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1. 发明申请

US20120295257A1 METHOD, MICROCHIP AND MIXED REAGENT FOR ANALYSIS OF NUCLEIC ACID BASE SEQUENCE 审中-公开
标题翻译：核酸碱基序列分析方法，微波和混合试剂
公开(公告)号：US20120295257A1
公开(公告)日：2012-11-22
申请号：US13468224
申请日：2012-05-10
申请人： Tomohiko Nakamura , Yuji Segawa , Hidetoshi Watanabe , Takanori Anaguchi
发明人： Tomohiko Nakamura , Yuji Segawa , Hidetoshi Watanabe , Takanori Anaguchi
IPC分类号： G01N25/04 , C12M1/34
CPC分类号： C12Q1/6827 , C12Q2527/107
摘要： A technology is provided for achieving high efficiency of nucleic acid amplification reaction and high precision of melting curve analysis when performing melting curve analysis in a nucleic acid amplification process of obtaining an amplification product in which the target nucleic acids are continuously arranged via a loop. A method for analysis of nucleic acid base sequence is provided, and includes: performing amplification reaction of target nucleic acid which is targeted for amplification, in presence of a probe nucleic acid that is capable of hybridizing with the target nucleic acid, to obtain an amplification product in which the target nucleic acids are continuously arranged via a loop site and employing as the probe nucleic acid, a probe nucleic acid having a melting temperature which is lower than the reaction temperature of the amplification reaction; and performing melting curve analysis of the amplification product and the probe nucleic acid.
摘要翻译：在获得扩增产物的核酸扩增过程中进行解链曲线分析时，提供核酸扩增反应的高效率和解链曲线分析的高精度的技术，其中靶核酸通过环路连续排列。提供了一种分析核酸碱基序列的方法，包括：在能够与靶核酸杂交的探针核酸的存在下，进行靶向扩增的靶核酸的扩增反应，得到扩增其中靶核酸通过环状位点连续排列并使用探针核酸的产物，具有低于扩增反应的反应温度的熔解温度的探针核酸; 并进行扩增产物和探针核酸的熔解曲线分析。

2. 发明申请

US20110312036A1 MICROCHIP FOR ISOTHERMAL NUCLEIC-ACID AMPLIFICATION REACTION, METHOD FOR PRODUCING THE SAME, AND ISOTHERMAL NUCLEIC-ACID AMPLIFICATION METHOD 审中-公开
标题翻译：用于同位素核酸放大反应的微波炉，其生产方法和等温核酸放大法
公开(公告)号：US20110312036A1
公开(公告)日：2011-12-22
申请号：US13158935
申请日：2011-06-13
申请人： Kensuke Kojima , Tasuku Yotoriyama , Tomohiko Nakamura , Hidetoshi Watanabe , Yuji Segawa
发明人： Kensuke Kojima , Tasuku Yotoriyama , Tomohiko Nakamura , Hidetoshi Watanabe , Yuji Segawa
IPC分类号： C12P19/34 , B05D3/00 , C12M1/00
CPC分类号： C12Q1/6848 , C12Q2547/107 , C12Q2549/101 , C12Q2565/629
摘要： A microchip for an isothermal nucleic-acid amplification reaction in which at least one of substances necessary for an isothermal amplification reaction of a nucleic acid is present in a reaction region functioning as a reaction field of the reaction, the at least one of the substances being covered with a thin film that melts at a temperature higher than room temperature and lower than a reaction temperature of the reaction.
摘要翻译：一种用于等温核酸扩增反应的微芯片，其中在作为反应的反应场的反应区域中存在核酸的等温扩增反应所需的至少一种物质，所述至少一种物质为覆盖有在高于室温并低于反应的反应温度的温度下熔化的薄膜。

