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1. 发明申请

WO2018231890A1 CORN ELITE EVENT MZIR098 审中-公开
公开(公告)号：WO2018231890A1
公开(公告)日：2018-12-20
申请号：PCT/US2018/037189
申请日：2018-06-13
申请人： SYNGENTA PARTICIPATIONS AG , SYNGENTA CROP PROTECTION, LLC
发明人： NOE, Andrew , CONVILLE, Jared , GU, Weining , JIANG, Yaping , CARLIN, Ryan William , YU, Wenjin , MITTENDORF, Volker
IPC分类号： C12N15/82 , C12Q1/6895
摘要： A novel transgenic corn elite event designated MZIR098 is disclosed. The invention relates to DNA sequences of the recombinant constructs inserted into the corn genome and of genomic sequences flanking the insertion site that resulted in elite event MZIR098. The invention further relates to assays for detecting the presence of the DNA sequences of corn elite event MZIR098, to corn plants and corn seeds comprising the genotype thereof, and to methods for producing a corn plant by crossing a corn plant comprising the elite event MZIR098 genotype with itself or another corn variety.

2. 发明申请

WO2016153645A1 A NUCLEIC ACID CONSTRUCT FOR CONFERRING HERBICIDE TOLERANCE IN PLANTS 审中-公开
标题翻译：用于在植物中认定除草剂耐受性的核酸结构
公开(公告)号：WO2016153645A1
公开(公告)日：2016-09-29
申请号：PCT/US2016/018432
申请日：2016-02-18
申请人： SYNGENTA PARTICIPATIONS AG , SYNGENTA CROP PROTECTION, LLC
发明人： MITTENDORF, Volker , HIPSKIND, John , AZHAKANANDAM, Kasi
IPC分类号： C07K14/415
CPC分类号： C12N15/8277 , C12N9/1029 , C12N9/1085 , C12N9/1092 , C12N15/8275 , C12Y203/01183 , C12Y205/01019
摘要： The present invention is drawn to a nucleic acid sequence which confers expression of a phosphinothricin acetyltransferase and a 5-enolpyruvyl-3-phosphoshikimic acid synthase when introduced into a cell. These proteins may confer herbicide tolerance.
摘要翻译：本发明涉及一种当引入细胞时赋予膦丝菌蛋白乙酰转移酶和5-烯醇丙酮酰-3-磷酸嘧啶酸合酶表达的核酸序列。这些蛋白质可以赋予除草剂耐受性。

3. 发明申请

WO2017214074A1 CORN ELITE EVENT MZHG0JG 审中-公开
标题翻译：玉米精华活动MZHG0JG
公开(公告)号：WO2017214074A1
公开(公告)日：2017-12-14
申请号：PCT/US2017/036035
申请日：2017-06-06
申请人： SYNGENTA PARTICIPATIONS AG
发明人： MITTENDORF, Volker , GU, Weining , BEANBLOSSOM, Shawn , NOE, Andrew , CARLIN, Ryan William , JIANG, Yaping , YU, Wenjin
IPC分类号： A01H5/00 , C12N15/09 , C12N15/29 , C12N15/82 , C12Q1/68
CPC分类号： C12N15/8277 , C12N15/8275 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： A novel transgenic corn elite event designated MZHG0JG is disclosed. The invention relates to nucleic acid sequences of the recombinant constructs inserted into the corn genome and to the genomic sequences flanking the insertion site, which resulted in the elite event MZHG0JG that comprises nucleic acid molecules containing novel nucleic acid sequences. The invention further relates to assays for detecting the presence of the novel nucleic acid sequences of elite event MZHG0JG, to corn plants and corn seeds comprising the genotype thereof, and to methods for producing a corn plant by crossing a corn plant comprising the elite event MZHG0JG genotype with itself or another corn variety.
摘要翻译：公开了称为MZHG0JG的新型转基因玉米精英事件。本发明涉及插入玉米基因组中的重组构建体的核酸序列以及插入位点侧翼的基因组序列，其导致包含含有新核酸序列的核酸分子的精英事件MZHG0JG。本发明还涉及到了检测原种事件MZHG0JG的新的核酸序列的存在，对玉米植物和玉米种子包含其基因型，以及用于通过杂交包含原种事件MZHG0JG的玉米植物产生的玉米植物的方法基因型与自身或其他玉米品种。

