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1. 发明申请

WO2007098148A3 MTHODS AND ARRAYS FOR TARGET ANALYTE DETECTION AND DETERMINATION OF TARGET ANALYTE CONCENTRATION IN SOLUTION 审中-公开
标题翻译：目标分析仪检测和测定目标分析仪浓度的方法和阵列
公开(公告)号：WO2007098148A3
公开(公告)日：2008-10-16
申请号：PCT/US2007004349
申请日：2007-02-20
申请人： TUFTS COLLEGE , RISSIN DAVID M , WALT DAVID R , GORRIS HANS-HEINER
发明人： RISSIN DAVID M , WALT DAVID R , GORRIS HANS-HEINER
IPC分类号： C12Q1/00 , C12M1/00 , C12M1/36 , C12M1/38 , C12N11/00 , C12Q1/02 , C12Q1/18 , C12Q1/68 , G01N33/00 , G01N33/53 , G01N33/567
CPC分类号： G01N33/581 , C12Q1/6837 , G01N33/543 , G01N33/54346 , G01N33/54366 , G01N33/54386 , G01N2333/938 , C12Q2527/143
摘要： Arrays of single molecules and methods of producing an array of single molecules are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single molecule detection and quantitation. Biomolecules such as enzymes can be addressed at the single molecule level in order to discover function, detect binding partners or inhibitors, and/or measure rate constants.
摘要翻译：描述单分子阵列和产生单分子阵列的方法。在10个载体和50个皮升之间具有定义体积的数组使得能够进行单分子检测和定量。为了发现功能，检测结合配偶体或抑制剂和/或测量速率常数，可以在单分子水平处理生物分子如酶。

2. 发明申请

WO2011109364A2 ULTRA-SENSITIVE DETECTION OF MOLECULES OR PARTICLES USING BEADS OR OTHER CAPTURE OBJECTS 审中-公开
标题翻译：使用珠子或其他捕获物体对分子或颗粒进行超敏感检测
公开(公告)号：WO2011109364A2
公开(公告)日：2011-09-09
申请号：PCT/US2011026645
申请日：2011-03-01
申请人： QUANTERIX CORP , DUFFY DAVID C , RISSIN DAVID M , WALT DAVID R , FOURNIER DAVID , KAN CHEUK
发明人： DUFFY DAVID C , RISSIN DAVID M , WALT DAVID R , FOURNIER DAVID , KAN CHEUK
IPC分类号： G01N33/50 , C12Q1/68
CPC分类号： G01N33/54393 , G01N33/54306 , G01N33/54313
摘要： The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.
摘要翻译：本发明涉及用于检测流体样品中的分析物分子或颗粒的系统和方法，并且在某些情况下，确定流体样品中分子或颗粒的浓度的量度。本发明的方法可以包括相对于多个捕获物体固定多个分析物分子或颗粒。多个捕获对象的至少一部分可以在空间上分离成多个位置。可以至少部分地确定包含相对于捕获对象固定的分析物分子的反应容器的数量，来测量流体样品中分析物分子的浓度。在一些情况下，该测定可另外包括包括结合配体，前体标记试剂和/或酶组分的步骤。

