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1. 发明授权

US08709716B2 Analysis of methylation using nucleic acid arrays 有权
标题翻译：使用核酸阵列分析甲基化
公开(公告)号：US08709716B2
公开(公告)日：2014-04-29
申请号：US13015370
申请日：2011-01-27
申请人： Yanxiang Cao , Shivani Nautiyal , Charles G. Miyada , Christopher Davies , Gangwu Mei , Alan J Williams , Eric B. Schell , John E. Blume
发明人： Yanxiang Cao , Shivani Nautiyal , Charles G. Miyada , Christopher Davies , Gangwu Mei , Alan J Williams , Eric B. Schell , John E. Blume
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6883 , C12Q1/6837 , C12Q2600/154 , C12Q2523/125
摘要： Arrays for genome-wide analysis of methylation are disclosed. IN a preferred aspect arrays comprising a plurality of probes complementary to a plurality of identified CpG islands in the human, mouse and rat genome are disclosed. The arrays may be used to detect methylation within cpG islands in samples from human, mouse and rat genomes.
摘要翻译：公开了用于甲基化的全基因组分析的阵列。在优选的方面，公开了包含与人，小鼠和大鼠基因组中的多个鉴定的CpG岛互补的多个探针的阵列。阵列可用于检测来自人，小鼠和大鼠基因组的样品中cpG岛内的甲基化。

2. 发明申请

US20110166037A1 Analysis of methylation using nucleic acid arrays 有权
标题翻译：使用核酸阵列分析甲基化
公开(公告)号：US20110166037A1
公开(公告)日：2011-07-07
申请号：US13015370
申请日：2011-01-27
申请人： Yanxiang Cao , Shivani Nautiyal , Charles G. Miyada , Christopher Davies , Gangwu Mei , Alan J Williams , Eric B. Schell , John E. Blume
发明人： Yanxiang Cao , Shivani Nautiyal , Charles G. Miyada , Christopher Davies , Gangwu Mei , Alan J Williams , Eric B. Schell , John E. Blume
IPC分类号： C40B30/04
CPC分类号： C12Q1/6883 , C12Q1/6837 , C12Q2600/154 , C12Q2523/125
摘要： Arrays for genome-wide analysis of methylation are disclosed. IN a preferred aspect arrays comprising a plurality of probes complementary to a plurality of identified CpG islands in the human, mouse and rat genome are disclosed. The arrays may be used to detect methylation within cpG islands in samples from human, mouse and rat genomes.
摘要翻译：公开了用于甲基化的全基因组分析的阵列。在优选的方面，公开了包含与人，小鼠和大鼠基因组中的多个鉴定的CpG岛互补的多个探针的阵列。阵列可用于检测来自人，小鼠和大鼠基因组的样品中cpG岛内的甲基化。

3. 发明授权

US07901882B2 Analysis of methylation using nucleic acid arrays 有权
标题翻译：使用核酸阵列分析甲基化
公开(公告)号：US07901882B2
公开(公告)日：2011-03-08
申请号：US11695599
申请日：2007-04-02
申请人： Yanxiang Cao , Shivani Nautiyal , Charles G. Miyada , Christopher Davies , Gangwu Mei , Alan J. Williams , Eric B. Schell , John E. Blume
发明人： Yanxiang Cao , Shivani Nautiyal , Charles G. Miyada , Christopher Davies , Gangwu Mei , Alan J. Williams , Eric B. Schell , John E. Blume
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6883 , C12Q1/6837 , C12Q2600/154 , C12Q2523/125
摘要： Arrays for genome-wide analysis of methylation are disclosed. In a preferred aspect arrays comprising a plurality of probes complementary to a plurality of identified CpG islands in the human, mouse and rat genome are disclosed. The arrays may be used to detect methylation within CpG islands in samples from human, mouse and rat genomes.
摘要翻译：公开了用于甲基化的全基因组分析的阵列。在优选的方面，公开了包含与人，小鼠和大鼠基因组中的多个鉴定的CpG岛互补的多个探针的阵列。阵列可用于检测来自人，小鼠和大鼠基因组的样品中CpG岛内的甲基化。

