专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07476501B2 Methods and device for DNA sequencing using surface enhanced raman scattering (SERS) 失效
标题翻译：使用表面增强拉曼散射（SERS）进行DNA测序的方法和装置
公开(公告)号：US07476501B2
公开(公告)日：2009-01-13
申请号：US10108128
申请日：2002-03-26
申请人： Selena Chan , Xing Su , Tae-Woong Koo
发明人： Selena Chan , Xing Su , Tae-Woong Koo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q2565/60 , C12Q2565/631 , C12Q2565/632 , G01N21/658 , G01N33/587 , G01N2021/653 , G01N2021/656
摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, exonuclease treatment of the nucleic acids 109 results in the release of nucleotides 110. The nucleotides may pass from a reaction chamber 101 through a microfluidic channel 102 and enter a nanochannel or microchannel 103. The nanochannel or microchannel 103 may be packed with nanoparticle 111 aggregates containing hot spots for Raman detection. As the nucleotides 110 pass through the nanoparticle 111 hot spots, they may be detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Identification of the sequence of nucleotides 110 released from the nucleic acid 109 provides the nucleic acid sequence. Other embodiments of the invention concern apparatus 100 for nucleic acid sequencing.
摘要翻译：本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。在本发明的某些实施方案中，核酸109的核酸外切酶处理导致核苷酸110的释放。核苷酸可以从反应室101通过微流体通道102并进入纳米通道或微通道103.纳米通道或微通道103可以填充含有用于拉曼检测的热点的纳米颗粒111聚集体。当核苷酸110通过纳米颗粒111热点时，它们可以通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERRS）和/或相干抗斯托克斯拉曼光谱（CARS）来检测。从核酸109释放的核苷酸序列110的鉴定提供了核酸序列。本发明的其它实施方案涉及用于核酸测序的设备100。

2. 发明授权

US07105301B2 Detecting molecular binding by monitoring feedback controlled cantilever deflections 失效
标题翻译：通过监测反馈控制的悬臂偏转来检测分子结合
公开(公告)号：US07105301B2
公开(公告)日：2006-09-12
申请号：US10667776
申请日：2003-09-22
申请人： Xing Su , Selena Chan , Tae-Woong Koo , Mineo Yamakawa , Andrew A. Berlin
发明人： Xing Su , Selena Chan , Tae-Woong Koo , Mineo Yamakawa , Andrew A. Berlin
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/566 , G01N33/563
CPC分类号： C12Q1/6825 , B82Y5/00 , G01N33/54373 , G01N2800/52 , Y10S977/924 , C12Q2565/501
摘要： The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.
摘要翻译：本方法和装置涉及使用探针分子检测和/或鉴定目标分析物。在本发明的各种实施方案中，探针或分析物附着到一个或多个悬臂。将探针与分析物结合导致由检测单元检测到的悬臂的偏转。可以应用平衡力将悬臂恢复到其原始位置。平衡力可以是磁性的，电的或辐射的。生成平衡力的检测单元和机构可以可操作地耦合到诸如计算机的信息处理和控制单元。计算机可以通过平衡偏转力和平衡力来调节将悬臂维持在固定位置的反馈回路。样品中分析物的浓度可以从将悬臂维持在固定位置所需的平衡力的大小来确定。

3. 发明申请

US20060199193A1 Sensor arrays and nucleic acid sequencing applications 有权
公开(公告)号：US20060199193A1
公开(公告)日：2006-09-07
申请号：US11226696
申请日：2005-09-13
申请人： Tae-Woong Koo , Selena Chan , Xing Su , Zhang Jingwu , Mineo Yamakawa , Val Dubin
发明人： Tae-Woong Koo , Selena Chan , Xing Su , Zhang Jingwu , Mineo Yamakawa , Val Dubin
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6874 , B01J2219/00317 , B01J2219/00511 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00641 , B01J2219/00722 , B01L3/5085 , B82Y30/00 , C12Q1/6869 , G01N27/414 , G01N27/4145 , G01N27/4148
摘要： Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction cavities containing sensors to monitor changes in solutions contained in the reaction cavities. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction cavities that allow for optical monitoring of solutions in the reaction cavities. Test and fill reaction schemes are disclosed that allow DNA to be sequenced. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced.

4. 发明授权

US07019828B2 Chemical enhancement in surface enhanced raman scattering using lithium salts 失效
标题翻译：使用锂盐的表面增强拉曼散射的化学增强
公开(公告)号：US07019828B2
公开(公告)日：2006-03-28
申请号：US10387080
申请日：2003-03-12
申请人： Xing Su , Lei Sun , Tae-Woong Koo , Selena Chan
发明人： Xing Su , Lei Sun , Tae-Woong Koo , Selena Chan
IPC分类号： G01J3/44
CPC分类号： G01N21/658 , B82Y10/00 , B82Y20/00
摘要： Briefly, in accordance with one embodiment of the invention, the intensity of the signals from surface enhanced Raman spectroscopy may be increased by using lithium chloride as an enhancer to activate a metallic structure used for surface enhanced Raman spectroscopy. The increased signal intensity may allow surface enhanced Raman spectroscopy to be utilized to detect individual analytes such as nucleotides, for example in DNA sequencing without requiring a dye or radioactive label.
摘要翻译：简而言之，根据本发明的一个实施例，可以通过使用氯化锂作为增强剂激活用于表面增强拉曼光谱的金属结构来增加来自表面增强拉曼光谱的信号的强度。增加的信号强度可以允许使用表面增强拉曼光谱来检测单个分析物例如核苷酸，例如在DNA测序中，而不需要染料或放射性标记。

