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1. 发明申请

US20080213788A1 GENE CODING FOR SCYTALONE DEHYDRATASE EXHIBITING RESISTANCE TO AGRICULTURAL FUNGICIDAL AGENT 失效
标题翻译： SCYTALONE脱氢酶基因编码对农业防腐剂的抗性
公开(公告)号：US20080213788A1
公开(公告)日：2008-09-04
申请号：US12099631
申请日：2008-04-08
申请人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
发明人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
IPC分类号： C12Q1/68
CPC分类号： C12N9/88 , C12Q1/32 , G01N2500/00
摘要： The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi.The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.
摘要翻译：本发明提供了可广泛用于有关抗稻瘟病真菌的研究中的基因。该基因编码以下蛋白质（a）或（b）中的任一种：（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质; 或（b）通过缺失取代或添加一种或多种氨基酸组成的由SEQ ID NO：2所示的氨基酸序列组成的蛋白质，其在沙司酮脱水酶抑制剂的存在下显示出香叶醇脱水酶活性。

2. 发明申请

US20060223136A1 Scytalone dehydrogenase gene showing tolerance to agricultural pesticide 失效
标题翻译： Scytalone脱氢酶基因显示对农药的耐受性
公开(公告)号：US20060223136A1
公开(公告)日：2006-10-05
申请号：US10507132
申请日：2003-02-24
申请人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
发明人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
IPC分类号： C12Q1/18 , C07H21/04 , C12P21/06 , C12N1/16 , C12N9/04 , C12N15/74
CPC分类号： C12N9/88 , C12Q1/32 , G01N2500/00
摘要： The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.
摘要翻译：本发明提供了可广泛用于有关抗稻瘟病真菌的研究中的基因。该基因编码以下蛋白质（a）或（b）中的任一种：（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质; 或（b）通过缺失取代或添加一种或多种氨基酸组成的由SEQ ID NO：2所示的氨基酸序列组成的蛋白质，其在沙司酮脱水酶抑制剂的存在下显示出香叶醇脱水酶活性。

3. 发明授权

US07504203B2 Method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor 失效
标题翻译：评估稻瘟病真菌对水杨酸脱水酶抑制剂敏感性的方法
公开(公告)号：US07504203B2
公开(公告)日：2009-03-17
申请号：US12099631
申请日：2008-04-08
申请人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
发明人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
IPC分类号： C12Q1/527 , C12N9/88 , C12N15/00 , C12N1/21 , C12N5/10 , C12N5/04 , C12P21/00 , C07H21/00 , C07K14/00
CPC分类号： C12N9/88 , C12Q1/32 , G01N2500/00
摘要： The present invention provides a method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor, comprising the steps of: (a) and (b), which (a) identifies an amino acid in an amino acid sequence of a scytalone dehydratase isolated from the rice blast fungus, where the amino acid corresponds to valine at position 75 of SEQ ID NO: 4; and (b) assesses the sensitivity of the rice blast fungus to the scytalone dehydratase inhibitor, where if the amino acid identified in step (a) is methionine, the sensitivity of the rice blast fungus to The scytalone dehydratase inhibitor is assessed to be lower than that of a wild-type rice blast fungus which endogenously produces the scytalone dehydratase of SEQ ID NO: 4.
摘要翻译：本发明提供了一种评价稻瘟病真菌对水杨醛脱水酶抑制剂敏感性的方法，其特征在于包括以下步骤：（a）和（b），其中（a）鉴定了水tal酮脱水酶的氨基酸序列中的氨基酸分离自稻瘟病菌，其中氨基酸对应于SEQ ID NO：4的位置75处的缬氨酸; （b）评估稻瘟病真菌对镰刀菌脱水酶抑制剂的敏感性，其中如果步骤（a）中鉴定的氨基酸是甲硫氨酸，则稻瘟病真菌对镰刀菌脱水酶抑制剂的敏感性被评估为低于内源性产生SEQ ID NO：4的水杨醛脱水酶的野生型稻瘟病菌的那些。