4. 发明授权

US06468744B1 Analysis of genetic polymorphisms and gene copy number 有权
标题翻译：遗传多态性与基因拷贝数分析
公开(公告)号：US06468744B1
公开(公告)日：2002-10-22
申请号：US09341399
申请日：1999-11-17
申请人： Maureen T. Cronin , Edward L. Sheldon , Charles G. Miyada , Earl A. Hubbell , Mark Chee , Stephen P. A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris
发明人： Maureen T. Cronin , Edward L. Sheldon , Charles G. Miyada , Earl A. Hubbell , Mark Chee , Stephen P. A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris
IPC分类号： C12Q168
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00644 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6827 , C12Q1/6837 , C12Q1/6874 , C12Q1/6876 , C12Q1/6883 , C12Q2600/106 , C12Q2600/156 , C40B40/06 , C40B60/14 , C12Q2565/513
摘要： The invention provides methods for detecting variations in polymorphic sites and/or variations in gene copy number. The methods are particularly useful for analysis of biotransformation genes, such as cytochromes P450.
摘要翻译：本发明提供了用于检测多态位点变异和/或基因拷贝数变异的方法。该方法对生物转化基因如细胞色素P450的分析特别有用。

5. 发明授权

US06613516B1 Preparation of nucleic acid samples 有权
标题翻译：核酸样品的制备
公开(公告)号：US06613516B1
公开(公告)日：2003-09-02
申请号：US09689937
申请日：2000-10-11
申请人： Fred C. Christians , Duc Do , Thomas Gingeras , Kevin Gunderson , Charles G. Miyada , Carsten Rosenow , Kai Wu , Qing Yang
发明人： Fred C. Christians , Duc Do , Thomas Gingeras , Kevin Gunderson , Charles G. Miyada , Carsten Rosenow , Kai Wu , Qing Yang
IPC分类号： C12Q168
CPC分类号： C12Q1/6806 , C12N15/1006 , C12Q1/6813 , C12Q1/6837 , Y10S977/958 , C12Q2521/319 , C12Q2563/131
摘要： The presently claimed invention provides methods, compositions, and apparatus for studying nucleic acids. Specifically, the present invention provides a novel enrichment and labeling strategy for ribonucleic acids. In one embodiment, the invention provides enriching for a population of interest in a complex population by diminishing the presence of a target sequence. In a further embodiment, the invention can be used to reproducibly label and detect extremely small amounts of nucleic acids.
摘要翻译：本发明提供了用于研究核酸的方法，组合物和装置。具体地说，本发明提供了核糖核酸的新型富集和标记策略。在一个实施方案中，本发明通过减少靶序列的存在来提供对复杂群体中的感兴趣群体的富集。在另一个实施方案中，本发明可用于可重复地标记和检测极少量的核酸。

