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1. 发明申请

WO1998006732A1 PROPARGYLETHOXYAMINO NUCLEOTIDES 审中-公开
标题翻译：丙基胆碱胺核苷酸
公开(公告)号：WO1998006732A1
公开(公告)日：1998-02-19
申请号：PCT/US1997011553
申请日：1997-08-13
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , KHAN, Shaheer, H. , MENCHEN, Steven, M. , ROSENBLUM, Barnett, B.
IPC分类号： C07H19/04
CPC分类号： C07H21/00 , C07H19/04 , C07H21/04 , C12Q1/686
摘要： Propargylethoxyamino nucleosides are disclosed having structure (I), wherein R1 and R2 are -H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is -H or -OH; W2 is -OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position; and W3 is -PO4, -P2O7, -P3O10, phosphate analog, or -OH. Additionally, a primer extension method is provided employing the above propargylethoxyamino nucleosides.
摘要翻译：公开了具有结构（I）的丙炔乙氧基氨基核苷，其中R1和R2是-H，低级烷基或标记; B是7-脱氮嘌呤，嘌呤或嘧啶核苷碱基; W1是-H或-OH; W2是-OH或使核苷不能在3'-位形成磷酸二酯键的部分; W3是-PO 4，-P 2 O 7，-P 3 O 10，磷酸酯类似物或-OH。另外，使用上述炔丙氧基氨基核苷提供引物延伸方法。

2. 发明申请

WO1997036960A1 ASYMMETRIC BENZOXANTHENE DYES 审中-公开
标题翻译：不对称苯并噻嗪染料
公开(公告)号：WO1997036960A1
公开(公告)日：1997-10-09
申请号：PCT/US1997005376
申请日：1997-04-01
申请人： THE PERKIN-ELMER CORPORATION
发明人： THE PERKIN-ELMER CORPORATION , BENSON, Scott, C. , MENCHEN, Steven, M. , THEISEN, Peter, D. , HENNESSEY, Kevin, M. , FURNISS, Vergine, C. , HAUSER, Joan
IPC分类号： C09B11/24
CPC分类号： C07H21/00 , C07C39/38 , C09B11/00 , C09B11/08 , C12Q1/6869
摘要： A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having structure (I) wherein Y1 and Y2 are individually hydroxyl, amino, imminium, or oxygen, R1-R8 are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof, and R9 is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having general structure (II) where substituents R3-R7 correspond to like-referenced substituents in the structure of described above, and Y2 is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates. In yet another aspect, the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.
摘要翻译：公开了一类用作荧光染料的不对称单苯并氧蒽化合物，其结构（I）其中Y 1和Y 2分别是羟基，氨基，亚氨基或氧，R 1 -R 8是氢，氟，氯，烷基，烯烃，炔烃，氨基，酰氨基，腈，烷氧基，连接基团及其组合，R 9为乙炔，烷烃，烯烃，氰基，取代的苯基及其组合。本发明还包括可用于合成具有通式结构（II）的不对称苯并氧杂蒽化合物的新型中间体化合物，其中取代基R3-R7对应于上述结构中相似的取代基，而Y2是羟基或胺。另一方面，本发明包括合成上述染料化合物和中间体的方法。在另一方面，本发明包括用不对称苯并氧杂蒽染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸，亚磷酰胺和多核苷酸。在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

3. 发明授权

EP0658167B1 4,7-DICHLOROFLUORESCEIN DYES AS MOLECULAR PROBES 失效
标题翻译： 4,7- DICHLOROFLUOROSZEIN色材料的分子探针
公开(公告)号：EP0658167B1
公开(公告)日：1999-04-14
申请号：EP93920447.5
申请日：1993-09-01
申请人： THE PERKIN-ELMER CORPORATION
发明人： MENCHEN, Steven, M. , LEE, Linda, G. , CONNELL, Charles, R. , HERSHEY, N., Davis , CHAKERIAN, Vergine , WOO, Sam, L. , FUNG, Steven
IPC分类号： C07H21/00 , C07D311/82 , C07D493/10 , C12Q1/68
CPC分类号： C07H21/00 , C07D311/82 , C09B11/08 , C12Q1/6869 , C12Q2563/107
摘要： Long wavelength, narrow emission bandwidth fluorescein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5',7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.

