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2. 发明专利

JP2005278549A Gene for producing 2-pyrone-4,6-dicarboxylic acid by fermentation, plasmid containing gene, transformant containing plasmid and method for producing 2-pyrone-4,6-dicarboxylic acid 有权
标题翻译：通过发酵生产2-吡咯烷-4,6-二羧酸的基因，含有基质的PLASMID，含有变性的等离子体和用于生产2-吡咯烷-4,6-二羧酸的方法
公开(公告)号：JP2005278549A
公开(公告)日：2005-10-13
申请号：JP2004099240
申请日：2004-03-30
申请人： Masao Fukuda , Akiyoshi Kameoka , Yoshihiro Katayama , Eiji Masai , 昭義亀岡 , 英司政井 , 義博片山 , 雅夫福田
发明人： KATAYAMA YOSHIHIRO , MASAI EIJI , FUKUDA MASAO , KAMEOKA AKIYOSHI
IPC分类号： C12N15/09 , C07K7/08 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P7/44
CPC分类号： Y02P20/544
摘要： PROBLEM TO BE SOLVED: To provide a new fermentation production technology for converting a low molecular plant-originated low molecular mixture containing vanillin, syringaldehyde, vanillic acid, syringic acid and protocatechuic acid produced by a low molecule-forming technology such as a chemical decomposition method such as hydrolysis, oxidative decomposition, solvolysis, etc., or physicochemical decomposition utilizing supercritical water and supercritical organic solvent of a plant aromatic component having various chemical structures and complex high molecular structures, through the multiple stages of enzymatic reactions to a single intermediate substance, 2-pyrone-4,6-dicarboxylic acid (PDC) in a high yield by designing and constructing with a recombinant DNA technology. SOLUTION: The gene sequences encoding 4 kinds of enzymes (benzaldehyde dehydrogenase, demethylase, protocatechuate 4,5-dioxygenase and 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase), and a new double strand DNA molecule obtained by joining gene domains associated with the regulation of their expressions are provided. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种新的发酵生产技术，用于转化低分子植物来源的低分子量混合物，其含有通过低分子形成技术产生的香草醛，丁香醛，香草酸，丁香酸和原儿茶酸，例如化学分解方法如水解，氧化分解，溶剂分解等，或利用具有各种化学结构和复杂高分子结构的植物芳香组分的超临界水和超临界有机溶剂进行物理化学分解，通过多次酶反应中间体，2-吡喃酮-4,6-二羧酸（PDC），以重组DNA技术设计和构建。解决方案：编码4种酶（苯甲醛脱氢酶，脱甲基酶，原儿茶酸4,5-双加氧酶和4-羧基-2-羟基木聚糖-6 - 半醛脱氢酶）的基因序列和通过连接获得的新型双链DNA分子提供了与其表达调节相关的基因结构域。版权所有（C）2006，JPO＆NCIPI

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