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1. 发明专利

JP2006075133A Method for producing lactic acid 有权
标题翻译：生产乳酸的方法
公开(公告)号：JP2006075133A
公开(公告)日：2006-03-23
申请号：JP2004265655
申请日：2004-09-13
申请人： Toyota Central Res & Dev Lab Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： YAMAGUCHI IKUO , SAOTOME OSAMU , KUROMIYA SHIGERU , ONISHI TORU , HOYA NORIKO , SAITO SATOSHI , MORI MAKOTO , NAKANO MITSURU , USUKI ARIMITSU , ISHIDA NOBUHIRO , TOKUHIRO TATEO , NAGAMORI EIJI , TAKAHASHI HARUO
IPC分类号： C12P7/56 , C12N1/19 , C12N15/09
CPC分类号： C12P7/56 , C12N9/0006
摘要： PROBLEM TO BE SOLVED: To provide a method for producing lactic acid in high optical purity that is not conventionally attained. SOLUTION: Reduction in optical purity of lactic acid is found as a result of the progress of the racemization reaction of lactic acid in the case of using lactic acid and glycerol. The optical purity of lactic acid after heating and concentration is kept high by reducing the amount of glycerol before heating and concentrating lactic acid. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供一种以往不能得到的高纯度的高纯度乳酸的制造方法。解决方案：使用乳酸和甘油的情况下，乳酸的外消旋化反应进行的结果，发现乳酸的光学纯度降低。加热浓缩后乳酸的光学纯度通过在加热和浓缩乳酸之前减少甘油的量而保持较高。版权所有（C）2006，JPO＆NCIPI

2. 发明专利

JP2006296377A Transformant for producing organic acid 审中-公开
标题翻译：生产有机酸的转化剂
公开(公告)号：JP2006296377A
公开(公告)日：2006-11-02
申请号：JP2005126963
申请日：2005-04-25
申请人： Toyota Central Res & Dev Lab Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： MORI TOMIKO , ISHIDA NOBUHIRO , TOKUHIRO TATEO , NAGAMORI EIJI , TAKAHASHI HARUO , ONISHI TORU , SAITO SATOSHI
IPC分类号： C12N15/09 , C12N1/19 , C12P7/56 , C12R1/865
摘要： PROBLEM TO BE SOLVED: To provide a gene expression system which exhibits a corresponding anti-gene transduction number effect or a high anti-gene transduction number effect on the fermentation production of an organic acid. SOLUTION: This transformant holding several kinds of promoters and a DNA encoding proteins functionally bound to the promoters and participating in the production of the organic acid. The DNA encoding the proteins participating in the production of the organic acid is expressed by several kinds of the promoters to give a higher expression-increasing effect than that on an expression using a single promoter. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供一种对有机酸的发酵生成具有相应的抗基因转导数量效应或高的抗基因转导数量效应的基因表达系统。解决方案：该转化体含有几种启动子和编码与启动子功能结合并参与有机酸生产的蛋白质的DNA。编码参与有机酸生产的蛋白质的DNA由几种启动子表达，以提供比使用单一启动子的表达增加效果更高的表达增加效果。版权所有（C）2007，JPO＆INPIT

3. 发明专利

JP2006288318A Promoter used under acidic condition and utilization of the same 有权
标题翻译：在酸性条件下使用的促销剂及其使用
公开(公告)号：JP2006288318A
公开(公告)日：2006-10-26
申请号：JP2005116016
申请日：2005-04-13
申请人： Toyota Central Res & Dev Lab Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： ISHIDA NOBUHIRO , TOKUHIRO TATEO , NAGAMORI EIJI , MORI TOMIKO , MATSUYAMA TAKASHI , TAKAHASHI HARUO , ONISHI TORU , SAITO SATOSHI
IPC分类号： C12N15/09 , C12N1/19 , C12P7/56
摘要： PROBLEM TO BE SOLVED: To provide a promoter used under an acidic condition. SOLUTION: This promoter of any of genes selected from a glyceraldehyde triphosphate dehydrogenase 1 gene (TDH1 gene), triose phosphate isomerase 1 gene (TPI1 gene), cell wall-associated protein 12 gene (CCW12 gene) and ribosomal protein S31 gene (RSP31 gene) or a promoter having an equivalent activity is used. By these promoters, it is possible to highly express the gene bonded functionally at the downstream of the promoter even under the acidic condition. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供在酸性条件下使用的促进剂。解决方案：选自甘油三磷酸脱氢酶1基因（TDH1基因），磷酸丙糖异构酶1基因（TPI1基因），细胞壁相关蛋白12基因（CCW12基因）和核糖体蛋白S31基因的任何基因的启动子（RSP31基因）或具有等效活性的启动子。通过这些启动子，即使在酸性条件下，也可以在启动子的下游高度表达功能性的基因。版权所有（C）2007，JPO＆INPIT