4. 发明公开

EP0923597A1 SUBSTITUTED PROPARGYLETHOXYAMIDO NUCLEOSIDES 失效
标题翻译：取代的丙酰基乙酰氨基核苷
公开(公告)号：EP0923597A1
公开(公告)日：1999-06-23
申请号：EP98914275.0
申请日：1998-03-23
申请人： THE PERKIN-ELMER CORPORATION
发明人： KHAN, Shaheer, H. , MENCHEN, Steven, M. , ROSENBLUM, Barnett, B.
IPC分类号： C12N15 , C07H19 , C07H21 , C12Q1
CPC分类号： C07H21/00 , C07H19/04 , C12Q1/6869 , Y02P20/55
摘要： Substituted propargylethoxyamido nucleosides are disclosed having structure (I) wherein X is selected from the group consisting of amino alkanoic acid, alkylamino benzoic acid, α-amino acid, and 4-amino-2-butynoic acid. R1 and R2 taken separately are selected from the group consisting of -H, lower alkyl, protecting group, and label; R3 is selected from the group consisting of -H and lower alkyl. B is a 7-deazapurine, purine, or pyrimidine nucleoside base. When B is purine or 7-deazapurine, the sugar moiety is attached at the N9-position of the purine or deazapurine, and when B is pyrimidine, the sugar moiety is attached at the N1-position of the pyrimidine. When B is a purine, the adjacent triple-bonded carbon is attached to the 8-position of the purine, when B is 7-deazapurine, the adjacent triple-bonded carbon is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, the adjacent triple-bonded carbon is attached to the 5-position of the pyrimidine. W¿1? is selected from the group consisting of -H and -OH. W2 is -OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position. W3 is selected from the group consisting of -PO4, -P2O7, -P3O10, phosphate analog, and -OH. Additionally, a primer extension method is provided employing the above X-substituted propargylethoxyamido nucleosides, and polynucleotides including the above X-substituted propargylethoxyamido nucleosides is provided.
摘要翻译：公开了具有结构（I）的取代的炔丙基乙氧基酰胺核苷，其中X选自氨基链烷酸，烷基氨基苯甲酸，α-氨基酸和4-氨基-2-丁炔酸。 R1和R2分别选自-H，低级烷基，保护基和标记; R3选自-H和低级烷基。 B是7-脱氮嘌呤，嘌呤或嘧啶核苷碱基。当B是嘌呤或7-脱氮嘌呤时，糖部分连接在嘌呤或脱氮嘌呤的N9-位置，并且当B是嘧啶时，糖部分连接在嘧啶的N1位。当B是嘌呤时，相邻的三键碳连接到嘌呤的8-位，当B是7-脱氮嘌呤时，相邻的三键碳连接到7-脱氮嘌呤的7-位，并且当B是嘧啶时，相邻的三键碳连接到嘧啶的5-位。 W¿1？选自-H和-OH。 W2是-OH或使得核苷不能在3'-位形成磷酸二酯键的部分。 W3选自-PO4，-P2O7，-P3O10，磷酸盐类似物和-OH。此外，提供了使用上述X取代的炔丙基乙氧基酰胺基核苷的引物延伸方法，并且提供了包含上述X取代的炔丙基乙氧基酰胺核苷的多核苷酸。

5. 发明授权

EP0496749B1 SPECTRALLY RESOLVABLE RHODAMINE DYES FOR NUCLEIC ACID SEQUENCE DETERMINATION 失效
标题翻译：光谱可分辨的若丹明染料用于核酸序列测定。
公开(公告)号：EP0496749B1
公开(公告)日：1995-12-06
申请号：EP90914874.4
申请日：1990-09-27
申请人： THE PERKIN-ELMER CORPORATION
发明人： BERGOT, John, B. , CHAKERIAN, Vergine , CONNELL, Charles, R. , EADIE, J., Scott , FUNG, Steven , HERSHEY, Davis, N. , LEE, Linda, G. , MENCHEN, Steven, M. , WOO, Sam, L.
IPC分类号： C12Q1/68 , C07H19/10 , C07H19/20 , C07H21/04
CPC分类号： C12Q1/6869 , C07H21/00 , C09B11/24 , C12Q2535/101 , C12Q2563/107
摘要： A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucleotides used in the sequencing method by way of an alkynylamino linker. Preferably, the labeled dideoxynucleotides are incorporated into the growing DNA chains by Taq DNA polymerase.

6. 发明公开

EP0915901A1 PROPARGYLETHOXYAMINO NUCLEOTIDES 失效转让
标题翻译：丙基氨基甲酰胺核糖核酸
公开(公告)号：EP0915901A1
公开(公告)日：1999-05-19
申请号：EP97937953.0
申请日：1997-08-13
申请人： THE PERKIN-ELMER CORPORATION
发明人： KHAN, Shaheer, H. , MENCHEN, Steven, M. , ROSENBLUM, Barnett, B.
IPC分类号： C12N15 , C07H19 , C07H21 , C12Q1
CPC分类号： C07H21/00 , C07H19/04 , C07H21/04 , C12Q1/686
摘要： Propargylethoxyamino nucleosides are disclosed having structure (I), wherein R1 and R2 are -H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is -H or -OH; W2 is -OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position; and W3 is -PO4, -P2O7, -P3O10, phosphate analog, or -OH. Additionally, a primer extension method is provided employing the above propargylethoxyamino nucleosides.