3. 发明申请

WO2011109372A8 ULTRA-SENSITIVE DETECTION OF MOLECULES USING DUAL DETECTION METHODS 审中-公开
标题翻译：超灵敏检测分子的双重检测方法
公开(公告)号：WO2011109372A8
公开(公告)日：2011-11-10
申请号：PCT/US2011026657
申请日：2011-03-01
申请人： QUANTERIX CORP , DUFFY DAVID C , RISSIN DAVID M , WALT DAVID R , SONG LINAN , CHANG LEI
发明人： DUFFY DAVID C , RISSIN DAVID M , WALT DAVID R , SONG LINAN , CHANG LEI
IPC分类号： G01N33/543
CPC分类号： G01N33/54393 , G01N33/5306
摘要： Described herein are systems and methods for the detection of and/or determination of a measure of the concentration of analyte molecules or particles in a fluid sample. In some cases, the systems and methods employ techniques to reduce or limit the negative effects associated with non-specific binding events. Certain methods of the present invention involve associating the analyte molecules at least a first type of binding ligand and at least a second type of binding ligand, and spatially segregating the analyte molecules into a plurality of locations on a surface. The presence of an analyte molecule at or in a location may be determined by determining the presence of both the first type of binding ligand and the second type of binding ligand.
摘要翻译：本文描述了用于检测和/或确定流体样品中的分析物分子或颗粒浓度的量度的系统和方法。在一些情况下，系统和方法采用技术来减少或限制与非特异性结合事件相关的负面影响。本发明的某些方法涉及将分析物分子与至少第一类型的结合配体和至少第二类型的结合配体缔合，并将分析物分子空间分离成表面上的多个位置。可以通过确定第一类型结合配体和第二类型结合配体两者的存在来确定分析物分子在位置处或位置处的存在。

4. 发明申请

WO2010039180A2 HIGH SENSITIVITY DETERMINATION OF THE CONCENTRATION OF ANALYTE MOLECULES OR PARTICLES IN A FLUID SAMPLE 审中-公开
标题翻译：高灵敏度测定流体样品中分析物分子或颗粒的浓度
公开(公告)号：WO2010039180A2
公开(公告)日：2010-04-08
申请号：PCT/US2009005250
申请日：2009-09-22
申请人： QUANTERIX CORP , DUFFY DAVID C , RISSIN DAVID M , SONG LINAN , WALT DAVID R
发明人： DUFFY DAVID C , RISSIN DAVID M , SONG LINAN , WALT DAVID R
IPC分类号： C12Q1/68 , G01N33/543 , G01N33/58
CPC分类号： G01N33/54366
摘要： The present invention relates to methods, systems, and kits for detecting, quantifying and/or analyzing a fluid sample comprising molecules or particles at low concentration. In certain embodiments, the methods for detection and/or quantifying analyte molecules in a sample comprise capturing a plurality of analyte molecules on a substrate (e.g., an array comprising a plurality of reaction vessels). The substrate may then be exposed to additional reaction components such as at least one binding ligand. The substrate may additionally be exposed to a precursor labeling agent molecule, wherein the precursor labeling agent molecule, in some cases, is converted to a labeling agent molecule, which may be detected, either directly or indirectly, which determination may be related to the presence of and/or may be employed to quantify the analyte molecules. Although the various aspects of the present invention may use a number of different assay formats, in one embodiment, the assays are conducted in a plurality of reaction vessels defined, at least in part, by the distal ends of fiber optic strands.
摘要翻译：本发明涉及用于检测，定量和/或分析包含低浓度分子或颗粒的流体样品的方法，系统和试剂盒。在某些实施方案中，用于检测和/或定量样品中分析物分子的方法包括在基底（例如，包含多个反应容器的阵列）上捕获多个分析物分子。然后可将底物暴露于另外的反应组分如至少一种结合配体。所述底物可另外暴露于前体标记剂分子，其中所述前体标记剂分子在一些情况下被转化成标记剂分子，其可被直接或间接检测到，所述测定可与存在和/或可以用于量化分析物分子。尽管本发明的各个方面可以使用许多不同的测定形式，但在一个实施方案中，测定是在多个反应容器中进行的，所述反应容器至少部分地由光纤束的远端限定。

