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1. 发明专利

CA2901406A1 GENOMIC DNA EXTRACTION REAGENT AND METHOD 未知
公开(公告)号：CA2901406A1
公开(公告)日：2015-08-13
申请号：CA2901406
申请日：2014-12-23
申请人： SYNGENTA PARTICIPATIONS AG , JI YANSHAN , FEI XIAOYIN , YU WENJIN , MITTENDORF VOLKER
发明人： JI YANSHAN , FEI XIAOYIN , YU WENJIN , MITTENDORF VOLKER
IPC分类号： C12N15/10
摘要： The present invention is directed to a genomic DNA extraction reagent and method for improved extraction of DNA from biological tissue. The extraction reagent of the invention is mixed with disrupted biological tissue to form a DNA extraction solute which is incubated in a DNA extraction step. The extraction reagent includes an alkali component to maintain the DNA extraction solute at a pH of about 10 to 14 substantially throughout the extraction step. The extraction solute is centrifuged to clarify the supernatant. The supernatant containing the extracted DNA is diluted with a neutralizing buffer resulting in a high throughout method of generating high quantities of high quality DNA. Major PCR inhibitors are managed with the unique chemical combinations of the DNA extraction reagent designed and optimized for extraction of DNA from plant tissue and cells.

2. 发明申请

WO2015119719A1 GENOMIC DNA EXTRACTION REAGENT AND METHOD 审中-公开
标题翻译：基因DNA提取试剂和方法
公开(公告)号：WO2015119719A1
公开(公告)日：2015-08-13
申请号：PCT/US2014/072151
申请日：2014-12-23
申请人： SYNGENTA PARTICIPATIONS AG , JI, Yanshan , FEI, Xiaoyin , YU, Wenjin , MITTENDORF, Volker
发明人： JI, Yanshan , FEI, Xiaoyin , YU, Wenjin , MITTENDORF, Volker
IPC分类号： C12N15/10
CPC分类号： C12N15/1003
摘要： The present invention is directed to a genomic DNA extraction reagent and method for improved extraction of DNA from biological tissue. The extraction reagent of the invention is mixed with disrupted biological tissue to form a DNA extraction solute which is incubated in a DNA extraction step. The extraction reagent includes an alkali component to maintain the DNA extraction solute at a pH of about 10 to 14 substantially throughout the extraction step. The extraction solute is centrifuged to clarify the supernatant. The supernatant containing the extracted DNA is diluted with a neutralizing buffer resulting in a high throughout method of generating high quantities of high quality DNA. Major PCR inhibitors are managed with the unique chemical combinations of the DNA extraction reagent designed and optimized for extraction of DNA from plant tissue and cells.
摘要翻译：本发明涉及用于从生物组织中提取DNA的基因组DNA提取试剂和方法。将本发明的提取试剂与破碎的生物组织混合以形成在DNA提取步骤中温育的DNA提取溶质。提取试剂包括碱性组分，以在整个提取步骤中基本上保持DNA提取溶质在约10至14的pH。将提取溶质离心以澄清上清液。将含有提取的DNA的上清液用中和缓冲液稀释，导致产生大量高质量DNA的全部方法。主要的PCR抑制剂通过设计和优化用于从植物组织和细胞中提取DNA的DNA提取试剂的独特化学组合进行管理。

3. 发明申请

WO2014133732A1 METHODS AND COMPOSITIONS FOR PREPARATION OF NUCLEIC ACIDS 审中-公开
标题翻译：制备核酸的方法和组合物
公开(公告)号：WO2014133732A1
公开(公告)日：2014-09-04
申请号：PCT/US2014/015474
申请日：2014-02-10
申请人： SYNGENTA PARTICIPATIONS AG , JI, Yanshan , WANG, Wenling , HUANG, Er-Te Jamie , MOORE, Karen Elizabeth , WHITE, Kimberly Ann
发明人： JI, Yanshan , WANG, Wenling , HUANG, Er-Te Jamie , MOORE, Karen Elizabeth , WHITE, Kimberly Ann
IPC分类号： A01H5/00 , C12Q1/68 , C12N1/06 , C12N15/10 , C12P19/34
CPC分类号： C12N15/1003
摘要： A method for isolating genomic DNA (gDNA) from a biological material, the method including (a) contacting a sample that contains gDNA with a solution of hydroxide and a detergent to denature the gDNA; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and reducing the pH of the solution to less than 1 0, thereby producing a neutralized solution; (c) centrifuging the sample to clarify the preparation; and (d) removing Insoluble material from the neutralized and clarified preparation, whereby a solution of gDNA is produced.
摘要翻译：一种从生物材料中分离基因组DNA（gDNA）的方法，所述方法包括（a）使含有gDNA的样品与氢氧化物和洗涤剂的溶液接触以使所述gDNA变性; （b）将由步骤（a）得到的溶液混合成以高盐为特征的溶液，并将溶液的pH降低到小于10，由此产生中和的溶液; （c）离心样品澄清制剂; 和（d）从中和澄清的制剂中除去不溶物，由此产生gDNA溶液。