4. 发明授权

US07399625B2 Scytalone dehydrogenase gene showing tolerance to agricultural pesticide 失效
标题翻译： Scytalone脱氢酶基因显示对农药的耐受性
公开(公告)号：US07399625B2
公开(公告)日：2008-07-15
申请号：US10507132
申请日：2003-02-24
申请人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
发明人： Koichiro Kaku , Satoshi Watanabe , Kiyoshi Kawai , Tsutomu Shimizu , Kozo Nagayama
IPC分类号： C07H21/00 , C12N15/00 , C12N5/10 , C12N5/04 , C12N5/06 , C12N1/21 , C12N9/88 , C12P21/06 , C07K14/00
CPC分类号： C12N9/88 , C12Q1/32 , G01N2500/00
摘要： The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion, substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.
摘要翻译：本发明提供了可广泛用于有关抗稻瘟病真菌的研究中的基因。该基因编码以下蛋白质（a）或（b）中的任一种：（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质; 或（b）通过缺失，取代或添加一种或多种氨基酸由SEQ ID NO：2所示的氨基酸序列组成的蛋白质，其在沙司酮脱水酶抑制剂存在下显示出沙利酮脱水酶活性。

5. 发明授权

US08030547B2 Gene coding for acetolactate synthase 有权
标题翻译：乙酰乳酸合酶基因编码
公开(公告)号：US08030547B2
公开(公告)日：2011-10-04
申请号：US12053101
申请日：2008-03-21
申请人： Koichiro Kaku , Tsutomu Shimizu , Kiyoshi Kawai , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka
发明人： Koichiro Kaku , Tsutomu Shimizu , Kiyoshi Kawai , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka
IPC分类号： C12N15/29 , C12N15/82
CPC分类号： C12N9/88 , C12N15/8274 , C12N15/8278
摘要： Disclosed is a gene coding for a protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides. The protein (a) consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8, and the protein (b) consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.
摘要翻译：公开了编码对PC除草剂或磺酰脲除草剂具有高水平抗性的蛋白质（a）或（b）的基因。蛋白质（a）由SEQ ID NO：2,4,6和8中任一项的氨基酸序列组成，蛋白质（b）由SEQ ID NO：2,4,6,8所示氨基酸序列衍生的氨基酸序列组成通过取代，缺失或添加至少一个或多个氨基酸，ID NOS：2,4,6和8具有对嘧啶基羧基除草剂的抗性，并具有乙酰乳酸合酶活性。

6. 发明申请

US20050283855A1 Genes encoding acetolactate synthase 审中-公开
标题翻译：编码乙酰乳酸合酶的基因
公开(公告)号：US20050283855A1
公开(公告)日：2005-12-22
申请号：US10509121
申请日：2003-02-21
申请人： Koichiro Kaku , Tsutomu Shimizu , Kiyoshi Kawai , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka
发明人： Koichiro Kaku , Tsutomu Shimizu , Kiyoshi Kawai , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka
IPC分类号： C12N9/88 , C12N15/29 , C12N15/60 , C12N15/82 , A01H5/00 , C07K14/415
CPC分类号： C12N9/88 , C12N15/8274 , C12N15/8278
摘要： The present invention provides a gene coding for the following protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides: (a) a protein which consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8; (b) a protein which consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.
摘要翻译：本发明提供编码下列蛋白质的基因（a）或（b），其表现出对PC除草剂或磺酰脲除草剂的高水平抗性：（a）由SEQ ID NOS中任一个氨基酸序列组成的蛋白质：2,4,6和8; （b）通过取代，缺失或添加至少一个或多个氨基酸由SEQ ID NO：2,4,6和8中任一个氨基酸序列衍生的氨基酸序列组成的蛋白质具有抗性至嘧啶基羧基除草剂，并具有乙酰乳酸合成酶活性。

7. 发明授权

US07119256B2 Gene encoding acetolactate synthase 有权
标题翻译：基因编码乙酰乳酸合酶
公开(公告)号：US07119256B2
公开(公告)日：2006-10-10
申请号：US10432962
申请日：2001-11-16
申请人： Tsutomu Shimizu , Ishizue Nakayama , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka , Koichiro Kaku
发明人： Tsutomu Shimizu , Ishizue Nakayama , Kozo Nagayama , Atsunori Fukuda , Yoshiyuki Tanaka , Koichiro Kaku
IPC分类号： C12N15/29 , C12N15/82
CPC分类号： C12N15/8278 , C12N9/88 , C12Y202/01006
摘要： The present invention provides a gene encoding ALS protein which shows a extremely high level of resistance to pyrimidinyl carboxy herbicides. The gene of the present invention encodes an acetolactate synthase protein, for instance, according to SEQ ID NO:1, where the acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity.
摘要翻译：本发明提供编码ALS蛋白的基因，其显示出对嘧啶基羧基除草剂极高的抗性水平。本发明的基因编码例如根据SEQ ID NO：1的乙酰乳酸合酶蛋白，其中乙酰乳酸合酶蛋白对嘧啶基羧基除草剂具有抗性并具有乙酰乳酸合酶活性。