4. 发明专利

JP2005137306A Promoter in the presence of organic acid and its utilization 有权
标题翻译：有机酸存在的促销者及其利用
公开(公告)号：JP2005137306A
公开(公告)日：2005-06-02
申请号：JP2003379076
申请日：2003-11-07
申请人： Toyota Central Res & Dev Lab Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： ISHIDA NOBUHIRO , TOKUHIRO TATEO , NAGAMORI EIJI , TAKAHASHI HARUO , SAITO SATOSHI , ONISHI TORU
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/81 , C12P7/40 , C12P7/56 , C12R1/85
CPC分类号： C12N15/81
摘要： PROBLEM TO BE SOLVED: To provide a promoter utilizable in the presence of an organic acid. SOLUTION: A DNA having promoter activity of a gene selected from a high osmolality-responsive 7 gene (HOR7) of an yeast, a glyceraldehyde-3-phosphate dehydrogenase 2 gene (TDH2 gene), a heat shock protein 30 gene (HSP30 gene), a hexose transportation protein 7 gene (HXT7 gene), a thioredoxin peroxidase 1 gene (AHP1 gene) and a membrane protein 1-related gene (MRH1 gene) is used. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供可在有机酸存在下使用的促进剂。解决方案：具有启动子活性的DNA，其选自酵母的高渗透压反应性7基因（HOR7），甘油醛-3-磷酸脱氢酶2基因（TDH2基因），热休克蛋白30基因（ HSP30基因），己糖转运蛋白7基因（HXT7基因），硫氧还蛋白过氧化物酶1基因（AHP1基因）和膜蛋白1相关基因（MRH1基因）。版权所有（C）2005，JPO＆NCIPI

5. 发明专利

JP2007089466A Method for producing lactic acid 审中-公开
标题翻译：生产乳酸的方法
公开(公告)号：JP2007089466A
公开(公告)日：2007-04-12
申请号：JP2005282603
申请日：2005-09-28
申请人： Toyota Central Res & Dev Lab Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： ONISHI TORU , SAITO SATOSHI , HOYA NORIKO , ISHIDA NOBUHIRO , TOKUHIRO TATEO , NAGAMORI EIJI , TAKAHASHI HARUO
IPC分类号： C12P7/56 , C12R1/865
摘要： PROBLEM TO BE SOLVED: To provide a method for producing lactic acid at low cost and in high yield, and to provide a method for using thiamine.
SOLUTION: The method for producing lactic acid comprises culturing variant yeast Saccharomyces cerevisiae in the presence of thiamine in a concentration of 0.05 mg/L or higher. In this method, the variant yeast Saccharomyces cerevisiae is such as to be introduced into yeast free of lactic acid-producing ability in such a way as to enable a lactic acid dehydrogenase to be functioned. Besides, a method for promoting lactic acid production while suppressing ethanol production is provided in the case of Saccharomyces cerevisiae where a pyruvic acid dehydrogenase 1 is disrupted.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供低成本，高产率的乳酸的制造方法，提供使用硫胺素的方法。解决方案：生产乳酸的方法包括在硫胺素的存在下，以0.05mg / L或更高的浓度培养变种酵母酿酒酵母（Saccharomyces cerevisiae）。在该方法中，将变种酵母酿酒酵母以能够使乳酸脱氢酶发挥功能的方式引入到不含乳酸生成能力的酵母中。此外，在丙酮酸脱氢酶1被破坏的酿酒酵母的情况下，提供了抑制乙醇生成的方法。版权所有（C）2007，JPO＆INPIT

