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    • 2. 发明授权
    • Side moulding for motor vehicle
    • 汽车侧面成型
    • US06910722B2
    • 2005-06-28
    • US10667404
    • 2003-09-23
    • Kazuo TakedaDaiichiro KawashimaMasao KobayashiKazutoshi Ikeda
    • Kazuo TakedaDaiichiro KawashimaMasao KobayashiKazutoshi Ikeda
    • B60R13/04B60R19/42
    • B60R13/04
    • A side moulding which is readily manufactured and mounted on an exterior side surface of a body or door of a motor vehicle, and is free from the generation of noise during the running of the motor vehicle or upon the opening and closing of the door of the motor vehicle. The side moulding includes a main body, clips for mounting the main body to the body or door of the motor vehicle, and cushion members provided in a rear surface of the main body. Each of the cushion members includes a cushion part for achieving a cushioning operation against the main body, and the body or door of the motor vehicle, and a fitting part for fitting to each of rib parts provided in the rear surface of the main body.
    • 容易制造并安装在机动车辆的车身或车门的外侧表面上的侧模具,并且在机动车辆行驶期间或者在机动车辆的门的打开和关闭时不产生噪音 摩托车。 侧面成型体包括主体,用于将主体安装到机动车辆的车身或门的夹子以及设置在主体的后表面中的缓冲构件。 每个缓冲构件包括用于实现对主体的缓冲操作的缓冲部件和机动车辆的车身或门,以及用于装配到设置在主体的后表面中的每个肋部分的装配部分。
    • 4. 发明授权
    • Disposable chip-type flow cell and flow cytometer using same
    • 一次性片式流动池和流式细胞仪使用它
    • US08951474B2
    • 2015-02-10
    • US13148271
    • 2010-02-05
    • Kazuo Takeda
    • Kazuo Takeda
    • B01L3/00G01N21/64G01N21/00G01N15/14
    • G01N15/1404G01N15/1427G01N15/1459G01N15/1484G01N21/53G01N21/6486G01N33/4833G01N33/5005G01N2015/1409G01N2015/149Y10T436/101666
    • The present invention provides an apparatus for analyzing particles in a solution including a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.
    • 本发明提供了一种用于分析溶液中的颗粒的装置,包括:单元,被配置为放置具有用于流动含有颗粒的样品溶液的流动路径的流通池; 被配置为照亮流过所述流动池的流路的样品溶液的单元; 检测从样品溶液中的颗粒产生的散射光和/或荧光的光电检测器; 以及基于由所述光检测器检测出的信号强度来分析所述粒子的单元,其中所述流通池具有形成在基板中的流路,在所述流路的侧面形成有反射面,所述反射面将所述 在流动池的流路中产生的光并且在基板的面内方向前进到流动池的表面的特定区域,并且光检测器将从指定区域射出的光检测到外部。
    • 5. 发明授权
    • Grille shutter device
    • 格栅快门装置
    • US08714290B2
    • 2014-05-06
    • US13551791
    • 2012-07-18
    • Masayuki KitashibaChiharu TotaniMinoru ShibataKazuo Takeda
    • Masayuki KitashibaChiharu TotaniMinoru ShibataKazuo Takeda
    • B60K11/08
    • B60K11/085Y02T10/88
    • A grille shutter device selectively opening and closing a grille opening in the front grille of a vehicle. The grille shutter device includes a fin, a motor, a transmission mechanism, a first switch, and a second switch. The fin has a support shaft, which is rotatably supported by the front grille. When supplied with electricity, the motor rotates its output shaft in one direction. The transmission mechanism converts the rotation of the output shaft to a swinging motion of the fin, thereby swinging the fin between a closed position, where the fin closes the grille opening, and an open position, where the fin opens the grille opening. When the fin is swung to the closed position, the first switch stops the motor. When the fin is swung to the open position, the second switch stops the motor.
    • 格栅快门装置选择性地打开和关闭车辆前格栅中的格栅开口。 格栅快门装置包括翅片,马达,传动机构,第一开关和第二开关。 翅片具有由前格栅可旋转地支撑的支撑轴。 当电力供电时,电机将其输出轴沿一个方向旋转。 传动机构将输出轴的旋转转换成翅片的摆动运动,从而使翅片在翅片关闭格栅开口的关闭位置和打开格栅开口的打开位置之间摆动。 当翅片摆动到关闭位置时,第一个开关停止电机。 当翅片摆动到打开位置时,第二个开关停止电机。
    • 6. 发明申请
    • METHOD FOR DETECTING LOW CONCENTRATIONS OF SPECIFIC CELL FROM HIGH CONCENTRATIONS OF CELL POPULATIONS, AND METHOD FOR COLLECTING AND ANALYZING DETECTED CELL
    • 用于检测细胞群体高浓度特定细胞浓度低的方法,以及收集和分析检测细胞的方法
    • US20130302828A1
    • 2013-11-14
    • US13885570
    • 2011-11-21
    • Kazuo TakedaFumie JimmaMasashi Takao
    • Kazuo TakedaFumie JimmaMasashi Takao
    • G01N1/30
    • G01N33/57492G01N1/30G01N15/1436G01N15/1459G01N21/6428G01N33/54333G01N33/574G01N2015/1006G01N2015/1477G01N2021/6439G01N2201/06113G01N2333/705G01N2333/70596
    • Conventional CTC detection methods have been problematic in that 1) there is no technique for automatically determining and counting live CTCs in a brief period of time, 2) no process has been developed for detecting, counting, and thereafter collecting and culturing live CTCs, and 3) there exists no flow cytometer that is contamination free and is capable of measuring an entire sample. Provided is a CTC detection method which comprises a pre-treatment step for concentrating and fluorescence staining CTCs, and a step for identifying and counting CTCs. The pre-treatment step includes attaching magnetic beads to EpCAM antibodies expressed by epithelial cell-derived CTCs and concentrating the CTCs through the use of a magnet, fluorescently labeling an epithelia cell surface marker of the CTCs through the use of EpCAM antibodies or 5E11 antibodies, and performing two types of nuclear staining, one being cell membrane-permeable and the other being cell membrane-impermeable. The identifying and counting step includes evaluating the respective absolute concentrations of live and dead CTCs in a volume of blood by automatically identifying CTCs by the ratio of a plurality of fluorescence signal intensities using a flow cytometer, and differentiating between and counting the live CTCs and the dead CTCs. In the cytometer, an entire liquid-feeding system that includes a flow cell can be replaced for each sample, and the total amount of a liquid sample can be measured.
    • 传统的CTC检测方法存在问题,即1)在短时间内没有自动确定和计数活CTC的技术,2)没有开发检测,计数,之后收集和培养活的CTC的过程, 3)不存在无污染的流式细胞仪,能够测量整个样品。 提供了一种CTC检测方法,其包括用于浓缩和荧光染色CTC的预处理步骤,以及用于鉴定和计数CTC的步骤。 预处理步骤包括将磁珠附着到由上皮细胞衍生的CTC表达的EpCAM抗体中,并通过使用磁体浓缩CTC,通过使用EpCAM抗体或5E11抗体荧光标记CTC的上皮细胞表面标志物, 并进行两种类型的核染色,一种是细胞膜可渗透的,另一种是细胞膜不可渗透的。 识别和计数步骤包括通过使用流式细胞仪自动识别多个荧光信号强度的比例来自动识别CTC,并且区分和计数活CTC和 死了四氯化碳。 在血细胞计数器中,可以为每个样品更换包括流通池的整个液体供给系统,并且可以测量液体样品的总量。