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1. 发明授权

US5603898A Dry-type analytical element for immunoassay 失效
标题翻译：用于免疫测定的干式分析元件
公开(公告)号：US5603898A
公开(公告)日：1997-02-18
申请号：US993964
申请日：1992-12-17
申请人： Yoshihiro Ashihara , Yukio Sudo , Isao Nishizono , Toshikage Hiraoka , Tetsuji Tanimoto , Shigeki Kageyama
发明人： Yoshihiro Ashihara , Yukio Sudo , Isao Nishizono , Toshikage Hiraoka , Tetsuji Tanimoto , Shigeki Kageyama
IPC分类号： G01N33/543 , G01N33/58 , G01N21/00 , C07K16/00 , C12M1/14 , G01N33/53
CPC分类号： G01N33/581 , G01N33/54386
摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.
摘要翻译：一种用于免疫测定的干式分析元件，其具有至少一个用于根据酶免疫测定法测量样品中的配体的透水层，其包含（A）水溶性大分子底物和（B）与抗体结合的酶与能够作用于上述水溶性大分子底物的样品中的配体反应。上述分析元素还包含（C）在上述干式分析元件的上述透水层中作为配体或其衍生物与高分子化合物的共轭物的高分子抗原。本发明可以进行高灵敏度和简单的酶免疫测定。

2. 发明授权

US5569589A Immunoassay element and process and immunoassay 失效
标题翻译：免疫测定元件和过程和免疫测定
公开(公告)号：US5569589A
公开(公告)日：1996-10-29
申请号：US163405
申请日：1993-12-07
申请人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Naofumi Hora , Yoshihiro Ashihara , Yukio Sudo , Toshihiro Mori
发明人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Naofumi Hora , Yoshihiro Ashihara , Yukio Sudo , Toshihiro Mori
IPC分类号： G01N31/22 , G01N33/542 , G01N33/543 , G01N33/53
CPC分类号： G01N33/54386 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.
摘要翻译：一种免疫测定元件，用于通过测定配体，配体与高分子化合物的连接产物和酶标记的抗体之间的反应引起的酶活性的变化来定量分析配体。免疫测定元件包括含有在酶存在下形成可扩散材料的非扩散性底物的底物层和含有用于进一步碎裂非可弥散材料的碎裂酶的试剂层。还提供了通过使用免疫测定元件定量分析样品中所含的配体的方法。

3. 发明授权

US06589754B1 Immunoassay element and process for immunoassay 失效
标题翻译：免疫测定元件和免疫测定方法
公开(公告)号：US06589754B1
公开(公告)日：2003-07-08
申请号：US08946685
申请日：1997-10-08
申请人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
发明人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
IPC分类号： G01N3353
CPC分类号： G01N33/54386
摘要： An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.
摘要翻译：一种用于通过测定由高分子聚合物抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析大分子抗原的免疫测定元件。免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。还提供了通过使用免疫测定元件定量分析样品中的大分子抗原的方法。

4. 发明授权

US5547848A Immunoassay element containing a pulverized water-insoluble polysaccharide and process for immunoassay 失效
标题翻译：含有粉碎水不溶性多糖的免疫测定元件和免疫测定方法
公开(公告)号：US5547848A
公开(公告)日：1996-08-20
申请号：US393240
申请日：1995-02-23
申请人： Hiroshi Shinoki , Toshikage Hiraoka , Masashi Ogawa
发明人： Hiroshi Shinoki , Toshikage Hiraoka , Masashi Ogawa
IPC分类号： G01N33/542 , G01N33/543 , G01N33/558 , G01N33/535 , G01N33/548
CPC分类号： G01N33/54386 , G01N33/558
摘要： An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity. When the ligand is a low molecular weight antigen, competitive reactions between the ligand, enzyme-labelled antibody and conjugate of the antigen and high molecular weight compound are utilized. When the ligand is a macromolecular antigen, a reaction between the ligand and an enzyme-labelled antibody is utilized directly. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer for detecting the thus formed diffusible material. The non-diffusible substrate is composed of a pulverized insoluble polysaccharide. The reagent layer may further contain a fragmenting enzyme for further fragmenting the non-diffusible material. Also provided are processes for quantitatively analyzing both of low molecular weight and macromolecular antigens contained in any samples by the use of the immunoassay elements of the invention.
摘要翻译：用于通过确定酶活性的变化定量分析配体的免疫测定元件。当配体是低分子量抗原时，配体，酶标记抗体与抗原结合物和高分子量化合物之间的竞争性反应被利用。当配体是大分子抗原时，直接使用配体和酶标记的抗体之间的反应。免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和用于检测由此形成的可扩散材料的试剂层。非扩散性基材由粉碎的不溶性多糖组成。试剂层可以进一步包含用于进一步碎裂非扩散材料的碎裂酶。还提供了通过使用本发明的免疫测定元件定量分析任何样品中所含的低分子量和大分子抗原的方法。

5. 发明授权

US5093081A Dry-type analytical element for immunoassay 失效
标题翻译：用于免疫的干燥型分析元件
公开(公告)号：US5093081A
公开(公告)日：1992-03-03
申请号：US369332
申请日：1989-06-21
申请人： Yukio Sudo , Yoshihiro Ashihara , Toshikage Hiraoka , Isao Nishizono , Shigeki Kageyama , Tetsuji Tanimoto
发明人： Yukio Sudo , Yoshihiro Ashihara , Toshikage Hiraoka , Isao Nishizono , Shigeki Kageyama , Tetsuji Tanimoto
IPC分类号： G01N33/543
CPC分类号： G01N33/54386
摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-insoluble macromolecular substrate, and(B) an antibody, reacting with the ligand in the sample, conjugated with an enzyme capable of acting on the above water-insoluble macromolecular substrate.The above analytical substance further comprises,(C) a macromolecular substance which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct a highly sensitive and simple enzyme immunoassay.

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