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1. 发明授权

US07741102B2 Analyte detection and apparatus therefor 失效
标题翻译：分析物检测及其设备
公开(公告)号：US07741102B2
公开(公告)日：2010-06-22
申请号：US10257063
申请日：2001-04-09
申请人： William Ewen Smith , Duncan Graham , Callum John McHugh , Ruth Lyndsey Keir , Peter Cyril White , Mairi Campbell
发明人： William Ewen Smith , Duncan Graham , Callum John McHugh , Ruth Lyndsey Keir , Peter Cyril White , Mairi Campbell
IPC分类号： C12M1/00
CPC分类号： C07D213/53 , C07D213/79 , C07D215/38 , C07D249/18 , C07D333/12 , C07D403/04 , C07D487/08 , G01N21/658 , Y10T436/25
摘要： The present invention relates to sensitive SE(R)RS based methods for detecting analytes such as explosives and drugs, which may be present in a sample at extremely low levels. The methods may be generally carried out in situ employing novel chemistry which is compatible with flow-cell technology and with time-scales and concentrations required for rapid and informative screening of large numbers of samples. The present invention also relates to novel compounds e.g. synthons and apparatus for use with the methods disclosed.
摘要翻译：本发明涉及用于检测分析物（例如爆炸物和药物）的敏感的基于SE（R）RS的方法，其可以以极低的水平存在于样品中。这些方法通常可以原位采用与流动池技术相适应的新颖化学方法，并且具有快速和翔实筛选大量样品所需的时间和浓度。本发明还涉及新化合物，例如合成子和与所公开方法一起使用的装置。

2. 发明申请

US20100035359A1 FUNCTIONALISED POLYMERS FOR BINDING METAL SURFACES 有权
标题翻译：用于结合金属表面的功能聚合物
公开(公告)号：US20100035359A1
公开(公告)日：2010-02-11
申请号：US12446817
申请日：2007-10-23
申请人： Peter Cormack , Duncan Graham , Aaron Hernandez-Santana , Arun Prasath Ramaswamy , William Ewen Smith
发明人： Peter Cormack , Duncan Graham , Aaron Hernandez-Santana , Arun Prasath Ramaswamy , William Ewen Smith
IPC分类号： G01N21/62 , C08F8/00 , C08F283/00 , C08G63/91 , C08F283/12 , C08F283/02 , C08G18/00 , B32B15/08 , B32B15/02 , C08K3/08 , G01N21/00
CPC分类号： B22F1/0018 , B22F1/0062 , B22F1/0096 , B22F2998/00 , B82Y30/00 , C08F8/32 , C08F2800/20 , G01N21/65 , G01N21/658 , Y10T428/2998 , Y10T428/31678 , C08F212/08 , B22F1/0022 , C08F222/06
摘要： The invention relates generally to polyvalent macromolecules comprising: (a) a polymer backbone and (b) pendent groups attached to said polymer backbone, wherein some or all of said pendent groups comprise: (i) optionally a linker, (ii) a surface-seeking group, which is capable of binding strongly to a metal surface, (iii) optionally a chromophore, which is detectable by at least one spectroscopic method. Such molecules can modify metal surfaces and have a variety of uses including (without limitation) appending chromophores to metal surfaces without the need to establish complex chemistry; for the stabilisation and labelling of metal nanoparticles; and for preparing and aggregating nanoparticles in a controlled fashion.
摘要翻译：本发明一般涉及多价大分子，其包含：（a）聚合物主链和（b）与所述聚合物骨架连接的侧基，其中所述侧基中的一些或全部包括：（i）任选的连接体，（ii）能够与金属表面强结合的寻找组，（iii）任选的发色团，其可通过至少一种光谱法检测。这样的分子可以改变金属表面并具有各种用途，包括（但不限于）将发色团附着在金属表面上，而不需要建立复杂的化学物质; 用于稳定和标记金属纳米粒子; 并以可控制的方式制备和聚集纳米颗粒。

