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1. 发明授权

US06972183B1 Capillary array-based enzyme screening 有权
标题翻译：基于毛细管阵列的酶筛选
公开(公告)号：US06972183B1
公开(公告)日：2005-12-06
申请号：US09444112
申请日：1999-11-22
申请人： William Michael Lafferty , Jay M. Short , Martin Keller
发明人： William Michael Lafferty , Jay M. Short , Martin Keller
IPC分类号： C12N15/10 , G01N33/573
CPC分类号： C12N15/1086
摘要： A process for screening an expression library to identify clones expressing enzymes having a desired activity is provided. The process involves first generating from genomic DNA samples of one or more microorganisms an expression library comprising a plurality of recombinant cell clones, and then introducing into capillaries in a capillary array a substrate and at least a subset of the clones, either individually or as a mixture. Interaction of the substrate and a clone expressing an enzyme having the desired activity produces an optically detectable signal, which can then be spatially detected to identify capillaries containing clones producing such a signal. The signal-producing clones can then be recovered from the identified capillaries.
摘要翻译：提供了筛选表达文库以鉴定表达具有所需活性的酶的克隆的方法。该方法包括首先从一种或多种微生物的基因组DNA样品产生包含多个重组细胞克隆的表达文库，然后将毛细管阵列中的毛细管阵列引入基质和克隆的至少一个子集，单独地或作为混合物。底物和表达具有所需活性的酶的克隆的相互作用产生光学上可检测的信号，然后可以在空间上检测，以鉴定含有产生这种信号的克隆的毛细血管。然后可以从识别的毛细血管中回收信号产生克隆。

2. 发明授权

US06866824B2 Capillary array-based sample screening 有权
标题翻译：基于毛细管阵列的样品筛选
公开(公告)号：US06866824B2
公开(公告)日：2005-03-15
申请号：US10157653
申请日：2002-05-28
申请人： William Michael Lafferty , Jay M. Short , Martin Keller
发明人： William Michael Lafferty , Jay M. Short , Martin Keller
IPC分类号： B01J19/00 , B01L3/00 , C12N15/10 , C12Q1/68 , C40B40/02 , C40B40/06 , C40B60/14 , G01N21/00
CPC分类号： C40B40/02 , B01J19/0046 , B01J2219/00351 , B01J2219/00367 , B01J2219/00416 , B01J2219/00479 , B01J2219/00481 , B01J2219/00522 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00686 , B01J2219/00689 , B01J2219/00702 , B01J2219/00722 , B01J2219/0074 , B01J2219/00743 , B01L3/5085 , B01L3/50857 , B82Y30/00 , C12N15/1037 , C12N15/1055 , C12Q1/6811 , C12Q1/6837 , C40B40/06 , C40B60/14
摘要： Provided are methods of screening and identification of bioactivities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries and recovering bioactive molecules having a desired activity or a nucleic acid sequence encoding such bioactive molecules.
摘要翻译：提供使用毛细管阵列系统筛选和鉴定感兴趣的生物活性分子的方法。更具体地，公开了使用光学检测和毛细管阵列技术来筛选文库和回收具有所需活性的生物活性分子或编码这种生物活性分子的核酸序列的方法。

3. 发明授权

US06794127B1 Capillary array-based sample screening 有权
标题翻译：基于毛细管阵列的样品筛选
公开(公告)号：US06794127B1
公开(公告)日：2004-09-21
申请号：US09687219
申请日：2000-10-12
申请人： William Michael Lafferty , Jay M. Short , Martin Keller
发明人： William Michael Lafferty , Jay M. Short , Martin Keller
IPC分类号： C12Q100
CPC分类号： C40B40/02 , B01J19/0046 , B01J2219/00351 , B01J2219/00367 , B01J2219/00416 , B01J2219/00479 , B01J2219/00481 , B01J2219/00522 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00686 , B01J2219/00689 , B01J2219/00702 , B01J2219/00722 , B01J2219/0074 , B01J2219/00743 , B01L3/5085 , B01L3/50857 , B82Y30/00 , C12N15/1037 , C12N15/1055 , C12Q1/6811 , C12Q1/6837 , C40B40/06 , C40B60/14
摘要： Provided are methods of screening and identification of bio activities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries and recovering bioactive molecules having a desired activity or a nucleic acid sequence encoding such bioactive molecules.
摘要翻译：提供了使用毛细管阵列系统筛选和鉴定生物活性和生物活性分子的方法。更具体地，公开了使用光学检测和毛细管阵列技术来筛选文库和回收具有所需活性的生物活性分子或编码这种生物活性分子的核酸序列的方法。

