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    • 1. 发明申请
    • NON-SYMBIOTIC PLANT HEMOGLOBINS TO MAINTAIN CELL ENERGY STATUS
    • 非正常植物HEMOGLOBIN以维持细胞能量状态
    • WO0000597A3
    • 2000-03-23
    • PCT/CA9900587
    • 1999-06-24
    • UNIV MANITOBAGUY PHILLIPDUFF STEPHENXIANZHOU NIEHILL ROBERTDURNIN DOUGLASSOWA ALEKSANDER
    • GUY PHILLIPDUFF STEPHENXIANZHOU NIEHILL ROBERTDURNIN DOUGLASSOWA ALEKSANDER
    • A01H5/00A61K35/12C07K14/415C07K14/805C12N1/20C12N15/29C12N15/82C12N15/00
    • C07K14/415A61K35/12C07K14/805C12N1/20C12N15/8261Y02A40/146
    • Nonsymbiotic hemoglobins are broadly present across evolution; however, the function of these proteins is unknown. Cultured maize cells have been transformed to constitutively express a barley hemoglobin gene in either the sense (HB ) or antisense (HB ) orientation. Hemoglobin protein in the transformed cell lines was correspondingly higher or lower than in wild type cells under normal atmospheric conditions. Limiting oxygen availability, by placing the cells in a nitrogen atmosphere for 12 hours, had little effect on the energy status of cells constitutively expressing hemoglobin, but had a pronounced effect on both wild type and HB cells, where ATP levels declined by 27 % and 61 % respectively. Energy charge was relatively unaffected by the treatment in HB and wild type cells, but was reduced from 0.91 to 0.73 in HB cells suggesting that the latter were incapable of maintaining their energy status under the low oxygen regime. Similar results were observed with P. aeruginosa cells transformed with an Hb expression vector. It is suggested that nonsymbiotic hemoglobins act to maintain the energy status of cells in low oxygen environments and that they accomplish this effect by promoting glycolytic flux through NADH oxidation, resulting in increased substrate level phosphorylation. Nonsymbiotic hemoglobins are likely ancestors of an early form of hemoglobin that sequestered oxygen in low oxygen environments, providing a source of oxygen to oxidize NADH to provide ATP for cell growth and development. This in turn suggests that cells containing increased levels of Hb protein will survive longer under low oxygen tension or high energy demand.
    • 非共生血红蛋白在进化中广泛存在; 然而,这些蛋白质的功能是未知的。 培养的玉米细胞已经被转化成在正义(HB +)或反义(HB))取向中组成型表达大麦血红蛋白基因。 在正常大气条件下,转化细胞系中的血红蛋白蛋白质相对较高或低于野生型细胞。 通过将细胞置于氮气氛中12小时来限制氧气供应对组成型表达血红蛋白的细胞的能量状态几乎没有影响,但对野生型和HB型细胞都有明显的作用,其中ATP水平下降 分别为27%和61%。 在HB +和野生型细胞中,能量电荷相对不受治疗的影响,但在HB 细胞中,能量电荷从0.91降低到0.73,表明后者在低氧状态下不能保持其能量状态。 用Hb表达载体转化的铜绿假单胞菌细胞观察到类似的结果。 建议非共生血红蛋白起作用以维持低氧环境中细胞的能量状态,并通过促进糖酵解通过NADH氧化来实现这一作用,从而增加底物水平的磷酸化。 非共生血红蛋白可能是早期形式的血红蛋白的祖先,其在低氧环境中螯合氧,提供氧气来氧化NADH以提供ATP用于细胞生长和发育。 这反过来表明,含有较高水平的Hb蛋白的细胞将在低氧压力或高能量需求下生存更长时间。
    • 2. 发明申请
    • AUTOMATED SYSTEM FOR GERMINATION TESTING USING OPTICAL IMAGING
    • 使用光学成像进行终端测试的自动化系统
    • WO2011119403A1
    • 2011-09-29
    • PCT/US2011/028799
    • 2011-03-17
    • MONSANTO TECHNOLOGY LLCDEPPERMANN, Kevin LWHITE, Brad DVIGNAUX, NathalieGUY, Phillip
    • DEPPERMANN, Kevin LWHITE, Brad DVIGNAUX, NathalieGUY, Phillip
    • A01G9/02A01G9/14
    • A01C1/025A01G7/00
    • A system prepares plant specimens, tracks the plant specimens, captures images of the plant specimens, and evaluates growth parameters of the plant specimens in the captured images. The system prepares receptacles by placing a predetermined quantity of gel, if required by a particular test, into a receptacle and a layer of material, if required by a particular test, on top of the gel. The system separates a quantity of seeds into individual seeds and places an individual seed in each receptacle between the gel layer and the cover layer. The receptacles are then arrayed into decks and carts and subjected to controlled stress conditions and conditions conducive to germination and growth. An image capture device captures backlit images of the receptacles, and a processor analyzes the captured images for growth parameters of the plant specimens and enters those parameters into a database together with a unique identifier of the plant specimen.
