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1. 发明授权

US07514597B2 Glycosyltransferase gene 失效
标题翻译：糖基转移酶基因
公开(公告)号：US07514597B2
公开(公告)日：2009-04-07
申请号：US10524842
申请日：2003-08-20
申请人： Noriko Nakamura , Yuko Fukui , Eiichiro Ono , Yoshikazu Tanaka , Hiroaki Okuhara
发明人： Noriko Nakamura , Yuko Fukui , Eiichiro Ono , Yoshikazu Tanaka , Hiroaki Okuhara
IPC分类号： C12N15/29 , C12N15/52 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8243 , C12N9/1048 , C12N15/825
摘要： The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.
摘要翻译：本发明提供了一种酶，其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应，优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶。此外，本发明提供了使用糖基转移酶改变花色的植物。使用与糖基转移酶保守区相对应的探针，从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。此外，每个糖基转移酶基因在大肠杆菌中表达，将葡萄糖转移到查耳酮的位置2'的活性，即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性，证实克隆的基因在2'位编码糖基转移酶。

2. 发明授权

US08350125B2 Method for producing yellow flower by controlling flavonoid synthetic pathway 失效
标题翻译：通过控制黄酮合成途径生产黄花的方法
公开(公告)号：US08350125B2
公开(公告)日：2013-01-08
申请号：US12711701
申请日：2010-02-24
申请人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
发明人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00
CPC分类号： C12N15/825
摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.
摘要翻译：提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

3. 发明申请

US20100199384A1 METHOD FOR PRODUCING YELLOW FLOWER BY CONTROLLING FLAVONOID SYNTHETIC PATHWAY 失效
标题翻译：通过控制FLAVONOID合成途径生产黄花的方法
公开(公告)号：US20100199384A1
公开(公告)日：2010-08-05
申请号：US12711701
申请日：2010-02-24
申请人： Yoshikazu TANAKA , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
发明人： Yoshikazu TANAKA , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
IPC分类号： A01H1/00 , C07H21/04 , C12N15/63 , C12N5/10 , A01H5/00 , C12P1/00
CPC分类号： C12N15/825
摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.
摘要翻译：提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

4. 发明授权

US07750209B2 Method for producing yellow flower by controlling flavonoid synthetic pathway 失效
标题翻译：通过控制黄酮合成途径生产黄花的方法
公开(公告)号：US07750209B2
公开(公告)日：2010-07-06
申请号：US10583110
申请日：2004-12-17
申请人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
发明人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
IPC分类号： C12N15/29 , C12N15/82 , C12N5/04 , A01H5/00
CPC分类号： C12N15/825
摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.
摘要翻译：提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

5. 发明申请

US20080009032A1 Method for Producing Yellow Flower by Controlling Flavonoid Synthetic Pathway 失效
标题翻译：通过控制黄酮合成途径生产黄花的方法
公开(公告)号：US20080009032A1
公开(公告)日：2008-01-10
申请号：US10583110
申请日：2004-12-17
申请人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
发明人： Yoshikazu Tanaka , Eiichiro Ono , Noriko Nakamura , Masako Mizutani
IPC分类号： A01H5/00 , C07H21/04 , C07K14/00 , C12N15/00 , C12N15/87 , C12N5/04 , C12P21/06
CPC分类号： C12N15/825
摘要： There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4′CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.
摘要翻译：提供了编码例如SEQ ID NO：2或SEQ ID NO：70所列的氨基酸序列的基因。进行4'CGT基因和AS基因在缺乏天然鸢尾碱合成能力的植物中的共表达，以成功地积累a ones并改变花色，使其具有黄色色调。除了两种基因的表达外，宿主植物本身的类黄酮色素合成途径被抑制以获得更明确的黄色的花。