4. 发明授权

US06872526B2 High throughput screening for novel Bioactivities 有权
标题翻译：高通量筛选新型生物活性
公开(公告)号：US06872526B2
公开(公告)日：2005-03-29
申请号：US09848095
申请日：2001-05-03
申请人： Jay M. Short , Martin Keller
发明人： Jay M. Short , Martin Keller
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68 , C40B40/02
CPC分类号： C40B40/02 , C12N15/1037 , C12N15/1055 , C12Q1/6811
摘要： Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.
摘要翻译：公开了用于鉴定具有特定的感兴趣活性的克隆的方法，该方法包括（i）产生一种或多种源自从环境直接分离的核酸的表达文库; 和（ii）使用荧光激活细胞分选仪筛选所述文库以鉴定所述克隆。更具体地说，这是通过（i）产生一种或多种从环境中直接或间接分离的核酸产生的表达文库来鉴定具有特定活性的克隆的方法; （ii）将所述文库暴露于感兴趣的特定底物或底物; 和（iii）使用荧光激活的细胞分选仪筛选所述暴露的文库，以鉴定与底物或底物反应的克隆。还提供了通过（i）生成从环境直接或间接分离的核酸产生的一种或多种表达文库来鉴定具有特定活性的克隆的方法; 和（ii）使用需要共包封，结合事件或靶共价修饰的测定法和荧光激活的细胞分选器筛选所述暴露的文库以鉴定阳性克隆。

5. 发明授权

US06602675B2 High throughput screening of mycelia for bioactivities or biomolecules 有权
标题翻译：用于生物活性或生物分子的菌丝体的高通量筛选
公开(公告)号：US06602675B2
公开(公告)日：2003-08-05
申请号：US09848083
申请日：2001-05-03
申请人： Jay M. Short , Martin Keller
发明人： Jay M. Short , Martin Keller
IPC分类号： G01N33569
CPC分类号： C40B40/02 , C12N15/1037 , C12N15/1055 , C12Q1/6811
摘要： Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.
摘要翻译：公开了用于鉴定具有特定的感兴趣活性的克隆的方法，该方法包括（i）产生一种或多种源自从环境直接分离的核酸的表达文库; 和（ii）使用荧光激活细胞分选仪筛选所述文库以鉴定所述克隆。更具体地说，这是通过（i）产生一种或多种从环境中直接或间接分离的核酸产生的表达文库来鉴定具有特定活性的克隆的方法; （ii）将所述文库暴露于感兴趣的特定底物或底物; 和（iii）使用荧光激活的细胞分选仪筛选所述暴露的文库，以鉴定与底物或底物反应的克隆。还提供了通过（i）生成从环境直接或间接分离的核酸产生的一种或多种表达文库来鉴定具有特定活性的克隆的方法; 和（ii）使用需要共包封，结合事件或靶共价修饰的测定法和荧光激活的细胞分选器筛选所述暴露的文库以鉴定阳性克隆。

6. 发明授权

US06174673B1 High throughput screening for novel enzymes 失效
公开(公告)号：US06174673B1
公开(公告)日：2001-01-16
申请号：US09098206
申请日：1998-06-16
申请人： Jay M. Short , Martin Keller
发明人： Jay M. Short , Martin Keller
IPC分类号： C12Q168
CPC分类号： C40B40/02 , C12N15/1037 , C12N15/1055 , C12Q1/6811
摘要： Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nuclei acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.

7. 发明授权

US06806048B2 Method for high throughput screening of an environmental library 有权
标题翻译：环境图书馆高通量筛选方法
公开(公告)号：US06806048B2
公开(公告)日：2004-10-19
申请号：US09848651
申请日：2001-05-03
申请人： Jay M. Short , Martin Keller
发明人： Jay M. Short , Martin Keller
IPC分类号： C12Q168
CPC分类号： C40B40/02 , C12N15/1037 , C12N15/1055 , C12Q1/6811
摘要： Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.
摘要翻译：公开了用于鉴定具有特定的感兴趣活性的克隆的方法，该方法包括（i）产生一种或多种源自从环境直接分离的核酸的表达文库; 和（ii）使用荧光激活细胞分选仪筛选所述文库以鉴定所述克隆。更具体地说，这是通过（i）产生一种或多种从环境中直接或间接分离的核酸产生的表达文库来鉴定具有特定活性的克隆的方法; （ii）将所述文库暴露于感兴趣的特定底物或底物; 和（iii）使用荧光激活的细胞分选仪筛选所述暴露的文库，以鉴定与底物或底物反应的克隆。还提供了通过（i）生成从环境直接或间接分离的核酸产生的一个或多个表达文库来鉴定具有特定活性的克隆的方法; 和（ii）使用需要共包封，结合事件或靶共价修饰的测定法和荧光激活的细胞分选器筛选所述暴露的文库以鉴定阳性克隆。

