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1. 发明申请

US20080241862A1 PROPIONYL AND BUTYRYL LYSINE MODIFICATIONS IN PROTEINS 审中-公开
标题翻译：蛋白质中的丙酸和丁基赖氨酸修饰
公开(公告)号：US20080241862A1
公开(公告)日：2008-10-02
申请号：US12021960
申请日：2008-01-29
申请人： YINGMING ZHAO , John Falck , Yue Chen
发明人： YINGMING ZHAO , John Falck , Yue Chen
IPC分类号： G01N33/574 , G01N33/566 , C07K14/00 , C12P13/08 , C07K16/18 , G01N33/53
CPC分类号： C07K16/44 , C07K16/18 , G01N33/6812 , G01N33/6842
摘要： While the identification of acetylated lysine residues on proteins is well-known, the modification of lysine residues through propionylation and butyrylation is not very well understood. A method for the identification and mapping of propionylated and butyrylated lysine residues has been developed. Anti-acetyllysine antibody, normally used to affinity purify a protein mixture based on the presence of acetylated lysine, can also be used to affinity purity proteins having propionylated and butyrylated lysine residues due to the structural similarity. The method involves searching protein databases to locate mass spectrometry datasets for those proteins purified by anti-acetyllysine antibody. The located spectra are manually reviewed to identify those peptides having propionyllysine and butyryllysine residues. These identified peptides are synthesized, with the lysine modifications added at the appropriate positions. The synthesized proteins are then analyzed with mass spectrometry and the resultant spectra are compared to those located in the protein databases to confirm the location of the lysine modifications.
摘要翻译：虽然蛋白质上的乙酰化赖氨酸残基的鉴定是众所周知的，但是通过丙酰化和丁酰化修饰赖氨酸残基不是很好地理解。已经开发了用于鉴定和测定丙酰化和丁酰化赖氨酸残基的方法。通常用于亲和纯化基于乙酰化赖氨酸存在的蛋白质混合物的抗乙酰赖氨酸抗体也可用于由于结构相似性而具有丙酰化和丁酰化赖氨酸残基的亲和纯度蛋白质。该方法包括搜索蛋白质数据库来定位由抗乙酰赖氨酸抗体纯化的蛋白质的质谱数据集。手工检查定位的光谱，以鉴定具有丙酰赖氨酸和丁酰赖氨酸残基的那些肽。合成这些鉴定的肽，其中在适当位置加入赖氨酸修饰。然后通过质谱分析合成的蛋白质，并将所得光谱与位于蛋白质数据库中的蛋白质进行比较，以确认赖氨酸修饰的位置。

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