    • 系统准备植物标本,跟踪植物标本,捕获植物标本的图像,并评估捕获图像中植物标本的生长参数。 如果特定测试需要,则在凝胶顶部放置预定量的凝胶,如果特定试验需要的话,该系统可以将容器放置在容器和一层材料中。 该系统将一定数量的种子分成单独的种子,并将单个种子放置在凝胶层和覆盖层之间的每个容器中。 然后将容器排列成甲板和推车,并受到受控的应激条件和有利于萌发和生长的条件。 图像捕获装置捕获插座的背光图像,并且处理器分析所捕获的图像以用于植物标本的生长参数,并将这些参数连同植物标本的唯一标识符一起输入到数据库中。
    • 6. 发明授权
    • Method and system for ranking information items for display
    • 用于显示的信息项排序的方法和系统
    • US08600837B2
    • 2013-12-03
    • US12554350
    • 2009-09-04
    • Guy Phillip William KingClark Guo
    • Guy Phillip William KingClark Guo
    • G06Q30/00
    • G06Q30/0211G06Q30/02G06Q30/0631G06Q30/0641
    • A method of displaying information items, the method comprising: determining a ranking score for each of a plurality of information items based on a history of votes received for each of the information items, the determining comprising: assigning a value to the information item for each vote received in respect of that information item, assigning a weight to each value as a function of the recency of the vote, and calculating a score for each information item, the score being calculated as a first sum across all votes of the product of the value of each vote with its assigned weight, divided by a second sum of the weights across all votes; determining, responsive to a first serve request and based on at least one criterion, at least one of the information items to be displayed at a client device; and transmitting to the client device code executable by a browser application to display the at least one information item based on the ranking score.
    • 一种显示信息项的方法,所述方法包括:基于为每个信息项接收的投票历史来确定多个信息项中的每一个的排名得分,所述确定包括:为每个信息项的信息项分配值 对该信息项目收到的投票,对每个价值分配权重作为投票的新近程度的函数,以及计算每个信息项目的得分,该分数被计算为第 每个投票的分配权重的价值除以所有投票权重的第二个总和; 响应于第一服务请求并且基于至少一个准则来确定要在客户端设备处显示的信息项中的至少一个; 以及向浏览器应用程序发送可由浏览器应用执行的代码,以基于所述排名得分来显示所述至少一个信息项。
    • 8. 发明申请
    • METHOD AND SYSTEM FOR RANKING INFORMATION ITEMS FOR DISPLAY
    • 排列显示信息项目的方法和系统
    • US20110060659A1
    • 2011-03-10
    • US12554350
    • 2009-09-04
    • Guy Phillip William KingClark Guo
    • Guy Phillip William KingClark Guo
    • G06Q30/00
    • G06Q30/0211G06Q30/02G06Q30/0631G06Q30/0641
    • A method of displaying information items, the method comprising: determining a ranking score for each of a plurality of information items based on a history of votes received for each of the information items, the determining comprising: assigning a value to the information item for each vote received in respect of that information item, assigning a weight to each value as a function of the recency of the vote, and calculating a score for each information item, the score being calculated as a first sum across all votes of the product of the value of each vote with its assigned weight, divided by a second sum of the weights across all votes; determining, responsive to a first serve request and based on at least one criterion, at least one of the information items to be displayed at a client device; and transmitting to the client device code executable by a browser application to display the at least one information item based on the ranking score.
    • 一种显示信息项的方法,所述方法包括:基于为每个信息项接收的投票历史来确定多个信息项中的每一个的排名得分,所述确定包括:为每个信息项的信息项分配值 对该信息项目收到的投票,对每个价值分配权重作为投票的新近程度的函数,以及计算每个信息项目的得分,该分数被计算为第 每个投票的分配权重的价值除以所有投票权重的第二个总和; 响应于第一服务请求并且基于至少一个准则来确定要在客户端设备处显示的信息项中的至少一个; 以及向浏览器应用程序发送可由浏览器应用执行的代码,以基于所述排名得分来显示所述至少一个信息项。