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    • 1. 发明申请
      WO0000597A3 NON-SYMBIOTIC PLANT HEMOGLOBINS TO MAINTAIN CELL ENERGY STATUS 审中-公开
    • NON-SYMBIOTIC PLANT HEMOGLOBINS TO MAINTAIN CELL ENERGY STATUS
    • 非正常植物HEMOGLOBIN以维持细胞能量状态
    • WO0000597A3
    • 2000-03-23
    • PCT/CA9900587
    • 1999-06-24
    • UNIV MANITOBA , GUY PHILLIP , DUFF STEPHEN , XIANZHOU NIE , HILL ROBERT , DURNIN DOUGLAS , SOWA ALEKSANDER
    • GUY PHILLIP , DUFF STEPHEN , XIANZHOU NIE , HILL ROBERT , DURNIN DOUGLAS , SOWA ALEKSANDER
    • A01H5/00 , A61K35/12 , C07K14/415 , C07K14/805 , C12N1/20 , C12N15/29 , C12N15/82 , C12N15/00
    • C07K14/415 , A61K35/12 , C07K14/805 , C12N1/20 , C12N15/8261 , Y02A40/146
    • Nonsymbiotic hemoglobins are broadly present across evolution; however, the function of these proteins is unknown. Cultured maize cells have been transformed to constitutively express a barley hemoglobin gene in either the sense (HB ) or antisense (HB ) orientation. Hemoglobin protein in the transformed cell lines was correspondingly higher or lower than in wild type cells under normal atmospheric conditions. Limiting oxygen availability, by placing the cells in a nitrogen atmosphere for 12 hours, had little effect on the energy status of cells constitutively expressing hemoglobin, but had a pronounced effect on both wild type and HB cells, where ATP levels declined by 27 % and 61 % respectively. Energy charge was relatively unaffected by the treatment in HB and wild type cells, but was reduced from 0.91 to 0.73 in HB cells suggesting that the latter were incapable of maintaining their energy status under the low oxygen regime. Similar results were observed with P. aeruginosa cells transformed with an Hb expression vector. It is suggested that nonsymbiotic hemoglobins act to maintain the energy status of cells in low oxygen environments and that they accomplish this effect by promoting glycolytic flux through NADH oxidation, resulting in increased substrate level phosphorylation. Nonsymbiotic hemoglobins are likely ancestors of an early form of hemoglobin that sequestered oxygen in low oxygen environments, providing a source of oxygen to oxidize NADH to provide ATP for cell growth and development. This in turn suggests that cells containing increased levels of Hb protein will survive longer under low oxygen tension or high energy demand.
    • 非共生血红蛋白在进化中广泛存在; 然而，这些蛋白质的功能是未知的。 培养的玉米细胞已经被转化成在正义（HB +）或反义（HB））取向中组成型表达大麦血红蛋白基因。 在正常大气条件下，转化细胞系中的血红蛋白蛋白质相对较高或低于野生型细胞。 通过将细胞置于氮气氛中12小时来限制氧气供应对组成型表达血红蛋白的细胞的能量状态几乎没有影响，但对野生型和HB型细胞都有明显的作用，其中ATP水平下降 分别为27％和61％。 在HB +和野生型细胞中，能量电荷相对不受治疗的影响，但在HB 细胞中，能量电荷从0.91降低到0.73，表明后者在低氧状态下不能保持其能量状态。 用Hb表达载体转化的铜绿假单胞菌细胞观察到类似的结果。 建议非共生血红蛋白起作用以维持低氧环境中细胞的能量状态，并通过促进糖酵解通过NADH氧化来实现这一作用，从而增加底物水平的磷酸化。 非共生血红蛋白可能是早期形式的血红蛋白的祖先，其在低氧环境中螯合氧，提供氧气来氧化NADH以提供ATP用于细胞生长和发育。 这反过来表明，含有较高水平的Hb蛋白的细胞将在低氧压力或高能量需求下生存更长时间。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 5. 发明专利
      CA2331772C HEMOGLOBINS TO MAINTAIN CELL ENERGY STATUS 未知
    • HEMOGLOBINS TO MAINTAIN CELL ENERGY STATUS
    • CA2331772C
    • 2009-08-04
    • CA2331772
    • 1999-06-24
    • UNIV MANITOBA
    • SOWA ALEKSANDER , DURNIN DOUGLAS , GUY PHILLIP , HILL ROBERT , DUFF STEPHEN , NIE XIANZHOU
    • C12N15/00 , A01H5/00 , A61K35/12 , C07K14/415 , C07K14/805 , C12N1/20 , C12N15/29 , C12N15/82
    • Nonsymbiotic hemoglobins are broadly present across evolution; however, the function of these proteins is unknown. Cultured maize cells have been transformed to constitutively express a barley hemoglobin ge ne in either the sense (HB+) or antisense (HB-) orientation. Hemoglobin protein in the transformed cell lines was correspondingly higher or lower than in wild type cells under normal atmospheric conditions. Limiting oxygen availability, by placing the cells in a nitrogen atmosphere for 12 hours, had little effect on the energy status of cells constitutively expressing hemoglobin, but had a pronounced effect o n both wild type and HB- cells, where ATP levels declined by 27 % and 61 % respectively. Energy charge was relatively unaffected by the treatment in HB+ and wild type cells, but was reduced from 0.91 to 0.73 in HB- cells suggesting that the latter were incapable of maintaining their energy status under the low oxygen regime. Similar results were observed with P. aeruginosa cells transformed with an Hb expression vector. It is suggested that nonsymbiotic hemoglobins act to maintain the energy status of cells in low oxygen environments and th at they accomplish this effect by promoting glycolytic flux through NADH oxidation, resulting in increased substrate level phosphorylation. Nonsymbiotic hemoglobins are likely ancestors of an early form of hemoglobin that sequestered oxygen in low oxygen environments, providing a source of oxygen to oxidize NADH to provide ATP for cell growth and development. This in turn suggests that cells containing increased levels of Hb protein will survive longer under low oxygen tension or high energy demand.
