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1. 发明申请

WO2008005631A3 GENES ENCODING CHAVICOL/EUGENOL SYNTHASE FROM THE CREOSOTE BUSH LARREA TRIDENTATA 审中-公开
标题翻译：基因编码CHAVICOL / EUGENOL SYNTHASE从CREOSOTE BUSH LARREA TRIDENTATA
公开(公告)号：WO2008005631A3
公开(公告)日：2009-06-11
申请号：PCT/US2007069911
申请日：2007-05-29
申请人： UNIV WASHINGTON STATE , LEWIS NORMAN G , DAVIN LAURENCE B , KIM SUNG-JIN , VASSAO DANIEL GIDDINGS , PATTEN ANN M , EICHINGER DIETMAR
发明人： LEWIS NORMAN G , DAVIN LAURENCE B , KIM SUNG-JIN , VASSAO DANIEL GIDDINGS , PATTEN ANN M , EICHINGER DIETMAR
IPC分类号： A61K36/00 , C07K14/37
CPC分类号： A01H5/02 , C12N9/0004 , C12N15/8243 , C12P7/22 , Y02E50/17
摘要： Novel methods are provided for redirecting carbon allocation in plants or cell culture from lignification to more useful and tractable materials, and to facilitate the generation of, e.g. biofuels from the remaining plant to culture biomass. Further, novel methods are provided for converting monolignols into allyl/propenyl phenols, and for chavicol/eugenol formation or production. Additional aspects relate to chavicol/eugenol synthases that convert p-coumaryl/coniferyl alcohol esters into chavicol/eugenol, and to compositions and methods using the same for producing or forming chavicol/eugenol and other derivatives in cell culture and/or genetically modified plants, and for re-engineering the composition of plant biomass. Novel methods are also provided for generation in culture or in planta of liquid/combustible allyl/propenyl phenols, and these phenolic products are utilized for (non-ethanol) biofuel/bioenergy purposes, while the remaining plant biomass facilitates the generation of other biofuels.
摘要翻译：提供了新颖的方法，用于将植物或细胞培养物中的碳分配从木质化转向更有用和易处理的材料，并且促进生成。生物燃料从剩余的植物到培养生物质。此外，提供了将单螺烯醇转化成烯丙基/丙烯基酚，以及用于切维霉素/丁子香酚形成或生产的新方法。另外的方面涉及将对香豆醇/松柏烯醇酯转化成切维霉素/丁子香酚的切维霉素/丁子香酚合成酶，以及使用该化合物在细胞培养和/或转基因植物中生产或形成辣椒素/丁子香酚和其它衍生物的组合物和方法，并重新设计植物生物量的组成。还提供了用于在液体/可燃性烯丙基/丙烯基苯酚的培养或植物中产生的新方法，并且这些酚类产物用于（非乙醇）生物燃料/生物能源目的，而剩余的植物生物质促进了其他生物燃料的产生。

