko Nakamura , Masako Mizutani , Yoshikazu Tanaka
发明人： Noriko Nakamura , Masako Mizutani , Yoshikazu Tanaka
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , C12Q2600/156
摘要： Disclosed is a method for determining whether or not a wild rose of interest is crossed with a cultivated rose. The method comprises the steps of: examining whether or not a KSN gene containing a transposon (an indicator) is contained in the rose of interest; and determining that the rose of interest is crossed with a cultivated rose when the individual has the transposon-containing KSN gene.
摘要翻译：公开了一种用于确定野生玫瑰是否与栽培的玫瑰杂交的方法。该方法包括以下步骤：检查含有转座子（指标）的KSN基因是否包含在感兴趣的玫瑰中; 并且当个体具有含转座子的KSN基因时，确定感兴趣的玫瑰与栽培的玫瑰杂交。

5. 发明申请

US20080009032A1 Method for Producing Yellow Flower by Controlling Flavonoid Synthetic Pathway 失效
标题翻译：通过控制黄酮合成途径生产黄花的方法
公开(公告)号：US20080009032A1
公开(公告)日：2008-01-10
申请号：US10583110
申请日：2004-12-17
申请人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
发明人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
IPC分类号： A01H5/00 , C07H21/04 , C07K14/00 , C12N15/00 , C12N15/87 , C12N5/04 , C12P21/06
CPC分类号： C12N15/825
摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.
摘要翻译：提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

6. 发明授权

US08350125B2 Method for producing yellow flower by controlling flavonoid synthetic pathway 失效
标题翻译：通过控制黄酮合成途径生产黄花的方法
公开(公告)号：US08350125B2
公开(公告)日：2013-01-08
申请号：US12711701
申请日：2010-02-24
申请人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
发明人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00
CPC分类号： C12N15/825
摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.
摘要翻译：提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

7. 发明授权

US08206928B2 Method for determination of presence of crossing with cultivated rose in wild rose 失效
标题翻译：测定与野生玫瑰栽培玫瑰杂交存在的方法
公开(公告)号：US08206928B2
公开(公告)日：2012-06-26
申请号：US12532246
申请日：2008-03-21
申请人： Noriko Nakamura , Masako Mizutani , Yoshikazu Tanaka
发明人： Noriko Nakamura , Masako Mizutani , Yoshikazu Tanaka
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6895 , C12Q2600/156
摘要： Disclosed is a method for determining whether or not a wild rose of interest is crossed with a cultivated rose. The method comprises the steps of: examining whether or not a KSN gene containing a transposon (an indicator) is contained in the rose of interest; and determining that the rose of interest is crossed with a cultivated rose when the individual has the transposon-containing KSN gene.
摘要翻译：公开了一种用于确定野生玫瑰是否与栽培的玫瑰杂交的方法。该方法包括以下步骤：检查含有转座子（指标）的KSN基因是否包含在感兴趣的玫瑰中; 并且当个体具有含转座子的KSN基因时，确定感兴趣的玫瑰与栽培的玫瑰杂交。

8. 发明申请

US20100199384A1 METHOD FOR PRODUCING YELLOW FLOWER BY CONTROLLING FLAVONOID SYNTHETIC PATHWAY 失效
标题翻译：通过控制FLAVONOID合成途径生产黄花的方法
公开(公告)号：US20100199384A1
公开(公告)日：2010-08-05
申请号：US12711701
申请日：2010-02-24
申请人： Yoshikazu TANAKA , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
发明人： Yoshikazu TANAKA , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
IPC分类号： A01H1/00 , C07H21/04 , C12N15/63 , C12N5/10 , A01H5/00 , C12P1/00
CPC分类号： C12N15/825
摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.
摘要翻译：提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

9. 发明授权

US07750209B2 Method for producing yellow flower by controlling flavonoid synthetic pathway 失效
标题翻译：通过控制黄酮合成途径生产黄花的方法
公开(公告)号：US07750209B2
公开(公告)日：2010-07-06
申请号：US10583110
申请日：2004-12-17
申请人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
发明人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
IPC分类号： C12N15/29 , C12N15/82 , C12N5/04 , A01H5/00
CPC分类号： C12N15/825
摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.
摘要翻译：提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

10. 发明授权

US07514597B2 Glycosyltransferase gene 失效
标题翻译：糖基转移酶基因
公开(公告)号：US07514597B2
公开(公告)日：2009-04-07
申请号：US10524842
申请日：2003-08-20
申请人： Noriko Nakamura , Yuko Fukui , Eiichiro Ono , Yoshikazu Tanaka , Hiroaki Okuhara
发明人： Noriko Nakamura , Yuko Fukui , Eiichiro Ono , Yoshikazu Tanaka , Hiroaki Okuhara
IPC分类号： C12N15/29 , C12N15/52 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8243 , C12N9/1048 , C12N15/825
摘要： The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.
摘要翻译：本发明提供了一种酶，其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应，优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶。此外，本发明提供了使用糖基转移酶改变花色的植物。使用与糖基转移酶保守区相对应的探针，从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。此外，每个糖基转移酶基因在大肠杆菌中表达，将葡萄糖转移到查耳酮的位置2'的活性，即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性，证实克隆的基因在2'位编码糖基转移酶。

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