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1. 发明授权

US07238864B2 Acetolactate synthase gene promoter 有权
标题翻译：乙酰乳酸合酶基因启动子
公开(公告)号：US07238864B2
公开(公告)日：2007-07-03
申请号：US11075808
申请日：2005-03-10
申请人： Seiichi Toki , Hiroaki Ichikawa , Hidemitsu Nakamura , Kiyoshi Kawai , Koichiro Kaku , Tsutomu Shimizu
发明人： Seiichi Toki , Hiroaki Ichikawa , Hidemitsu Nakamura , Kiyoshi Kawai , Koichiro Kaku , Tsutomu Shimizu
IPC分类号： C07H21/04 , C12N5/05 , C12N15/09 , A01H1/00 , A01H5/00
CPC分类号： C12N15/8274 , C12N15/8222
摘要： The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5×SSC at 42° C.; The expression vector, also, includes a coding sequence located downstream of the promoter. Additionally, the method includes introducing the expression vector into a plant cell to obtain a transgenic plant; wherein the promoter activity is lower in mature seeds or root bases than in plant tissues other than mature seeds or root bases. The present invention also provides transformants containing a promoter; the promoter includes any of the DNAs (a) to (c) described above.
摘要翻译：本发明提供诱导组织特异性表达的方法，其包括制备具有启动子的表达载体。启动子包括以下DNA（a）至（c）中的任一种：（a）包含SEQ ID NO：1所示核苷酸序列的DNA; （b）DNA，其包含从SEQ ID NO：1的核苷酸1344至2843的核苷酸序列; 和（c）DNA，其是在严格条件下与包含与SEQ ID NO：1所示的核苷酸序列完全互补的核苷酸序列的DNA片段杂交的DNA片段，其中，（a），（b）或（ c）可以控制位于其下游的编码序列的转录; 并且其中严格条件在42℃为5×SSC; 表达载体也包括位于启动子下游的编码序列。此外，该方法包括将表达载体导入植物细胞以获得转基因植物; 其中成熟种子或根系中的启动子活性低于在成熟种子或根基以外的植物组织中的活性。本发明还提供含有启动子的转化体; 启动子包括上述DNA（a）至（c）中的任一种。

2. 发明申请

US20050241021A1 Acetolactate synthase gene promoter 有权
标题翻译：乙酰乳酸合酶基因启动子
公开(公告)号：US20050241021A1
公开(公告)日：2005-10-27
申请号：US11075808
申请日：2005-03-10
申请人： Seiichi Toki , Hiroaki Ichikawa , Hidemitsu Nakamura , Kiyoshi Kawai , Koichiro Kaku , Tsutomu Shimizu
发明人： Seiichi Toki , Hiroaki Ichikawa , Hidemitsu Nakamura , Kiyoshi Kawai , Koichiro Kaku , Tsutomu Shimizu
IPC分类号： A01H1/00 , A01H5/00 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/82
CPC分类号： C12N15/8274 , C12N15/8222
摘要： The present invention enables characteristic gene expression control and relates to a promoter, which comprises any one of the following DNAs (a) to (c): (a) a DNA, which comprises the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which comprises a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO: 1 by deletion, substitution, insertion, or addition of 1 or a plurality of nucleotides, and which can control transcription of a gene located downstream thereof; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment comprising a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1, and which can control the transcription of a gene located downstream thereof.
摘要翻译：本发明能够进行特征性基因表达控制，涉及包含以下DNA（a）〜（c）中的任一种的启动子：（a）包含SEQ ID NO：1所示的核苷酸序列的DNA。（b）DNA，其包含通过缺失，取代，插入或添加1个或多个核苷酸而衍生自SEQ ID NO：1所示核苷酸序列的核苷酸序列，并且其可以控制位于下游的基因的转录的; 和（c）DNA，其是可以在严格条件下与包含与SEQ ID NO：1所示的核苷酸序列互补的核苷酸序列的DNA片段杂交的DNA片段，其可以控制位于下游的基因的转录其中。

3. 发明申请

US20080213788A1 GENE CODING FOR SCYTALONE DEHYDRATASE EXHIBITING RESISTANCE TO AGRICULTURAL FUNGICIDAL AGENT 失效
标题翻译： SCYTALONE脱氢酶基因编码对农业防腐剂的抗性
公开(公告)号：US20080213788A1
公开(公告)日：2008-09-04
申请号：US12099631
申请日：2008-04-08
申请人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
发明人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
IPC分类号： C12Q1/68
CPC分类号： C12N9/88 , C12Q1/32 , G01N2500/00
摘要： The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi.The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.
摘要翻译：本发明提供了可广泛用于有关抗稻瘟病真菌的研究中的基因。该基因编码以下蛋白质（a）或（b）中的任一种：（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质; 或（b）通过缺失取代或添加一种或多种氨基酸组成的由SEQ ID NO：2所示的氨基酸序列组成的蛋白质，其在沙司酮脱水酶抑制剂的存在下显示出香叶醇脱水酶活性。

