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1. 发明授权

US06524856B1 Use of consensus sequences for targeted homologous gene isolation and recombination in gene families 失效
标题翻译：在基因家族中使用共有序列进行靶向同源基因分离和重组
公开(公告)号：US06524856B1
公开(公告)日：2003-02-25
申请号：US09209676
申请日：1998-12-11
申请人： Sushma Pati , David A. Zarling , Hong Zeng
发明人： Sushma Pati , David A. Zarling , Hong Zeng
IPC分类号： C12N1563
CPC分类号： C12N15/1082 , A01K2217/05 , A61K48/00 , C12N15/1034 , C12N15/902 , C12Q1/6811
摘要： The invention relates to compositions and methods for targeting sequence modifications in one or more genes of a related family of genes using enhanced homologous recombination techniques. These techniques may be used to create animal or plant models of disease as well as to identify new targets for drug or pathogen screening.
摘要翻译：本发明涉及使用增强的同源重组技术靶向相关基因家族的一个或多个基因中的序列修饰的组合物和方法。这些技术可用于创建疾病的动物或植物模型，以及确定用于药物或病原体筛选的新靶标。

2. 发明授权

US6074853A Sequence alterations using homologous recombination 失效
标题翻译：使用同源重组的序列改变
公开(公告)号：US6074853A
公开(公告)日：2000-06-13
申请号：US133934
申请日：1998-08-14
申请人： Sushma Pati , David A. Zarling
发明人： Sushma Pati , David A. Zarling
IPC分类号： C12N15/09 , A61K48/00 , C12N15/10 , C12N15/90 , C12Q1/68 , C12P19/34 , C12N15/00
CPC分类号： C12N15/907 , A61K48/00 , C12N15/102 , C12N15/1027 , C12N15/90
摘要： The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell. The invention also relates to compositions that contain exogenous targeting polynucleotides, complementary pairs of exogenous targeting polynucleotides, chemical substituents of such polynucleotides, and recombinase proteins used in the methods of the invention.
摘要翻译：本发明涉及通过同源配对将外源多核苷酸或外源互补多核苷酸对靶向靶向细胞中预定的内源性DNA靶序列的方法，特别是用于通常通过靶向同源重组来改变内源DNA序列，例如染色体DNA序列。在某些实施方案中，本发明涉及将具有连接的化学取代基的外源多核苷酸靶向代谢活性靶细胞中的预定内源性DNA序列的方法，产生在完整核中的一个或多个化学取代基的DNA序列特异性靶向通常用于改变细胞中预定的内源性DNA序列的代谢活性靶细胞。本发明还涉及含有外源靶向多核苷酸，外源靶向多核苷酸的互补对，这种多核苷酸的化学取代基和本发明方法中使用的重组酶的组合物。

3. 发明授权

US5948653A Sequence alterations using homologous recombination 失效
公开(公告)号：US5948653A
公开(公告)日：1999-09-07
申请号：US910367
申请日：1997-08-13
申请人： Sushma Pati , David A. Zarling
发明人： Sushma Pati , David A. Zarling
IPC分类号： C12N15/09 , A61K48/00 , C12N15/10 , C12N15/90 , C12Q1/68 , C07H21/04 , C07K14/00 , C12N15/00 , C12P19/34
CPC分类号： C12N15/907 , A61K48/00 , C12N15/102 , C12N15/1027 , C12N15/90
摘要： The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell. The invention also relates to compositions that contain exogenous targeting polynucleotides, complementary pairs of exogenous targeting polynucleotides, chemical substituents of such polynucleotides, and recombinase proteins used in the methods of the invention.

4. 发明授权

US06200812B1 Sequence alterations using homologous recombination 有权
标题翻译：使用同源重组的序列改变
公开(公告)号：US06200812B1
公开(公告)日：2001-03-13
申请号：US09288586
申请日：1999-04-08
申请人： Sushma Pati , David A. Zarling
发明人： Sushma Pati , David A. Zarling
IPC分类号： C12N1563
CPC分类号： C12N15/907 , A61K48/00 , C12N15/102 , C12N15/1027 , C12N15/90
摘要： The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell. The invention also relates to compositions that contain exogenous targeting polynucleotides, complementary pairs of exogenous targeting polynucleotides, chemical substituents of such polynucleotides, and recombinase proteins used in the methods of the invention.
摘要翻译：本发明涉及通过同源配对将外源多核苷酸或外源互补多核苷酸对靶向靶向细胞中预定的内源性DNA靶序列的方法，特别是用于通常通过靶向同源重组来改变内源DNA序列，例如染色体DNA序列。在某些实施方案中，本发明涉及将具有连接的化学取代基的外源多核苷酸靶向代谢活性靶细胞中的预定内源性DNA序列的方法，产生在完整核中的一个或多个化学取代基的DNA序列特异性靶向通常用于改变细胞中预定的内源性DNA序列的代谢活性靶细胞。本发明还涉及含有外源靶向多核苷酸，外源靶向多核苷酸的互补对，这种多核苷酸的化学取代基和本发明方法中使用的重组酶的组合物。

5. 发明申请

US20060228799A1 Use of consensus sequences for targeted homologous gene isolation and recombination in gene families 审中-公开
公开(公告)号：US20060228799A1
公开(公告)日：2006-10-12
申请号：US11244325
申请日：2005-10-05
申请人： Sushma Pati , David Zarling , Christopher Lehman , Hong Zeng
发明人： Sushma Pati , David Zarling , Christopher Lehman , Hong Zeng
IPC分类号： C12N15/86 , C12N15/74
CPC分类号： C12N15/1093 , C12N15/902
摘要： The invention relates to compositions and methods for targeting sequence modifications in one or more genes of a related family of genes using enhanced homologous recombination techniques. These techniques may be used to create animal or plant models of disease as well as to identify new targets for drug or pathogen screening.

6. 发明申请

US20050214944A1 In vivo homologous sequence targeting in cells 审中-公开
标题翻译：体内同源序列靶向细胞
公开(公告)号：US20050214944A1
公开(公告)日：2005-09-29
申请号：US10973209
申请日：2004-10-25
申请人： David Zarling , Elissa Sena , Sushma Pati
发明人： David Zarling , Elissa Sena , Sushma Pati
IPC分类号： A01K67/027 , A61K48/00 , C07K14/47 , C12N9/10 , C12N15/10 , C12N15/74 , C12N15/82 , C12N15/85 , C12N15/90
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2217/075 , A61K48/00 , A61K48/005 , C07K14/4712 , C07K14/4746 , C12N9/1018 , C12N15/102 , C12N15/8213 , C12N15/90 , C12N15/902 , C12N15/907
摘要： The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell. The invention also relates to compositions that contain exogenous targeting polynucleotides, complementary pairs of exogenous targeting polynucleotides, chemical substituents of such polynucleotides, and recombinase proteins used in the methods of the invention.
摘要翻译：本发明涉及通过同源配对将外源多核苷酸或外源互补多核苷酸对靶向靶向细胞中预定的内源性DNA靶序列的方法，特别是用于通常通过靶向同源重组来改变内源DNA序列，例如染色体DNA序列。在某些实施方案中，本发明涉及将具有连接的化学取代基的外源多核苷酸靶向代谢活性靶细胞中的预定内源性DNA序列的方法，产生在完整核中的一个或多个化学取代基的DNA序列特异性靶向通常用于改变细胞中预定的内源性DNA序列的代谢活性靶细胞。本发明还涉及含有外源靶向多核苷酸，外源靶向多核苷酸的互补对，这种多核苷酸的化学取代基和本发明方法中使用的重组酶的组合物。

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