5. 发明授权

US09040237B2 Sensor arrays and nucleic acid sequencing applications 有权
标题翻译：传感器阵列和核酸测序应用
公开(公告)号：US09040237B2
公开(公告)日：2015-05-26
申请号：US11226696
申请日：2005-09-13
申请人： Tae-Woong Koo , Selena Chan , Xing Su , Zhang Jingwu , Mineo Yamakawa , Val M. Dubin
发明人： Tae-Woong Koo , Selena Chan , Xing Su , Zhang Jingwu , Mineo Yamakawa , Val M. Dubin
IPC分类号： C12Q1/68 , C12M1/00 , C12M3/00 , C12M1/34 , B82Y30/00 , B01L3/00 , G01N27/414
CPC分类号： C12Q1/6874 , B01J2219/00317 , B01J2219/00511 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00641 , B01J2219/00722 , B01L3/5085 , B82Y30/00 , C12Q1/6869 , G01N27/414 , G01N27/4145 , G01N27/4148
摘要： Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction cavities containing sensors to monitor changes in solutions contained in the reaction cavities. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction cavities that allow for optical monitoring of solutions in the reaction cavities. Test and fill reaction schemes are disclosed that allow DNA to be sequenced. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced.
摘要翻译：本发明的实施方案提供了使用包含传感器的反应腔阵列来测序DNA的装置方法，以监测包含在反应腔中的溶液的变化。另外的实施方案提供了使用允许对反应腔中的溶液进行光学监测的反应腔阵列对DNA进行测序的装置和方法。公开了允许DNA测序的测试和填充反应方案。通过使用大阵列中包含的平行反应测序DNA，可以快速测序DNA。

6. 发明授权

US07351591B2 Surface modification of metals for biomolecule detection using surface enhanced Raman scattering (SERS) 有权
公开(公告)号：US07351591B2
公开(公告)日：2008-04-01
申请号：US10814695
申请日：2004-03-30
申请人： Tae-Woong Koo , Selena Chan , Xing Su , Jingwu Zhang , Lei Sun
发明人： Tae-Woong Koo , Selena Chan , Xing Su , Jingwu Zhang , Lei Sun
IPC分类号： G01N33/553
CPC分类号： G01N21/658
摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

7. 发明申请

US20070155022A1 Degenerate binding detection and protein identification using Raman spectroscopy nanoparticle labels 审中-公开
标题翻译：使用拉曼光谱纳米颗粒标签的简并结合检测和蛋白质鉴定
公开(公告)号：US20070155022A1
公开(公告)日：2007-07-05
申请号：US11325833
申请日：2005-12-30
申请人： Mineo Yamakawa , Narayan Sundararajan , Andrew Berlin , Selena Chan , Xing Su , Tae-Woong Koo , Lei Sun , Kung-Bin Sung , Mark Roth
发明人： Mineo Yamakawa , Narayan Sundararajan , Andrew Berlin , Selena Chan , Xing Su , Tae-Woong Koo , Lei Sun , Kung-Bin Sung , Mark Roth
IPC分类号： G01N33/543 , C12M1/34
CPC分类号： G01N33/54373 , G01N21/658
摘要： Embodiments of the present invention provide methods for determining the degenerate binding capabilities of antibodies. The methods provide information about degenerate binding capabilities without the use of involved procedures. Optionally, a molecule toward which an antibody exhibits degenerate binding ability may be identified through the use of a reporter, such as, a composite organic inorganic nanocluster (COIN). COINs are sensitive SERS (surface enhanced Raman spectroscopy) reporters capable of multiplex analysis of analytes.
摘要翻译：本发明的实施方案提供了确定抗体的简并结合能力的方法。该方法提供了关于退化绑定功能的信息，而不使用涉及的过程。任选地，可以通过使用报告物如复合有机无机纳米簇（COIN）来鉴定抗体显示简并结合能力的分子。 COINs是敏感的SERS（表面增强拉曼光谱）报告人能够多分析分析物。

