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1. 发明申请

US20060233665A1 Test chip and test chip system 审中-公开
标题翻译：测试芯片和测试芯片系统
公开(公告)号：US20060233665A1
公开(公告)日：2006-10-19
申请号：US11327481
申请日：2006-01-09
申请人： Yasuhiko Sasaki , Osamu Kogi , Hiroshi Kishida , Toru Inaba , Masaomi Uchida
发明人： Yasuhiko Sasaki , Osamu Kogi , Hiroshi Kishida , Toru Inaba , Masaomi Uchida
IPC分类号： B01L3/00
CPC分类号： B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2200/143 , B01L2300/0654 , B01L2300/0816 , B01L2300/0867 , B01L2400/0487
摘要： A test chip, which allows a number of solution delivery steps much faster and accurate is provided. The test chip incorporates a sample flow path for containing a sample solution, a reaction flow path for inducing a predetermined reaction with the sample solution, a waste drain path for receiving the used sample, and the washing solution flow paths for containing washing solutions. The reaction flow path contains a plurality of beads having probes of mutually different types fixed thereon. The sample flow path, washing solution flow paths, and sample waste drain path have their respective solution detector units. The solution detector unit detects whether the solution is fed to the path. The detector units adjoiningly provided in the adjacent paths are arranged collinearly.
摘要翻译：提供了一种测试芯片，其允许许多解决方案传送步骤更快速和准确。测试芯片包括用于容纳样品溶液的样品流动路径，用于诱导与样品溶液的预定反应的反应流动路径，用于接收使用的样品的废液排放路径和用于容纳洗涤溶液的洗涤液流路。反应流路包含多个具有固定在其上的相互不同类型的探针的珠。样品流动路径，洗涤液流动路径和样品废物排放路径具有它们各自的溶液检测器单元。溶液检测器单元检测溶液是否被供给到路径。邻接设置在相邻路径中的检测器单元共线布置。

2. 发明申请

US20070243522A1 INSPECTION CHIP FOR BIOLOGICAL MATERIAL 审中-公开
标题翻译：生物材料检验芯片
公开(公告)号：US20070243522A1
公开(公告)日：2007-10-18
申请号：US11679214
申请日：2007-02-27
申请人： Yasuhiko Sasaki , Toru Inaba , Hiroshi Kishida , Osamu Kogi , Masaomi Uchida
发明人： Yasuhiko Sasaki , Toru Inaba , Hiroshi Kishida , Osamu Kogi , Masaomi Uchida
IPC分类号： G01N33/00 , C12M1/00
CPC分类号： B01L3/50273 , B01L2200/027 , B01L2300/0636 , B01L2300/0825 , B01L2300/0877 , B01L2300/14 , B01L2400/0487
摘要： There is provided an inspection chip capable of performing a number of solution feeding processes promptly and accurately. The inspection chip has one continuous flow path comprising a reaction flow path for accommodating a plurality of beads with immobilized probes of types different each other, a first and second solution holding flow path for holding a plurality of solutions each separated by an air gap. The solution is moved from one solution holding flow path to other solution holding flow path via a reaction flow path by utilizing pressure difference.
摘要翻译：提供了能够及时且准确地进行多个溶液供给处理的检查芯片。检查芯片具有一个连续的流动路径，其包括用于容纳具有彼此不同类型的固定化探针的多个珠粒的反应流动路径，用于保持由气隙隔开的多个溶液的第一和第二溶液保持流动路径。通过利用压差将解决方案通过反应流动路径从一个溶液保持流动路径移动到另一溶液保持流动路径。

3. 发明申请

US20080312104A1 BIOLOGICAL MATERIAL PREPARATION CHIP AND PREPARATION CHIP SYSTEM 审中-公开
标题翻译：生物材料制备芯片和制备芯片系统
公开(公告)号：US20080312104A1
公开(公告)日：2008-12-18
申请号：US11763471
申请日：2007-06-15
申请人： Yasuhiko Sasaki , Toru Inaba , Hiroshi Kishida , Masaomi Uchida
发明人： Yasuhiko Sasaki , Toru Inaba , Hiroshi Kishida , Masaomi Uchida
IPC分类号： C40B50/00 , C40B60/14
CPC分类号： B01L3/502746 , B01L2200/0621 , B01L2300/0816 , B01L2300/0867 , B01L2300/0874 , B01L2400/0487 , B01L2400/084
摘要： The present invention is directed to provide a preparation chip system having a simpler configuration and improved reliability while addressing the case where a plurality of reagents have to be fed as in a preparation of extracting DNA from a sample solution. The preparation chip system includes a sample chamber, a dissolving solution chamber, a cleaning solution chamber, an eluting solution chamber, a mixing passage connected to the sample chamber and the dissolving solution chamber and mixing the sample and the dissolving solution with each other, a carrier part connected to the mixing passage, a waste chamber connected to the carrier part via a holding passage, a collection chamber holding the eluting solution passed through the carrier part, and a plurality of resistive materials forming a passage resistor disposed in a first passage connecting the cleaning solution chamber with both the mixing passage and the carrier part. The eluting solution is passed through the carrier part by pressure from a pressure source.
摘要翻译：本发明旨在提供一种制备芯片系统，其具有更简单的结构和改善的可靠性，同时解决了在从样品溶液中提取DNA的制备中必须进料多种试剂的情况。制剂芯片系统包括样品室，溶解溶液室，清洗溶液室，洗脱溶液室，连接到样品室的混合通道和溶解溶液室，并将样品和溶解溶液彼此混合，连接到混合通道的载体部分，经由保持通道连接到载体部分的废物室，保持通过载体部分的洗脱溶液的收集室，以及形成通道电阻器的多个电阻材料，设置在第一通道中，连接所述清洁溶液室具有所述混合通道和所述载体部分。洗脱溶液通过压力源通过载体部分。

