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1. 发明申请

WO0236791A2 NUCLEUS EXPORT REPORTER SYSTEM 审中-公开
标题翻译：核出口报告系统
公开(公告)号：WO0236791A2
公开(公告)日：2002-05-10
申请号：PCT/EP0112534
申请日：2001-10-30
申请人： WAGNER RALF , GRAF MARCUS
发明人： WAGNER RALF , GRAF MARCUS
IPC分类号： C12N15/85 , C12Q1/68 , C12N15/86
CPC分类号： C12N15/1051 , C12N15/1075 , C12N15/85 , C12N2840/44 , C12Q1/6897
摘要： The invention relates to reporter systems for RNA export, to a method for locating molecules which influence RNA export and to a technique for detecting a virus infection which is based on this method.
摘要翻译：本发明涉及一种用于RNA输出，处理用于在搜索了影响RNA分子出口报道体系，并且基于这些方法中，用于检测病毒感染的方法。

2. 发明申请

WO2004059556A3 METHOD AND DEVICE FOR OPTIMIZING A NUCLEOTIDE SEQUENCE FOR THE PURPOSE OF EXPRESSION OF A PROTEIN 审中-公开
标题翻译： VERFAREN和用于表达蛋白质优化的核苷酸序列装置
公开(公告)号：WO2004059556A3
公开(公告)日：2005-12-01
申请号：PCT/EP0314850
申请日：2003-12-23
申请人： GENEART GMBH , RAAB DAVID , GRAF MARCUS , NOTKA FRANK , WAGNER RALF
发明人： RAAB DAVID , GRAF MARCUS , NOTKA FRANK , WAGNER RALF
IPC分类号： C07H21/00 , C12M1/36 , C12N15/11 , C12N15/12 , C12N15/19 , C12N15/24 , C12N15/27 , C12N15/29 , C12N15/31 , C12N15/52 , C12P21/00 , G06F19/18 , G06F19/22 , G06F19/00
CPC分类号： G06F19/20 , C07K14/43595 , C07K14/535 , C07K14/5443 , G06F19/18 , G06F19/22
摘要： The invention relates to a method for optimizing a nucleotide sequence for the purpose of expression of a protein on the basis of the amino acid sequence of said protein. According to the inventive method, a test sequence with m optimization positions is determined for a defined region, in which positions the codon usage is varied. The optimum codon usage on said optimization positions is determined by means of a power function and one or more codons of said optimum usage are determined as the codons of the optimized nucleotide sequence. These steps are iterated, while the codons of the optimized nucleotide sequence determined in the preceding steps remain unchanged during the subsequent iteration steps. The invention further relates to a device for carrying out said method.
摘要翻译：本发明涉及一种方法，用于该蛋白质的氨基酸序列，其中对于给定的范围内，其中m优化位置的测试序列来确定在其上的密码子职业多种多样，通过一个质量函数，最佳密码子占领上的装置的基础上表达蛋白质优化的核苷酸序列这种优化的位置被确定，并且一个或多个密码子优化本职业被定义为密码子优化的核苷酸序列。这些步骤被重复，从而在前面的步骤密码子的组优化的核苷酸序列保持在随后的迭代步骤不变。本发明还涉及一种用于执行这种方法。

3. 发明申请

WO2006015789A2 METHOD FOR MODULATING GENE EXPRESSION BY MODIFYING THE CPG CONTENT 审中-公开
标题翻译：调制基因表达的方法通过修改CpG含量
公开(公告)号：WO2006015789A2
公开(公告)日：2006-02-16
申请号：PCT/EP2005008423
申请日：2005-08-03
申请人： GENEART GMBH , NOTKA FRANK , GRAF MARCUS , LEIKAM DORIS , WAGNER RALF , RAAB DAVID
发明人： NOTKA FRANK , GRAF MARCUS , LEIKAM DORIS , WAGNER RALF , RAAB DAVID
IPC分类号： C12N15/63
CPC分类号： C12N15/63 , C07K14/43595 , C12N2830/46
摘要： The invention relates to nucleic acid modifications for a directed expression modulation by the targeted insertion or removal of CpG dinucleotides. The invention also relates to modified nucleic acids and expression vectors.
摘要翻译：本发明涉及核酸用于通过CpG二核苷酸的靶向插入或移除的表达的定向调制氨基酸修饰。此外，本发明涉及modifzierte核酸和表达载体。

4. 发明申请

WO0136614A2 THE GENOME OF THE HIV-1 INTER-SUBTYPE (C/B') AND USE THEREOF 审中-公开
标题翻译：的HIV-1亚型INTER（C / B“）及其应用的基因组中
公开(公告)号：WO0136614A2
公开(公告)日：2001-05-25
申请号：PCT/DE0004073
申请日：2000-11-16
申请人： GENEART GMBH GESSELLSCHAFT FUE , SHAO YIMING , WAGNER RALF , WOLF HANS , GRAF MARCUS
发明人： SHAO YIMING , WAGNER RALF , WOLF HANS , GRAF MARCUS
IPC分类号： A61K39/00 , C07K14/16 , C12N15/00
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/53 , C12N2740/16122 , C12N2740/16222 , C12N2740/16322
摘要： The invention relates to a polynucleotide, comprising a nucleic acid sequence according to SEQ ID NO:1, 2 or 3, fragments or derivatives thereof, or a polynucleotide hybridised with the nucleic acid sequence according to SEQ ID NO:1, 2 or 3. The invention further relates to polypeptides coded from said nucleotide sequence, or fragment, or derivative of the nucleic acid sequence according to SEQ ID NO:1, 2 or 3. The polynucleotides and polypeptides may be used as medicaments, vaccines or diagnostics, in particular for the treatment, prophylaxis and diagnosis of HIV infections.
摘要翻译：本发明涉及包含根据SEQ ID NO的核酸序列的多核苷酸：1，2或3或其片段或衍生物，或具有根据SEQ ID NO的核酸序列的多核苷酸：1，2或第三本发明进一步涉及由SEQ ID NO：1所示的核苷酸序列，或其片段或衍生物的核苷酸序列的编码的多肽：1，2或3的多核苷酸和多肽可以用作药物，疫苗或诊断试剂，尤其是对HIV的治疗，预防和诊断感染可以使用。

