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1. 发明专利

JP2014168387A Reductase gene amplification primer set, and reductase gene acquisition method 有权
标题翻译：降低基因扩增引物集和降低基因获得方法
公开(公告)号：JP2014168387A
公开(公告)日：2014-09-18
申请号：JP2013040378
申请日：2013-03-01
申请人： Sumitomo Chemical Co Ltd , 住友化学株式会社 , Toyama Prefecture , 富山県
发明人： ITO SHINYA , KUROKAWA JUNJI , ASAKO HIROYUKI
IPC分类号： C12N15/09 , C12N1/21 , C12N9/02
摘要： PROBLEM TO BE SOLVED: To provide a method for efficiently acquiring a nucleic acid which encodes a reductase, and also to provide a nucleic acid encoding reductase and the like acquirable by the method.SOLUTION: A method and the like for acquiring a nucleic acid encoding a reductase are provided, the method comprising: (1) performing a nucleic acid amplification reaction with a DNA sample as a template to acquire an amplification product; (2) fusing the amplification product and a double-stranded vector DNA functionable as an expression vector in a host cell upon cyclization in the presence of a DNA polymerase having an endogenous 3'-5' exonuclease activity to acquire a cyclic recombinant expression vector containing the amplification product; (3) introducing the cyclic recombinant expression vector into the host cell to acquire transformants; (4) selecting a transformant having an activity for reducing the substrate; and (5) acquiring a nucleic acid which encodes a reductase having the activity, from the transformant selected in the step (4).
摘要翻译：要解决的问题：提供一种有效获得编码还原酶的核酸的方法，并且还提供通过该方法获得的编码还原酶等的核酸。解决方案：用于获得核酸编码的方法等提供还原酶，该方法包括：（1）以DNA样品作为模板进行核酸扩增反应以获得扩增产物; （2）在具有内源3'-5'核酸外切酶活性的DNA聚合酶存在下，将扩增产物和可作为表达载体作为表达载体的双链载体DNA在宿主细胞中进行环化以获得含有扩增产物; （3）将环状重组表达载体导入宿主细胞以获得转化体; （4）选择具有降低底物活性的转化体; 和（5）从步骤（4）中选择的转化体获得编码具有活性的还原酶的核酸。

2. 发明专利

JP2013172682A Mutation-type reductase and utilization thereof 审中-公开
标题翻译：突变型还原及其利用
公开(公告)号：JP2013172682A
公开(公告)日：2013-09-05
申请号：JP2012039796
申请日：2012-02-27
申请人： Toyama Prefecture , 富山県 , Sumitomo Chemical Co Ltd , 住友化学株式会社
发明人： ITO SHINYA , ASAKO HIROYUKI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12P7/06
CPC分类号： Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide a mutation-type reductase utilizable in a reduction reaction, and to provide the utilization or the like of the reductase.SOLUTION: There is provided an enzyme having an amino acid sequence equivalent to an original amino acid sequence except having four or more amino acid mutations including the whole of the following basic amino acid mutations (a) to (d) in a specific amino acid sequence originated from Rhodococcus erythropolis, and further having the ability for reducing a substrate. : (a) the amino acid mutation by the substitution of the 12th glutamic acid with glycine; (b) the amino acid mutation by the substitution of the 42th aspartic acid with valine; (c) the amino acid mutation by the substitution of the 67th lysine with arginine; and (d) the amino acid mutation by the substitution of the 317th serine with alanine.
摘要翻译：要解决的问题：提供可用于还原反应的突变型还原酶，并提供还原酶的利用等。溶液：提供具有与原始氨基酸序列等同的氨基酸序列的酶，除了具有四个或更多个氨基酸突变，包括源自红景天红细菌的特定氨基酸序列中的全部以下碱性氨基酸突变（a）至（d），并且还具有还原底物的能力。 <碱性氨基酸突变>：（a）用甘氨酸取代第十二谷氨酸的氨基酸突变; （b）用缬氨酸取代第42天冬氨酸的氨基酸突变; （c）用精氨酸代替第67位赖氨酸的氨基酸突变; 和（d）通过用丙氨酸取代第317位丝氨酸的氨基酸突变。

