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1. 发明授权

US5569589A Immunoassay element and process and immunoassay 失效
标题翻译：免疫测定元件和过程和免疫测定
公开(公告)号：US5569589A
公开(公告)日：1996-10-29
申请号：US163405
申请日：1993-12-07
申请人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Naofumi Hora , Yoshihiro Ashihara , Yukio Sudo , Toshihiro Mori
发明人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Naofumi Hora , Yoshihiro Ashihara , Yukio Sudo , Toshihiro Mori
IPC分类号： G01N31/22 , G01N33/542 , G01N33/543 , G01N33/53
CPC分类号： G01N33/54386 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.
摘要翻译：一种免疫测定元件，用于通过测定配体，配体与高分子化合物的连接产物和酶标记的抗体之间的反应引起的酶活性的变化来定量分析配体。免疫测定元件包括含有在酶存在下形成可扩散材料的非扩散性底物的底物层和含有用于进一步碎裂非可弥散材料的碎裂酶的试剂层。还提供了通过使用免疫测定元件定量分析样品中所含的配体的方法。

2. 发明授权

US07572578B2 Method for isolating and purifying a nucleic acid 有权
标题翻译：分离和纯化核酸的方法
公开(公告)号：US07572578B2
公开(公告)日：2009-08-11
申请号：US10568101
申请日：2004-09-08
申请人： Toshihiro Mori , Yoshihiko Makino , Rie Hando , Yumiko Takeshita , Hiroko Inomata
发明人： Toshihiro Mori , Yoshihiko Makino , Rie Hando , Yumiko Takeshita , Hiroko Inomata
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译：本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。

3. 发明申请

US20050277152A1 Method for separation and purification of nucleic acid and unit for separation and purification of nucleic acid 审中-公开
标题翻译：核酸分离纯化方法和核酸分离纯化单元
公开(公告)号：US20050277152A1
公开(公告)日：2005-12-15
申请号：US11155469
申请日：2005-06-20
申请人： Yoshihiko Makino , Toshihiro Mori
发明人： Yoshihiko Makino , Toshihiro Mori
IPC分类号： G01N30/00 , C07H21/00 , C12M1/00 , C12N15/09 , C12N15/10 , C12Q1/68 , C12M1/34
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2565/137
摘要： An object of the present invention is to provide a method for separation and purification of nucleic acid, which has excellent separating properties, high washing efficiency, and easy processability, which uses mass-producible solid phases with substantially uniform separating capacities, and wherein procedures can be automated, and a unit for separation and purification of nucleic acid suitable for implementing this method. The object of the present invention was attained by a method for separation and purification of nucleic acid comprising the steps of adsorbing nucleic acid onto a solid phase and desorbing the nucleic acid from the solid phase, wherein the solid phase comprises a base material in which a graft polymer chain having a hydrophilic group is bonded onto the surface; and a unit for separation and purification of nucleic acid which comprises, in a container having at least two openings, a solid phase of a base material in which a graft polymer chain having a hydrophilic group is bonded onto its surface.
摘要翻译：本发明的目的是提供一种分离和纯化核酸的方法，其具有优异的分离性能，高洗涤效率和易加工性，其使用具有基本上均匀分离能力的大量生产的固相，并且其中程序可以自动化，以及用于分离和纯化适用于实施该方法的核酸的单元。本发明的目的是通过核酸的分离和纯化方法来实现的，其包括将核酸吸附到固相上并从固相中解吸核酸的步骤，其中固相包括基质材料，其中具有亲水基团的接枝聚合物链被结合到表面上; 以及用于分离和纯化核酸的单元，其在具有至少两个开口的容器中包含其中具有亲水基团的接枝聚合物链键合到其表面上的基材的固相。