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
    • 6. 发明专利
      CA2331772A1 NON-SYMBIOTIC PLANT HEMOGLOBINS TO MAINTAIN CELL ENERGY STATUS 未知
    • NON-SYMBIOTIC PLANT HEMOGLOBINS TO MAINTAIN CELL ENERGY STATUS
    • CA2331772A1
    • 2000-01-06
    • CA2331772
    • 1999-06-24
    • UNIV MANITOBA
    • SOWA ALEKSANDER , DUFF STEPHEN , HILL ROBERT , DURNIN DOUGLAS , GUY PHILLIP , NIE XIANZHOU
    • A01H5/00 , A61K35/12 , C07K14/415 , C07K14/805 , C12N1/20 , C12N15/29 , C12N15/82 , C12N15/00
    • Nonsymbiotic hemoglobins are broadly present across evolution; however, the function of these proteins is unknown. Cultured maize cells have been transformed to constitutively express a barley hemoglobin gene in either the sense (HB+) or antisense (HB-) orientation. Hemoglobin protein in the transformed cell lines was correspondingly higher or lower than in wild type cells under normal atmospheric conditions. Limiting oxygen availability, by placing the cells in a nitrogen atmosphere for 12 hours, had little effect on the energy status of cells constitutively expressing hemoglobin, but had a pronounced effect on both wild type and HB- cells, where ATP levels declined by 27 % and 61 % respectively. Energy charge was relatively unaffected by the treatment in HB+ and wild type cells, but was reduced from 0.91 to 0.73 in HBcells suggesting that the latter were incapable of maintaining their energy status under the low oxygen regime. Similar results were observed with P. aeruginosa cells transformed with an Hb expression vector. It is suggested that nonsymbiotic hemoglobins act to maintain the energy status of cells in low oxygen environments and that they accomplish this effect by promoting glycolytic flux through NADH oxidation, resulting in increased substrate level phosphorylation. Nonsymbiotic hemoglobins are likely ancestors of an early form of hemoglobin that sequestered oxygen in low oxygen environments, providing a source of oxygen to oxidize NADH to provide ATP for cell growth and development. This in turn suggests that cells containing increased levels of Hb protein will survive longer under low oxygen tension or high energy demand.
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
    • 7. 发明专利
      AU4595499A Nonsymbiotic plant hemoglobins to maintain cell energy status 未知
    • Nonsymbiotic plant hemoglobins to maintain cell energy status
    • AU4595499A
    • 2000-01-17
    • AU4595499
    • 1999-06-24
    • UNIV MANITOBA
    • GUY PHILLIP , DUFF STEPHEN , XIANZHOU NIE , HILL ROBERT , DURNIN DOUGLAS , SOWA ALEKSANDER
    • A01H5/00 , A61K35/12 , C07K14/415 , C07K14/805 , C12N1/20 , C12N15/29 , C12N15/82 , C12N15/00
    • Nonsymbiotic hemoglobins are broadly present across evolution; however, the function of these proteins is unknown. Cultured maize cells have been transformed to constitutively express a barley hemoglobin gene in either the sense (HB + ) or antisense (HB - ) orientation. Hemoglobin protein in the transformed cell lines was correspondingly higher or lower than in wild type cells under normal atmospheric conditions. Limiting oxygen availability, by placing the cells in a nitrogen atmosphere for 12 hours, had little effect on the energy status of cells constitutively expressing hemoglobin, but had a pronounced effect on both wild type and HB - cells, where ATP levels declined by 27% and 61% respectively. Energy charge was relatively unaffected by the treatment in HB + and wild type cells, but was reduced from 0.91 to 0.73 in HB - cells suggesting that the latter were incapable of maintaining their energy status under the low oxygen regime. Similar results were observed with P. aeruginosa cells transformed with an Hb expression vector. It is suggested that nonsymbiotic hemoglobins act to maintain the energy status of cells in low oxygen environments and that they accomplish this effect by promoting glycolytic flux through NADH oxidation, resulting in increased substrate level phosphorylation. Nonsymbiotic hemoglobins are likely ancestors of an early form of hemoglobin that sequestered oxygen in low oxygen environments, providing a source of oxygen to oxidize NADH to provide ATP for cell growth and development. This in turn suggests that cells containing increased levels of Hb protein will survive longer under low oxygen tension or high energy demand.
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
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