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1. 发明申请

WO1998024930A1 CHAIN-TERMINATION TYPE NUCLEIC ACID SEQUENCING METHOD INCLUDING 2'-DEOXYURIDINE-5'-TRIPHOSPHATE 审中-公开
标题翻译：包含2'-脱氧-5,9-二磷酸的链终止型核酸序列方法
公开(公告)号：WO1998024930A1
公开(公告)日：1998-06-11
申请号：PCT/US1997020443
申请日：1997-11-12
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , LEE, Linda, G. , ROSENBLUM, Barnett, B.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6848 , C12Q1/6869 , Y10S435/81
摘要： A chain-termination type DNA sequencing method is disclosed wherein deoxynucleotide 2'-deoxythymidine-5'-triphosphate is replaced by 2'-deoxyuridine-5'-triphosphate, or analogs thereof. Kits for performing the method are also provided.
摘要翻译：公开了一种链终止型DNA测序方法，其中脱氧核苷酸2'-脱氧胸苷-5'-三磷酸被2'-脱氧尿苷-5'-三磷酸或其类似物代替。还提供了用于执行该方法的套件。

2. 发明申请

WO1998006732A1 PROPARGYLETHOXYAMINO NUCLEOTIDES 审中-公开
标题翻译：丙基胆碱胺核苷酸
公开(公告)号：WO1998006732A1
公开(公告)日：1998-02-19
申请号：PCT/US1997011553
申请日：1997-08-13
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , KHAN, Shaheer, H. , MENCHEN, Steven, M. , ROSENBLUM, Barnett, B.
IPC分类号： C07H19/04
CPC分类号： C07H21/00 , C07H19/04 , C07H21/04 , C12Q1/686
摘要： Propargylethoxyamino nucleosides are disclosed having structure (I), wherein R1 and R2 are -H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is -H or -OH; W2 is -OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position; and W3 is -PO4, -P2O7, -P3O10, phosphate analog, or -OH. Additionally, a primer extension method is provided employing the above propargylethoxyamino nucleosides.
摘要翻译：公开了具有结构（I）的丙炔乙氧基氨基核苷，其中R1和R2是-H，低级烷基或标记; B是7-脱氮嘌呤，嘌呤或嘧啶核苷碱基; W1是-H或-OH; W2是-OH或使核苷不能在3'-位形成磷酸二酯键的部分; W3是-PO 4，-P 2 O 7，-P 3 O 10，磷酸酯类似物或-OH。另外，使用上述炔丙氧基氨基核苷提供引物延伸方法。

3. 发明申请

WO1997049769A1 4,7-DICHLORORHODAMINE DYES 审中-公开
标题翻译： 4,7-二氯丹染色体
公开(公告)号：WO1997049769A1
公开(公告)日：1997-12-31
申请号：PCT/US1997008797
申请日：1997-05-21
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , LEE, Linda , BENSON, Scott, C. , ROSENBLUM, Barnett, B. , SPUNGEON, Sandra, L.
IPC分类号： C09B11/24
CPC分类号： C07H21/00 , C09B11/24
摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having structure formula (I) wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; Y1-Y4 are hydrogen or lower alkyl, or, when taken together, Y1 and R2 is propano and Y2 and R1 is propano, or, when taken together, Y3 and R3 is propano and Y4 and R4 is propano; and X1-X3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, -CH2OH, and a linking group. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.
摘要翻译：公开了一类用作荧光染料的4,7-二氯罗丹明化合物，其具有结构式（I），其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯，低级炔，磺酸酯，砜，氨基，酰氨基，腈，低级烷氧基，连接基团或它们的组合，或者当R 1和R 6一起时为苯并，或者合并时，R 4和R 5为苯并; Y1-Y4为氢或低级烷基，当Y1和R2为丙酰基时，Y 1和R 2为丙酰基，或者当Y 3和R 3为丙酰基时，Y 4和R 4为丙酰基; 和分别选择的X1-X3选自氢，氯，氟，低级烷基，羧酸酯，磺酸，-CH 2 OH和连接基团。另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