4. 发明授权

US07811758B2 Method and apparatus of automatically isolating and purifying nucleic acid 有权
标题翻译：自动分离和纯化核酸的方法和装置
公开(公告)号：US07811758B2
公开(公告)日：2010-10-12
申请号：US11547093
申请日：2005-03-28
申请人： Toshihiro Mori , Yoshihiko Makino
发明人： Toshihiro Mori , Yoshihiko Makino
IPC分类号： C12Q1/68 , C12M1/34 , C07H21/00
CPC分类号： C12N15/1006 , B01D15/14 , B01D15/163 , B01J20/26 , B01J20/262 , B01J20/265 , B01J20/28014 , B01J20/28033 , B01J20/28054 , C12N15/1017 , G01N30/32
摘要： A method of automatically isolating and purifying nucleic acid from a nucleic acid-containing specimen is provided, the method comprising: injecting a liquid into a cartridge for isolation and purification of a nucleic acid including at least two openings from one opening of the at least two openings, in which the cartridge includes a container having the at least two openings and containing a nucleic acid-adsorbent solid phase; passing the liquid through the nucleic acid-adsorbent solid phase by a pressure difference generated by a pressure generation means for generating a pressure difference between the inside and outside of the container; and discharging the liquid from the other opening of the container to the outside of the container by a pressure difference generated by the pressure generation means, wherein a pressure generated in the inside of the container by the pressure generation means is measured, a pressure change velocity and a pressure change acceleration are calculated on the basis of the value of the measured pressure, and the timing of completion of discharge of the liquid from the container is determined by use of a temporal change pattern of at least one of the measured pressure, the pressure change velocity and the pressure change acceleration.
摘要翻译：提供了一种从含核酸样品自动分离和纯化核酸的方法，所述方法包括：将液体注入用于分离和纯化核酸的液体，所述核酸包括至少两个开口的至少两个开口开口，其中所述盒包括具有所述至少两个开口并且包含核酸 - 吸附剂固相的容器; 通过由压力产生装置产生的压力差使液体通过核酸 - 吸附剂固相，用于产生容器内部和外部之间的压力差; 并且通过由压力产生装置产生的压力差将液体从容器的另一个开口排出到容器的外部，其中测量由压力产生装置在容器内部产生的压力，压力变化速度并且基于测量的压力的值来计算压力变化加速度，并且通过使用测量压力中的至少一个的时间变化模式来确定来自容器的液体的排出的完成时间，压力变化速度和压力变化加速度。

5. 发明申请

US20050266450A1 Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment 审中-公开
标题翻译：用于分析靶核酸片段的方法和用于分析靶核酸片段的试剂盒
公开(公告)号：US20050266450A1
公开(公告)日：2005-12-01
申请号：US11106612
申请日：2005-04-15
申请人： Yoshihiko Makino , Toshihiro Mori , Yoshihide Iwaki
发明人： Yoshihiko Makino , Toshihiro Mori , Yoshihide Iwaki
IPC分类号： C12P19/34 , C12Q1/42 , C12Q1/68 , G01N33/52
CPC分类号： G01N33/523 , C12Q1/42 , C12Q1/6816 , C12Q1/6869 , C12Q2565/625 , C12Q2565/301 , C12Q2521/543 , C12Q2533/101
摘要： An object of the present invention is to provide a method for analyzing a target nucleic acid fragment which can be simply and swiftly carried out by using a small apparatus, a kit for analyzing a target nucleic acid fragment using the method for analysis, and a dry analytical element for quantifying pyrophosphoric acid. The present invention provides a method for analyzing pyrophosphoric acid generated upon polymerase elongation reaction based on certain nucleotide sequence of a target nucleic acid, a kit for analysis for carrying out the above mentioned method for analysis, and a dry analytical element for quantifying pyrophosphoric acid.
摘要翻译：本发明的目的是提供一种用于分析目标核酸片段的方法，其可以通过使用小型装置简单快速地进行，使用该分析方法分析靶核酸片段的试剂盒和干燥的用于定量焦磷酸的分析元素。本发明提供一种基于靶核酸的某些核苷酸序列分析聚合酶延伸反应产生的焦磷酸的方法，用于进行上述分析方法的分析用试剂盒和定量焦磷酸的干燥分析元素。

6. 发明授权

US07432054B2 Method for separating and purifying a nucleic acid 有权
标题翻译：核酸分离纯化方法
公开(公告)号：US07432054B2
公开(公告)日：2008-10-07
申请号：US10621715
申请日：2003-07-18
申请人： Toshihiro Mori , Yoshihiko Makino
发明人： Toshihiro Mori , Yoshihiko Makino
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/567 , C07H21/02
CPC分类号： C12N15/1017 , C12N15/1006 , C12Q1/6806 , C12Q2565/137
摘要： An object of the present invention is to provide a method for separating and purifying a nucleic acid by adsorbing the nucleic acid in a test sample to a surface of a solid phase and desorbing the nucleic acid by washing and the like. The present invention provides a method for separating and purifying a nucleic acid having a predetermined length from a nucleic acid mixture, comprising a step of: adsorbing and desorbing a nucleic acid in the nucleic acid mixture containing nucleic acids having different lengths to and from a solid phase of an organic macromolecule having a hydroxyl group on surface thereof.
摘要翻译：本发明的目的是提供一种通过将测试样品中的核酸吸附到固相表面并通过洗涤等解吸核酸来分离和纯化核酸的方法。本发明提供了从核酸混合物中分离和纯化具有预定长度的核酸的方法，其包括以下步骤：在含有不同长度的核酸的核酸混合物中吸附和解吸核酸，在其表面具有羟基的有机高分子相。