6. 发明授权

US5451517A D-arabinitol dehydrogenase from Candida tropicalis ATCC 750 or Candida shehatae 失效
标题翻译：来自热带假丝酵母ATCC 750或嗜酸假丝酵母+ D-的D-阿拉伯糖醇脱氢酶
公开(公告)号：US5451517A
公开(公告)日：1995-09-19
申请号：US400417
申请日：1995-03-03
申请人： Charles G. Miyada , Arthur C. Switchenko , Melanie W. Quong , Man-Ying L. Wong
发明人： Charles G. Miyada , Arthur C. Switchenko , Melanie W. Quong , Man-Ying L. Wong
IPC分类号： C12N9/02 , C12N9/04 , C12N15/02 , C12P19/02 , C12P21/08 , C12Q1/04 , C12Q1/32 , C12R1/72 , C12R1/74 , C12R1/91 , G01N33/53 , G01N33/569 , G01N33/573 , G01N33/577 , C12N1/00 , C12N1/16
CPC分类号： C12N9/0006 , C12P19/02 , Y10S435/81 , Y10S435/921 , Y10S435/924
摘要： A D-arabinitol dehydrogenase enzyme is disclosed. The enzyme is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol and is substantially free of other enzymes capable of oxidizing D-mannitol. Also disclosed are methods for determining D-arabinitol. In one embodiment the method comprises the steps of providing in combination (1) a medium suspected of containing D-arabinitol and (2) a D-arabinitol dehydrogenase enzyme and examining the medium for the product of the oxidation of the D-arabinitol. The enzyme utilized is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol. Kits for conducting the present method are also disclosed. The D-arabinitol dehydrogenase in purified form has all of the characteristics of a D-arabinitol dehydrogenase which is obtainable from Candida tropicalis ATCC 750 or Candida shehatae.
摘要翻译：公开了D-阿拉伯糖醇脱氢酶。该酶能够催化D-阿拉伯糖醇的氧化，并且基本上不能催化D-甘露糖醇的氧化，并且基本上不含能够氧化D-甘露糖醇的其它酶。还公开了测定D-阿拉伯糖醇的方法。在一个实施方案中，该方法包括以下步骤：组合（1）怀疑含有D-阿拉伯糖醇的培养基和（2）D-阿拉伯糖醇脱氢酶，并检查培养基中D-阿拉伯糖醇的氧化产物。所使用的酶能够催化D-阿拉伯糖醇的氧化，并且基本上不能催化D-甘露糖醇的氧化。还公开了用于进行本方法的试剂盒。纯化形式的D-阿拉伯糖醇脱氢酶具有可从热带假丝酵母ATCC 750或念珠菌（Candida shehatae）获得的D-阿拉伯糖醇脱氢酶的所有特征。

7. 发明申请

US20130150248A1 Arrays of Nucleic Acid Probes for Analyzing Biotransformation Genes 审中-公开
标题翻译：用于分析生物转化基因的核酸探针阵列
公开(公告)号：US20130150248A1
公开(公告)日：2013-06-13
申请号：US12959359
申请日：2010-12-02
申请人： Maureen T. Cronin , Charles G. Miyada , Earl A. Hubbell , Mark Chee , Stephen P.A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
发明人： Maureen T. Cronin , Charles G. Miyada , Earl A. Hubbell , Mark Chee , Stephen P.A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00644 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6874 , C12Q1/6876 , C12Q2600/106 , C12Q2600/156 , C12Q2600/172 , C40B40/06 , C40B60/14
摘要： The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.
摘要翻译：本发明提供了固定化探针的阵列，以及采用该阵列的方法，用于检测生物转化基因如细胞色素P450中的突变。例如，一个这样的阵列包括四个探针组。第一探针组包含多个探针，每个探针包含与来自生物转化基因的参考序列的亚序列完全互补的至少三个核苷酸的片段，该片段包含与参考文献中的相应核苷酸互补的至少一个询问位置序列。第二，第三和第四探针组各自包括在第一探针组中的每个探针的相应探针。第二，第三和第四探针组中的探针与包含来自第一探针组的相应探针或包含至少一个询问位置的至少三个核苷酸的亚序列的序列相同，除了至少一个询问位置由来自四个探针组的四个相应探针中的每一个中的不同核苷酸占据。

8. 发明授权

US07794943B2 Modified nucleic acid probes 失效
标题翻译：修饰的核酸探针
公开(公告)号：US07794943B2
公开(公告)日：2010-09-14
申请号：US12124061
申请日：2008-05-20
申请人： Glenn H. McGall , Charles G. Miyada , Maureen T. Cronin , Jennifer Dee Tan , Mark S. Chee
发明人： Glenn H. McGall , Charles G. Miyada , Maureen T. Cronin , Jennifer Dee Tan , Mark S. Chee
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02
CPC分类号： C40B80/00 , B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6832 , C40B40/06 , C40B60/14
摘要： Oligonucleotide analogue arrays attached to solid substrates and methods related to the use thereof are provided. The oligonucleotide analogues hybridize to nucleic acids with either higher or lower specificity than corresponding unmodified oligonucleotides. Target nucleic acids which comprise nucleotide analogues are bound to oligonucleotide and oligonucleotide analogue arrays.
摘要翻译：提供了连接到固体底物的寡核苷酸类似物阵列和与使用相关的方法。寡核苷酸类似物与具有比相应的未修饰寡核苷酸更高或更低特异性的核酸杂交。包含核苷酸类似物的靶核酸与寡核苷酸和寡核苷酸类似物阵列结合。