5. 发明申请

WO2008048698A3 CHEMICAL SWITCHES FOR DETECTING REACTIVE CHEMICAL AGENTS 审中-公开
标题翻译：用于检测反应性化学试剂的化学开关
公开(公告)号：WO2008048698A3
公开(公告)日：2008-07-24
申请号：PCT/US2007062568
申请日：2007-02-22
申请人： TUFTS COLLEGE , WALT DAVID R , BENCIC-NAGALE SANDRA
发明人： WALT DAVID R , BENCIC-NAGALE SANDRA
IPC分类号： G01N21/64
CPC分类号： G01N33/0057 , C07D219/06 , G01N21/643 , Y10T436/16 , Y10T436/163333
摘要： Certain embodiments of the present invention relate to the preparation of microbeads that exhibit a "turn on" fluorescence response within seconds of exposure to an analyte vapor (e.g., a chemical warfare agent or a reactive stimulant). This sensing approach is modeled after the mechanism for inhibition of acetylcholinesterase enzyme activity, and utilizes a specific and irreversible reaction between phosphonyl halides and a fluorescent indicator. The present invention also relates to a sensor and a method for sensing an analyte through detection of changes in the fluorescing properties of the inventive microbeads.
摘要翻译：本发明的某些实施方案涉及在暴露于分析物蒸汽（例如化学战剂或反应兴奋剂）的几秒钟内显示出“开启”荧光响应的微珠的制备。该感测方法在抑制乙酰胆碱酯酶活性的机制之后被建模，并利用膦酰卤与荧光指示剂之间的特异性和不可逆反应。本发明还涉及通过检测本发明微珠的荧光特性变化来检测分析物的传感器和方法。

6. 发明申请

WO0169245A3 CROSS-REACTIVE SENSORS 审中-公开
标题翻译：交叉传感器
公开(公告)号：WO0169245A3
公开(公告)日：2002-05-02
申请号：PCT/US0108126
申请日：2001-03-14
申请人： TUFTS COLLEGE , WALT DAVID R , SCHAUER CAROLINE L , STEEMERS FRANK J
发明人： WALT DAVID R , SCHAUER CAROLINE L , STEEMERS FRANK J
IPC分类号： C12Q1/37 , C12Q1/44 , G01N33/50 , C12Q1/00 , C12Q1/68 , G01N33/543 , G01N33/566
CPC分类号： C12Q1/44 , C12Q1/37
摘要： The present invention provides a novel cross-reactive sensor system utilizing cross-reactive recognition elements. In the inventive system, each of said one or more cross-reactive recognition elements is capable of interacting with more than one species of liquid analyte of interest, whereby each of said one or more cross-reactive recognition elements reacts in a different manner with each of said one or more species of liquid analytes of interest to produce a detectable agent of each analyte of interest, whereby said detectable agent is analyzed and the information is processed for data acquisition and interpretation. In certain preferred embodiments, the detectable agent and/or change is detected directly, while in certain other preferred embodiments, the detectable agent and/or change is detected with the help of a transducing agent capable of relaying information about each detectable agent generated for each of said species of liquid analyte of interest, whereby said information is processed for data acquisition and interpretation. The present invention also provides method for the analysis of analytes comprising contacting one or more analytes with the inventive system described above.
摘要翻译：本发明提供一种利用交叉反应识别元件的新型交互反应传感器系统。在本发明的系统中，所述一个或多个交叉反应性识别元件中的每一个能够与多于一种的感兴趣的液体分析物相互作用，由此所述一个或多个交叉反应性识别元件中的每一个以不同的方式与每个的所述一种或多种感兴趣的液体分析物种类以产生感兴趣的每种分析物的可检测试剂，由此分析所述可检测试剂，并且对所述信息进行处理以进行数据采集和解释。在某些优选的实施方案中，直接检测可检测的试剂和/或变化，而在某些其它优选的实施方案中，可以借助于能够中继关于每个可产生的每个可检测试剂的信息的转导剂来检测可检测的试剂和/或变化的所述物质的感兴趣的液体分析物，由此所述信息被处理用于数据采集和解释。本发明还提供了用于分析分析物的方法，包括使一种或多种分析物与上述本发明的系统接触。