7. 发明授权

US07682818B2 Apparatus for separating and purifying nucleic acid and method for separating and purifying nucleic acid 有权
标题翻译：用于分离和纯化核酸的装置和分离和纯化核酸的方法
公开(公告)号：US07682818B2
公开(公告)日：2010-03-23
申请号：US10808411
申请日：2004-03-25
申请人： Toshihiro Mori , Yoshihiko Makino
发明人： Toshihiro Mori , Yoshihiko Makino
IPC分类号： C12M1/34 , C12Q1/68 , C12P19/34 , G01N15/06 , C07H21/02
CPC分类号： C12N15/1003 , B01L3/0275 , B01L2200/0631 , B01L2300/0681 , B01L2400/0478 , B01L2400/0605 , G01N1/405
摘要： The present invention provides an apparatus for separating and purifying nucleic acids, which comprises: a cylindrical syringe having a leading end part in which a first opening part is formed, a base end part in which a second opening part is formed and an accommodation part between said first opening part and second opening part, the accommodation part being able to hold liquid therein; and a solid phase-holding member connected to said leading end part, a flow hole being formed at the leading end side of the solid phase-holding member; wherein a solid phase comprised of an organic polymer having a hydroxyl group on the surface thereof is accommodated in said solid phase-holding member, the solid phase being able to adsorb and desorb nucleic acids in a sample solution; and wherein a pressure sensor capable of detecting the pressure in the accommodation part is connected.
摘要翻译：本发明提供了一种分离和纯化核酸的装置，包括：圆柱形注射器，其具有形成有第一开口部的前端部，形成有第二开口部的基端部和第二开口部之间的容纳部，所述第一开口部分和第二开口部分，所述容纳部分能够将液体保持在其中; 以及固相保持构件，其连接到所述前端部，流动孔形成在所述固相保持构件的前端侧; 其中由表面具有羟基的有机聚合物构成的固相被容纳在所述固相保持部件中，所述固相能够吸附和解吸样品溶液中的核酸; 并且其中连接有能够检测所述容纳部中的压力的压力传感器。

8. 发明申请

US20080275228A1 Method and Apparatus of Automatically Isolating and Purifying Nucleic Acid 有权
标题翻译：自动分离和纯化核酸的方法和装置
公开(公告)号：US20080275228A1
公开(公告)日：2008-11-06
申请号：US11547093
申请日：2005-03-28
申请人： Toshihiro Mori , Yoshihiko Makino
发明人： Toshihiro Mori , Yoshihiko Makino
IPC分类号： C07H21/00 , C12M1/00
CPC分类号： C12N15/1006 , B01D15/14 , B01D15/163 , B01J20/26 , B01J20/262 , B01J20/265 , B01J20/28014 , B01J20/28033 , B01J20/28054 , C12N15/1017 , G01N30/32
摘要： A method of automatically isolating and purifying nucleic acid from a nucleic acid-containing specimen is provided, the method comprising: injecting a liquid into a cartridge for isolation and purification of a nucleic acid including at least two openings from one opening of the at least two openings, in which the cartridge includes a container having the at least two openings and containing a nucleic acid-adsorbent solid phase; passing the liquid through the nucleic acid-adsorbent solid phase by a pressure difference generated by a pressure generation means for generating a pressure difference between the inside and outside of the container; and discharging the liquid from the other opening of the container to the outside of the container by a pressure difference generated by the pressure generation means, wherein a pressure generated in the inside of the container by the pressure generation means is measured, a pressure change velocity and a pressure change acceleration are calculated on the basis of the value of the measured pressure, and the timing of completion of discharge of the liquid from the container is determined by use of a temporal change pattern of at least one of the measured pressure, the pressure change velocity and the pressure change acceleration.
摘要翻译：提供了一种从含核酸样品自动分离和纯化核酸的方法，所述方法包括：将液体注入用于分离和纯化核酸的液体，所述核酸包括至少两个开口的至少两个开口开口，其中所述盒包括具有所述至少两个开口并且包含核酸 - 吸附剂固相的容器; 通过由压力产生装置产生的压力差使液体通过核酸 - 吸附剂固相，用于产生容器内部和外部之间的压力差; 并且通过由压力产生装置产生的压力差将液体从容器的另一个开口排出到容器的外部，其中测量由压力产生装置在容器内部产生的压力，压力变化速度并且基于测量的压力的值来计算压力变化加速度，并且通过使用测量压力中的至少一个的时间变化模式来确定来自容器的液体的排出的完成时间，压力变化速度和压力变化加速度。

