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1. 发明申请

US20100171950A1 METHODS FOR UNIFORM METAL IMPREGNATION INTO A NANOPOROUS MATERIAL 有权
标题翻译：将金属均匀地纳入纳米材料的方法
公开(公告)号：US20100171950A1
公开(公告)日：2010-07-08
申请号：US12575369
申请日：2009-10-07
申请人： Selena CHAN , Sunghoon Kwon , Narayan Sundararajan
发明人： Selena CHAN , Sunghoon Kwon , Narayan Sundararajan
IPC分类号： G01J3/44
CPC分类号： G01N21/658 , B01D67/0062 , B01D67/0086 , B01D67/0088 , B01D71/02 , B82Y15/00 , B82Y30/00 , C12Q1/6825 , G01J3/44 , G01N21/65 , G01N2021/6439 , G01N2021/655 , G01N2021/656 , C12Q2565/632 , C12Q2563/155
摘要： The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.
摘要翻译：本文公开的方法，系统400和装置涉及金属150浸渍的多孔基材110,210。本发明的某些实施方案涉及用于制造金属涂覆的多孔硅基板110,210的方法，其显示出显着改善金属150沉积物的均匀性和深度的渗透。增加的均匀性和深度允许分析物的改进和更可重复的拉曼检测。在本发明的示例性实施例中，所述方法可以包括多孔硅110的氧化，浸入金属盐溶液130中，干燥和热分解金属盐140以形成金属沉积物150.在本发明的其它示例性实施方案中，方法可以包括多孔硅衬底210与一种或多种金属盐溶液130的微流体浸渍。本发明的其它实施方案涉及用于分析物的拉曼检测的装置和/或系统400，包括金属涂覆的多孔硅衬底110,210，由公开的方法。

2. 发明授权

US07476786B2 Controlled alignment of nano-barcodes encoding specific information for scanning probe microscopy (SPM) reading 失效
标题翻译：用于扫描探针显微镜（SPM）读数的纳米条形码的控制对准编码特定信息
公开(公告)号：US07476786B2
公开(公告)日：2009-01-13
申请号：US10667004
申请日：2003-09-19
申请人： Selena Chan , Xing Su , Mineo Yamakawa
发明人： Selena Chan , Xing Su , Mineo Yamakawa
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/04 , A61K38/00 , C07K1/00
CPC分类号： C12Q1/6816 , B82Y5/00 , B82Y10/00 , B82Y30/00 , Y10S977/702 , Y10S977/704 , Y10S977/705 , Y10S977/728 , Y10S977/729 , Y10S977/734 , Y10S977/742 , Y10S977/773 , Y10S977/774 , Y10S977/924 , C12Q2565/601 , C12Q2563/185
摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nano-barcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nano-barcodes may be any molecule or complex that is distinguishable by scanning probe microscopy (SPM), such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and scanning probe microscopy (SPM) analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise a scanning probe microscopy (SPM) instrument and at least one coded probe attached to a surface.
摘要翻译：本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。在本发明的某些实施方案中，包含与一个或多个纳米条形码连接的探针分子的编码探针可以被允许与一个或多个靶分子结合。在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。纳米条形码可以是通过扫描探针显微镜（SPM）可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米粒子或量子点。当探针为寡核苷酸时，与靶核酸杂交的相邻编码探针可在对准和扫描探针显微镜（SPM）分析之前连接在一起。包含编码探针的组合物也在本文中公开。用于生物分子分析的系统可以包括扫描探针显微镜（SPM）仪器和连接到表面的至少一个编码探针。

