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    • 2. 发明授权
    • Method of preparing microsphere composite of collagen and bioceramic powder
    • 胶原和生物陶瓷粉末微球复合材料的制备方法
    • US06752938B2
    • 2004-06-22
    • US09977591
    • 2001-10-13
    • Yng-Jiin WangShiao-Wen TsaiHsiu-Hsuan HuangWen Chung Chang
    • Yng-Jiin WangShiao-Wen TsaiHsiu-Hsuan HuangWen Chung Chang
    • B29B900
    • A61L27/48A61L27/34A61L27/46C08L5/08C08L5/04C08L89/06
    • This invention provides a method of preparing microsphere composite of collagen and bioceramic powder. This method first mixes a collagen solution with bioceramic powder and alginate, and squeezes the mixture solution to spherical droplets being discharged into a divalent cation solution undergoing gelling to form microspheres. Next, the microsphere is coated with a chitosan solution. Then, interior alginate and surface chitosan of the microsphere are liquefied and washed out with an aqueous buffer solution such as phosphate based buffer, and collagen in the microsphere is reconstituted to fiber network at the same time. The prepared microsphere composite has similar composition components of bone tissue, and the collagen thereof has a network of reconstituted fibers. The microsphere composite of this invention provides a similar growth environment of bone tissue cells, is used as a carrier to carry cells, coat and fix bone growth factors, and is applied in bone repair.
    • 本发明提供一种制备胶原和生物陶瓷粉末的微球复合材料的方法。 该方法首先将胶原溶液与生物陶瓷粉末和藻酸盐混合,并将混合物溶液挤压成球形小滴,将其排出成经历凝胶化的二价阳离子溶液以形成微球体。 接着,将微球涂覆有壳聚糖溶液。 然后,将微球的内部藻酸盐和表面壳聚糖液化并用缓冲液等缓冲溶液洗涤,同时将微球中的胶原重构成纤维网。 制备的微球复合材料具有相似的骨组织组成成分,其胶原蛋白具有复原纤维网。 本发明的微球复合材料提供了类似的骨组织细胞生长环境,用作携带细胞,涂覆和固定骨生长因子的载体,并用于骨修复。