3. 发明申请

US20120149005A1 REACTION TREATMENT DEVICE AND REACTION TREATMENT METHOD 审中-公开
标题翻译：反应处理装置和反应处理方法
公开(公告)号：US20120149005A1
公开(公告)日：2012-06-14
申请号：US13294443
申请日：2011-11-11
申请人： Kensuke Kojima , Hiroshi Katayama , Akira Shinozaki , Akira Toda , Yoshiaki Kato , Hidetoshi Watanabe , Tasuku Yotoriyama , Junji Kajihara , Toshio Watanabe , Masahiro Miyachi , Takanori Anaguchi
发明人： Kensuke Kojima , Hiroshi Katayama , Akira Shinozaki , Akira Toda , Yoshiaki Kato , Hidetoshi Watanabe , Tasuku Yotoriyama , Junji Kajihara , Toshio Watanabe , Masahiro Miyachi , Takanori Anaguchi
IPC分类号： C12Q3/00 , C12M1/38
CPC分类号： B01L7/52 , B01L3/5027 , B01L2200/147 , B01L2300/0654 , B01L2300/0829 , B01L2300/0887
摘要： Disclosed herein is a reaction treatment device including a temperature control section which includes a first temperature control section disposed at an outer peripheral edge part of a group of reaction regions and a planar second temperature control section, wherein the first temperature control section and the second temperature control section are disposed opposite to each other, with the reaction region group therebetween.
摘要翻译：本文公开了一种反应处理装置，其包括温度控制部，该温度控制部包括设置在一组反应区域的外周缘部的第一温度控制部和平面的第二温度控制部，其中，第一温度控制部和第二温度控制部分彼此相对设置，反应区域组在它们之间。

4. 发明授权

US09669407B2 Microchip 有权
公开(公告)号：US09669407B2
公开(公告)日：2017-06-06
申请号：US13292699
申请日：2011-11-09
申请人： Kensuke Kojima , Yuji Segawa , Hidetoshi Watanabe , Yoshiaki Kato , Toshio Watanabe
发明人： Kensuke Kojima , Yuji Segawa , Hidetoshi Watanabe , Yoshiaki Kato , Toshio Watanabe
IPC分类号： B01L3/00 , B01L9/00
CPC分类号： B01L3/502715 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/04 , B01L2200/0684 , B01L2200/0689 , B01L2200/18 , B01L2300/043 , B01L2300/044 , B01L2300/0609 , B01L2300/0672 , B01L2300/0816 , B01L2300/123 , B01L2400/049
摘要： Disclosed herein is a microchip that allows a sample to be introduced into a region easily and accurately, and which makes it possible to obtain high analysis accuracy. The microchip includes an airtight region into which a solution is externally introduced; and positioning means for positioning a channel for injecting the solution into the region by penetrating a substrate layer forming the region with respect to a puncture part of the region.

5. 发明申请

US20120121484A1 MICROCHIP 有权
公开(公告)号：US20120121484A1
公开(公告)日：2012-05-17
申请号：US13292699
申请日：2011-11-09
申请人： Kensuke Kojima , Yuji Segawa , Hidetoshi Watanabe , Yoshiaki Kato , Toshio Watanabe
发明人： Kensuke Kojima , Yuji Segawa , Hidetoshi Watanabe , Yoshiaki Kato , Toshio Watanabe
IPC分类号： B01L3/00 , B01L9/00
CPC分类号： B01L3/502715 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/04 , B01L2200/0684 , B01L2200/0689 , B01L2200/18 , B01L2300/043 , B01L2300/044 , B01L2300/0609 , B01L2300/0672 , B01L2300/0816 , B01L2300/123 , B01L2400/049
摘要： Disclosed herein is a microchip that allows a sample to be introduced into a region easily and accurately, and which makes it possible to obtain high analysis accuracy. The microchip includes an airtight region into which a solution is externally introduced; and positioning means for positioning a channel for injecting the solution into the region by penetrating a substrate layer forming the region with respect to a puncture part of the region.
摘要翻译：本文公开了一种微芯片，其允许将样品容易且准确地引入到区域中，并且可以获得高分析精度。微芯片包括外部引入溶液的气密区域; 以及定位装置，用于通过穿透相对于该区域的穿刺部分形成该区域的基底层来定位用于将溶液注入该区域的通道。