4. 发明授权

US08017400B2 Method for transformation using mutant acetolactate synthase gene 有权
标题翻译：使用突变乙酰乳酸合酶基因转化的方法
公开(公告)号：US08017400B2
公开(公告)日：2011-09-13
申请号：US11920161
申请日：2006-05-09
申请人： Kinya Toriyama , Ayako Okuzaki , Koichiro Kaku , Kiyoshi Kawai , Tsutomu Shimizu
发明人： Kinya Toriyama , Ayako Okuzaki , Koichiro Kaku , Kiyoshi Kawai , Tsutomu Shimizu
IPC分类号： C12N15/82
CPC分类号： C12N15/821 , C12N9/88 , C12N15/8209 , C12N15/8274
摘要： Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker.
摘要翻译：使用对PC除草剂具有高特异性的突变ALS基因有效地选择转化的细胞。转化方法包括以下步骤：用含有目的基因的重组载体和编码具有甘氨酸突变的突变乙酰乳酸合酶的基因转化宿主细胞，这相当于野生型乙酰乳酸合酶的氨基酸序列的位置95 从米到丙氨酸; 培养在前一步骤中获得的转化细胞在嘧啶基羧基除草剂存在下; 并且其中编码突变乙酰乳酸合酶的基因用作选择标记。

5. 发明申请

US20060223136A1 Scytalone dehydrogenase gene showing tolerance to agricultural pesticide 失效
标题翻译： Scytalone脱氢酶基因显示对农药的耐受性
公开(公告)号：US20060223136A1
公开(公告)日：2006-10-05
申请号：US10507132
申请日：2003-02-24
申请人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
发明人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
IPC分类号： C12Q1/18 , C07H21/04 , C12P21/06 , C12N1/16 , C12N9/04 , C12N15/74
CPC分类号： C12N9/88 , C12Q1/32 , G01N2500/00
摘要： The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.
摘要翻译：本发明提供了可广泛用于有关抗稻瘟病真菌的研究中的基因。该基因编码以下蛋白质（a）或（b）中的任一种：（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质; 或（b）通过缺失取代或添加一种或多种氨基酸组成的由SEQ ID NO：2所示的氨基酸序列组成的蛋白质，其在沙司酮脱水酶抑制剂的存在下显示出香叶醇脱水酶活性。

6. 发明授权

US08030547B2 Gene coding for acetolactate synthase 有权
标题翻译：乙酰乳酸合酶基因编码
公开(公告)号：US08030547B2
公开(公告)日：2011-10-04
申请号：US12053101
申请日：2008-03-21
申请人： Koichiro Kaku , Tsutomu Shimizu , Kiyoshi Kawai , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka
发明人： Koichiro Kaku , Tsutomu Shimizu , Kiyoshi Kawai , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka
IPC分类号： C12N15/29 , C12N15/82
CPC分类号： C12N9/88 , C12N15/8274 , C12N15/8278
摘要： Disclosed is a gene coding for a protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides. The protein (a) consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8, and the protein (b) consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.
摘要翻译：公开了编码对PC除草剂或磺酰脲除草剂具有高水平抗性的蛋白质（a）或（b）的基因。蛋白质（a）由SEQ ID NO：2,4,6和8中任一项的氨基酸序列组成，蛋白质（b）由SEQ ID NO：2,4,6,8所示氨基酸序列衍生的氨基酸序列组成通过取代，缺失或添加至少一个或多个氨基酸，ID NOS：2,4,6和8具有对嘧啶基羧基除草剂的抗性，并具有乙酰乳酸合酶活性。

7. 发明申请

US20050283855A1 Genes encoding acetolactate synthase 审中-公开
标题翻译：编码乙酰乳酸合酶的基因
公开(公告)号：US20050283855A1
公开(公告)日：2005-12-22
申请号：US10509121
申请日：2003-02-21
申请人： Koichiro Kaku , Tsutomu Shimizu , Kiyoshi Kawai , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka
发明人： Koichiro Kaku , Tsutomu Shimizu , Kiyoshi Kawai , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka
IPC分类号： C12N9/88 , C12N15/29 , C12N15/60 , C12N15/82 , A01H5/00 , C07K14/415
CPC分类号： C12N9/88 , C12N15/8274 , C12N15/8278
摘要： The present invention provides a gene coding for the following protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides: (a) a protein which consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8; (b) a protein which consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.
摘要翻译：本发明提供编码下列蛋白质的基因（a）或（b），其表现出对PC除草剂或磺酰脲除草剂的高水平抗性：（a）由SEQ ID NOS中任一个氨基酸序列组成的蛋白质：2,4,6和8; （b）通过取代，缺失或添加至少一个或多个氨基酸由SEQ ID NO：2,4,6和8中任一个氨基酸序列衍生的氨基酸序列组成的蛋白质具有抗性至嘧啶基羧基除草剂，并具有乙酰乳酸合成酶活性。