6. 发明专利

JP2004187643A Method for producing lactic acid of high optical purity 有权
标题翻译：生产高光泽酸的方法
公开(公告)号：JP2004187643A
公开(公告)日：2004-07-08
申请号：JP2002362891
申请日：2002-12-13
申请人： Toyota Central Res & Dev Lab Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： SAITO SATOSHI , SAOTOME OSAMU , ONISHI TORU , MATSUO YASUO , ISHIDA NOBUHIRO , TOKUHIRO TATEO , TAKAHASHI HARUO , HIRAI MASAKATA
IPC分类号： C12N15/09 , C12P7/56 , C12R1/865
摘要： PROBLEM TO BE SOLVED: To provide a method for producing lactic acid that is capable of giving a lactic acid of high optical purity. SOLUTION: The lactic acid is synthesized in a host with low lactic acid racemase activity. Since the racemization in the host can be inhibited, L-lactic acid or D-lactic acid of high optical purity can be synthesized. COPYRIGHT: (C)2004,JPO&NCIPI
摘要翻译：待解决的问题：提供能够产生高光学纯度的乳酸的乳酸的制造方法。解决方案：乳酸在具有低乳酸消旋酶活性的宿主中合成。由于可以抑制宿主中的外消旋化，所以可以合成高光学纯度的L-乳酸或D-乳酸。版权所有（C）2004，JPO＆NCIPI

7. 发明专利

JP2006020602A Method for producing lactic acid 有权
标题翻译：生产乳酸的方法
公开(公告)号：JP2006020602A
公开(公告)日：2006-01-26
申请号：JP2004203714
申请日：2004-07-09
申请人： Toyota Central Res & Dev Lab Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： NAGAMORI EIJI , TOKUHIRO TATEO , ISHIDA NOBUHIRO , TAKAHASHI HARUO , SAITO SATOSHI , SAOTOME OSAMU
IPC分类号： C12P7/56 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for efficiently producing lactic acid using a lactic acid-productive yeast.
SOLUTION: The method for producing lactic acid includes the step of culturing a gene-recombinant lactic acid-productive yeast having expressibly a gene encoding a protein having lactic acid dehydrogenase activity. In the above step, there is provided a step of making a culture with the oxygen transfer volumetric coefficient(hr
-1 ) ranging from 6 to 36.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供一种使用乳酸生产酵母有效生产乳酸的方法。解决方案：生产乳酸的方法包括培养具有可表达编码具有乳酸脱氢酶活性的蛋白质的基因的基因重组乳酸生产性酵母的步骤。在上述步骤中，提供了具有6至36范围内的氧转移体积系数（hr -1 ）的培养的步骤。版权所有（C）2006，JPO＆NCIPI

8. 发明专利

JP2006006271A Lactic acid-producing yeast and lactic acid producing method 审中-公开
标题翻译：酸乳酸和柠檬酸生产方法
公开(公告)号：JP2006006271A
公开(公告)日：2006-01-12
申请号：JP2004191562
申请日：2004-06-29
申请人： Toyota Central Res & Dev Lab Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： TOKUHIRO TATEO , ISHIDA NOBUHIRO , NAGAMORI EIJI , TAKAHASHI HARUO , SAITO SATOSHI , ONISHI TORU
IPC分类号： C12N1/19 , C12N15/09 , C12P7/56
摘要： PROBLEM TO BE SOLVED: To construct a system for highly expressing lactic acid, desirable for producing the lactic acid in a yeast.
SOLUTION: The yeast comprising a lactic acid producing yeast is provided, wherein pyruvate decarboxylase activity and alcohol dehydrogenase activity are each decreased in the yeast and a DNA which encodes a protein having lactate dehydrogenase activity is given to the yeast so as to be expressible. It is preferable that a pyruvate decarboxylase gene is destroyed in the organic acid-producing yeast. Further, it is preferable that an alcohol dehydrogenase gene is destroyed therein.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：为了构建高表达乳酸的系统，在酵母中生产乳酸是理想的。提供了包含产生乳酸的酵母的酵母，其中在酵母中各自降低丙酮酸脱羧酶活性和醇脱氢酶活性，并向酵母提供编码具有乳酸脱氢酶活性的蛋白质的DNA，从而表达。丙酮酸脱羧酶基因优选在有机酸生产酵母中被破坏。此外，优选醇脱氢酶基因被破坏。版权所有（C）2006，JPO＆NCIPI