4. 发明申请

WO2013012775A1 CORN EVENT MZDT09Y 审中-公开
标题翻译： CORN活动MZDT09Y
公开(公告)号：WO2013012775A1
公开(公告)日：2013-01-24
申请号：PCT/US2012/046852
申请日：2012-07-16
申请人： SYNGENTA PARTICIPATIONS AG , CADE, Rebecca , DUNCAN, Kateri , NUCCIO, Michael , GU, Weining , JI, Yanshan , FAN, Chunyang , DIMAIO, Joseph , ALTENDORF, Paul , YU, Wenjin
发明人： CADE, Rebecca , DUNCAN, Kateri , NUCCIO, Michael , GU, Weining , JI, Yanshan , FAN, Chunyang , DIMAIO, Joseph , ALTENDORF, Paul , YU, Wenjin
IPC分类号： C12N15/82 , C12N9/16 , C12Q1/68
CPC分类号： C12N15/8273 , C12N9/16 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Y204/01216
摘要： A transgenic corn event designated MZDT09Y is disclosed. The invention relates to nucleic acids that are unique to event MZDT09Y and to methods of detecting the presence of event MZDT09Y based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MZDT09Y event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of event MZDT09Y and to methods for producing a corn plant by cross¬ ing a corn plant comprising the MZDT09Y genotype with itself or another corn variety. Seeds of corn plants comprising the MZDT09Y genotype are also objects of the invention.
摘要翻译：公开了一种称为MZDT09Y的转基因玉米事件。本发明涉及事件MZDT09Y特有的核酸以及基于插入到导致MZDT09Y事件的玉米基因组中的重组构建体的DNA序列和位于插入位点侧翼的基因组序列的事件MZDT09Y的存在的方法。本发明还涉及包含事件MZDT09Y的转基因基因型的玉米植物和通过将包含MZDT09Y基因型的玉米植物与其本身或另一种玉米品种交叉的玉米植物的生产方法。包含MZDT09Y基因型的玉米植物种子也是本发明的目的。

5. 发明申请

WO0204613A3 ISOLATED POLYNUCLEOTIDES ENCODING HUMAN 5'-NUCLEOTIDE CN-IA AND CN-IB, ISOLATED PROTEINS ENCODED BY THE SAME, AND METHODS UTILIZING THE SAME 审中-公开
标题翻译：编码人5'-核酸CN-IA和CN-IB的分离多核苷酸，由其编码的分离蛋白，以及使用其的方法
公开(公告)号：WO0204613A3
公开(公告)日：2003-02-13
申请号：PCT/US0121406
申请日：2001-07-06
申请人： UNIV NORTH CAROLINA , SPYCHALA JOZEF , MITCHELL BEVERLY S , HUNSUCKER SALLY , JI YANSHAN
发明人： SPYCHALA JOZEF , MITCHELL BEVERLY S , HUNSUCKER SALLY , JI YANSHAN
IPC分类号： A61K38/00 , C12N1/21 , C12N9/16 , C12N15/55 , A61K31/7072 , C07K16/40 , G01N33/573
CPC分类号： C12N9/16 , A61K38/00
摘要： The isolated polynucleotide sequence identified herein as SEQ ID NO: 1 encodes an human cytosolic 5'-nucleotidase form I (human cN-IA) and encodes the protein human cN-IA of SEQ ID NO:2. The isolated polynucleotide sequence identified herein as SEQ ID NO: 3 encodes a cytosolic 5'-nucleotidase form I (cN-IB) and encodes the protein cN-IB of SEQ ID NO:4. Expression of human cN-IA decreases the efficacy of antineoplastic drugs and increases the toxicity of AZT. Inhibitors of human cN-IA are useful in methods of treating cancer by increasing the efficacy of antineoplastic drugs, and in methods of treating viral infections such as HIV infection and AIDS by decreasing the side effects of AZT.
摘要翻译：本文鉴定为SEQ ID NO：1的分离的多核苷酸序列编码人类胞质5'-核苷酸酶I（人cN-IA），并编码SEQ ID NO：2的蛋白质人cN-IA。本文鉴定为SEQ ID NO：3的分离的多核苷酸序列编码细胞溶质5'-核苷酸酶I（cN-IB），并编码SEQ ID NO：4的蛋白cN-IB。人cN-IA的表达降低抗肿瘤药物的功效，增加AZT的毒性。人cN-IA的抑制剂可用于通过增加抗肿瘤药物的疗效来治疗癌症的方法，以及通过降低AZT的副作用来治疗HIV感染和AIDS的病毒感染的方法。

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