3. 发明授权

US07476501B2 Methods and device for DNA sequencing using surface enhanced raman scattering (SERS) 失效
标题翻译：使用表面增强拉曼散射（SERS）进行DNA测序的方法和装置
公开(公告)号：US07476501B2
公开(公告)日：2009-01-13
申请号：US10108128
申请日：2002-03-26
申请人： Selena Chan , Xing Su , Tae-Woong Koo
发明人： Selena Chan , Xing Su , Tae-Woong Koo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q2565/60 , C12Q2565/631 , C12Q2565/632 , G01N21/658 , G01N33/587 , G01N2021/653 , G01N2021/656
摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, exonuclease treatment of the nucleic acids 109 results in the release of nucleotides 110. The nucleotides may pass from a reaction chamber 101 through a microfluidic channel 102 and enter a nanochannel or microchannel 103. The nanochannel or microchannel 103 may be packed with nanoparticle 111 aggregates containing hot spots for Raman detection. As the nucleotides 110 pass through the nanoparticle 111 hot spots, they may be detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Identification of the sequence of nucleotides 110 released from the nucleic acid 109 provides the nucleic acid sequence. Other embodiments of the invention concern apparatus 100 for nucleic acid sequencing.
摘要翻译：本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。在本发明的某些实施方案中，核酸109的核酸外切酶处理导致核苷酸110的释放。核苷酸可以从反应室101通过微流体通道102并进入纳米通道或微通道103.纳米通道或微通道103可以填充含有用于拉曼检测的热点的纳米颗粒111聚集体。当核苷酸110通过纳米颗粒111热点时，它们可以通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERRS）和/或相干抗斯托克斯拉曼光谱（CARS）来检测。从核酸109释放的核苷酸序列110的鉴定提供了核酸序列。本发明的其它实施方案涉及用于核酸测序的设备100。

4. 发明授权

US07361821B2 Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading 有权
标题翻译：用于扫描探针显微镜（SPM）读数的编码特定信息的纳米棒的受控比对
公开(公告)号：US07361821B2
公开(公告)日：2008-04-22
申请号：US10251152
申请日：2002-09-20
申请人： Selena Chan , Xing Su , Mineo Yamakawa
发明人： Selena Chan , Xing Su , Mineo Yamakawa
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6816 , B82Y5/00 , B82Y10/00 , B82Y30/00 , Y10S977/702 , Y10S977/704 , Y10S977/705 , Y10S977/728 , Y10S977/729 , Y10S977/734 , Y10S977/742 , Y10S977/773 , Y10S977/774 , Y10S977/924 , C12Q2565/601 , C12Q2563/185
摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nanobarcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nanobarcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise a scanning probe microscopy (SPM) instrument and at least one coded probe attached to a surface.
摘要翻译：本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。在本发明的某些实施方案中，包含连接至一个或多个纳米糖基的探针分子的编码探针可以被允许与一个或多个靶分子结合。在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。纳米线可以是通过SPM可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米颗粒或量子点。当探针是寡核苷酸时，与靶核酸杂交的相邻编码探针可以在对准和SPM分析之前连接在一起。包含编码探针的组合物也在本文中公开。用于生物分子分析的系统可以包括扫描探针显微镜（SPM）仪器和连接到表面的至少一个编码探针。

5. 发明授权

US07361313B2 Methods for uniform metal impregnation into a nanoporous material 有权
标题翻译：均匀金属浸渍到纳米多孔材料中的方法
公开(公告)号：US07361313B2
公开(公告)日：2008-04-22
申请号：US10368976
申请日：2003-02-18
申请人： Selena Chan , Sunghoon Kwon , Narayan Sundararajan
发明人： Selena Chan , Sunghoon Kwon , Narayan Sundararajan
IPC分类号： G01N21/65 , G01J3/44
CPC分类号： G01N21/658 , B01D67/0062 , B01D67/0086 , B01D67/0088 , B01D71/02 , B82Y15/00 , B82Y30/00 , C12Q1/6825 , G01J3/44 , G01N21/65 , G01N2021/6439 , G01N2021/655 , G01N2021/656 , C12Q2565/632 , C12Q2563/155
摘要： The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.
摘要翻译：本文公开的方法，系统400和装置涉及金属150浸渍的多孔基材110,210。本发明的某些实施方案涉及用于制造金属涂覆的多孔硅基板110,210的方法，其显示出显着改善金属150沉积物的均匀性和深度的渗透。增加的均匀性和深度允许分析物的改进和更可重复的拉曼检测。在本发明的示例性实施例中，所述方法可以包括多孔硅110的氧化，浸入金属盐溶液130中，干燥和热分解金属盐140以形成金属沉积物150.在本发明的其它示例性实施方案中，方法可以包括多孔硅衬底210与一种或多种金属盐溶液130的微流体浸渍。本发明的其它实施方案涉及用于分析物的拉曼检测的装置和/或系统400，包括金属涂覆的多孔硅衬底110,210，由公开的方法。