4. 发明申请

WO2016209360A1 A NUCLEIC ACID MOLECULE FOR CONFERRING INSECTICIDAL PROPERTIES IN PLANTS 审中-公开
标题翻译：用于在植物中认定杀虫剂性质的核酸分子
公开(公告)号：WO2016209360A1
公开(公告)日：2016-12-29
申请号：PCT/US2016/029424
申请日：2016-04-27
申请人： SYNGENTA PARTICIPATIONS AG , SYNGENTA CROP PROTECTION LLC
发明人： MITTENDORF, Volker , CONVILLE, Jared , HIPSKIND, John Daniel , AZHAKANANDAM, Kasimalai , NOE, Andrew , FEI, Xiaoyin , DONOHUE, Kevin V.
IPC分类号： C07K14/325 , C12N15/32 , C12N15/82 , C12N5/04 , A01H5/00
CPC分类号： C07K14/325 , A01H1/02 , C12N15/8286 , Y02A40/162
摘要： The present invention is drawn to a nucleic acid sequence which confers expression of the insecticidal proteins mCry3A and eCry3.1Ab when introduced into a cell.
摘要翻译：本发明涉及一种当引入细胞时赋予杀虫蛋白mCry3A和eCry3.1Ab表达的核酸序列。

5. 发明申请

WO2015119719A1 GENOMIC DNA EXTRACTION REAGENT AND METHOD 审中-公开
标题翻译：基因DNA提取试剂和方法
公开(公告)号：WO2015119719A1
公开(公告)日：2015-08-13
申请号：PCT/US2014/072151
申请日：2014-12-23
申请人： SYNGENTA PARTICIPATIONS AG , JI, Yanshan , FEI, Xiaoyin , YU, Wenjin , MITTENDORF, Volker
发明人： JI, Yanshan , FEI, Xiaoyin , YU, Wenjin , MITTENDORF, Volker
IPC分类号： C12N15/10
CPC分类号： C12N15/1003
摘要： The present invention is directed to a genomic DNA extraction reagent and method for improved extraction of DNA from biological tissue. The extraction reagent of the invention is mixed with disrupted biological tissue to form a DNA extraction solute which is incubated in a DNA extraction step. The extraction reagent includes an alkali component to maintain the DNA extraction solute at a pH of about 10 to 14 substantially throughout the extraction step. The extraction solute is centrifuged to clarify the supernatant. The supernatant containing the extracted DNA is diluted with a neutralizing buffer resulting in a high throughout method of generating high quantities of high quality DNA. Major PCR inhibitors are managed with the unique chemical combinations of the DNA extraction reagent designed and optimized for extraction of DNA from plant tissue and cells.
摘要翻译：本发明涉及用于从生物组织中提取DNA的基因组DNA提取试剂和方法。将本发明的提取试剂与破碎的生物组织混合以形成在DNA提取步骤中温育的DNA提取溶质。提取试剂包括碱性组分，以在整个提取步骤中基本上保持DNA提取溶质在约10至14的pH。将提取溶质离心以澄清上清液。将含有提取的DNA的上清液用中和缓冲液稀释，导致产生大量高质量DNA的全部方法。主要的PCR抑制剂通过设计和优化用于从植物组织和细胞中提取DNA的DNA提取试剂的独特化学组合进行管理。

6. 发明公开

EP3638795A1 CORN ELITE EVENT MZIR098 审中-实审转让
公开(公告)号：EP3638795A1
公开(公告)日：2020-04-22
申请号：EP18816765.4
申请日：2018-06-13
申请人： Syngenta Participations AG
发明人： NOE, Andrew , CONVILLE, Jared , GU, Weining , JIANG, Yaping , CARLIN, Ryan William , YU, Wenjin , MITTENDORF, Volker
IPC分类号： C12N15/82 , C12Q1/6895