8. 发明申请

US20070059733A1 Method for sequencing nucleic acids by observing the uptake of nucleotides modified with bulky groups 审中-公开
标题翻译：通过观察用大体积改性的核苷酸摄取来测序核酸的方法
公开(公告)号：US20070059733A1
公开(公告)日：2007-03-15
申请号：US11445884
申请日：2006-06-02
申请人： Narayanan Sundararajan , Andrew Berlin , Mineo Yamakawa , Xing Su , Selena Chan , Tae-Woong Koo
发明人： Narayanan Sundararajan , Andrew Berlin , Mineo Yamakawa , Xing Su , Selena Chan , Tae-Woong Koo
IPC分类号： C12Q1/68 , G06F19/00 , C12M3/00
CPC分类号： C12Q1/6825 , B82Y5/00 , B82Y15/00 , B82Y30/00 , C12Q1/6869 , C12Q2563/155 , C12Q2565/607
摘要： The present methods and apparatus concern nucleic acid sequencing by incorporation of nucleotides into nucleic acid strands. The incorporation of nucleotides is detected by changes in the mass and/or surface stress of the structure. In some embodiments of the invention, the structure comprises one or more nanoscale or microscale cantilevers. In certain embodiments of the invention, each different type of nucleotide is distinguishably labeled with a bulky group and each incorporated nucleotide is identified by the changes in mass and/or surface stress of the structure upon incorporation of the nucleotide. In alternative embodiments of the invention only one type of nucleotide is exposed at a time to the nucleic acids. Changes in the properties of the structure may be detected by a variety of methods, such as piezoelectric detection, shifts in resonant frequency of the structure, and/or position sensitive photodetection.
摘要翻译：本发明的方法和装置涉及通过将核苷酸掺入核酸链而进行的核酸测序。通过结构的质量和/或表面应力的变化来检测核苷酸的掺入。在本发明的一些实施例中，该结构包括一个或多个纳米级或微尺寸悬臂。在本发明的某些实施方案中，每种不同类型的核苷酸可以用大体积区分地标记，并且每个掺入的核苷酸通过结合核苷酸时结构的质量和/或表面应力的变化来鉴定。在本发明的替代实施方案中，一次仅将一种类型的核苷酸暴露于核酸。可以通过各种方法来检测结构的性质的变化，例如压电检测，结构的谐振频率偏移和/或位置敏感的光电检测。

9. 发明申请

US20060134714A1 Detection and identification of peptide and protein modifications 审中-公开
标题翻译：肽和蛋白质修饰的检测和鉴定
公开(公告)号：US20060134714A1
公开(公告)日：2006-06-22
申请号：US11202862
申请日：2005-08-11
申请人： Narayan Sundararajan , Lei Sun , Xing Su , Mineo Yamakawa , Zhang Jingwu , Selena Chan , Andrew Berlin , Tae-Woong Koo , Mark Roth , Phil Gafken
发明人： Narayan Sundararajan , Lei Sun , Xing Su , Mineo Yamakawa , Zhang Jingwu , Selena Chan , Andrew Berlin , Tae-Woong Koo , Mark Roth , Phil Gafken
IPC分类号： C12Q1/48 , C12Q1/34
CPC分类号： G01N33/6851 , G01N21/658 , G01N33/54373 , G01N33/68 , G01N33/6842 , G01N33/6848 , Y02A90/26
摘要： Embodiments of the present invention provide devices and methods for detecting, identifying, distinguishing, and quantifying modification states of proteins and peptides using Surface Enhanced Raman (SERS) and Raman spectroscopy. Applications of embodiments of the present invention include, for example, proteome wide modification profiling and analyses with applications in disease diagnosis, prognosis and drug efficacy studies, enzymatic activity profiling and assays.
摘要翻译：本发明的实施方案提供了使用表面增强拉曼（SERS）和拉曼光谱法检测，鉴定，区分和定量蛋白质和肽的修饰状态的装置和方法。本发明的实施方案的应用包括例如蛋白质组广泛的修饰分析和分析，应用于疾病诊断，预后和药物疗效研究，酶活性分析和测定。

10. 发明申请

US20050221333A1 Microfluidic apparatus, systems, and methods for performing molecular reactions 有权
标题翻译：用于进行分子反应的微流体装置，系统和方法
公开(公告)号：US20050221333A1
公开(公告)日：2005-10-06
申请号：US10815264
申请日：2004-03-31
申请人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew Berlin
发明人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew Berlin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , G01N21/658 , Y10T436/11 , Y10T436/117497 , Y10T436/143333 , C12Q2565/629 , C12Q2521/319 , C12Q2565/632 , C12Q2565/619
摘要： Disclosed herein are methods, apparatuses, and systems for performing nucleic acid sequencing reactions and molecular binding reactions in a microfluidic channel. The methods, apparatuses, and systems can include a restriction barrier to restrict movement of a particle to which a nucleic acid is attached. Furthermore, the methods, apparatuses, and systems can include hydrodynamic focusing of a delivery flow. In addition, the methods, apparatuses, and systems can reduce non-specific interaction with a surface of the microfluidic channel by providing a protective flow between the surface and a delivery flow.
摘要翻译：本文公开了用于在微流体通道中进行核酸测序反应和分子结合反应的方法，装置和系统。方法，装置和系统可以包括限制核酸附着的颗粒运动的限制障碍。此外，方法，装置和系统可以包括输送流的流体动力聚焦。此外，方法，装置和系统可以通过在表面和输送流之间提供保护流来减少与微流体通道的表面的非特异性相互作用。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式