3. 发明专利

JP2012010635A METHOD FOR PRODUCING SULFUR-CONTAINING α-AMINO ACID COMPOUND 审中-公开
标题翻译：生产含硫酸的α-氨基酸化合物的方法
公开(公告)号：JP2012010635A
公开(公告)日：2012-01-19
申请号：JP2010149338
申请日：2010-06-30
申请人： Sumitomo Chemical Co Ltd , 住友化学株式会社
发明人： ASAKO HIROYUKI
IPC分类号： C12P13/04 , C12P13/12 , C12R1/645
CPC分类号： C12P13/04 , C12P13/12
摘要： PROBLEM TO BE SOLVED: To provide a new method for producing a sulfur-containing α-amino acid compound such as methionine, etc.SOLUTION: The method for producing a sulfur-containing α-amino acid compound represented by the general formula: R-S-CH-CH-CH(NH)-COOH includes a first step of previously culturing a microorganism capable of converting a sulfur-containing amino alcohol compound represented by general formula (1) (wherein Ris hydrogen, 1-8C alkyl or 6-20C aryl) into the corresponding sulfur-containing α-amino acid compound in a culture medium containing a lower aliphatic alcohol to prepare a microbial cell of the microorganism; and a second step of reacting the sulfur-containing amino alcohol compound with the microbial cell of the microorganism obtained in the first step or a processed product of the microbial cell.
摘要翻译：要解决的问题：提供一种生产含硫α-氨基酸化合物如甲硫氨酸等的新方法。解决方案：用于制备由下式表示的含硫α-氨基酸化合物的方法：通式：R 1 -S-CH 2 -CH 2 -CH（NH）（CO 2）COOH包括第一步，其先前培养能够转化由通式（1）表示的含硫氨基醇化合物的微生物（其中R < SP POS =“POST”> 1 是氢，1-8C烷基或6-20C芳基）加入到含有低级脂族醇的培养基中的相应的含硫α-氨基酸化合物中以制备微生物细胞的微生物; 以及第二步骤，使含硫氨基醇化合物与第一步中获得的微生物的微生物细胞或微生物细胞的加工产物反应。版权所有（C）2012，JPO＆INPIT

4. 发明专利

JP2011188802A Mutant reductase 有权
标题翻译： MUTANT REDUCTASE
公开(公告)号：JP2011188802A
公开(公告)日：2011-09-29
申请号：JP2010057489
申请日：2010-03-15
申请人： Sumitomo Chemical Co Ltd , 住友化学株式会社
发明人： ASAKO HIROYUKI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12P7/62
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a reductase having excellent thermostability in order to shorten the reaction time and to improve reaction efficiency in a reduction reaction with an industrial useful reductase so as to produce an optically active compound, an intermediate thereof, etc. SOLUTION: The reductase includes the same amino acid sequence as an amino acid sequence composed of a specific sequence except that the reductase has one or more amino acid mutations containing an amino acid mutation in which 42 histidine is replaced with leucine in the amino acid sequence composed of the specific sequence (with the proviso that no amino acid mutations of an amino acid mutation in which 56 glycine is replaced with cysteine, an amino acid mutation in which 115 valine is replaced with isoleucine and an amino acid mutation in which 27 tryptophan is replaced with arginine are contained) and has capability of reducing a substrate. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：为了提供具有优异的热稳定性的还原酶以缩短反应时间并提高与工业有用的还原酶的还原反应中的反应效率，以便制备光学活性化合物，其中间体等。解决方案：还原酶包括与由特异性序列组成的氨基酸序列相同的氨基酸序列，除了还原酶具有一个或多个含有氨基酸突变的氨基酸突变，其中42个组氨酸被亮氨酸取代由特定序列组成的氨基酸序列（条件是没有氨基酸突变，其中56个甘氨酸被半胱氨酸取代的氨基酸突变，其中115个缬氨酸被异亮氨酸取代的氨基酸突变和氨基酸突变，其中 27个色氨酸被精氨酸所取代），具有降低底物的能力。版权所有（C）2011，JPO＆INPIT