9. 发明申请

US20070221563A1 Nucleic Acid-Adsorbing Porous Membrane for Separating and Purifying Nucleic Acid and Apparatus for Separating and Purifying Nucleic Acid 审中-公开
标题翻译：用于分离和纯化核酸的核酸吸附多孔膜和用于分离和纯化核酸的装置
公开(公告)号：US20070221563A1
公开(公告)日：2007-09-27
申请号：US10576418
申请日：2004-10-20
申请人： Yoshiki Sakaino , Yoshihiko Makino , Toshihiro Mori
发明人： Yoshiki Sakaino , Yoshihiko Makino , Toshihiro Mori
IPC分类号： B01D63/00
CPC分类号： C12N15/1017 , B01L3/502 , B01L9/06 , B01L2200/0631 , B01L2300/0681 , B01L2400/0487 , G01N1/34 , G01N1/405
摘要： To provide a nucleic acid-adsorbing porous membrane which has an excellent separating ability, which provides a good washing efficiency, which permits convenient and expeditious procedures, which is adapted for automation and reduction in size, which can be mass produced with substantially identical separating capability, and which is adapted for a method of separating and purifying nucleic acids and to provide an apparatus using the same, a nucleic acid-adsorbing porous membrane for separating and purifying a nucleic acid, comprises a nucleic acid-adsorbing solid phase for use in a method for separating and purifying the nucleic acid, the solid phase adsorbing the nucleic acid, the method comprising the steps of: (1) adsorbing the nucleic acid to the solid phase by allowing a sample solution containing the nucleic acid to come into contact with the nucleic acid-adsorbing solid phase; (2) washing the solid phase by allowing a washing solution to come into contact with the solid phase, while the nucleic acid is adsorbed to the solid phase; and (3) desorbing the nucleic acid from the solid phase by allowing a recovering solution to come into contact with the solid phase.
摘要翻译：为了提供具有优异的分离能力的核酸吸附多孔膜，其提供了良好的洗涤效率，其允许方便和快速的程序，其适于自动化和尺寸减小，其可以基本相同的分离能力大量生产并且其适用于分离和纯化核酸的方法，并提供使用该方法的装置，用于分离和纯化核酸的核酸吸附多孔膜包括用于在核酸中的核酸吸附固相用于分离和纯化核酸的方法，所述固相吸附核酸，所述方法包括以下步骤：（1）通过使含有所述核酸的样品溶液与所述核酸接触来吸附所述核酸至所述固相核酸吸附固相; （2）通过使洗涤液与固相接触来洗涤固相，同时核酸被吸附到固相上; 和（3）通过使回收溶液与固相接触从固相中解吸核酸。

10. 发明授权

US07252939B2 Method for separating and purifying a nucleic acid 有权
标题翻译：核酸分离纯化方法
公开(公告)号：US07252939B2
公开(公告)日：2007-08-07
申请号：US10621412
申请日：2003-07-18
申请人： Toshihiro Mori , Yoshihiko Makino
发明人： Toshihiro Mori , Yoshihiko Makino
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/567 , C07H21/02
CPC分类号： C12N15/1006 , C12N15/1017
摘要： An object of the present invention is to provide a method for separating and purifying a nucleic acid by adsorbing the nucleic acid in a test sample to a surface of a solid phase and desorbing the nucleic acid by washing and the like. The present invention provides a method for separating and purifying RNA from a nucleic acid mixture, comprising a step of: adsorbing and desorbing a nucleic acid in the nucleic acid mixture containing RNA and DNA to and from a solid phase of an organic macromolecule.
摘要翻译：本发明的目的是提供一种通过将测试样品中的核酸吸附到固相表面并通过洗涤等解吸核酸来分离和纯化核酸的方法。本发明提供了从核酸混合物中分离和纯化RNA的方法，包括以下步骤：在含有有机高分子的固相的RNA和DNA的核酸混合物中吸附和解吸核酸。

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