2. 发明申请

WO2012064879A3 AROGENATE DEHYDRATASES AND LIGNIFICATION 审中-公开
标题翻译： AROGENATE DEHYDRATASES和LIGNIFICATION
公开(公告)号：WO2012064879A3
公开(公告)日：2012-09-20
申请号：PCT/US2011060043
申请日：2011-11-09
申请人： UNIV WASHINGTON STATE , LEWIS NORMAN G , DAVIN LAURENCE B , COREA OLIVER R A , KIM SUNG JIM
发明人： LEWIS NORMAN G , DAVIN LAURENCE B , COREA OLIVER R A , KIM SUNG JIM
IPC分类号： A01H5/00 , C12N9/88 , C12N15/29 , C12N15/60 , C12N15/82
CPC分类号： C12N15/8255 , C12N9/88
摘要： Provided are methods for decreasing carbon flow into lignin in plants, comprising reducing or eliminating, using mutagenesis and/or recombinant means, expression and/or activity of at least one chloroplast-localized arogenate dehydratase (ADT) sufficient to reduce phenylalanine (Phe) availability for metabolism into Phe-derived phenylpropanoids, wherein the amount, level or distribution of lignin is reduced relative to control plants. In particular aspects, the plant has a plurality of chloroplast-localized ADTs, and reducing or eliminating comprises reducing or eliminating expression and/or activity of at least two of the plurality of ADTs. Also provided are recombinant plants or parts or cells thereof, comprising at least one mutation, genetic alteration or transgene that reduces or eliminates the expression and/or activity of at least one chloroplast-localized ADT, wherein the amount, level or distribution of lignin is reduced relative to normal. Further provided are reduced lignin plant products.
摘要翻译：提供了减少植物中进入木质素的碳流的方法，包括使用诱变和/或重组手段减少或消除足以降低苯丙氨酸（Phe）可利用性的至少一种叶绿体定位的雄激素脱水酶（ADT）的表达和/或活性用于代谢为Phe衍生的苯丙素类，其中相对于对照植物，木质素的量，水平或分布降低。在具体方面，植物具有多个叶绿体定位的ADT，并且减少或消除包括减少或消除多个ADT中的至少两个的表达和/或活性。还提供了重组植物或其部分或细胞，其包含至少一种突变，遗传改变或转基因，其减少或消除至少一种叶绿体定位的ADT的表达和/或活性，其中木质素的量，水平或分布为相对于正常减少。还提供了减少的木质素植物产品。

3. 发明申请

WO0149833A3 RECOMBINANT PINORESINOL/LARICIRESINOL REDUCTASE, RECOMBINANT DIRIGENT PROTEIN, AND METHODS OF USE 审中-公开
标题翻译：重组胆红素/ LARICIRESINOL还原酶，重组原核蛋白及其使用方法
公开(公告)号：WO0149833A3
公开(公告)日：2002-02-14
申请号：PCT/US0035265
申请日：2000-12-22
申请人： UNIV WASHINGTON , UNIV MINNESOTA , LEWIS NORMAN G , DAVIN LAURENCE B , DINKOVA KOSTOVA ALBENA T , FUJITA MASAYUKI , GANG DAVID R , FORD JOSHUA D , SARKANEN SIMO
发明人： LEWIS NORMAN G , DAVIN LAURENCE B , DINKOVA-KOSTOVA ALBENA T , FUJITA MASAYUKI , GANG DAVID R , FORD JOSHUA D , SARKANEN SIMO
IPC分类号： C12N15/09 , C07K14/415 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/82 , C12P7/22 , C12P17/04
CPC分类号： C12N9/0004 , C07K14/415 , C12N15/8243 , C12P7/22 , C12P17/04
摘要： Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/laricinesinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteinsor pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.
摘要翻译：已经分离了多种蛋白质和pinoresinol / lariciresinol还原酶，以及编码dibigent蛋白质和pinoresinol / lariciresinol还原酶的cDNA。因此，提供了分离的DNA序列，其编码dirigent蛋白质和pinoresinol / laricinesinol还原酶的表达。在其它方面，提供可复制的重组克隆载体，其编码dirigent蛋白质或pinoresinol / lariciresinol还原酶或与至少一部分dirigent蛋白质或pinoresinol / lariciresinol还原酶DNA或RNA充分互补的碱基序列，以使其能够进行杂交。在另外的方面，提供已经用编码dirigent蛋白质或pinoresinol / lariciresinol还原酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。因此，提供了用于重组表达dirigent蛋白质和/或pinoresinol / lariciresinol还原酶的系统和方法。