7. 发明公开

EP3277709A1 A NUCLEIC ACID CONSTRUCT FOR CONFERRING HERBICIDE TOLERANCE IN PLANTS 审中-公开
标题翻译：一种用于确定植物中除草剂耐受性的核酸构建体
公开(公告)号：EP3277709A1
公开(公告)日：2018-02-07
申请号：EP16769231.8
申请日：2016-02-18
申请人： Syngenta Participations AG
发明人： MITTENDORF, Volker , HIPSKIND, John , AZHAKANANDAM, Kasi
IPC分类号： C07K14/415
CPC分类号： C12N15/8277 , C12N9/1029 , C12N9/1085 , C12N9/1092 , C12N15/8275 , C12Y203/01183 , C12Y205/01019
摘要： The present invention is drawn to a nucleic acid sequence which confers expression of a phosphinothricin acetyltransferase and a 5-enolpyruvyl-3-phosphoshikimic acid synthase when introduced into a cell. These proteins may confer herbicide tolerance.

8. 发明公开

EP3102677A1 GENOMIC DNA EXTRACTION REAGENT AND METHOD 审中-公开
标题翻译：一般来说，DNA-EXTRAKTIONSREAGENZ在全球范围内
公开(公告)号：EP3102677A1
公开(公告)日：2016-12-14
申请号：EP14881909.7
申请日：2014-12-23
申请人： Syngenta Participations AG
发明人： JI, Yanshan , FEI, Xiaoyin , YU, Wenjin , MITTENDORF, Volker
IPC分类号： C12N15/10
CPC分类号： C12N15/1003
摘要： The present invention is directed to a genomic DNA extraction reagent and method for improved extraction of DNA from biological tissue. The extraction reagent of the invention is mixed with disrupted biological tissue to form a DNA extraction solute which is incubated in a DNA extraction step. The extraction reagent includes an alkali component to maintain the DNA extraction solute at a pH of about 10 to 14 substantially throughout the extraction step. The extraction solute is centrifuged to clarify the supernatant. The supernatant containing the extracted DNA is diluted with a neutralizing buffer resulting in a high throughout method of generating high quantities of high quality DNA. Major PCR inhibitors are managed with the unique chemical combinations of the DNA extraction reagent designed and optimized for extraction of DNA from plant tissue and cells.
摘要翻译：本发明涉及用于从生物组织中提取DNA的基因组DNA提取试剂和方法。将本发明的提取试剂与破碎的生物组织混合以形成在DNA提取步骤中温育的DNA提取溶质。提取试剂包括碱性组分，以在整个提取步骤中基本上保持DNA提取溶质在约10至14的pH。将提取溶质离心以澄清上清液。将含有提取的DNA的上清液用中和缓冲液稀释，导致产生大量高质量DNA的全部方法。主要的PCR抑制剂通过设计和优化用于从植物组织和细胞中提取DNA的DNA提取试剂的独特化学组合进行管理。

9. 发明公开

EP3313867A1 A NUCLEIC ACID MOLECULE FOR CONFERRING INSECTICIDAL PROPERTIES IN PLANTS 有权转让
标题翻译：一种用于防治植物杀虫性质的核酸分子
公开(公告)号：EP3313867A1
公开(公告)日：2018-05-02
申请号：EP16814863.3
申请日：2016-04-27
申请人： Syngenta Participations AG
发明人： MITTENDORF, Volker , CONVILLE, Jared , HIPSKIND, John Daniel , AZHAKANANDAM, Kasimalai , NOE, Andrew , FEI, Xiaoyin , DONOHUE, Kevin V.
IPC分类号： C07K14/325 , C12N15/32 , C12N15/82 , C12N5/04 , A01H5/00
CPC分类号： C07K14/325 , A01H1/02 , C12N15/8286 , Y02A40/162
摘要： The present invention is drawn to a nucleic acid sequence which confers expression of the insecticidal proteins mCry3A and eCry3.1Ab when introduced into a cell.

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