5. 发明专利

JP2010115163A Method for obtaining reductase gene and reductase gene 有权
标题翻译：获得还原基因和降低基因的方法
公开(公告)号：JP2010115163A
公开(公告)日：2010-05-27
申请号：JP2008291407
申请日：2008-11-13
申请人： Shinya Ito , Sumitomo Chemical Co Ltd , 伸哉伊藤 , 住友化学株式会社
发明人： ITO SHINYA , MAKINO YOSHITSUGU , ASAKO HIROYUKI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12P7/02
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a method for obtaining a gene encoding a reductase.
SOLUTION: The method for obtaining a reductase gene includes (1) a process for using a primer set consisting of a nucleic acid fragment composed of a specific base sequence and a nucleic acid fragment composed of another specific base sequence, and performing a nucleic acid amplification reaction using a DNA library as a template to obtain an amplification product, (2) a process for inserting the amplification product to a vector to be connected to a functional promoter in a functionable form in a host cell to construct a recombinant vector, (3) a process for transferring the recombinant vector to the host cell to prepare a transformant and (4) a process for using the transformant or a treated material thereof to detect that the transformant or the treated material thereof has activity of asymmetrically reducing a carbonyl compound into an optically active alcohol.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供一种获得编码还原酶的基因的方法。解决方案：获得还原酶基因的方法包括（1）使用由特定碱基序列构成的核酸片段和由另一特异碱基序列构成的核酸片段组成的引物组的方法，使用DNA文库作为模板的核酸扩增反应以获得扩增产物，（2）将扩增产物插入到宿主细胞中以功能性形式连接到功能性启动子的载体以构建重组载体的方法，（3）将重组载体转移到宿主细胞以制备转化体的方法，和（4）使用转化体或其处理材料的方法来检测转化体或其处理物质具有不对称降低的活性羰基化合物转化为光学活性醇。版权所有（C）2010，JPO＆INPIT

6. 发明专利

JP2007159501A Method for producing sulfur-containing hydroxycarboxylic acid 有权
标题翻译：生产含硫酸羟基羧酸的方法
公开(公告)号：JP2007159501A
公开(公告)日：2007-06-28
申请号：JP2005361488
申请日：2005-12-15
申请人： Sumitomo Chemical Co Ltd , 住友化学株式会社
发明人： ASAKO HIROYUKI
IPC分类号： C12P11/00
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a sulfur-containing α-hydroxycarboxylic acid compound without the formation of a large amount of by-products or inhibition of an enzyme activity. SOLUTION: The method for producing the sulfur-containing α-hydroxycarboxylic acid compound represented by formula (2) (wherein, R 1 is a hydrogen, a 1-8C alkyl group or a 6-20C aryl group) is carried out as follows. Rhodococcus rhodochrous or a microbial cell of a microorganism belonging to the Rhodococcus sp. or a treated material of the microbial cell having an ability to convert a sulfur-containing dihydroxy compound represented by formula (1) äwherein, R 1 is the same as in formula (2)} into the corresponding α-hydroxycarboxylic acid compound and cultured in the presence of a primary or a secondary alcohol is made to act on the sulfur-containing dihydroxy compound. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供一种生产含硫α-羟基羧酸化合物而不形成大量副产物或抑制酶活性的方法。解决方案：制备由式（2）表示的含硫α-羟基羧酸化合物的方法（其中R 1为氢，1-8C烷基或6- 20C芳基）如下进行。玫瑰色红球菌或属于红球菌属的微生物的微生物细胞。或具有将式（1）表示的含硫二羟基化合物转化成相应α的能力的微生物细胞处理材料，其中R 1 与式（2）中的相同） - 羟基羧酸化合物并在伯或仲醇存在下培养，作用于含硫二羟基化合物。版权所有（C）2007，JPO＆INPIT

7. 发明专利

JP2011200182A Method for producing 5-(aminomethyl)-2-chlorothiazole 审中-公开
标题翻译：生产5-（氨基乙基）-2-氯代噻唑的方法
公开(公告)号：JP2011200182A
公开(公告)日：2011-10-13
申请号：JP2010071808
申请日：2010-03-26
申请人： Sumitomo Chemical Co Ltd , 住友化学株式会社
发明人： ASAKO HIROYUKI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P17/14
CPC分类号： C12P17/14 , C12Y305/01
摘要： PROBLEM TO BE SOLVED: To provide a method etc., for producing 5-(aminomethyl)-2-chlorothiazole useful as a synthetic intermediate.SOLUTION: The method etc., for producing the 5-(aminomethyl)-2-chlorothiazole include a reaction step of reacting an N-carbamoylamino compound represented by formula (2) with a culture product of a transformant obtained by introducing a polynucleotide having a base sequence encoding an amino acid sequence of an enzyme having an ability to convert the N-carbamoylamino compound into the corresponding 5-(aminomethyl)-2-chlorothiazole into a microbial cell or a treated substance thereof.
摘要翻译：要解决的问题：提供一种用于制备用作合成中间体的5-（氨基甲基）-2-氯噻唑的方法等。溶液：用于制备5-（氨基甲基）-2-氯噻唑的方法等包括使由式（2）表示的N-氨基甲酰氨基化合物与通过引入具有编码具有将N-氨基甲酰氨基化合物转化能力的酶的氨基酸序列的碱基序列的多核苷酸的多核苷酸的培养产物反应的反应步骤将相应的5-（氨基甲基）-2-氯噻唑转化成微生物细胞或其处理物质。