4. 发明申请

WO0172978A3 ARYL PROPENAL DOUBLE BOND REDUCTASE 审中-公开
公开(公告)号：WO0172978A3
公开(公告)日：2002-05-16
申请号：PCT/US0109775
申请日：2001-03-27
申请人： UNIV WASHINGTON , KASAHARA HIROYUKI , DAVIN LAURENCE B , LEWIS NORMAN G
发明人： KASAHARA HIROYUKI , DAVIN LAURENCE B , LEWIS NORMAN G
IPC分类号： C12N15/12 , C12Q1/68 , C12N15/52 , C12N5/10 , C12N9/00
CPC分类号： C12Q1/689
摘要： In one aspect, the present invention provides isolated nucleic acid molecules that each: (a) encode an aryl propenal double bond reductase; and (b) hybridize to a nucleic acid molecule consisting of the complement of the nucleic acid sequence set forth in SEQ ID NO: 1 under defined conditions. The present invention also provides isolated aryl propenal double bond reductases. In other aspects, the present invention provides methods of enhancing or inhibiting the expression of aryl propenal double bond reductases in a plant.
摘要翻译：一方面，本发明提供分离的核酸分子，其各自：（a）编码芳基丙烯醛双键还原酶; 和（b）在确定的条件下与由SEQ ID NO：1所示的核酸序列的互补体组成的核酸分子杂交。本发明还提供了分离的芳基丙烯醛双键还原酶。在其它方面，本发明提供增强或抑制植物中芳基丙烯醛双键还原酶表达的方法。

5. 发明申请

WO0220548A9 MONOCOT SEEDS WITH INCREASED LIGNAN CONTENT 审中-公开
标题翻译： MONOCOT种子具有增加的LIGNAN内容
公开(公告)号：WO0220548A9
公开(公告)日：2002-10-17
申请号：PCT/US0127500
申请日：2001-09-04
申请人： UNIV WASHINGTON , APPLIED PHYTOLOGICS INC , LEWIS NORMAN G , DAVIN LAURENCE B , HUANG NING
发明人： LEWIS NORMAN G , DAVIN LAURENCE B , HUANG NING
IPC分类号： C07K14/415 , C12N9/02 , C12N15/82 , C07H21/04 , A01H5/00
CPC分类号： C12N9/0061 , C07K14/415 , C12N9/0004 , C12N15/8209 , C12N15/8222 , C12N15/8243
摘要： The present invention provides methods for modifying lignan content in plants by transforming plants with vectors containing a DNA sequence encoding one or more proteins integral to the phenylpropanoid pathway leading to G-lignan formation. Such coding sequences are expressed under the control of a seed tissue specific or seed developmental stage specific promoter. Expression of the DNA sequence results in a modification of the absolute and/or relative level of an intermediate metabolite leading to the production of G-lignans ( e.g. ), secoisolariciresinol diglucoside or matairesinol).
摘要翻译：本发明提供了通过用含有编码一种或多种蛋白质的DNA序列的载体转化植物来改变植物中木素素含量的方法，该蛋白质整合到导致G-木质素形成的苯丙素类途径中。这样的编码序列在种子组织特异性或种子发育阶段特异性启动子的控制下表达。 DNA序列的表达导致中间代谢物的绝对和/或相对水平的改变，导致产生G-木酚素（例如），间二异硫氰酸二氢二葡萄糖苷或马来西林（matairesinol））。

6. 发明公开

EP2046110A4 GENES ENCODING CHAVICOL/EUGENOL SYNTHASE FROM THE CREOSOTE BUSH LARREA TRIDENTATA 审中-公开
标题翻译：基因负责胡椒酚/丁香合酶的石炭酸灌木LARREA CODE三齿
公开(公告)号：EP2046110A4
公开(公告)日：2010-10-27
申请号：EP07797855
申请日：2007-05-29
申请人： UNIV WASHINGTON STATE
发明人： LEWIS NORMAN G , DAVIN LAURENCE B , KIM SUNG-JIN , VASSAO DANIEL GIDDINGS , PATTEN ANN M , EICHINGER DIETMAR
IPC分类号： C12N15/53 , A01H5/02 , C12N9/02 , C12N15/82 , C12P7/22
CPC分类号： A01H5/02 , C12N9/0004 , C12N15/8243 , C12P7/22 , Y02E50/17

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