5. 发明申请

WO2008115504A3 NUCLEIC ACIDS ENCODING HUMANIZED IMMUNOGLOBULIN THAT BINDS A4B7 INTEGRIN 审中-公开
标题翻译：编码人体免疫球蛋白的核酸编码A4B7 INTEGRIN
公开(公告)号：WO2008115504A3
公开(公告)日：2009-03-12
申请号：PCT/US2008003560
申请日：2008-03-19
申请人： MILLENNIUM PHARM INC , LI PING , GRAF MARCUS
发明人： LI PING , GRAF MARCUS
IPC分类号： C07K16/28
CPC分类号： C07K16/2839 , C07K2317/24
摘要： The invention relates to an isolated nucleic acid encoding a humanized immunoglobulin that has binding specificity for a4ß7 integrin and comprises the complementarity determining regions (CDRs) of mouse Act-1 antibody. The present invention further relates to an isolated nucleic acid encoding a humanized heavy chain and an isolated nucleic acid encoding a humanized light chain. The invention also relates to recombinant vectors and host cells that comprise a nucleic acid which encodes a humanized immunoglobulin, humanized immunoglobulin heavy chain or a humanized immunoglobulin light chain, and to methods of preparing a humanized immunoglobulin.
摘要翻译：本发明涉及编码对Aβ4整联蛋白具有结合特异性并包含小鼠Act-1抗体的互补决定区（CDR）的人源化免疫球蛋白的分离的核酸。本发明还涉及编码人源化重链的分离的核酸和编码人源化轻链的分离的核酸。本发明还涉及包含编码人源化免疫球蛋白，人源化免疫球蛋白重链或人源化免疫球蛋白轻链的核酸的重组载体和宿主细胞，以及制备人源化免疫球蛋白的方法。

6. 发明专利

AU2481002A Nucleus export reporter system 未知
公开(公告)号：AU2481002A
公开(公告)日：2002-05-15
申请号：AU2481002
申请日：2001-10-30
申请人： WAGNER RALF
发明人： WAGNER RALF , GRAF MARCUS
IPC分类号： C12N15/85 , C12Q1/68 , C12Q1/6897 , C12N15/86
摘要： Detecting RNA export from the nucleus of a eukaryotic cell, comprising preparing a nucleic acid (I) that encodes a reporter protein (RP) and produces a transcript that can be exported from the nucleus, introducing (I) into the nucleus so that, in the nucleus, it is operably linked to a transcription control sequence, transcription from (I), and determining if the transcript is exported, is new. Detecting RNA export from the nucleus of a eukaryotic cell, comprising preparing a nucleic acid (I) that encodes a reporter protein (RP) and produces a transcript that can be exported from the nucleus, introducing (I) into the nucleus so that, in the nucleus, it is operably linked to a transcription control sequence, transcription from (I), and determining if the transcript is exported, is new. Export of the transcript depends on presence of a cis-active RNA export signal, operably linked to (I), a trans-activating factor, and optionally a functional 5'-splice donor, in absence of 3'-splice acceptor. The RP-encoding segment of the transcript can not undergo splicing. Independent claims are also included for the following: (1) DNA sequence (II) that contains RP-encoding sequence, modified from the wild type so that, unlike the wild type, RNA export occurs, depending on a linked cis-acting RNA export element, and optionally a functional 5'-splice donor in absence of 3'-splice acceptor; (2) eukaryotic cell containing (II) in transcribable form; (3) reagent kit comprising (II) or cell transfected by it, and/or the cells of (2); (4) detecting viral infection by detecting activity of a viral nuclear export factor; (5) DNA sequence (III) encoding a fluorescent protein (FP), that is at least partly codon-optimized for a retrovirus, specifically human immune deficiency virus (HIV)-1; (6) DNA sequence (IV) comprising a sequence encoding FP between functional 5'-splice donor and 3'-splice acceptor sites; and (7) detecting RNA export from nuclei of eukaryotic cells, using an RP that can be detected without lysis or fixing of cells.

7. 发明专利

DE10053781A1 未知
公开(公告)号：DE10053781A1
公开(公告)日：2002-05-08
申请号：DE10053781
申请日：2000-10-30
申请人： WAGNER RALF
发明人： GRAF MARCUS , WAGNER RALF
IPC分类号： C12N15/85 , C12Q1/68 , C12Q1/6897 , C12N15/52 , C12N15/79 , C12N7/00
摘要： The invention relates to reporter systems for RNA export, to a method for locating molecules which influence RNA export and to a technique for detecting a virus infection which is based on this method.

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