7. 发明授权

EP0500779B1 4,7-DICHLOROFLUORESCEIN DYES AS MOLECULAR PROBES 失效
标题翻译： 4,7-二氯荧光素染料作为分子探针
公开(公告)号：EP0500779B1
公开(公告)日：1999-04-21
申请号：EP91900545.4
申请日：1990-11-13
申请人： THE PERKIN-ELMER CORPORATION
发明人： MENCHEN, Steven, M. , LEE, Linda, G. , CONNELL, Charles, R. , HERSHEY, N., Davis , CHAKERIAN, Vergine , WOO, Sam, Lee , FUNG, Steven
IPC分类号： C12Q1/68 , C07D311/82 , C07D311/90 , C07H21/00
CPC分类号： C12Q1/6869 , C07D311/82 , C07H21/00 , C09B11/08 , C12Q1/6816 , Y10S435/968 , Y10S436/80 , C12Q2563/107 , C12Q2535/101
摘要： Long wavelength, narrow emission bandwidth fluorescein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 1',2',7',8'-dibenzo-4,7-dichlorofluoresceins. Methods of using the dyes in automated DNA sequencing are described.
摘要翻译：提供长波长，窄发射带宽的荧光素染料用于检测空间上重叠的目标物质。染料包含4,7-二氯荧光素，特别是1'，2'，7'，8'-二苯并-4,7-二氯荧光素。描述了在自动化DNA测序中使用染料的方法。

8. 发明公开

EP0891393A1 ASYMMETRIC BENZOXANTHENE DYES 有权转让
标题翻译：亚甲基苯并噻唑
公开(公告)号：EP0891393A1
公开(公告)日：1999-01-20
申请号：EP97920028.0
申请日：1997-04-01
申请人： THE PERKIN-ELMER CORPORATION
发明人： BENSON, Scott, C. , MENCHEN, Steven, M. , THEISEN, Peter, D. , HENNESSEY, Kevin, M. , FURNISS, Vergine, C. , HAUSER, Joan
IPC分类号： C12N15 , C07C39 , C07D311 , C07F9 , C07H19 , C07H21 , C09B11 , C09K11 , C12Q1 , G01N33
CPC分类号： C07H21/00 , C07C39/38 , C09B11/00 , C09B11/08 , C12Q1/6869
摘要： A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having structure (I) wherein Y1 and Y2 are individually hydroxyl, amino, imminium, or oxygen, R1-R8 are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof, and R9 is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having general structure (II) where substituents R3-R7 correspond to like-referenced substituents in the structure of described above, and Y2 is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates. In yet another aspect, the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

9. 发明授权

EP0915901B1 PROPARGYLETHOXYAMINO NUCLEOTIDES 失效转让
标题翻译：丙酰氨基核糖核苷酸
公开(公告)号：EP0915901B1
公开(公告)日：2000-10-11
申请号：EP97937953.4
申请日：1997-08-13
申请人： THE PERKIN-ELMER CORPORATION
发明人： KHAN, Shaheer, H. , MENCHEN, Steven, M. , ROSENBLUM, Barnett, B.
IPC分类号： C07H19/04 , C07H21/00 , C12Q1/68
CPC分类号： C07H21/00 , C07H19/04 , C07H21/04 , C12Q1/686

10. 发明授权

EP0891393B1 ASYMMETRIC BENZOXANTHENE DYES 有权转让
标题翻译：不对称苯并呫
公开(公告)号：EP0891393B1
公开(公告)日：1999-06-30
申请号：EP97920028.4
申请日：1997-04-01
申请人： THE PERKIN-ELMER CORPORATION
发明人： BENSON, Scott, C. , MENCHEN, Steven, M. , THEISEN, Peter, D. , HENNESSEY, Kevin, M. , FURNISS, Vergine, C. , HAUSER, Joan
IPC分类号： C09B11/24 , C09B11/28 , C07H21/00 , C12Q1/68 , G01N33/52 , C09B11/08 , C07C39/14 , C07C39/38
CPC分类号： C07H21/00 , C07C39/38 , C09B11/00 , C09B11/08 , C12Q1/6869
摘要： A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having structure (I) wherein Y1 and Y2 are individually hydroxyl, amino, imminium, or oxygen, R1-R8 are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof, and R9 is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having general structure (II) where substituents R3-R7 correspond to like-referenced substituents in the structure of described above, and Y2 is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates. In yet another aspect, the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

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