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1. 发明授权

US10670608B2 Comprehensive monoclonal antibody generation 审中-公开
公开(公告)号：US10670608B2
公开(公告)日：2020-06-02
申请号：US13977193
申请日：2011-12-28
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C40B30/04 , G01N33/68 , C07K16/00 , G01N33/50 , G01N33/577
摘要： The present invention relates to methods for efficiently generating recombinant monoclonal antibodies derived from B cells of a non-human host which has been immunochallenged with one or more target antigens. The methods comprise the steps of identifying and isolating B cell that bind to the antigen by FACS, and recombining and enriching for thousands of cells to create a B cell library. Related products and methods, such as methods of producing expression libraries, are also disclosed.

2. 发明授权

US08709755B2 Mirac proteins 有权
标题翻译： Mirac蛋白
公开(公告)号：US08709755B2
公开(公告)日：2014-04-29
申请号：US13523509
申请日：2012-06-14
申请人： Jay M. Short , Hwai Wen Chang , Gerhard Frey
发明人： Jay M. Short , Hwai Wen Chang , Gerhard Frey
IPC分类号： C12P21/06 , C12Q1/68 , C07K1/00 , C12N5/07 , C12N15/00
CPC分类号： C12N15/102 , A61K38/00 , C07K7/06 , C07K14/3153 , C07K14/47 , C07K14/575 , C07K14/57545 , C07K14/57563 , C12N9/16 , C12N9/50 , C12N9/6435 , C12N9/6459 , C12N9/6462 , C12N15/1058 , C12Y302/01035 , C12Y304/21007 , C12Y304/21069 , C12Y304/21073 , C12Y304/23015 , C12Y304/24029 , G01N33/54306 , G01N33/573 , G01N33/6845 , G01N33/6854 , Y02A50/473
摘要： This disclosure relates to a method of generating conditionally active biologic proteins from wild type proteins, in particular therapeutic proteins, which are reversibly or irreversibly inactivated at the wild type normal physiological conditions. For example, evolved proteins are virtually inactive at body temperature, but are active at lower temperatures.
摘要翻译：本公开涉及从野生型正常生理条件下可逆地或不可逆地灭活的野生型蛋白质，特别是治疗性蛋白质产生条件活性生物蛋白质的方法。例如，进化蛋白质在体温实际上是无活性的，但在较低温度下是活性的。

3. 发明授权

US08334118B2 Enzymes having alpha amylase activity and methods of making and using them 有权
标题翻译：具有α-淀粉酶活性的酶和制备和使用它们的方法
公开(公告)号：US08334118B2
公开(公告)日：2012-12-18
申请号：US12567550
申请日：2009-09-25
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Jay M. Short , Eric J. Mathur , Kevin A. Gray , Janne S. Kerovuo , Malgorzata Slupska
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Jay M. Short , Eric J. Mathur , Kevin A. Gray , Janne S. Kerovuo , Malgorzata Slupska
IPC分类号： C12N9/28 , C12P19/14 , C12S9/00 , C12S11/00 , C12S3/00 , C11D3/00
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： The invention relates to alpha amylases and to polynucleotides and polypeptides encoding the alpha amylases. In addition, methods of using the alpha amylases are also provided. The alpha amylases have increased activity and stability at acidic, neutral and alkaline pH and at increased temperatures.
摘要翻译：本发明涉及α-淀粉酶和编码α-淀粉酶的多核苷酸和多肽。此外，还提供了使用α-淀粉酶的方法。在酸性，中性和碱性pH和升高的温度下，α-淀粉酶具有增加的活性和稳定性。

4. 发明申请

US20110165627A1 GENE SITE SATURATION MUTAGENESIS 审中-公开
标题翻译：基因座饱和度
公开(公告)号：US20110165627A1
公开(公告)日：2011-07-07
申请号：US12817616
申请日：2010-06-17
申请人： Jay M. SHORT
发明人： Jay M. SHORT
IPC分类号： C12P19/34
CPC分类号： C07K14/445 , A61K39/00 , A61K2039/53 , C07K16/00 , C07K16/005 , C07K2317/565 , C12N9/00 , C12N9/14 , C12N9/16 , C12N15/102 , C12N15/1027 , C12N15/1034
摘要： A method for producing progeny polynucleotides and polypeptides by Gene Site Saturation Mutagenesis (GSSM). The method provides a set of degenerate primers corresponding to codons of a template polynucleotide, and performs polymerase elongation to produce progeny polynucleotides, which contain sequences corresponding to the degenerate primers. The progeny polynucleotides can be expressed and screened for directed evolution.
摘要翻译：通过基因位点饱和诱变（GSSM）产生后代多核苷酸和多肽的方法。该方法提供了一组对应于模板多核苷酸的密码子的简并引物，并进行聚合酶延伸以产生含有对应于简并引物的序列的后代多核苷酸。可以表达和筛选后代多核苷酸用于定向进化。

5. 发明授权

US07666633B2 Enzymes having alpha amylase activity and methods of making and using them 有权
标题翻译：具有α-淀粉酶活性的酶和制备和使用它们的方法
公开(公告)号：US07666633B2
公开(公告)日：2010-02-23
申请号：US11400030
申请日：2006-04-06
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Jay M. Short , Eric J. Mathur
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Jay M. Short , Eric J. Mathur
IPC分类号： C12P19/14 , C12P7/06 , C12N9/26 , D06M16/00 , C07H21/04 , A21D8/02
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases, and methods of making and using them. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及α-淀粉酶和编码α-淀粉酶的多核苷酸，以及制备和使用它们的方法。另外还提供了设计新的α-淀粉酶的方法及其使用方法。 α淀粉酶在增加的pH和温度下具有增加的活性和稳定性。