6. 发明授权

US08628938B2 Polynucleotide encoding UDP-glucuronyl transferase 失效
标题翻译：编码UDP-葡糖醛酸转移酶的多核苷酸
公开(公告)号：US08628938B2
公开(公告)日：2014-01-14
申请号：US12678161
申请日：2008-09-29
申请人： Eiichiro Ono , Akio Noguchi , Yuko Fukui , Masako Mizutani
发明人： Eiichiro Ono , Akio Noguchi , Yuko Fukui , Masako Mizutani
IPC分类号： C12P21/06
CPC分类号： C12N9/10 , C12N9/1051 , C12Y204/01017
摘要： The present invention provides a novel UDP-glucuronosyltransferase and a polynucleotide encoding the same, for example, a polynucleotide comprising nucleotides 1 to 1359 of SEQ ID NO: 4, nucleotides 1 to 1365 of SEQ ID NO: 10, nucleotides 1 to 1371 of SEQ ID NO: 12, and nucleotides 1 to 1371 of SEQ ID NO: 22; or a polynucleotide encoding a protein having the amino acid sequence of SEQ ID NOS: 5, 11, 13 or 23. The invention provides a novel UDP-glucuronosyltransferase with a broad substrate specificity.
摘要翻译：本发明提供新的UDP-葡糖醛酸糖基转移酶和编码该多核苷酸的多核苷酸，例如包含SEQ ID NO：4的核苷酸1至1359，SEQ ID NO：10的核苷酸1至1365，SEQ ID NO：10的核苷酸1至1371，SEQ ID NO： SEQ ID NO：12和SEQ ID NO：22的核苷酸1至1371; 或编码具有SEQ ID NO：5,11,13或23的氨基酸序列的蛋白质的多核苷酸。本发明提供了具有广泛底物特异性的新型UDP-葡糖醛酸基转移酶。

7. 发明申请

US20110104360A1 LIGNAN HYDROXYLASE 失效
标题翻译： LIGNAN羟化酶
公开(公告)号：US20110104360A1
公开(公告)日：2011-05-05
申请号：US12745292
申请日：2008-12-17
申请人： Eiichiro Ono , Asako Okada , Yuko Fukui
发明人： Eiichiro Ono , Asako Okada , Yuko Fukui
IPC分类号： C12N15/63 , C12N9/02 , C07H21/04 , C12N5/10 , A01H5/00 , C12P21/02 , C12P1/00 , C12N15/82 , C07D493/04 , C12P17/18 , A23L1/305
CPC分类号： C12P17/181 , A23L33/18 , C07D493/04 , C12N9/0004 , C12N9/0071 , C12N15/8255
摘要： The invention provides an enzyme having a lignan-hydroxylating activity, particularly an enzyme capable of catalyzing the reaction of transferring a hydroxyl group to a lignan, an enzyme capable of catalyzing the hydroxylation of piperitol to 9-hydroxylpiperitol or pinoresinol to 9-hydroxylpinoresinol. The invention provides a polypeptide having a lignan-hydroxylating activity; a polynucleotide encoding the same; a vector or transformant comprising the polynucleotide; a method for producing a polypeptide having a lignan-hydroxylating activity which comprises using the transformant; and so on. The transformant wherein the polynucleotide is expressibly introduced is useful for the hydroxylation of a lignan or for the production of a product using the same in the food sector and a variety of industry sectors.
摘要翻译：本发明提供了具有木酚素羟化活性的酶，特别是能够催化将羟基转移到木脂素的反应的酶，能够催化胡椒醇羟基化为9-羟基哌啶醇或者尖晶石酚至9-羟基苯甲醇的酶。本发明提供了具有木酚素羟化活性的多肽; 编码该多核苷酸的多核苷酸; 包含所述多核苷酸的载体或转化体; 一种生产具有木酚素羟化活性的多肽的方法，其包括使用所述转化体; 等等。其中可表达引入的多核苷酸的转化体可用于木质素的羟基化或用于在食品部门和各种工业部门中使用其的产品的生产。

8. 发明申请

US20100323402A1 UDP-GLUCURONYL TRANSFERASE AND POLYNUCLEOTIDE ENCODING THE SAME 失效
标题翻译： UDP-GLUCURONYL转移和编码它们的多核苷酸
公开(公告)号：US20100323402A1
公开(公告)日：2010-12-23
申请号：US12678161
申请日：2008-09-29
申请人： Eiichiro Ono , Akio Noguchi , Yuko Fukui , Masako Mizutani
发明人： Eiichiro Ono , Akio Noguchi , Yuko Fukui , Masako Mizutani
IPC分类号： C12P19/60 , C07H21/04 , C07H21/00 , C12N9/10 , C12N15/63 , C12N1/21
CPC分类号： C12N9/10 , C12N9/1051 , C12Y204/01017
摘要： The present invention provides a novel UDP-glucuronosyltransferase and a polynucleotide encoding the same (for example, a polynucleotide comprising a polynucleotide consisting of one nucleotide sequence selected from the group consisting of the nucleotide sequence at positions 1 to 1359 in the nucleotide sequence represented by SEQ ID NO: 4, the nucleotide sequence at positions 1 to 1365 in the nucleotide sequence represented by SEQ ID NO: 10, the nucleotide sequence at positions 1 to 1371 in the nucleotide sequence represented by SEQ ID NO: 12, and the nucleotide sequence at positions 1 to 1371 in the nucleotide sequence represented by SEQ ID NO: 22; or a polynucleotide comprising a polynucleotide encoding a protein having one amino acid sequence selected from the group consisting of SEQ ID NOS: 5, 11, 13 and 23), etc. This provides a novel UDP-glucuronosyltransferase with a broad substrate specificity.
摘要翻译：本发明提供新的UDP-葡糖醛酸糖基转移酶及其编码的多核苷酸（例如，包含多核苷酸的多核苷酸，所述多核苷酸由选自SEQ ID NO：1所示的核苷酸序列中的1〜1359位的核苷酸序列的一个核苷酸序列组成 SEQ ID NO：10所示核苷酸序列中的1〜1365位核苷酸序列，SEQ ID NO：12所示核苷酸序列中1〜1371位核苷酸序列，或包含编码具有选自SEQ ID NO：5,11,13和23的一个氨基酸序列的蛋白质的多核苷酸的多核苷酸等），等等。这提供了具有广泛底物特异性的新型UDP-葡糖醛酸基转移酶。

