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1. 发明申请

US20030134282A1 Diagnosis and treatment of myocardial failure 有权
公开(公告)号：US20030134282A1
公开(公告)日：2003-07-17
申请号：US09969086
申请日：2001-10-01
申请人： University Technology Corporation
发明人： Michael R. Bristow , Leslie A. Leinwand , Wayne Minobe , Koichi Nakao , Koichiro Kinugawa
IPC分类号： C12Q001/68 , C12P019/34 , G06F019/00 , G01N033/48 , G01N033/50
CPC分类号： C12Q1/6883 , C07K14/4716 , C12Q1/6809 , C12Q2600/158
摘要： The invention provides methods of diagnosing myocardial failure in a human. The methods comprise obtaining a sample of myocardial tissue from a ventricle of the heart of the human. In one method, the expression of null-myosin heavy chain ((null-MHC), null-myosin heavy chain (null-MHC), or both in the sample is quantitated, and it is determined by statistical analysis if the expression of null-MHC, null-MHC, or both in the sample is significantly different than their expression in normal human ventricular myocardial tissue. A second method comprises quantitating the expression of null1-thyroid hormone receptor (null1-THR), null2-THR, null1-THRnullnull1-THR, or combinations thereof in the sample, and determining by statistical analysis if the expression of null1-THR, null2-THR, or null1-THRnullnull1-THR in the sample is significantly different than their expression in normal human ventricular myocardial tissue. The invention also provides kits for diagnosing myocardial failure by these methods. The invention further provides methods of treating myocardial failure in a human. One method comprises administering an effective amount of an agent that directly causes an increase in the quantity of null-MHC in the myocardial tissue of the heart. A second method comprises administering an effective amount of an agent that directly causes an increase in the quantity of null1-THR, an increase in the quantity of null1-THR, a decrease in the quantity of (null2-THR, or combinations thereof, in the myocardial tissue of the heart. Finally, the invention provides a method of quantitating the expression of a first protein relative to the expression of a second protein or to the total expression of the first and second proteins. The method comprises obtaining a sample of cells or tissue expressing the first protein and the second protein, extracting RNA from the cells or tissue, preparing cDNA from the RNA, amplifying the cDNA coding for the first and second proteins by polymerase chain reaction using primers that hybridize to cDNA coding for the first protein, the second protein or both, and quantitating the amplified PCR products.

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