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1. 发明授权

US07361467B2 Methods for detection of a target nucleic acid by capture 有权
标题翻译：通过捕获检测靶核酸的方法
公开(公告)号：US07361467B2
公开(公告)日：2008-04-22
申请号：US11150775
申请日：2005-06-10
申请人： Joseph A. Sorge , Anne M. Whalen
发明人： Joseph A. Sorge , Anne M. Whalen
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6823 , C12Q1/6816 , C12Q2521/301 , C12Q2531/113 , C12Q2521/307 , C12Q2525/161 , C12Q2525/301 , C12Q2561/109 , C12Q2565/519 , C12Q2561/101 , C12Q2521/107
摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid and further comprising a binding moiety. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample, and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.The invention also relates to a method of detecting or measuring a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to a target nucleic acid and comprising a binding moiety, and cleaving the cleavage structure with a nuclease to generate a cleaved nucleic acid fragment and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.
摘要翻译：本发明涉及产生指示样品中目标核酸存在的信号的方法，其中该方法包括通过将包含靶核酸的样品与具有二级结构变化的探针一起孵育形成切割结构探针与靶核酸的结合并且还包含结合部分。本发明还包括用核酸酶切割切割结构以释放核酸片段以产生信号的步骤，其中产生信号指示样品中靶核酸的存在，以及检测和/或测量通过结合部分与固体支持物上的捕获元件结合捕获的片段的量。本发明还涉及一种检测或测量样品中的靶核酸的方法，其中该方法包括通过将含有靶核酸的样品与具有在探针结合后变化的二级结构的探针形成切割结构并且包含结合部分，并用核酸酶切割切割结构以产生切割的核酸片段，并通过结合部分与捕获元件的结合捕获的片段的量，并检测和/或测量坚实的支持。

2. 发明授权

US07118860B2 Methods for detection of a target nucleic acid by capture 有权
标题翻译：通过捕获检测靶核酸的方法
公开(公告)号：US07118860B2
公开(公告)日：2006-10-10
申请号：US09728574
申请日：2000-11-30
申请人： Joseph A. Sorge , Anne M. Whalen
发明人： Joseph A. Sorge , Anne M. Whalen
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6823 , C12Q1/6816 , C12Q2521/301 , C12Q2531/113 , C12Q2521/307 , C12Q2525/161 , C12Q2525/301 , C12Q2561/109 , C12Q2565/519 , C12Q2561/101 , C12Q2521/107
摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, as well as a method of detecting or measuring a target nucleic acid in a sample. The invention comprises forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid and further comprising a binding moiety. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample, and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.
摘要翻译：本发明涉及产生指示样品中靶核酸存在的信号的方法，以及检测或测量样品中靶核酸的方法。本发明包括通过将包含靶核酸的样品与具有二级结构的探针一起孵育形成切割结构，所述探针在探针与靶核酸结合后发生变化，并且还包含结合部分。本发明还包括用核酸酶切割切割结构以释放核酸片段以产生信号的步骤，其中产生信号指示样品中靶核酸的存在，以及检测和/或测量通过结合部分与固体支持物上的捕获元件结合捕获的片段的量。

3. 发明授权

US07482121B2 Methods for detection of a target nucleic acid by capture 有权
公开(公告)号：US07482121B2
公开(公告)日：2009-01-27
申请号：US11106237
申请日：2005-04-14
申请人： Joseph A. Sorge , Anne M. Whalen
发明人： Joseph A. Sorge , Anne M. Whalen
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6823 , C12Q1/6816 , C12Q2521/301 , C12Q2531/113 , C12Q2521/307 , C12Q2525/161 , C12Q2525/301 , C12Q2561/109 , C12Q2565/519 , C12Q2561/101 , C12Q2521/107
摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid and further comprising a binding moiety. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample, and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.The invention also relates to a method of detecting or measuring a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to a target nucleic acid and comprising a binding moiety, and cleaving the cleavage structure with a nuclease to generate a cleaved nucleic acid fragment and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.