3. 发明授权

US09029473B2 Functionalised polymers for binding metal surfaces 有权
标题翻译：用于结合金属表面的官能化聚合物
公开(公告)号：US09029473B2
公开(公告)日：2015-05-12
申请号：US12446817
申请日：2007-10-23
申请人： Peter Cormack , Duncan Graham , Aaron Hernandez-Santana , Arun Prasath Ramaswamy , William Ewen Smith
发明人： Peter Cormack , Duncan Graham , Aaron Hernandez-Santana , Arun Prasath Ramaswamy , William Ewen Smith
IPC分类号： G01N21/65 , C08F8/00 , C08F283/00 , C08G63/91 , C08F283/12 , C08F283/02 , C08G18/00 , B32B15/08 , B32B15/02 , C08K3/08 , B22F1/00 , B82Y30/00
CPC分类号： B22F1/0018 , B22F1/0062 , B22F1/0096 , B22F2998/00 , B82Y30/00 , C08F8/32 , C08F2800/20 , G01N21/65 , G01N21/658 , Y10T428/2998 , Y10T428/31678 , C08F212/08 , B22F1/0022 , C08F222/06
摘要： Disclosed are polyvalent macromolecules, compositions comprising the macromolecules, and methods of use. The polyvalent macromolecules have a polymer backbone and pendent groups attached to the polymer backbone. Some or all of the pendent groups have optionally a linker, a surface-seeking group capable of binding strongly to a metal surface, and a spectroscopically detectable chromophore detectable.
摘要翻译：公开了多价大分子，包含大分子的组合物和使用方法。多价大分子具有聚合物主链和附着于聚合物主链的侧基。侧链基团中的一些或全部可任选地具有能够强烈结合金属表面的接头，表面寻找基团和可检测的可光谱检测的发色团。

4. 发明授权

US07001721B1 Nucleic acid sequence identification 失效
标题翻译：核酸序列鉴定
公开(公告)号：US07001721B1
公开(公告)日：2006-02-21
申请号：US09700732
申请日：1999-05-20
申请人： David Mark Whitcombe , Duncan Graham , William Ewen Smith
发明人： David Mark Whitcombe , Duncan Graham , William Ewen Smith
IPC分类号： C12Q1/68 , C12N15/00 , C12N15/63 , C12N1/20 , C07H21/04
CPC分类号： C12Q1/6825 , G01N21/658 , C12Q2565/632 , C12Q2565/107 , C12Q2563/155
摘要： Disclosed are methods for determining the presence or absence of a target nucleic acid (e.g. DNA) sequence in a sample nucleic acid, the method comprising: (a) exposing the sample to a detection agent comprising a colloid metal surface associated with a SER(R)S active species (SAS) such as an azo dye and with a target binding species (TBS) which may be PNA which is complementary to the target, and (b) observing the sample agent mixture using SER(R)S to detect any surface enhancement of the label wherein the binding of the TBS to the target sequence causes surface enhancement SAS. The detection agent may be exposed to the sample in step (a) as two or more separate components and will generally comprise a first agent and a second agent each having a different TBS, each TBS being capable of binding to the target sequence, and wherein the binding of the first and second TBS to the target sequence brings a metal surface associated with each TBS into proximity thereby causing surface enhancement of an SAS associated with one or both of the metal surfaces.
摘要翻译：公开了用于确定样品核酸中靶核酸（例如DNA）序列的存在或不存在的方法，所述方法包括：（a）将样品暴露于包含与SER（R ）S活性物质（SAS），例如偶氮染料和具有目标结合物质（TBS），其可以是与靶互补的PNA，和（b）使用SER观察样品试剂混合物以检测任何标签的表面增强，其中TBS与靶序列的结合导致表面增强SAS。检测剂可以在步骤（a）中作为两个或更多个单独的组分暴露于样品，并且通常包含每个具有不同TBS的第一试剂和第二试剂，每个TBS能够结合靶序列，其中第一和第二TBS与靶序列的结合使得与每个TBS相关联的金属表面接近，从而引起与一个或两个金属表面相关联的SAS的表面增强。