9. 发明专利

JP2003334092A Method for controlling ethanol production 审中-公开
标题翻译：控制乙醇生产的方法
公开(公告)号：JP2003334092A
公开(公告)日：2003-11-25
申请号：JP2003065349
申请日：2003-03-11
申请人： Toyota Central Res & Dev Lab Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： ISHIDA NOBUHIRO , HIRAI MASAKATA , IMAEDA TAKAO , MIYAZAKI TSUTOMU , TOKUHIRO TATEO , SAITO SATOSHI , SAOTOME OSAMU , HOYA NORIKO , MATSUO YASUO
IPC分类号： C12N15/09 , C12N1/19 , C12P7/56 , C12R1/865
摘要： PROBLEM TO BE SOLVED: To provide a technique for controlling the production of ethanol in a host organism containing a pyruvate decarboxylase gene. SOLUTION: The ethanol production is controlled by using a transformant carrying a DNA encoding a foreign protein having a lactate dehydrogenase activity and exhibiting an affinity for a pyruvic acid substrate comparable or superior to the affinity for a pyruvic acid substrate of the intrinsic pyruvate decarboxylase in the host. COPYRIGHT: (C)2004,JPO

10. 发明专利

JP2012065604A Recombinant yeast and method of producing ethanol 审中-公开
标题翻译：重组酵母和生产乙醇的方法
公开(公告)号：JP2012065604A
公开(公告)日：2012-04-05
申请号：JP2010214024
申请日：2010-09-24
申请人： Kobe Univ , Toyota Motor Corp , トヨタ自動車株式会社 , 国立大学法人神戸大学
发明人： SAITO SATOSHI , KONDO AKIHIKO
IPC分类号： C12N1/19 , C12N15/09 , C12P7/10
CPC分类号： Y02E50/16
摘要： PROBLEM TO BE SOLVED: To provide a method which efficiently uses cellulose based biomass in a method of producing ethanol including a process which saccharifies cellulose based biomass using cellulase.SOLUTION: A recombinant yeast in which a β-glucosidase gene, a xylose reductase gene as a xylose metabolism related gene, and a β-xylosidase gene are introduced into the genome is disclosed, wherein the β-xylosidase gene is introduced in the form in which the expression control is carried out by the expression control region of a xylitol dehydrogenase gene, xylulokinase gene, and a hyperosmolarity response 7 (HOR7) gene. In addition, the method of producing ethanol includes a process which cultivates the recombinant yeast in a cellulose and/or hemicellulose containing medium including a cellulase formulation, and process which collects ethanol from the cellulose and/or hemicellulose containing medium.
摘要翻译：要解决的问题：提供一种在生产乙醇的方法中有效利用纤维素基生物质的方法，包括使用纤维素酶糖化纤维素基生物质的方法。解决方案：公开了将β-葡糖苷酶基因，作为木糖代谢相关基因的木糖还原酶基因和β-木糖苷酶基因引入基因组的重组酵母，其中将β-木糖苷酶基因引入通过木糖醇脱氢酶基因，木酮糖激酶基因和高渗性反应7（HOR7）基因的表达控制区进行表达控制的形式。此外，生产乙醇的方法包括在包含纤维素酶制剂的纤维素和/或含半纤维素的培养基中培养重组酵母的方法，以及从纤维素和/或含半纤维素的培养基收集乙醇的方法。版权所有（C）2012，JPO＆INPIT

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