6. 发明授权

US07659102B2 Amylases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：淀粉酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US07659102B2
公开(公告)日：2010-02-09
申请号：US10489510
申请日：2003-03-24
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Carl A. Miller , Martin Kazaoka , Eric J. Mathur , Jay M. Short
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Carl A. Miller , Martin Kazaoka , Eric J. Mathur , Jay M. Short
IPC分类号： C12P1/00 , C12N9/00 , C12N9/48 , C12N1/20 , C12N15/00
CPC分类号： C12N9/2411 , C12N9/2417 , C12P19/14
摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for malting and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into 10 sugars.
摘要翻译：一方面，本发明涉及具有淀粉酶活性的多肽，编码多肽的多核苷酸，以及用于发芽和使用这些多核苷酸和多肽的方法。一方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化淀粉水解成10个糖。

7. 发明授权

US07432097B2 Phytases, nucleic acids encoding them and methods of making and using them 有权
标题翻译：植酸酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US07432097B2
公开(公告)日：2008-10-07
申请号：US10933115
申请日：2004-09-01
申请人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , William Baum , Dan E. Robertson , Paul Zorner
发明人： Jay M. Short , Keith A. Kretz , Kevin A. Gray , Nelson Robert Barton , James B. Garrett , Eileen O'Donoghue , William Baum , Dan E. Robertson , Paul Zorner
IPC分类号： C12N11/00 , C12N9/16 , A61K38/46 , C07K14/00 , C12N15/00 , C12N1/21 , C12N5/10 , C12P21/00 , C12Q1/44 , C07H21/00
CPC分类号： C12N9/16 , A01K2217/05 , A21D8/042 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K33/00 , A61K36/00 , A61K38/00 , A61K45/06 , C12Y301/03008 , C12Y301/03026 , Y02P60/877 , A23V2300/21
摘要： In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译：一方面，本发明提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。此外，该酶在低pH下具有改善的蛋白酶稳定性。修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。一方面，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。

8. 发明授权

US07232677B2 Phytase expression systems and methods of making and using them 有权
标题翻译：植酸酶表达系统及其使用方法
公开(公告)号：US07232677B2
公开(公告)日：2007-06-19
申请号：US09777566
申请日：2001-02-05
申请人： Jay M. Short , Keith A. Kretz
发明人： Jay M. Short , Keith A. Kretz
IPC分类号： C07H21/04 , C12N15/00 , C12N5/10 , C12N1/21 , C12N9/14 , C12N9/16 , C12Q1/42 , C12Q1/44
CPC分类号： C12N15/8242 , A01K2217/05 , A21D8/042 , A23K20/189 , A23L5/25 , A23L7/101 , A23L11/00 , A23L11/33 , A23L29/06 , A23L33/17 , A23L33/185 , A61K38/00 , C12C5/004 , C12G2200/15 , C12N9/16 , C12N15/8243 , C12N15/8245 , C12Y301/03026
摘要： The invention provides a purified or recombinant phytase enzyme (SEQ ID NO:2) initially derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译：本发明提供了最初衍生自大肠杆菌B的纯化或重组植酸酶（SEQ ID NO：2）。该酶具有约47.1千道尔顿的分子量并具有植酸酶活性（SEQ ID NO：2）。该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。

9. 发明授权

US07078504B2 Enzymes having dehalogenase activity and methods of use thereof 有权
公开(公告)号：US07078504B2
公开(公告)日：2006-07-18
申请号：US10000997
申请日：2001-11-30
申请人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
发明人： Jay M. Short , Toby Richardson , Dan E. Robertson , Kevin A. Gray
IPC分类号： C07H21/02 , C12Q1/68
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding alkane dehalogenases. In addition methods of designing new dehalogenases and of use thereof are also provided. The dehalogenases have increased activity and at increased pH and temperature.

10. 发明授权

US06972183B1 Capillary array-based enzyme screening 有权
标题翻译：基于毛细管阵列的酶筛选
公开(公告)号：US06972183B1
公开(公告)日：2005-12-06
申请号：US09444112
申请日：1999-11-22
申请人： William Michael Lafferty , Jay M. Short , Martin Keller
发明人： William Michael Lafferty , Jay M. Short , Martin Keller
IPC分类号： C12N15/10 , G01N33/573
CPC分类号： C12N15/1086
摘要： A process for screening an expression library to identify clones expressing enzymes having a desired activity is provided. The process involves first generating from genomic DNA samples of one or more microorganisms an expression library comprising a plurality of recombinant cell clones, and then introducing into capillaries in a capillary array a substrate and at least a subset of the clones, either individually or as a mixture. Interaction of the substrate and a clone expressing an enzyme having the desired activity produces an optically detectable signal, which can then be spatially detected to identify capillaries containing clones producing such a signal. The signal-producing clones can then be recovered from the identified capillaries.
摘要翻译：提供了筛选表达文库以鉴定表达具有所需活性的酶的克隆的方法。该方法包括首先从一种或多种微生物的基因组DNA样品产生包含多个重组细胞克隆的表达文库，然后将毛细管阵列中的毛细管阵列引入基质和克隆的至少一个子集，单独地或作为混合物。底物和表达具有所需活性的酶的克隆的相互作用产生光学上可检测的信号，然后可以在空间上检测，以鉴定含有产生这种信号的克隆的毛细血管。然后可以从识别的毛细血管中回收信号产生克隆。

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