5. 发明授权

US6127120A Detection of nucleic acids and nucleic acid units 失效
标题翻译：检测核酸和核酸单位
公开(公告)号：US6127120A
公开(公告)日：2000-10-03
申请号：US983486
申请日：1998-04-21
申请人： Duncan Graham , Adrian Matthew Thornton Linacre , Callum Hugh Munro , William Ewan Smith , Nigel Dean Watson , Peter Cyril White
发明人： Duncan Graham , Adrian Matthew Thornton Linacre , Callum Hugh Munro , William Ewan Smith , Nigel Dean Watson , Peter Cyril White
IPC分类号： G01N33/543 , C07D249/18 , C12N15/09 , C12Q1/68 , G01N21/65 , G01N33/566 , C07H19/00 , C07H21/04 , G01N33/553
CPC分类号： C12Q1/6816 , G01N21/658 , Y10T436/24
摘要： The invention relates to the detection of target nucleic acids or nucleic acid units in a sample, by obtaining a SER(R)S spectrum for a SER(R)S-active complex containing, or derived directly from, the target. The complex includes at least a SER(R)S-active label, and optionally a target binding species containing a nucleic acid or nucleic acid unit. In this detection method, the concentration of the target present in the SER(R)S-active complex, or of the nucleic acid or unit contained in the target binding species in the SER(R)S-active complex, is no higher than 10.sup.-10 moles per liter. Additionally or alternatively, one or more of the following features may be used with the method: i) the introduction of a polyamine; ii) modification of the target, and/or of the nucleic acid or nucleic acid unit contained in the target binding species, in a manner that promotes or facilitates its chemi-sorption onto a SER(R)S-active surface; iii) inclusion of a chemi-sorptive functional group in the SER(R)S-active label. The invention also provides SER(R)S-active complexes for use in such a method, a kit for use in carrying out the method or preparing the complexes and a method for sequencing a nucleic acid which comprises the use of the detection method to detect at least one target nucleotide or sequence of nucleotides within the acid.
摘要翻译： PCT No.PCT / GB96 / 01830 Sec。 371日期：1998年4月21日 102（e）1998年4月21日PCT PCT 1996年7月25日PCT公布。出版物WO97 / 05280 日期1997年2月13日本发明涉及检测样品中的靶核酸或核酸单元，通过获得含有或直接从其中衍生的S（S）活性复合物的SER（S）S光谱，目标。所述复合物包括至少一种SER（R）S-活性标记，以及任选的含有核酸或核酸单元的靶结合物质。在该检测方法中，SER（R）S-活性复合物中存在的靶标或SER（S）活性复合物中目标结合物中所含的核酸或单元的浓度不高于 10-10摩尔/升。另外或替代地，可以使用以下方法中的一个或多个以下特征：i）引入多胺; ii）以促进或促进其对SER活性表面的化学吸附的方式修饰靶，和/或包含在靶结合物种中的核酸或核酸单元; iii）在SER（R）S活性标签中包含化学吸附官能团。本发明还提供了用于这种方法的SER（R）S-活性复合物，用于实施该方法或制备复合物的试剂盒和核酸测序方法，其包括使用检测方法检测酸中的至少一个靶核苷酸或核苷酸序列。

6. 发明申请

US20100040661A1 Materials and methods for cell growth 审中-公开
标题翻译：细胞生长的材料和方法
公开(公告)号：US20100040661A1
公开(公告)日：2010-02-18
申请号：US12458425
申请日：2009-07-10
申请人： John A. Hunt , Judith M. Curran , Duncan Graham , Robert Stokes
发明人： John A. Hunt , Judith M. Curran , Duncan Graham , Robert Stokes
IPC分类号： A61F2/00 , C12N11/14 , C12N11/02 , C12N5/02
CPC分类号： C12N5/0068 , C12N5/0655 , C12N2533/12 , C12N2533/14 , C12N2535/10
摘要： Nanopatterned surfaces which provide for improved cell growth including improved stem cell differentiation. The patterned surfaces can comprise an array of fields of biologically active moieties and can be controlled by parameters which include the pitch between the fields and the size of the fields. Nanopatterning can be carried out with use of dip pen nanolithographic printing, microcontact printing, and nanoimprint lithography.
摘要翻译：提供改善的细胞生长的纳米图案表面，包括改进的干细胞分化。图案化表面可以包括生物活性部分的阵列阵列，并且可以通过包括场之间的间距和场的大小的参数来控制。可以使用浸笔纳米光刻印刷，微接触印刷和纳米压印光刻进行纳米图案化。

7. 发明申请

US20050130163A1 Serrs reactive particles 审中-公开
标题翻译： Serrs反应性颗粒
公开(公告)号：US20050130163A1
公开(公告)日：2005-06-16
申请号：US10507717
申请日：2003-07-11
申请人： William Smith , Duncan Graham , Peter Cormack , Ailie McCabe
发明人： William Smith , Duncan Graham , Peter Cormack , Ailie McCabe
IPC分类号： G01N21/65 , B01J13/14 , C07B61/00 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , C12Q1/6816 , G01N33/53 , G01N33/543 , G01N33/553 , G01N33/566 , G01N33/58
CPC分类号： G01N33/587 , B01J13/14 , B82Y15/00 , C07B2200/11 , C12Q1/6816 , C40B20/04 , G01N21/658 , C12Q2563/149 , C12Q2563/137
摘要： The present invention relates to the provision of SERRS active polymer beads and a method of their production for use in detecting targect molecules, as well as methods of detecting target molecules.
摘要翻译：本发明涉及提供SERRS活性聚合物珠粒及其制备用于检测分子量的方法，以及检测靶分子的方法。