6. 发明授权

US07279134B2 Microfluidic devices with porous membranes for molecular sieving, metering, and separations 有权
标题翻译：具有用于分子筛，计量和分离的多孔膜的微流体装置
公开(公告)号：US07279134B2
公开(公告)日：2007-10-09
申请号：US10246049
申请日：2002-09-17
申请人： Selena Chan , Narayan Sundararajan , Andrew A. Berlin , Mineo Yamakawa
发明人： Selena Chan , Narayan Sundararajan , Andrew A. Berlin , Mineo Yamakawa
IPC分类号： B01L3/02 , B31D3/00
CPC分类号： B01D61/18 , B01D61/022 , B01D61/142 , B01D61/20 , B01D69/02 , B01D71/02 , B01D2325/02 , B01D2325/04 , B01L3/502753 , B01L2200/12 , B01L2300/0681 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： Microfluidic devices with porous membranes for molecular sieving, metering, and separation of analyte fluids. In one aspect, a microfluidic device includes a substrate having input and output microfluidic channel sections separated by a porous membrane formed integral to the substrate. In another aspect, the porous membrane may comprise a thin membrane that is sandwiched between upper and lower substrate members. The microfluidic device may include one or a plurality of porous membranes. In one embodiment, a plurality of porous membranes having increasingly smaller pores are disposed along portions of a microfluidic channel. In another embodiment, a cascading series of upper and lower channels are employed, wherein each upper/lower channel interface is separated by a respective porous membrane. In another aspect, a porous membrane is rotatably coupled to a substrate within a microfluidic channel via a MEMS actuator to enable the porous membrane to be positioned in filtering and pass-through positions.
摘要翻译：具有多孔膜的微流体装置用于分析筛分，计量和分析分析液体。在一个方面，微流体装置包括具有输入和输出微流体通道部分的基底，所述输入和输出微流体通道部分由与所述基底一体形成的多孔膜隔开。在另一方面，多孔膜可以包括夹在上部和下部衬底构件之间的薄膜。微流体装置可以包括一个或多个多孔膜。在一个实施例中，具有越来越小的孔的多个多孔膜沿着微流体通道的部分设置。在另一个实施例中，采用了上下通道的级联系列，其中每个上/下通道界面由相应的多孔膜隔开。在另一方面，多孔膜通过MEMS致动器可旋转地联接到微流体通道内的衬底，以使得多孔膜能够位于过滤位置和穿过位置。

7. 发明申请

US20070116882A1 Methods for uniform metal impregnation into a nanoporous material 有权
标题翻译：均匀金属浸渍到纳米多孔材料中的方法
公开(公告)号：US20070116882A1
公开(公告)日：2007-05-24
申请号：US11436489
申请日：2006-05-18
申请人： Selena Chan , Sunghoon Kwon , Narayan Sundararajan
发明人： Selena Chan , Sunghoon Kwon , Narayan Sundararajan
IPC分类号： B05D1/18
CPC分类号： G01N21/658 , B01D67/0062 , B01D67/0086 , B01D67/0088 , B01D71/02 , B82Y15/00 , B82Y30/00 , C12Q1/6825 , G01J3/44 , G01N21/65 , G01N2021/6439 , G01N2021/655 , G01N2021/656 , C12Q2565/632 , C12Q2563/155
摘要： The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.
摘要翻译：本文公开的方法，系统400和装置涉及金属150浸渍的多孔基材110,210。本发明的某些实施方案涉及用于制造金属涂覆的多孔硅基板110,210的方法，其显示出显着改善金属150沉积物的均匀性和深度的渗透。增加的均匀性和深度允许分析物的改进和更可重复的拉曼检测。在本发明的示例性实施例中，所述方法可以包括多孔硅110的氧化，浸入金属盐溶液130中，干燥和热分解金属盐140以形成金属沉积物150.在本发明的其它示例性实施方案中，方法可以包括多孔硅衬底210与一种或多种金属盐溶液130的微流体浸渍。本发明的其它实施方案涉及用于分析物的拉曼检测的装置和/或系统400，包括金属涂覆的多孔硅衬底110,210，由公开的方法。