4. 发明申请

US20060275891A1 Apparatus and method for reading fluorescence from bead arrays 失效
标题翻译：从珠阵列中读取荧光的装置和方法
公开(公告)号：US20060275891A1
公开(公告)日：2006-12-07
申请号：US11440082
申请日：2006-05-25
申请人： Hiroshi Kishida , Masaomi Uchida
发明人： Hiroshi Kishida , Masaomi Uchida
IPC分类号： C12M1/34 , G01J3/42
CPC分类号： G01N21/6458 , G01N21/55 , G01N21/6452 , G01N2021/174 , G01N2021/6439 , G01N2021/646 , G01N2021/6471 , G01N2021/6478
摘要： When detecting fluorescence of a bead chip array, reflected light from a bead is detected at the same time, so as to recognize the bead position. The reflected light can be detected in a similar manner for all beads, regardless of the presence or absence of a fluorescent substance. If the positions of all beads are detected, accurate detection can be achieved by quantifying only the fluorescence at the detected positions. The fluorescence wavelength alone is detected by a first detector using a wavelength selection filter. Other wavelengths are detected by a second detector, thereby obtaining the reflected light. Data on the reflected light is processed into an image for obtaining the bead profile, the bead position is recognized by detecting the center position based on the profile, and the fluorescence is quantified based on the bead position.
摘要翻译：当检测珠芯片阵列的荧光时，同时检测来自珠粒的反射光，以便识别珠粒位置。不管荧光物质的存在或不存在，都可以以类似的方式检测所有珠子的反射光。如果检测到所有珠粒的位置，则可以通过仅在所检测的位置定量荧光来实现准确的检测。仅使用波长选择滤光片的第一检测器检测荧光波长。其他波长由第二检测器检测，从而获得反射光。将反射光的数据处理成用于获得珠粒轮廓的图像，通过基于轮廓检测中心位置来识别珠粒位置，并且基于珠粒位置来量化荧光。

5. 发明授权

US07732217B2 Apparatus and method for reading fluorescence from bead arrays 失效
标题翻译：从珠阵列中读取荧光的装置和方法
公开(公告)号：US07732217B2
公开(公告)日：2010-06-08
申请号：US11440082
申请日：2006-05-25
申请人： Hiroshi Kishida , Masaomi Uchida
发明人： Hiroshi Kishida , Masaomi Uchida
IPC分类号： G01N21/76 , G01B9/00 , G01J1/00 , G01J3/427 , G02B26/08 , G02B26/10 , G02B26/12 , G06K9/00
CPC分类号： G01N21/6458 , G01N21/55 , G01N21/6452 , G01N2021/174 , G01N2021/6439 , G01N2021/646 , G01N2021/6471 , G01N2021/6478
摘要： When detecting fluorescence of a bead chip array, reflected light from a bead is detected at the same time, so as to recognize the bead position. The reflected light can be detected in a similar manner for all beads, regardless of the presence or absence of a fluorescent substance. If the positions of all beads are detected, accurate detection can be achieved by quantifying only the fluorescence at the detected positions. The fluorescence wavelength alone is detected by a first detector using a wavelength selection filter. Other wavelengths are detected by a second detector, thereby obtaining the reflected light. Data on the reflected light is processed into an image for obtaining the bead profile, the bead position is recognized by detecting the center position based on the profile, and the fluorescence is quantified based on the bead position.
摘要翻译：当检测珠芯片阵列的荧光时，同时检测来自珠粒的反射光，以便识别珠粒位置。不管荧光物质的存在或不存在，都可以以类似的方式检测所有珠子的反射光。如果检测到所有珠粒的位置，则可以通过仅在所检测的位置定量荧光来实现准确的检测。仅使用波长选择滤光片的第一检测器检测荧光波长。其他波长由第二检测器检测，从而获得反射光。将反射光的数据处理成用于获得珠粒轮廓的图像，通过基于轮廓检测中心位置来识别珠粒位置，并且基于珠粒位置来量化荧光。

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