4. 发明公开

EP0915901A1 PROPARGYLETHOXYAMINO NUCLEOTIDES 失效转让
标题翻译：丙基氨基甲酰胺核糖核酸
公开(公告)号：EP0915901A1
公开(公告)日：1999-05-19
申请号：EP97937953.0
申请日：1997-08-13
申请人： THE PERKIN-ELMER CORPORATION
发明人： KHAN, Shaheer, H. , MENCHEN, Steven, M. , ROSENBLUM, Barnett, B.
IPC分类号： C12N15 , C07H19 , C07H21 , C12Q1
CPC分类号： C07H21/00 , C07H19/04 , C07H21/04 , C12Q1/686
摘要： Propargylethoxyamino nucleosides are disclosed having structure (I), wherein R1 and R2 are -H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is -H or -OH; W2 is -OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position; and W3 is -PO4, -P2O7, -P3O10, phosphate analog, or -OH. Additionally, a primer extension method is provided employing the above propargylethoxyamino nucleosides.

5. 发明公开

EP0889973A1 CHAIN-TERMINATION TYPE NUCLEIC ACID SEQUENCING METHOD INCLUDING 2'-DEOXYURIDINE-5'-TRIPHOSPHATE 有权转让
标题翻译：链终止核酸测序方法用2'-脱氧尿苷-5'-三磷酸
公开(公告)号：EP0889973A1
公开(公告)日：1999-01-13
申请号：EP97947422.0
申请日：1997-11-12
申请人： THE PERKIN-ELMER CORPORATION
发明人： LEE, Linda, G. , ROSENBLUM, Barnett, B.
IPC分类号： C12N15 , C12Q1
CPC分类号： C12Q1/6848 , C12Q1/6869 , Y10S435/81
摘要： A chain-termination type DNA sequencing method is disclosed wherein deoxynucleotide 2'-deoxythymidine-5'-triphosphate is replaced by 2'-deoxyuridine-5'-triphosphate, or analogs thereof. Kits for performing the method are also provided.

6. 发明授权

EP0915935B1 4,7-DICHLORORHODAMINE DYES 有权转让
标题翻译： 4,7- DICHLORRHODAMINFARBSTOFFE
公开(公告)号：EP0915935B1
公开(公告)日：2001-03-07
申请号：EP97928653.1
申请日：1997-05-21
申请人： The Perkin-Elmer Corporation
发明人： LEE, Linda , BENSON, Scott, C. , ROSENBLUM, Barnett, B. , SPUNGEON, Sandra, L.
IPC分类号： C09B11/24 , C07H21/00
CPC分类号： C07H21/00 , C09B11/24
摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having structure formula (I) wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; Y1-Y4 are hydrogen or lower alkyl, or, when taken together, Y1 and R2 is propano and Y2 and R1 is propano, or, when taken together, Y3 and R3 is propano and Y4 and R4 is propano; and X1-X3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, -CH2OH, and a linking group. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