6. 发明授权

US09132424B2 Microchip and method of producing microchip 有权
标题翻译： Microchip和微芯片生产方法
公开(公告)号：US09132424B2
公开(公告)日：2015-09-15
申请号：US13577391
申请日：2011-02-01
申请人： Hidetoshi Watanabe , Yuji Segawa
发明人： Hidetoshi Watanabe , Yuji Segawa
IPC分类号： B01L99/00 , B01L3/00
CPC分类号： B01L3/5027 , B01L3/50273 , B01L2200/0689 , B01L2200/10 , B01L2200/141 , B01L2300/044 , B01L2300/0887 , B01L2300/12 , B01L2300/123 , B01L2400/049
摘要： A microchip is provided. The microchip (A) includes a substrate structure including a fluid channel (2) configured to contain a sample solution, wherein the fluid channel is maintained at a pressure lower than atmospheric pressure prior to injection of the sample solution into the fluid channel.
摘要翻译：提供微芯片。微芯片（A）包括衬底结构，其包括构造成容纳样品溶液的流体通道（2），其中在将样品溶液注入流体通道之前，将流体通道保持在低于大气压力的压力下。

7. 发明申请

US20120301372A1 MICROCHIP AND METHOD OF PRODUCING MICROCHIP 有权
标题翻译： MICROCHIP和MICROCHIP的生产方法
公开(公告)号：US20120301372A1
公开(公告)日：2012-11-29
申请号：US13577391
申请日：2011-02-01
申请人： Hidetoshi Watanabe , Yuji Segawa
发明人： Hidetoshi Watanabe , Yuji Segawa
IPC分类号： B01L3/00
CPC分类号： B01L3/5027 , B01L3/50273 , B01L2200/0689 , B01L2200/10 , B01L2200/141 , B01L2300/044 , B01L2300/0887 , B01L2300/12 , B01L2300/123 , B01L2400/049
摘要： A microchip is provided. The microchip (A) includes a substrate structure including a fluid channel (2) configured to contain a sample solution, wherein the fluid channel is maintained at a pressure lower than atmospheric pressure prior to injection of the sample solution into the fluid channel.
摘要翻译：提供微芯片。微芯片（A）包括衬底结构，其包括构造成容纳样品溶液的流体通道（2），其中在将样品溶液注入流体通道之前，将流体通道保持在低于大气压力的压力下。

8. 发明授权

US09493707B2 Process of preparing lamination film, lamination film and heat shield 有权
标题翻译：制备层压膜，层压膜和隔热层的工艺
公开(公告)号：US09493707B2
公开(公告)日：2016-11-15
申请号：US13048675
申请日：2011-03-15
申请人： Kazuhiro Oki , Wataru Majima , Hidetoshi Watanabe , Mitsuyoshi Ichihashi
发明人： Kazuhiro Oki , Wataru Majima , Hidetoshi Watanabe , Mitsuyoshi Ichihashi
IPC分类号： B05B5/06 , C09K19/56 , B32B37/24 , C09K19/20 , C09K19/32 , C09K19/34 , C09K19/52 , G02B5/30 , B32B38/00 , B32B38/16 , C09K19/04
CPC分类号： C09K19/56 , B32B37/24 , B32B2037/243 , B32B2038/0076 , B32B2038/168 , B32B2310/0831 , B32B2457/202 , C09K19/2007 , C09K19/32 , C09K19/3475 , C09K19/52 , C09K2019/0429 , C09K2019/0448 , C09K2019/0481 , C09K2019/328 , G02B5/3016 , Y10T428/31649
摘要： A process of preparing a lamination film comprising two or more layers of a fixed cholesteric liquid-crystal phase is disclosed. The process comprises (a) applying a coating liquid of a curable liquid crystal composition comprising a rod-like liquid crystal compound, an alignment-control agent capable of controlling an alignment of the rod-like liquid crystal compound and a solvent to a surface; (b) drying the applied curable liquid crystal composition to form a cholesteric liquid-crystal phase; (c) carrying out a curing reaction of the composition and fixing the cholesteric liquid-crystal phase, thereby to form a lower layer; and (d) repeating the steps (a) to (c) on the lower layer, thereby to form an upper layer; wherein at least a part of the alignment-control agent in the lower layer diffuses into the upper layer.
摘要翻译：公开了一种制备包括两层或多层固定胆甾型液晶相的层压膜的方法。该方法包括：（a）将包含棒状液晶化合物的可固化液晶组合物的涂布液，能够将棒状液晶化合物与溶剂对准的取向控制剂涂布到表面; （b）干燥所施加的可固化液晶组合物以形成胆甾醇型液晶相; （c）进行组合物的固化反应并固定胆甾型液晶相，从而形成下层; 和（d）在下层重复步骤（a）至（c），从而形成上层; 其中下层中的取向调节剂的至少一部分向上层扩散。