8. 发明申请

US20110109032A1 INSERTION APPARATUS AND INSERTION METHOD 有权
标题翻译：插入装置和插入方法
公开(公告)号：US20110109032A1
公开(公告)日：2011-05-12
申请号：US12997379
申请日：2009-06-09
申请人： Martin Keller
发明人： Martin Keller
IPC分类号： B43M3/00
CPC分类号： B41F13/68 , B65H5/30 , B65H5/305 , B65H29/003 , B65H29/40 , B65H39/065 , B65H2301/431711 , B65H2301/431715 , B65H2301/44712 , B65H2301/44765 , B65H2404/655 , B65H2404/6551 , B65H2404/6582 , B65H2406/12 , B65H2701/1932 , B65H2220/01 , B65H2220/02
摘要： An apparatus for, and a method of, inserting flat articles, e.g. printed pre-products or additional products, parts of newspapers, goods samples, leaflets, fliers and other inserts, into folded printed products. Use is made of a rotary system with compartments which run along a closed circular rotary path and are oriented as a tangent or secant to the rotary path (U). This makes it possible for the printed products and the articles to be fed in the direction of rotation, as a result of which the forces acting on the products can be reduced. There are also advantages relating to format adaptation, since the opening angle of the product upon adjustment of the compartment base does not change to such a pronounced extent as is the case with radially oriented compartments.
摘要翻译：用于插入扁平制品的装置和方法，例如。印刷的预产品或附加产品，报纸，货品样本，传单，传单和其他插页的部件，折叠印刷产品。使用旋转系统，其具有沿着封闭的圆形旋转路径延伸并且被定向为旋转路径（U）的切线或切线的隔间。这使得打印产品和制品可以沿旋转方向进给，结果可以减小作用在产品上的力。还有与格式适配有关的优点，因为在调节隔间基座时产品的开启角度不会如同径向取向的隔间的情况那样明显地变化。

9. 发明申请

US20100089903A1 Cooking Appliance 审中-公开
标题翻译：烹饪用具
公开(公告)号：US20100089903A1
公开(公告)日：2010-04-15
申请号：US11992218
申请日：2006-09-12
申请人： Ingo Bally , Kerstin Feldmann , Wolfgang Fuchs , Martin Keller , Edmund Kuttalek , Maximilian Neuhauser , Klemens Roch , Wolfgang Schnell , Günter Zschau
发明人： Ingo Bally , Kerstin Feldmann , Wolfgang Fuchs , Martin Keller , Edmund Kuttalek , Maximilian Neuhauser , Klemens Roch , Wolfgang Schnell , Günter Zschau
IPC分类号： F24C15/02 , F27D11/00
CPC分类号： F24C15/027
摘要： A cooking appliance, in particular, a cooking appliance which is mounted in an elevated manner, comprising a muffle which defines a cooking chamber and electric and/or electronic components which are arranged inside the door. The electric connection elements are formed by cables, in particular, having strain relief, and at least one part of the cable is received via at least one part of the longitudinal extension thereof in a cable guide, in order to enable a secure cable connection between the electric and/or electronic components in the door and the corresponding connections in the housing, without the cables being guided in an uncontrollable manner in a free chamber of the housing or rub against each other or against additional components inside the housing and as a result become worn.
摘要翻译：一种烹饪器具，特别是以升高的方式安装的烹饪器具，包括限定烹饪室的马弗炉和布置在门内的电气和/或电子部件。电连接元件由电缆形成，特别是具有应变消除，并且电缆的至少一部分经由电缆引导件的纵向延伸部的至少一部分被接收，以便能够在电缆引导件之间进行安全的电缆连接门中的电气和/或电子部件和壳体中的对应连接，而不会使电缆以不可控制的方式被引导到壳体的自由腔室中或彼此摩擦或抵抗壳体内的附加部件变得磨损

10. 发明申请

US20090159585A1 Cooking Appliance which is Mounted in an Elevated Manner 审中-公开
标题翻译：以高架方式安装的烹饪用具
公开(公告)号：US20090159585A1
公开(公告)日：2009-06-25
申请号：US11992221
申请日：2006-09-18
申请人： Ingo Bally , Kerstin Feldmann , Wolfgang Fuchs , Martin Keller , Edmund Kuttalek , Maximilian Neuhauser , Klemens Roch , Wolfgang Schnell , Guenter Zschau
发明人： Ingo Bally , Kerstin Feldmann , Wolfgang Fuchs , Martin Keller , Edmund Kuttalek , Maximilian Neuhauser , Klemens Roch , Wolfgang Schnell , Guenter Zschau
IPC分类号： F27B5/14
CPC分类号： F24C7/087 , F24C7/082 , F24C15/027
摘要： A cooking appliance which is mounted in an elevated manner, comprising at least one muffle which defines a cooking chamber and which comprises a muffle opening on the base side thereof, a base door, which can be displaced, for closing the muffle opening and which comprises at least one heating field on the upper side thereof, having at least one operational state for the open state of the base door and at least one operational state for the closed state of base door. Only one operational state, which is relevant for the opening state of the base door, can be activated, and only one operational state for the open state when the base door is open and only one operational state for the closed state when the base door is closed.
摘要翻译：一种以升高的方式安装的烹饪器具，包括限定烹饪室的至少一个马弗炉，其包括在其基部侧上的马弗管开口，可移动的基座门，用于封闭马弗管孔，并且包括在其上侧具有至少一个加热场，具有至少一个操作状态用于基本门的打开状态和至少一个操作状态，用于关闭门。与基门打开状态相关的只有一个操作状态可以被激活，并且当基门打开时只有一个操作状态用于打开状态，并且当基门是只有一个关闭状态的操作状态关闭。

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