7. 发明公开

EP0923597A1 SUBSTITUTED PROPARGYLETHOXYAMIDO NUCLEOSIDES 失效
标题翻译：取代的丙酰基乙酰氨基核苷
公开(公告)号：EP0923597A1
公开(公告)日：1999-06-23
申请号：EP98914275.0
申请日：1998-03-23
申请人： THE PERKIN-ELMER CORPORATION
发明人： KHAN, Shaheer, H. , MENCHEN, Steven, M. , ROSENBLUM, Barnett, B.
IPC分类号： C12N15 , C07H19 , C07H21 , C12Q1
CPC分类号： C07H21/00 , C07H19/04 , C12Q1/6869 , Y02P20/55
摘要： Substituted propargylethoxyamido nucleosides are disclosed having structure (I) wherein X is selected from the group consisting of amino alkanoic acid, alkylamino benzoic acid, α-amino acid, and 4-amino-2-butynoic acid. R1 and R2 taken separately are selected from the group consisting of -H, lower alkyl, protecting group, and label; R3 is selected from the group consisting of -H and lower alkyl. B is a 7-deazapurine, purine, or pyrimidine nucleoside base. When B is purine or 7-deazapurine, the sugar moiety is attached at the N9-position of the purine or deazapurine, and when B is pyrimidine, the sugar moiety is attached at the N1-position of the pyrimidine. When B is a purine, the adjacent triple-bonded carbon is attached to the 8-position of the purine, when B is 7-deazapurine, the adjacent triple-bonded carbon is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, the adjacent triple-bonded carbon is attached to the 5-position of the pyrimidine. W¿1? is selected from the group consisting of -H and -OH. W2 is -OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position. W3 is selected from the group consisting of -PO4, -P2O7, -P3O10, phosphate analog, and -OH. Additionally, a primer extension method is provided employing the above X-substituted propargylethoxyamido nucleosides, and polynucleotides including the above X-substituted propargylethoxyamido nucleosides is provided.
摘要翻译：公开了具有结构（I）的取代的炔丙基乙氧基酰胺核苷，其中X选自氨基链烷酸，烷基氨基苯甲酸，α-氨基酸和4-氨基-2-丁炔酸。 R1和R2分别选自-H，低级烷基，保护基和标记; R3选自-H和低级烷基。 B是7-脱氮嘌呤，嘌呤或嘧啶核苷碱基。当B是嘌呤或7-脱氮嘌呤时，糖部分连接在嘌呤或脱氮嘌呤的N9-位置，并且当B是嘧啶时，糖部分连接在嘧啶的N1位。当B是嘌呤时，相邻的三键碳连接到嘌呤的8-位，当B是7-脱氮嘌呤时，相邻的三键碳连接到7-脱氮嘌呤的7-位，并且当B是嘧啶时，相邻的三键碳连接到嘧啶的5-位。 W¿1？选自-H和-OH。 W2是-OH或使得核苷不能在3'-位形成磷酸二酯键的部分。 W3选自-PO4，-P2O7，-P3O10，磷酸盐类似物和-OH。此外，提供了使用上述X取代的炔丙基乙氧基酰胺基核苷的引物延伸方法，并且提供了包含上述X取代的炔丙基乙氧基酰胺核苷的多核苷酸。

8. 发明授权

EP0889973B1 CHAIN-TERMINATION TYPE NUCLEIC ACID SEQUENCING METHOD INCLUDING 2'-DEOXYURIDINE-5'-TRIPHOSPHATE 有权转让
标题翻译：链终止测序法核酸与-2'-脱氧尿苷-5'-三磷酸
公开(公告)号：EP0889973B1
公开(公告)日：2001-02-07
申请号：EP97947422.8
申请日：1997-11-12
申请人： THE PERKIN-ELMER CORPORATION
发明人： LEE, Linda, G. , ROSENBLUM, Barnett, B.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6869 , Y10S435/81
摘要： A chain-termination type DNA sequencing method is disclosed wherein deoxynucleotide 2'-deoxythymidine-5'-triphosphate is replaced by 2'-deoxyuridine-5'-triphosphate, or analogs thereof. Kits for performing the method are also provided.

9. 发明授权

EP0915901B1 PROPARGYLETHOXYAMINO NUCLEOTIDES 失效转让
标题翻译：丙酰氨基核糖核苷酸
公开(公告)号：EP0915901B1
公开(公告)日：2000-10-11
申请号：EP97937953.4
申请日：1997-08-13
申请人： THE PERKIN-ELMER CORPORATION
发明人： KHAN, Shaheer, H. , MENCHEN, Steven, M. , ROSENBLUM, Barnett, B.
IPC分类号： C07H19/04 , C07H21/00 , C12Q1/68
CPC分类号： C07H21/00 , C07H19/04 , C07H21/04 , C12Q1/686

10. 发明公开

EP0915935A1 4,7-DICHLORORHODAMINE DYES 有权转让
标题翻译： 4,7-二氯
公开(公告)号：EP0915935A1
公开(公告)日：1999-05-19
申请号：EP97928653.0
申请日：1997-05-21
申请人： The Perkin-Elmer Corporation
发明人： LEE, Linda , BENSON, Scott, C. , ROSENBLUM, Barnett, B. , SPUNGEON, Sandra, L.
IPC分类号： C12N15 , C07D311 , C07D405 , C07D491 , C07F9 , C07H19 , C07H21 , C09B11 , C09K11 , C12P19 , C12Q1 , G01N21 , G01N27 , G01N33
CPC分类号： C07H21/00 , C09B11/24
摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having structure formula (I) wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; Y1-Y4 are hydrogen or lower alkyl, or, when taken together, Y1 and R2 is propano and Y2 and R1 is propano, or, when taken together, Y3 and R3 is propano and Y4 and R4 is propano; and X1-X3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, -CH2OH, and a linking group. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

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