8. 发明申请

US20120058471A1 IDENTIFICATION OF NUCLEIC ACID SEQUENCES 审中-公开
标题翻译：核酸序列的鉴定
公开(公告)号：US20120058471A1
公开(公告)日：2012-03-08
申请号：US12673183
申请日：2008-08-13
申请人： Duncan Graham , Karen Faulds , Ewen Smith , Alastair Ricketts
发明人： Duncan Graham , Karen Faulds , Ewen Smith , Alastair Ricketts
IPC分类号： C12Q1/68 , B82Y15/00
CPC分类号： C12Q1/6823 , C12Q2565/632 , C12Q2563/107 , C12Q2521/319
摘要： The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said target nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex; and (iii) detecting the label by Raman spectroscopy.
摘要翻译：本发明提供了一种用于检测靶核酸的方法，包括以下步骤：（i）在有效通过特异性产生探针/靶核酸双链体的条件下，将单链探针核酸与感兴趣的样品接触如果存在所述靶核酸，则所述探针核酸与靶核酸的杂交; （ii）使任何探针/靶核酸双链体与外切核酸酶接触以实现双链体的消化和从双链体释放标记分子; 和（iii）通过拉曼光谱法检测标记物。

9. 发明申请

US20060246460A1 Nucleic acid sequence identification 审中-公开
标题翻译：核酸序列鉴定
公开(公告)号：US20060246460A1
公开(公告)日：2006-11-02
申请号：US10569698
申请日：2004-08-26
申请人： Duncan Graham , William Smith , Ljiljana Fruk
发明人： Duncan Graham , William Smith , Ljiljana Fruk
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： G01N21/658 , G01J3/44 , G01N2021/653
摘要： The invention provides modified molecular beacons detectable by surface enhanced Raman spectroscopy (SERS) and related materials, processes, and methods of use. Examples methods provide for the determination of the presence or absence of a target nucleotide sequence in a sample nucleic acid by (a) providing a detection agent, which agent comprises: (i) a probe comprising a target complement sequence (TCS) being complementary to the target sequence and flanking the TCS, first and second oligonucleotide arms, said first and second oligonucleotide arms forming a stem duplex, and said first arm incorporating a first label moiety being detectable by SERS (e.g. a fluoroscein dye) and said second arm terminating in a second label moiety being detectable by SERS, which second arm further includes a surface seeking group (SSG—e.g. an azo-benzotriazole) capable of promoting association of the second label onto an enhancing surface ii) associated with said probe via said SSG, an enhancing surface, such that said first and second label moieties are in close proximity to each other and to the enhancing surface (b) exposing the sample to the detection agent, (c) detecting hybridisation of the TCS to any target sequence present in the sample by a change in the SERS spectra of said agent.
摘要翻译：本发明提供通过表面增强拉曼光谱（SERS）和相关材料，方法和使用方法可检测的修饰分子信标。实例方法通过（a）提供检测剂来确定样品核酸中靶核苷酸序列的存在或不存在，所述检测剂包括：（i）包含与SEQ ID NO：1的互补序列的靶互补序列（TCS）的探针所述靶序列和所述TCS第一和第二寡核苷酸臂的侧翼，所述第一和第二寡核苷酸臂形成茎双相，并且所述第一臂结合第一标记部分可被SERS（例如氟代染料染料）检测，所述第二臂终止于第二标记部分可由SERS检测，该第二臂还包括能够促进第二标记与经由所述SSG与所述探针相关联的增强表面ii）的表面寻找组（SSG，例如偶氮 - 苯并三唑），使得所述第一和第二标签部分彼此靠近并且与所述增强表面（b）将样品暴露于检测剂，（c）通过所述试剂的SERS光谱的变化来检测TCS与样品中存在的任何靶序列的杂交。

10. 发明授权

US08632964B2 Detection system 有权
标题翻译：检测系统
公开(公告)号：US08632964B2
公开(公告)日：2014-01-21
申请号：US12475141
申请日：2009-05-29
申请人： Robert Stokes , Duncan Graham
发明人： Robert Stokes , Duncan Graham
IPC分类号： C12Q1/00
CPC分类号： C12Q1/00 , G01N21/658 , G01N33/54373 , G01N33/6803
摘要： The present invention relates to methods of making nanoarrays for use in detecting species formed on the surface of the array using SE(R)RS. The methods can involve nanolithographic printing of a compound by dip pen nanolithographic printing. A SE(R)RS substrate can be used for the array and which can be selected from surfaces roughened by the oxidation-reduction cycle (ORC), island films, colloidal nanoparticles and surface-confined nanostructures. A coating or intermediate layer, such as a layer formed of nitrocellulose, can be provided between the compound and the SE(R)RS substrate. There are also provided arrays themselves and methods of using such arrays.
摘要翻译：本发明涉及使用SE（RS）RS制备用于检测在阵列表面上形成的物质的纳米阵列的方法。这些方法可以涉及通过浸渍笔纳米光刻印刷的化合物的纳米光刻印刷。可以使用SE（R）RS衬底作为阵列，其可以从通过氧化还原循环（ORC），岛膜，胶体纳米颗粒和表面限制的纳米结构粗糙化的表面中选择。可以在化合物和SE（R）RS底物之间提供涂层或中间层，例如由硝酸纤维素形成的层。还提供了数组本身和使用这些数组的方法。

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