8. 发明申请

US20090217420A1 Method for transformation using mutant acetolactate synthase gene 有权
标题翻译：使用突变乙酰乳酸合酶基因转化的方法
公开(公告)号：US20090217420A1
公开(公告)日：2009-08-27
申请号：US11920161
申请日：2006-05-09
申请人： Kinya Toriyama , Ayako Okuzaki , Koichiro Kaku , Kiyoshi Kawai , Tsutomu Shimizu
发明人： Kinya Toriyama , Ayako Okuzaki , Koichiro Kaku , Kiyoshi Kawai , Tsutomu Shimizu
IPC分类号： A01H1/00 , C12N15/87
CPC分类号： C12N15/821 , C12N9/88 , C12N15/8209 , C12N15/8274
摘要： Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker.
摘要翻译：使用对PC除草剂具有高特异性的突变ALS基因有效地选择转化的细胞。转化方法包括以下步骤：用含有目的基因的重组载体和编码具有甘氨酸突变的突变乙酰乳酸合酶的基因转化宿主细胞，这相当于野生型乙酰乳酸合酶的氨基酸序列的位置95 从米到丙氨酸; 培养在前一步骤中获得的转化细胞在嘧啶基羧基除草剂存在下; 并且其中编码突变乙酰乳酸合酶的基因用作选择标记。

9. 发明授权

US07504203B2 Method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor 失效
标题翻译：评估稻瘟病真菌对水杨酸脱水酶抑制剂敏感性的方法
公开(公告)号：US07504203B2
公开(公告)日：2009-03-17
申请号：US12099631
申请日：2008-04-08
申请人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
发明人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
IPC分类号： C12Q1/527 , C12N9/88 , C12N15/00 , C12N1/21 , C12N5/10 , C12N5/04 , C12P21/00 , C07H21/00 , C07K14/00
CPC分类号： C12N9/88 , C12Q1/32 , G01N2500/00
摘要： The present invention provides a method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor, comprising the steps of: (a) and (b), which (a) identifies an amino acid in an amino acid sequence of a scytalone dehydratase isolated from the rice blast fungus, where the amino acid corresponds to valine at position 75 of SEQ ID NO: 4; and (b) assesses the sensitivity of the rice blast fungus to the scytalone dehydratase inhibitor, where if the amino acid identified in step (a) is methionine, the sensitivity of the rice blast fungus to The scytalone dehydratase inhibitor is assessed to be lower than that of a wild-type rice blast fungus which endogenously produces the scytalone dehydratase of SEQ ID NO: 4.
摘要翻译：本发明提供了一种评价稻瘟病真菌对水杨醛脱水酶抑制剂敏感性的方法，其特征在于包括以下步骤：（a）和（b），其中（a）鉴定了水tal酮脱水酶的氨基酸序列中的氨基酸分离自稻瘟病菌，其中氨基酸对应于SEQ ID NO：4的位置75处的缬氨酸; （b）评估稻瘟病真菌对镰刀菌脱水酶抑制剂的敏感性，其中如果步骤（a）中鉴定的氨基酸是甲硫氨酸，则稻瘟病真菌对镰刀菌脱水酶抑制剂的敏感性被评估为低于内源性产生SEQ ID NO：4的水杨醛脱水酶的野生型稻瘟病菌的那些。

10. 发明授权

US07399625B2 Scytalone dehydrogenase gene showing tolerance to agricultural pesticide 失效
标题翻译： Scytalone脱氢酶基因显示对农药的耐受性
公开(公告)号：US07399625B2
公开(公告)日：2008-07-15
申请号：US10507132
申请日：2003-02-24
申请人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
发明人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
IPC分类号： C07H21/00 , C12N15/00 , C12N5/10 , C12N5/04 , C12N5/06 , C12N1/21 , C12N9/88 , C12P21/06 , C07K14/00
CPC分类号： C12N9/88 , C12Q1/32 , G01N2500/00
摘要： The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion, substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.
摘要翻译：本发明提供了可广泛用于有关抗稻瘟病真菌的研究中的基因。该基因编码以下蛋白质（a）或（b）中的任一种：（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质; 或（b）通过缺失，取代或添加一种或多种氨基酸由SEQ ID NO：2所示的氨基酸序列组成的蛋白质，其在沙司酮脱水酶抑制剂存在下显示出沙利酮脱水酶活性。

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