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    • 6. 发明申请
    • Systems and methods for identifying miRNA targets and for altering miRNA and target expression
    • 用于鉴定miRNA靶标和改变miRNA和靶标表达的系统和方法
    • US20060185027A1
    • 2006-08-17
    • US11317660
    • 2005-12-23
    • David BartelBenjamin LewisMatthew Jones-RhoadesChristopher Burge
    • David BartelBenjamin LewisMatthew Jones-RhoadesChristopher Burge
    • A01K67/027A61K48/00G06F19/00C12N5/08C07H21/02
    • C12N15/111C12N2310/14C12N2320/11
    • The present invention generally relates to microRNAs such as vertebrate microRNA (miRNA), for example, mammalian miRNA. Various aspects of the invention are directed to the detection, production, or expression of miRNA. In one aspect, the invention provides systems and methods for identifying targets of miRNA sequences. For instance, in one embodiment, gene sequences comprising UTRs are compared with miRNA sequences to determine the degree of interaction, for example, by determining a free energy measurement between the miRNA sequence and the UTR, and/or by determining complementarity between at least a portion of the miRNA sequence and the UTR. In another aspect, the invention is directed to the regulation of gene expression using miRNA. For example, gene expression within a cell may be altered by exposing the cell to an oligonucleotide comprising a sequence that is substantially antisense to at least a portion of an miRNA region of the gene, for example, antisense to a 6-mer or 7-mer portion of the miRNA. In still another aspect, the invention is directed to the treatment of cancer. For instance, in one set of embodiments, an isolated oligonucleotide comprising a sequence that is substantially antisense to an miRNA, or a portion of an miRNA, is administered to a subject having or being at risk of cancer. Yet other aspects of the invention are directed to compositions or kits including oligonucleotides comprising a sequence that is substantially antisense to an miRNA (or a portion of an miRNA), methods of promoting any of the above aspects, or the like.
    • 本发明通常涉及微小RNA,例如脊椎动物微小RNA(miRNA),例如哺乳动物miRNA。 本发明的各个方面涉及miRNA的检测,产生或表达。 一方面,本发明提供用于鉴定miRNA序列靶标的系统和方法。 例如,在一个实施方案中,将包含UTR的基因序列与miRNA序列进行比较以确定相互作用程度,例如通过确定miRNA序列和UTR之间的自由能测量,和/或通过确定至少一个 部分miRNA序列和UTR。 另一方面,本发明涉及使用miRNA调节基因表达。 例如,可以通过将细胞暴露于寡核苷酸来改变细胞内的基因表达,所述寡核苷酸包含基本上与基因的miRNA区的至少一部分反义的序列,例如与6-聚体或7- miRNA的部分。 另一方面,本发明涉及癌症的治疗。 例如,在一组实施方案中,将包含基本上与miRNA或一部分miRNA基本反义的序列的分离的寡核苷酸施用于具有或具有癌症风险的受试者。 本发明的其它方面涉及包括寡核苷酸的组合物或试剂盒,其包含基本上与miRNA(或miRNA的一部分)反义的序列,促进任何上述方面的方法等。
    • 7. 发明申请
    • METHOD FOR RECOMMENDING A GIFT TO A SENDER
    • 将礼物推荐给发送者的方法
    • US20130332308A1
    • 2013-12-12
    • US13684089
    • 2012-11-21
    • Lee LindenBenjamin Lewis
    • Lee LindenBenjamin Lewis
    • G06Q30/06G06Q50/00
    • G06Q30/0631G06Q50/01
    • One variation of the method includes: receiving a set of communications from multiple users, the set of communications directed to a recipient through a social networking system; identifying a gift-appropriate event of the recipient based on the set of communications; determining an intent of a sender to respond to the gift-appropriate event of the recipient based on an incomplete electronic note drafted by the sender, within the social networking system, and designating the recipient; selecting a gift for the recipient in response to the determined intent of the sender; and prior to transmission of the note to the recipient, displaying a recommendation to the sender, the recommendation including a suggestion to purchase the gift for the recipient.
    • 该方法的一个变型包括:从多个用户接收一组通信,该通信集合通过社交网络系统指向接收者; 基于所述通信集合来识别所述接收者的礼物适当事件; 基于在所述社交网络系统内由所述发送者起草的不完整的电子笔记以及指定所述接收者来确定发送者对所述接收者的适合礼物的事件的意图; 响应于发送者的确定意图为收件人选择礼物; 并且在向所述收件人发送所述笔记之前,向所述发送者显示推荐,所述建议包括向所述收件人购买礼物的建议。
    • 8. 发明申请
    • METHOD FOR ENABLING A GIFT TRANSACTION
    • 启用礼品交易的方法
    • US20130211943A1
    • 2013-08-15
    • US13615328
    • 2012-09-13
    • Lee LindenBenjamin Lewis
    • Lee LindenBenjamin Lewis
    • G06Q30/06
    • G06Q30/0601G06Q30/0621G06Q30/0633
    • A method of an embodiment includes: receiving a gift request from each of a plurality of senders, each gift request specifying a recipient and a selected gift item; requesting approval of each gift item from a respective recipient; posting a notification of each gift item for publication by an online social network in response to approval of each gift item by a respective recipient; initiating payment for each gift item, by a respective sender, in response to approval of each gift item by a respective recipient; tracking approval and modification of each gift item by a respective recipient; and generating a trend report of gift customization based upon a characteristic of recipients who modify and accept gift items specified by respective senders.
    • 实施例的方法包括:从多个发送者中的每一个接收礼物请求,每个礼物请求指定接收者和所选择的礼物; 要求从相应的接收方批准每个礼品; 在线社交网络发布每个礼品的通知,以响应相应接收者对每个礼品的批准; 响应于相应接收者对每个礼品的批准,由相应的发送者发起每个礼品的支付; 跟踪各个收件人对每个礼物的批准和修改; 并基于修改和接受各个发件人指定的礼物的收件人的特征,生成礼物定制的趋势报告。