9. 发明授权

US06489114B2 Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby 有权
标题翻译：用于标记核糖核酸的方法和由此获得的标记的RNA片段
公开(公告)号：US06489114B2
公开(公告)日：2002-12-03
申请号：US09737761
申请日：2000-12-18
申请人： Ali Laayoun , Duc Do , Charles G. Miyada
发明人： Ali Laayoun , Duc Do , Charles G. Miyada
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C12Q1/6806 , C12Q2563/131
摘要： A process is provided for labeling with signal amplification a ribonucleic acid (RNA), comprising fragmenting the RNA to form RNA fragments, fixing a first ligand to a terminal phosphate located at least one of the 3′ end and the 5′ end of each of a plurality of the RNA fragments, the terminal phosphate having been released during the fragmentation, and binding a plurality of labeling agents to the first ligand on each of a plurality of the fragments.
摘要翻译：提供了用信号扩增标记核糖核酸（RNA）的方法，其包括将RNA片段化以形成RNA片段，将第一配体固定在位于每个的3'末端和5'末端中的至少一个末端磷酸酯多个RNA片段，末端磷酸酯在片段化期间被释放，并且将多个标记试剂与多个片段中的每一个上的第一配体结合。

10. 发明授权

US5525717A Support-bound nucletide probe for neisseria gonorrhoeae 失效
标题翻译：淋病奈瑟氏球菌支架结合核苷酸探针+ B
公开(公告)号：US5525717A
公开(公告)日：1996-06-11
申请号：US83946
申请日：1993-06-25
申请人： Charles G. Miyada , Teresa L. Born
发明人： Charles G. Miyada , Teresa L. Born
IPC分类号： C07K7/06 , C07K16/12 , C12N15/31 , C12Q1/68 , C07H21/00 , C12P19/34
CPC分类号： C12Q1/689 , Y10S435/822 , Y10S435/871
摘要： A nucleotide sequence characteristic of Neisseria gonorrhoeae is disclosed. The sequence can be the basis for hybridization type, nucleic acid-based, rapid, in vitro diagnostic assays. The unique nature of the sequence makes it possible to clearly discriminate N. gonorrhoeae from other Neisseria species thus eliminating or substantially reducing the number of false positive readings. A 350 base pair N. gonorrhoeae DNA restriction fragment was cloned after subtractive hybridization to Neisseria meningitidis DNA. In further cloning experiments the sequences adjacent to the original 350 base pair fragment were determined. A portion of this sequence was shown to detect 105 of 106 N. gonorrhoeae strains and no other Neisseria species. In addition to use as detection probes, all or portions of the nucleotide sequence can be used as a ligand for the sandwich capture of N. gonorrhoeae sequences and as primers for in vitro amplification of N. gonorrhoeae sequences. The polypeptides encoded by the presently disclosed sequence, including antibodies thereto, are also disclosed as are their uses.
摘要翻译：披露了淋病奈瑟氏球菌特征的核苷酸序列。该序列可以是杂交类型，基于核酸，快速，体外诊断测定的基础。序列的独特性质使得可以清楚地区分淋病奈瑟氏球菌与其他奈瑟氏菌属物种，从而消除或显着减少假阳性读数的数量。在与奈瑟氏球菌脑膜炎奈瑟菌DNA消减杂交后克隆了350碱基对淋病奈瑟氏球菌DNA限制性片段。在进一步克隆实验中，确定与原始350碱基对片段相邻的序列。该序列的一部分显示检测到106个淋病奈瑟氏球菌菌株中的105个，并且没有其他的奈瑟球菌属物种。除了用作检测探针之外，核苷酸序列的全部或部分可以用作奈氏淋病奈瑟氏球菌序列的夹心捕获的配体，并且用作用于体外扩增淋病奈瑟氏球菌序列的引物。本文公开的序列编码的多肽（包括其抗体）也被用于其用途。

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