7. 发明申请

WO0228530A2 SELF-ENCODING SENSOR WITH MICROSPHERES 审中-公开
标题翻译：具有微结构的自编码传感器
公开(公告)号：WO0228530A2
公开(公告)日：2002-04-11
申请号：PCT/US0131581
申请日：2001-10-09
申请人： TUFTS COLLEGE , WALT DAVID R
发明人： WALT DAVID R
IPC分类号： C40B60/14 , G01N21/77 , B01L
CPC分类号： G01N21/7703 , B01J2219/00317 , B01J2219/005 , B01J2219/00524 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00702 , C40B60/14
摘要： A microsphere-based analytic chemistry system is disclosed in which self-encoding microspheres having distinct characteristic optical response signatures to specific target analytes may be mixed together while the ability is retained to identify the sensor type and location of each sensor in a random dispersion of large numbers of such sensors in a sensor array using an optically interrogatable encoding scheme. An optical fiber bundle sensor is also disclosed in which individual microsphere sensors are disposed in microwells at a distal end of the fiber bundle and are optically coupled to discrete fibers or groups of fibers within the bundle. The identities of the individual sensors in the array are self-encoded by exposing the array to a reference analyte while illuminating the array with excitation light energy. A single sensor array may carry thousands of discrete sensing elements whose combined signal provides for substantial improvements in sensor detection limits, response times and signal-to-noise ratios.
摘要翻译：公开了一种基于微球的分析化学系统，其中具有针对特定目标分析物的不同特征光学响应特征的自编码微球可以混合在一起，同时保留能力以识别大尺度随机色散中的每个传感器的传感器类型和位置使用光学可询问编码方案的传感器阵列中的这种传感器的数量。还公开了一种光纤束传感器，其中单个微球传感器设置在纤维束的远端处的微孔中，并且光束耦合到束内的离散纤维或纤维组。阵列中各个传感器的身份通过将阵列暴露于参考分析物而进行自编码，同时用激发光能照射阵列。单个传感器阵列可携带数千个离散感测元件，其组合信号提供传感器检测限，响应时间和信噪比方面的实质性改进。

8. 发明申请

WO9850782A3 FIBER OPTIC BIOSENSOR FOR SELECTIVELY DETECTING OLIGONUCLEOTIDE SPECIES IN A MIXED FLUID SAMPLE 审中-公开
标题翻译：用于在混合液体样品中选择性检测寡核苷酸物种的光纤生物传感器
公开(公告)号：WO9850782A3
公开(公告)日：1999-02-04
申请号：PCT/US9809163
申请日：1998-05-05
申请人： TUFTS COLLEGE , WALT DAVID R , HEALEY BRIAN G , FERGUSON JANE F
发明人： WALT DAVID R , HEALEY BRIAN G , FERGUSON JANE F
IPC分类号： G01N21/77 , G01N21/64
CPC分类号： G01N21/7703 , G01N2201/0833 , Y10S977/924
摘要： The present invention provides biosensors, apparatus and methods for selectively detecting at least one complementary oligonucleotide target specie in a fluid sample containing a mixture of different oligonucleotide fragments. One preferred embodiment of the biosensor is as a unitary fiber optic array having an in-situ hybridization zone comprising not less than one specie of single stranded oligonucleotide disposed as individual deposits in aligned organization upon multiple strand end faces at differing spatial positions on the distal array end surface. In this manner, a collective of deployed, single specie, multiple fixed probes are presented for selective in-situ hybridization on-demand with at least one mobile complementary target specie ultimately bearing a joined identifying label. The biosensor provides for optical detection of in-situ hybridization on the distal end surface via the presence of the concomitantly disposed joined identifying label at the differing spatial positions.