8. 发明专利

JP2012184215A Method for producing methionine 审中-公开
标题翻译：甲磺酸生产方法
公开(公告)号：JP2012184215A
公开(公告)日：2012-09-27
申请号：JP2011113182
申请日：2011-05-20
申请人： Sumitomo Chemical Co Ltd , 住友化学株式会社
发明人： HAGITANI KOJU , ASAKO HIROYUKI
IPC分类号： C07C319/20 , C07B61/00 , C07C319/18 , C07C323/25 , C07C323/58 , C12N1/20 , C12P13/12
CPC分类号： C07C319/20 , C07C319/18 , C12N1/32 , C12P13/12 , C07C323/25 , C07C323/58
摘要： PROBLEM TO BE SOLVED: To provide a novel method for producing methionine without using sodium cyanide as a raw material.SOLUTION: The method for producing methionine comprises a first step for reacting 2-amino-3-buten-1-ol with methanethiol and a second step for oxidizing 2-amino-4-methylthio-1-butanol obtained in the first step. In the first step, 2-amino-3-buten-1-ol is reacted with methanethiol preferably in the presence of a radical initiator.
摘要翻译：要解决的问题：提供一种不使用氰化钠作为原料生产甲硫氨酸的新方法。解决方案：产生甲硫氨酸的方法包括使2-氨基-3-丁烯-1-醇与甲硫醇反应的第一步骤和用于氧化在第一步中获得的2-氨基-4-甲硫基-1-丁醇的第二步骤步。在第一步中，优选在自由基引发剂存在下，使2-氨基-3-丁烯-1-醇与甲硫醇反应。版权所有（C）2012，JPO＆INPIT

9. 发明专利

JP2011188803A Mutant reductase 有权
标题翻译： MUTANT REDUCTASE
公开(公告)号：JP2011188803A
公开(公告)日：2011-09-29
申请号：JP2010057490
申请日：2010-03-15
申请人： Sumitomo Chemical Co Ltd , 住友化学株式会社
发明人： ASAKO HIROYUKI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12P7/62
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a reductase having excellent thermostability in order to shorten the reaction time and to improve reaction efficiency in a reduction reaction with an industrial useful reductase so as to produce an optically active compound, an intermediate thereof, etc. SOLUTION: The reductase includes the same amino acid sequence as an amino acid sequence composed of a specific sequence except that the reductase has one or more amino acid mutations containing an amino acid mutation in which 25 threonine is replaced with serine in the amino acid sequence composed of the specific sequence (with the proviso that no amino acid mutations of an amino acid mutation in which 56 glycine is replaced with cysteine, an amino acid mutation in which 115 valine is replaced with isoleucine, an amino acid mutation in which 27 tryptophan is replaced with arginine and an amino acid mutation in which 42 histidine is replaced with leucine are contained) and has capability of reducing a substrate. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：为了提供具有优异的热稳定性的还原酶以缩短反应时间并提高与工业有用的还原酶的还原反应中的反应效率，以便制备光学活性化合物，其中间体等。解决方案：还原酶包括与由特异性序列组成的氨基酸序列相同的氨基酸序列，除了还原酶具有一个或多个含有氨基酸突变的氨基酸突变，其中25个苏氨酸被丝氨酸替代由具体序列组成的氨基酸序列（条件是其中不存在56个甘氨酸被半胱氨酸取代的氨基酸突变的氨基酸突变，其中115个缬氨酸被异亮氨酸取代的氨基酸突变，其中氨基酸突变 27个色氨酸被精氨酸代替，其中含有42个组氨酸被亮氨酸取代的氨基酸突变），并具有还原能力 g底物。版权所有（C）2011，JPO＆INPIT

10. 发明专利

JP2011188800A Mutant reductase 有权
标题翻译： MUTANT REDUCTASE
公开(公告)号：JP2011188800A
公开(公告)日：2011-09-29
申请号：JP2010057487
申请日：2010-03-15
申请人： Sumitomo Chemical Co Ltd , 住友化学株式会社
发明人： ASAKO HIROYUKI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12P7/62
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a reductase that is industrially used in producing an optically active compound, an intermediate thereof, etc., and has excellent thermostability. SOLUTION: The reductase is derived from a strain of Yamadazyma farinosa IFO0193, has a specific amino acid sequence and has capability of reducing 2-oxo-4-phenylbutyric acid ester to (R)-2-hydroxy-4-phenylbutyric acid ester. COPYRIGHT: (C)2011,JPO&INPIT

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