9. 发明授权

US08940251B2 Sample liquid supply device, sample liquid supply device set, and microchip set 有权
标题翻译：样品液体供应装置，样品液体供应装置和微芯片组
公开(公告)号：US08940251B2
公开(公告)日：2015-01-27
申请号：US13349874
申请日：2012-01-13
申请人： Hidetoshi Watanabe
发明人： Hidetoshi Watanabe
IPC分类号： B01L3/02
CPC分类号： B01L3/508 , A61M5/322 , B01L3/0296 , B01L3/502715 , B01L9/527 , B01L2200/0605 , B01L2200/141 , B01L2300/044 , B01L2300/0672 , G01N35/1079
摘要： A sample liquid supply device includes a container tip into which the sample liquid is introduced, a hollow needle provided at one end of the container tip such that a hollow part thereof communicates with inside of the container tip, and a sealing member that covers an opening from which the sample liquid is introduced, wherein the sealing member has a puncture-sealing property achieved by elastic deformation.
摘要翻译：样品液体供给装置包括：容器末端，其中引入样品液体;空心针，设置在容器尖端的一端，使得其中空部分与容器尖端的内部连通;以及密封构件，其覆盖开口从中引入样品液体，其中密封构件具有通过弹性变形实现的穿刺密封性。

10. 发明授权

US08333458B2 Droplet discharge head and ink jet head 有权
标题翻译：液滴喷头和喷墨头
公开(公告)号：US08333458B2
公开(公告)日：2012-12-18
申请号：US12538734
申请日：2009-08-10
申请人： Hidetoshi Watanabe
发明人： Hidetoshi Watanabe
IPC分类号： B41J2/135
CPC分类号： B41J2/15 , B41J2/14209 , B41J2/1609 , B41J2/1623 , B41J2/1626 , B41J2/1631 , B41J2/1632 , B41J2002/14217 , B41J2002/14225 , B41J2002/14306 , B41J2002/14459 , B41J2002/14491 , B41J2202/19 , B41J2202/20 , H05K3/125
摘要： A droplet discharge head is provided. The droplet discharge head includes: a discharge port through which a droplet is discharged; a liquid channel which communicates with the discharge port; and an actuator which includes a deformation layer defining a part of wall surfaces of the liquid channel and an electrode facing the liquid channel through the deformation layer, and which causes the deformation layer to deform toward the liquid channel when a drive signal is supplied to the electrode. A groove is formed on a surface of the electrode opposite to the deformation layer.
摘要翻译：提供液滴喷射头。液滴排放头包括：排出口，通过该排出口排出液滴; 与排出口连通的液体通道; 以及致动器，其包括限定所述液体通道的壁面的一部分的变形层和通过所述变形层面向所述液体通道的电极，并且当驱动信号被提供给所述变形层时，使所述变形层朝向所述液体通道变形电极。在与变形层相对的电极的表面上形成凹槽。

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