9. 发明申请

WO0016101A9 TARGET ANALYTE SENSORS UTILIZING MICROSPHERES 审中-公开
标题翻译：目标分析物传感器利用微球
公开(公告)号：WO0016101A9
公开(公告)日：2000-06-08
申请号：PCT/US9920914
申请日：1999-09-10
申请人： TUFTS COLLEGE , WALT DAVID R , MICHAEL KARRI L
发明人： WALT DAVID R , MICHAEL KARRI L
IPC分类号： G01N21/64 , B01J19/00 , C12M1/40 , C12Q1/00 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B60/14 , G01N1/28 , G01N15/14 , G01N21/77 , G01N33/15 , G01N33/50 , G01N33/53 , G01N33/532 , G01N33/543 , G01N33/566 , G01N33/58 , G01N35/00 , G01N37/00
CPC分类号： G01N33/54346 , B01J19/0046 , B01J2219/00317 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B60/14 , G01N15/1456 , G01N15/1459 , G01N15/1468 , G01N21/6428 , G01N21/6452 , G01N21/7703 , G01N33/543 , G01N2015/1438 , G01N2015/1465 , G01N2035/0097 , Y10S359/90 , Y10S435/808
摘要： A microsphere-based analytic chemistry system and method for making the same is disclosed in which microspheres or particles carrying bioactive agents may be combined randomly or in ordered fashion and dispersed on a substrate to form an array while maintaining the ability to identify the location of bioactive agents and particles within the array using an optically interrogatable, optical signature encoding scheme. A wide variety of modified substrates may be employed which provide either discrete or non-discrete sites for accomodating the microspheres in either random or patterned distributions. The substrates may be constructed from a variety of materials to form either two-dimensional or three-dimensional configurations. In a preferred embodiment, a modified fiber optic bundle or array is employed as a substrate to produce a high density array. The disclosed system and method have utility for detecting target analytes and screening large libraries of bioactive agent.
摘要翻译：公开了一种基于微球的分析化学系统及其制造方法，其中携带生物活性剂的微球体或颗粒可以随机或有序方式组合并分散在基底上形成阵列，同时保持识别生物活性位置的能力使用光学可询问的光学签名编码方案在阵列内的代理和粒子。可以使用各种各样的修饰底物，它们提供分散或非分散的位点，以随机或图案分布方式容纳微球体。衬底可以由多种材料构成以形成二维或三维结构。在一个优选实施例中，改进的光纤束或阵列被用作基底以产生高密度阵列。所公开的系统和方法可用于检测目标分析物并筛选生物活性剂的大型文库。

10. 发明申请

WO0013004A2 COMBINATORIAL POLYMER SYNTHESIS OF SENSORS FOR POLYMER-BASED SENSOR ARRAYS 审中-公开
公开(公告)号：WO0013004A2
公开(公告)日：2000-03-09
申请号：PCT/US9919624
申请日：1999-08-26
申请人： TUFTS COLLEGE , WALT DAVID R , DICKINSON TODD A
发明人： WALT DAVID R , DICKINSON TODD A
IPC分类号： B01J19/00 , C40B40/14 , C40B70/00 , G01N21/77 , G01N21/64
CPC分类号： G01N21/7703 , B01J19/0046 , B01J2219/005 , B01J2219/00513 , B01J2219/00572 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00704 , B01J2219/00707 , B01J2219/00722 , C40B40/14 , C40B70/00 , G01N2201/0833
摘要： A combinatorial synthesis method for fabricating unique families of discrete copolymer sensors and copolymer gradient sensors is provided. The method employs combinatorial copolymer synthesis of discrete monomer or oligomer combinations as well as spatially-varying combinations for generating large numbers of analyte-discriminating sensors from a limited selection of initial monomer and oligomer compositions. The method can be applied to either analyte-specific sensors or sensor arrays or semi-selective sensors and cross-reactive sensor arrays which employ virtually any known physicochemical transduction mechanism for detecting analytes. Since the analyte response characteristics of such copolymer sensors are not limited to a linear proportional ratio of the monomer or oligomer combinations employed, the resulting copolymer sensors provide for increased diversity in sensor and sensor array response characteristics for discriminating between a variety of materials and for detecting and identifying analytes in fluid samples.

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