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1. 发明授权

US5288647A Method of irradiating biological specimens 失效
标题翻译：辐射生物标本的方法
公开(公告)号：US5288647A
公开(公告)日：1994-02-22
申请号：US686491
申请日：1991-04-17
申请人： William C. Zimlich, Jr. , Joseph A. Sorge
发明人： William C. Zimlich, Jr. , Joseph A. Sorge
IPC分类号： C07K17/00 , C12Q1/68 , G01J1/04
CPC分类号： C07K17/00 , C12Q1/68 , Y10T436/25
摘要： A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a substrate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.
摘要翻译：用紫外线照射生物样本的方法，特别是选自DNA或RNA的多核苷酸标本，或任选的蛋白质。在标本是DNA或RNA或潜在的蛋白质的情况下，照射样品以将样品交联到基底上。在标本为DNA的情况下，也可以照射样品以形成胸腺嘧啶二聚体。该方法使用允许相对精确地控制由样品接收的总紫外线剂量的装置，尽管在任何一个实验期间或在多个实验之间可能发生来自灯的紫外线通量的任何变化。因此，该方法允许获得相对高度可重复的结果。

2. 发明授权

US5814523A Method of irradiating biological specimens 失效
标题翻译：辐射生物标本的方法
公开(公告)号：US5814523A
公开(公告)日：1998-09-29
申请号：US896717
申请日：1997-07-18
申请人： William C. Zimlich, Jr. , Joseph A. Sorge
发明人： William C. Zimlich, Jr. , Joseph A. Sorge
IPC分类号： C07K17/00 , C12Q1/68 , G01J1/04
CPC分类号： C07K17/00 , C12Q1/68 , Y10T436/25
摘要： A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a substrate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.
摘要翻译：用紫外线照射生物样本的方法，特别是选自DNA或RNA的多核苷酸标本，或任选的蛋白质。在标本是DNA或RNA或潜在的蛋白质的情况下，照射样品以将样品交联到基底上。在标本为DNA的情况下，也可以照射样品以形成胸腺嘧啶二聚体。该方法使用允许相对精确地控制由样品接收的总紫外线剂量的装置，尽管在任何一个实验期间或在多个实验之间可能发生来自灯的紫外线通量的任何变化。因此，该方法允许获得相对高度可重复的结果。

3. 发明授权

US5395591A Apparatus of irradiating biological specimens 失效
标题翻译：照射生物标本的装置
公开(公告)号：US5395591A
公开(公告)日：1995-03-07
申请号：US999434
申请日：1992-12-30
申请人： William C. Zimlich, Jr. , Joseph A. Sorge
发明人： William C. Zimlich, Jr. , Joseph A. Sorge
IPC分类号： C07K17/00 , C12Q1/68 , G05D25/02
CPC分类号： C07K17/00 , C12Q1/68 , Y10T436/25
摘要： A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a substrate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.
摘要翻译：用紫外线照射生物样本的方法，特别是选自DNA或RNA的多核苷酸标本，或任选的蛋白质。在标本是DNA或RNA或潜在的蛋白质的情况下，照射样品以将样品交联到基底上。在标本为DNA的情况下，也可以照射样品以形成胸腺嘧啶二聚体。该方法使用允许相对精确地控制由样品接收的总紫外线剂量的装置，尽管在任何一个实验期间或在多个实验之间可能发生来自灯的紫外线通量的任何变化。因此，该方法允许获得相对高度可重复的结果。

4. 发明授权

US08383343B2 Methods of enriching for and identifying polymorphisms 有权
标题翻译：富集和鉴定多态性的方法
公开(公告)号：US08383343B2
公开(公告)日：2013-02-26
申请号：US10391090
申请日：2003-03-18
申请人： Joseph A. Sorge
发明人： Joseph A. Sorge
IPC分类号： C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6827 , C12Q1/6869 , C12Q2565/519 , C12Q2565/531 , C12Q2533/101 , C12Q2522/101
摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.
摘要翻译：本发明包括用于通过分离核酸样品的子集或通过选择性复制核酸样品的子集来使得核酸样品中的多态性富集和鉴定核酸样品中的多态性的方法，使得多态性包含在具有降低的核酸群体的核酸群体内复杂性，然后鉴定富集的核酸样品内的多态性。公开了通过使包含具有与序列特异性结合活性结合的序列的核酸分子的子集与具有序列特异性结合活性的分子的核酸样品接触的方法来丰富和鉴定多态性的方法，允许特异性结合，使得与活性结合的核酸分子的子集富含具有由序列特异性结合活性识别的序列的核酸分子，并且检测核酸子集中的参考序列的多态性分子。

5. 发明授权

US07858359B2 Method for tapping the immunological repertoire 失效
标题翻译：攻击免疫学曲目的方法
公开(公告)号：US07858359B2
公开(公告)日：2010-12-28
申请号：US11558384
申请日：2006-11-09
申请人： William D. Huse , Gregory P. Winter , Lutz Riechmann , Joseph A. Sorge , Richard A. Lerner
发明人： William D. Huse , Gregory P. Winter , Lutz Riechmann , Joseph A. Sorge , Richard A. Lerner
IPC分类号： C12N1/21
CPC分类号： C07K16/00 , A61K38/00 , C07K16/468 , C07K2317/56
摘要： The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.
摘要翻译：本发明涉及从免疫基因库中分离编码具有结合预选配体的能力的受体的基因的方法。还考虑了通过该方法分离的基因产生的受体，特别是催化受体。