8. 发明授权

US07094592B2 Bacillus sp. D747 strain, plant disease controlling agents and insect pest controlling agents using the same and control method using the agents 有权
标题翻译：芽孢杆菌 D747菌株，植物病害防治剂和使用相同的害虫控制剂以及使用该试剂的对照方法
公开(公告)号：US07094592B2
公开(公告)日：2006-08-22
申请号：US10495432
申请日：2002-11-26
申请人： Satoshi Watanabe , Jun Toyoshima , Tsutomu Shimizu , Koji Yamaji , Kozo Nagayama , Hiroyuki Yano
发明人： Satoshi Watanabe , Jun Toyoshima , Tsutomu Shimizu , Koji Yamaji , Kozo Nagayama , Hiroyuki Yano
IPC分类号： C12N1/20 , C12N1/00 , A01N63/00
CPC分类号： C12R1/07 , A01N63/00 , C12N1/20
摘要： The present invention relates to a novel strain of Bacillus sp. D747 (deposited as FERM BP-8234) and methods for controlling plant diseases and insect pests, comprising administering cultures of Bacillus sp. D747 (including the viable bacteria) or viable bacteria isolated by culturing, on the plant parts such as roots, stems, leaves, seeds, and the like, or in the culture soil.
摘要翻译：本发明涉及一种新型芽孢杆菌菌株 D747（保藏为FERM BP-8234）和控制植物病虫害的方法，包括施用芽孢杆菌属的培养物。 D747（包括活细菌）或通过在植物部分如根，茎，叶，种子等上或在培养土中培养分离的活细菌。

9. 发明授权

US06750176B2 Wettable compositions for use in agriculture, preparation method therefor, and storage method therefor 有权
标题翻译：用于农业的可湿性组合物，其制备方法及其储存方法
公开(公告)号：US06750176B2
公开(公告)日：2004-06-15
申请号：US10265270
申请日：2002-10-07
申请人： Yuji Misumi , Katsushi Toyooka , Satoshi Watanabe , Tsutomu Shimizu , Kozo Nagayama
发明人： Yuji Misumi , Katsushi Toyooka , Satoshi Watanabe , Tsutomu Shimizu , Kozo Nagayama
IPC分类号： A01N6304
CPC分类号： A01N63/04 , A01N25/14 , A01N25/08 , A01N2300/00
摘要： The present invention provides a wettable composition for use in agriculture comprising microorganisms as an active ingredient, which can maintain a high survival rate of the same over long periods, and exhibit superior physical properties during applications, and provide a method for stably storing the wettable composition for use in agriculture for a long period of time. That is, the present invention corresponds to a wettable composition for use in agriculture comprising spores of filamentous fungi which are in the form of dried powders having a volume median diameter ranging from 2 &mgr;m to 10 &mgr;m, and having a ratio of (volume 90% diameter)/(volume 10% diameter;) of not more than 5.62, and an adsorbent having a water absorption ability in an amount of at least 1% by mass based on the total mass of the composition. The composition is prepared by pulverizing and mixing a mixture of spores of filamentous fungi, an adsorbent having a water absorption ability, and other additive components such as a surfactant, in an appropriate ratio of the same, by means of impact pulverization, and adjusting a moisture content so that the moisture content is less than 2.5% by mass based on the total mass of the composition. In addition, the composition is stored by packaging in a bag formed from a hermetic film.
摘要翻译：本发明提供了一种用于农业的可湿性组合物，其包含微生物作为活性成分，其可长期保持其高存活率，并且在使用期间表现出优异的物理性能，并提供稳定地储存可湿性组合物的方法长期用于农业。也就是说，本发明对应于一种用于农业的可湿性组合物，其包含丝状真菌的孢子，其为体积中值粒径为2μm至10μm的干燥粉末形式，并且具有（体积90％直径）/（体积10％直径）为5.62以下，吸水能力为相对于组合物总质量至少1质量％的吸附剂。组合物通过粉碎和混合丝状真菌的孢子，具有吸水能力的吸附剂和其它添加剂组分如表面活性剂的混合物，通过冲击粉碎以适当的比例粉碎和混合，并调节水分含量使得水分含量基于组合物的总质量小于2.5质量％。此外，组合物通过包装储存在由密封膜形成的袋中。

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