4. 发明申请

US20080293157A1 Apparatus and method of performing high-throughput cell-culture studies on biomaterials 审中-公开
标题翻译：对生物材料进行高通量细胞培养研究的装置和方法
公开(公告)号：US20080293157A1
公开(公告)日：2008-11-27
申请号：US11753063
申请日：2007-05-24
申请人： Gerald Frederickson , Adrian McNamara , Rachit Ohri , Todd R. Robida , Anne M. Whalen
发明人： Gerald Frederickson , Adrian McNamara , Rachit Ohri , Todd R. Robida , Anne M. Whalen
IPC分类号： G01N1/28 , C12M1/00 , C12M1/34
CPC分类号： C12M23/12 , B01L3/5085 , B01L3/50853 , B01L3/50855 , B01L2200/0689 , B01L2300/0822 , B01L2300/0829 , B01L2300/0851 , Y10T436/25
摘要： In one embodiment, a kit includes a base and a specimen removably couplable to the base. A top plate defines a plurality of apertures and is removably couplable to the base such that at least two of the apertures are associated with a specimen and each aperture defines a well with that specimen. Each well is configured to receive a sample material therein in contact with the specimen. A method includes disposing a specimen within a recessed portion of a test apparatus and coupling a top plate of the test apparatus to a base of the test apparatus such that a sealing engagement is formed between the top plate and the specimen. The top plate defines a plurality of apertures, each of at least two of the apertures collectively with the specimen defines a well. A sample material can be disposed within at least one well.
摘要翻译：在一个实施例中，套件包括基部和可移除地联接到基部的样本。顶板限定多个孔，并且可移除地联接到基部，使得至少两个孔与样本相关联，并且每个孔与该标本形成一个孔。每个孔被构造成接收与样品接触的样品材料。一种方法包括将试样设置在测试装置的凹陷部分内，并将测试装置的顶板连接到测试装置的底部，使得在顶板和试样之间形成密封接合。顶板限定多个孔，与样本共同的至少两个孔中的每一个限定了孔。样品材料可以设置在至少一个孔内。

5. 发明授权

US07189508B2 Methods for detection of a target nucleic acid by capture using multi-subunit probes 失效
标题翻译：使用多亚基探针捕获检测靶核酸的方法
公开(公告)号：US07189508B2
公开(公告)日：2007-03-13
申请号：US10197026
申请日：2002-07-17
申请人： Joseph Sorge , Anne M. Whalen
发明人： Joseph Sorge , Anne M. Whalen
IPC分类号： C21Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6823 , C12Q2537/1373 , C12Q2537/137 , C12Q2565/1015 , C12Q2537/1376
摘要： The invention relates to generating a signal indicative of a target nucleic acid sequence, comprising forming a complex by incubating a sample comprising a target nucleic acid sequence with a probe comprising a first and second subunit, and a binding moiety, and dissociating the first and second subunit to release the first subunit and generate a signal. The invention also relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising forming a complex by incubating a target nucleic acid sequence, an upstream primer and a probe comprising a first and second subunit, and a binding moiety. The primer is extended with a nucleic acid polymerase to displace a portion of the first subunit from the target nucleic acid strand thereby dissociating the first subunit from the second subunit to release the first subunit and generate a signal.
摘要翻译：本发明涉及产生指示靶核酸序列的信号，包括通过将包含靶核酸序列的样品与包含第一和第二亚基的探针和结合部分一起孵育而形成复合物，并解离第一和第二子单元释放第一个子单元并产生信号。本发明还涉及产生指示样品中靶核酸序列存在的信号的方法，包括通过孵育靶核酸序列，上游引物和包含第一和第二亚基的探针形成复合物，和结合部分。用核酸聚合酶扩增引物以从目标核酸链取代部分第一亚基，从而将第一亚基与第二亚基解离以释放第一亚基并产生信号。

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