6. 发明申请

US20100248246A1 METHODS OF ENRICHING FOR AND IDENTIFYING POLYMORPHISMS 审中-公开
标题翻译：增强和鉴别多态性的方法
公开(公告)号：US20100248246A1
公开(公告)日：2010-09-30
申请号：US12749070
申请日：2010-03-29
申请人： Joseph A. Sorge , Ronald W. Davis
发明人： Joseph A. Sorge , Ronald W. Davis
IPC分类号： C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6827 , C12Q1/6869 , C12Q2565/519 , C12Q2565/531 , C12Q2533/101 , C12Q2522/101
摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.
摘要翻译：本发明包括用于通过分离核酸样品的子集或通过选择性复制核酸样品的子集来使得核酸样品中的多态性富集和鉴定核酸样品中的多态性的方法，使得多态性包含在具有降低的核酸群体的核酸群体内复杂性，然后鉴定富集的核酸样品内的多态性。公开了通过使包含具有与序列特异性结合活性结合的序列的核酸分子的子集与具有序列特异性结合活性的分子的核酸样品接触的方法来丰富和鉴定多态性的方法，允许特异性结合，使得与活性结合的核酸分子的子集富含具有由序列特异性结合活性识别的序列的核酸分子，并且检测核酸子集中的参考序列的多态性分子。

7. 发明授权

US07585632B2 Compositions and methods for the detection of a nucleic acid using a cleavage reaction 有权
标题翻译：使用裂解反应检测核酸的组合物和方法
公开(公告)号：US07585632B2
公开(公告)日：2009-09-08
申请号：US11699736
申请日：2007-01-29
申请人： Joseph A. Sorge , Andrew Firmin , Scott Happe
发明人： Joseph A. Sorge , Andrew Firmin , Scott Happe
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6844 , C12Q1/6823 , C12Q2525/307 , C12Q2565/101 , C12Q2521/301 , C12Q2525/161
摘要： The invention relates to compositions and methods for generating a signal indicative of the presence of a target nucleic acid in a sample utilizing a primer-probe duplex.
摘要翻译：本发明涉及使用引物 - 探针双链体产生指示样品中靶核酸存在的信号的组合物和方法。

8. 发明申请

US20080199935A1 Compositions and methods utilizing DNA polymerases 审中-公开
标题翻译：使用DNA聚合酶的组合物和方法
公开(公告)号：US20080199935A1
公开(公告)日：2008-08-21
申请号：US11644729
申请日：2006-12-22
申请人： Joseph A. Sorge , Holly Hogrefe , Connie J. Hansen
发明人： Joseph A. Sorge , Holly Hogrefe , Connie J. Hansen
IPC分类号： C12N9/12
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
摘要翻译：本发明的特征在于新的分离的家族B DNA聚合酶，热球菌聚合酶JDF-3及其突变体重组形式。本发明的突变型聚合酶相对于野生型形式的聚合酶，缺乏3'至5'核酸外切酶活性和/或表现出对非常规核苷酸的降低的鉴别。

9. 发明授权

US07361467B2 Methods for detection of a target nucleic acid by capture 有权
标题翻译：通过捕获检测靶核酸的方法
公开(公告)号：US07361467B2
公开(公告)日：2008-04-22
申请号：US11150775
申请日：2005-06-10
申请人： Joseph A. Sorge , Anne M. Whalen
发明人： Joseph A. Sorge , Anne M. Whalen
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6823 , C12Q1/6816 , C12Q2521/301 , C12Q2531/113 , C12Q2521/307 , C12Q2525/161 , C12Q2525/301 , C12Q2561/109 , C12Q2565/519 , C12Q2561/101 , C12Q2521/107
摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid and further comprising a binding moiety. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample, and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.The invention also relates to a method of detecting or measuring a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to a target nucleic acid and comprising a binding moiety, and cleaving the cleavage structure with a nuclease to generate a cleaved nucleic acid fragment and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.
摘要翻译：本发明涉及产生指示样品中目标核酸存在的信号的方法，其中该方法包括通过将包含靶核酸的样品与具有二级结构变化的探针一起孵育形成切割结构探针与靶核酸的结合并且还包含结合部分。本发明还包括用核酸酶切割切割结构以释放核酸片段以产生信号的步骤，其中产生信号指示样品中靶核酸的存在，以及检测和/或测量通过结合部分与固体支持物上的捕获元件结合捕获的片段的量。本发明还涉及一种检测或测量样品中的靶核酸的方法，其中该方法包括通过将含有靶核酸的样品与具有在探针结合后变化的二级结构的探针形成切割结构并且包含结合部分，并用核酸酶切割切割结构以产生切割的核酸片段，并通过结合部分与捕获元件的结合捕获的片段的量，并检测和/或测量坚实的支持。

10. 发明授权

US07109178B2 Method for ligating nucleic acids and molecular cloning 有权
公开(公告)号：US07109178B2
公开(公告)日：2006-09-19
申请号：US10057050
申请日：2002-01-25
申请人： Henry Ji , Alan Greener , Joseph A. Sorge , John Bauer , Richard Gibbs , Carsten-Peter Carstens
发明人： Henry Ji , Alan Greener , Joseph A. Sorge , John Bauer , Richard Gibbs , Carsten-Peter Carstens
IPC分类号： A61K31/711
CPC分类号： C12N9/00 , C12N15/10 , C12N15/64 , C12N15/66 , C12P19/34
摘要： The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.

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