9. 发明授权

US08288613B2 Lignan hydroxylase 失效
标题翻译：木质素羟化酶
公开(公告)号：US08288613B2
公开(公告)日：2012-10-16
申请号：US12745292
申请日：2008-12-17
申请人： Eiichiro Ono , Asako Okada , Yuko Fukui
发明人： Eiichiro Ono , Asako Okada , Yuko Fukui
IPC分类号： C12N15/82 , C12N15/52 , C12N15/63 , C12N9/00 , C12N15/29 , C12P19/04 , C12P21/02
CPC分类号： C12P17/181 , A23L33/18 , C07D493/04 , C12N9/0004 , C12N9/0071 , C12N15/8255
摘要： The invention provides an enzyme having a lignan-hydroxylating activity, particularly an enzyme capable of catalyzing the reaction of transferring a hydroxyl group to a lignan, an enzyme capable of catalyzing the hydroxylation of piperitol to 9-hydroxylpiperitol or pinoresinol to 9-hydroxylpinoresinol. The invention provides a polypeptide having a lignan-hydroxylating activity; a polynucleotide encoding the same; a vector or transformant comprising the polynucleotide; a method for producing a polypeptide having a lignan-hydroxylating activity which comprises using the transformant; and so on. The transformant wherein the polynucleotide is expressibly introduced is useful for the hydroxylation of a lignan or for the production of a product using the same in the food sector and a variety of industry sectors.
摘要翻译：本发明提供了具有木酚素羟化活性的酶，特别是能够催化将羟基转移到木脂素的反应的酶，能够催化胡椒醇羟基化为9-羟基哌啶醇或者尖晶石酚到9-羟基苯甲醇的酶。本发明提供了具有木酚素羟化活性的多肽; 编码该多核苷酸的多核苷酸; 包含所述多核苷酸的载体或转化体; 一种生产具有木酚素羟化活性的多肽的方法，其包括使用所述转化体; 等等。其中可表达引入的多核苷酸的转化体可用于木质素的羟基化或用于在食品部门和各种工业部门中使用其的产品的生产。

10. 发明申请

US20110219476A1 GLUCURONYL TRANSFERASE AND POLYNUCLEOTIDE ENCODING THE SAME 审中-公开
标题翻译：葡萄糖转移酶和编码它们的多核苷酸
公开(公告)号：US20110219476A1
公开(公告)日：2011-09-08
申请号：US13062093
申请日：2008-09-04
申请人： Eiichiro Ono , Yuko Fukui
发明人： Eiichiro Ono , Yuko Fukui
IPC分类号： A01H5/00 , C12N15/63 , C12N5/10 , C12N1/00 , C12P19/44 , C07H21/04 , C12N9/10
CPC分类号： C12N15/825 , C12N9/1051
摘要： The present invention provides a novel glucuronosyltransferase, a polynucleotide encoding the same (e.g., a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence at positions 1 to 1362 in the nucleotide sequence represented by SEQ ID NO: 7, or a polynucleotide comprising a polynucleotide encoding a protein having the amino acid sequence represented by SEQ ID NO: 8); and so on. A novel glucuronosyltransferase having a broad substrate specificity and others can thus be provided.
摘要翻译：本发明提供了一种新的葡糖醛酸基转移酶，其编码该多核苷酸的多核苷酸（例如，包含由SEQ ID NO：7表示的核苷酸序列中的第1至1362位的核苷酸序列组成的多核苷酸的多核苷酸，或包含编码具有SEQ ID NO：8所示的氨基酸序列的蛋白质）; 等等。因此可以提供具有广泛底物特异性的其它新颖的葡糖醛酸基转移酶等。

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