8. 发明授权

US07105301B2 Detecting molecular binding by monitoring feedback controlled cantilever deflections 失效
标题翻译：通过监测反馈控制的悬臂偏转来检测分子结合
公开(公告)号：US07105301B2
公开(公告)日：2006-09-12
申请号：US10667776
申请日：2003-09-22
申请人： Xing Su , Selena Chan , Tae-Woong Koo , Mineo Yamakawa , Andrew A. Berlin
发明人： Xing Su , Selena Chan , Tae-Woong Koo , Mineo Yamakawa , Andrew A. Berlin
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/566 , G01N33/563
CPC分类号： C12Q1/6825 , B82Y5/00 , G01N33/54373 , G01N2800/52 , Y10S977/924 , C12Q2565/501
摘要： The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.
摘要翻译：本方法和装置涉及使用探针分子检测和/或鉴定目标分析物。在本发明的各种实施方案中，探针或分析物附着到一个或多个悬臂。将探针与分析物结合导致由检测单元检测到的悬臂的偏转。可以应用平衡力将悬臂恢复到其原始位置。平衡力可以是磁性的，电的或辐射的。生成平衡力的检测单元和机构可以可操作地耦合到诸如计算机的信息处理和控制单元。计算机可以通过平衡偏转力和平衡力来调节将悬臂维持在固定位置的反馈回路。样品中分析物的浓度可以从将悬臂维持在固定位置所需的平衡力的大小来确定。

9. 发明申请

US20060199193A1 Sensor arrays and nucleic acid sequencing applications 有权
公开(公告)号：US20060199193A1
公开(公告)日：2006-09-07
申请号：US11226696
申请日：2005-09-13
申请人： Tae-Woong Koo , Selena Chan , Xing Su , Zhang Jingwu , Mineo Yamakawa , Val Dubin
发明人： Tae-Woong Koo , Selena Chan , Xing Su , Zhang Jingwu , Mineo Yamakawa , Val Dubin
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6874 , B01J2219/00317 , B01J2219/00511 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00641 , B01J2219/00722 , B01L3/5085 , B82Y30/00 , C12Q1/6869 , G01N27/414 , G01N27/4145 , G01N27/4148
摘要： Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction cavities containing sensors to monitor changes in solutions contained in the reaction cavities. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction cavities that allow for optical monitoring of solutions in the reaction cavities. Test and fill reaction schemes are disclosed that allow DNA to be sequenced. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced.

10. 发明授权

US07019828B2 Chemical enhancement in surface enhanced raman scattering using lithium salts 失效
标题翻译：使用锂盐的表面增强拉曼散射的化学增强
公开(公告)号：US07019828B2
公开(公告)日：2006-03-28
申请号：US10387080
申请日：2003-03-12
申请人： Xing Su , Lei Sun , Tae-Woong Koo , Selena Chan
发明人： Xing Su , Lei Sun , Tae-Woong Koo , Selena Chan
IPC分类号： G01J3/44
CPC分类号： G01N21/658 , B82Y10/00 , B82Y20/00
摘要： Briefly, in accordance with one embodiment of the invention, the intensity of the signals from surface enhanced Raman spectroscopy may be increased by using lithium chloride as an enhancer to activate a metallic structure used for surface enhanced Raman spectroscopy. The increased signal intensity may allow surface enhanced Raman spectroscopy to be utilized to detect individual analytes such as nucleotides, for example in DNA sequencing without requiring a dye or radioactive label.
摘要翻译：简而言之，根据本发明的一个实施例，可以通过使用氯化锂作为增强剂激活用于表面增强拉曼光谱的金属结构来增加来自表面增强拉曼光谱的信号的强度。增加的信号强度可以允许使用表面增强拉曼光谱来检测单个分析物例如核苷酸，例如在DNA测序中，而不需要染料或放射性标记。

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