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1. 发明授权

US5589401A Light scatter-based immunoassay without particle self aggregation 失效
标题翻译：基于光散射的免疫测定，无颗粒自聚集
公开(公告)号：US5589401A
公开(公告)日：1996-12-31
申请号：US286778
申请日：1994-08-05
申请人： W. Peter Hansen , Michael Cennerazzo
发明人： W. Peter Hansen , Michael Cennerazzo
IPC分类号： G01N15/02 , G01N15/14 , G01N33/543 , G01N33/553 , G01N33/538 , G01N33/544
CPC分类号： G01N15/14 , G01N33/54313 , G01N33/553 , G01N15/0205
摘要： A homogeneous immunoassay method for the simultaneous determination of one or more antibody, antigen or hapten analytes in a fluid sample, that comprises the quantification of the effect of said analytes on the statistical changes in a dimension of a light scatter pulse height distribution histogram of relatively large diameter monodisperse binding molecule-coated polymeric microspheres induced by the binding to said microspheres of polydisperse binding molecule-coated colloid metal particles of relatively small diameter. For simultaneous assays of multiple analytes, different diameter or refractive index microspheres are assigned to each analyte. The assay may be used in forward binding, displacement, inhibition, and competition type systems, with the direction of the change in histogram dimension depending on the system. A convenient dimension to measure is the normalized peak width of a graphical representation of the histogram. For simultaneous assays for multiple analytes, a monodisperse polymeric microsphere of unique diameter or refractive index is dedicated to each analyte, so as to generate multiple histograms, one for each analyte. Polydisperse binding molecule-coated colloid metal particles, when used in effective concentrations, will also serve as a scavenger means to reduce the interfering effects of non-specific substances in the analyte-containing fluid samples, particularly when said samples are of biological origin.
摘要翻译：用于同时测定流体样品中一种或多种抗体，抗原或半抗原分析物的均匀免疫测定方法，其包括对所述分析物对相对较轻的光散射脉冲高度分布直方图的维度的统计变化的量化的量化通过与所述微球体结合相对小直径的多分散结合分子包被的胶体金属颗粒诱导的大直径单分散结合分子包被的聚合物微球。对于多个分析物的同时测定，将不同直径或折射率微球分配给每个分析物。该测定可用于正向结合，置换，抑制和竞争类型系统，其中取决于系统的直方图尺寸的变化方向。一个方便的测量尺寸是直方图的图形表示的归一化峰宽。对于多个分析物的同时测定，独特直径或折射率的单分散聚合物微球专用于每个分析物，以便产生多个直方图，每个分析物一个。当以有效浓度使用时，多分散结合分子涂覆的胶体金属颗粒也将用作清除剂手段，以减少非特异性物质在含分析物的流体样品中的干扰作用，特别是当所述样品是生物来源时。

2. 发明授权

US06200820B1 Light scatter-based immunoassay 失效
标题翻译：基于光散射的免疫测定
公开(公告)号：US06200820B1
公开(公告)日：2001-03-13
申请号：US08473187
申请日：1995-06-07
申请人： W. Peter Hansen , Michael Cennerazzo , Carl Theodore Edens , Manish Kochar
发明人： W. Peter Hansen , Michael Cennerazzo , Carl Theodore Edens , Manish Kochar
IPC分类号： G01N33543
CPC分类号： G01N33/54346 , G01N15/0205 , G01N15/14 , G01N33/553
摘要： Disclosed are an optical flow particle apparatus and method for conducting a particle light scatter-based immunoassay for simultaneously measuring the presence or amount of one or more analytes in a fluid sample which involves the use of a reagent set for each analyte including first binding molecule-coated monodisperse microspheres and second binding molecule-coated colloidal particles in which at least one of the first or second binding molecules specifically binds a respective one of the analytes. In the case where more than one analytes are detected, each monodisperse microperse microsphere of a particular reagent set has a light scatter signal resolvable from that of microspheres of any other reagent set. Changes determined in the distributions of the measured light scatter signals for individual microspheres of each of the particular reagent sets are indicative of the presence or amount of the respective analyte(s) in the sample.
摘要翻译：公开了一种用于同时测量流体样品中一种或多种分析物的存在或量的用于进行基于粒子光散射的免疫测定的光学流动颗粒装置和方法，其涉及使用每种分析物的试剂组，包括第一结合分子 - 涂覆的单分散微球和第二结合分子涂覆的胶体颗粒，其中第一或第二结合分子中的至少一个特异性结合相应的一种分析物。在检测到多于一种分析物的情况下，特定试剂组的每个单分散微粉末微球具有可从任何其它试剂组的微球分解的光散射信号。每个特定试剂组的单个微球的测量的光散射信号的分布中确定的变化指示样品中相应分析物的存在或量。

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