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1. 发明申请

US20130176563A1 Biopolymer Optical Analysis Device and Method 有权
标题翻译：生物聚合物光学分析装置及方法
公开(公告)号：US20130176563A1
公开(公告)日：2013-07-11
申请号：US13812801
申请日：2011-07-21
申请人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
发明人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
IPC分类号： G01J3/02
CPC分类号： G01J3/0267 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , G01N21/658 , G01N33/48721 , C12Q2565/631 , C12Q2565/632
摘要： The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 130b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a, 130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.
摘要翻译：本发明提供一种使用简单的装置结构分析具有优异机械稳定性，高空间分辨率和灵敏度的生物聚合物的特性的装置和方法。具体地，测量生物聚合物的拉曼散射光，并通过使用生物聚合物性质分析芯片（100a）分析形成生物聚合物的单体单元的性质，其特征在于包括：固体基材（110）; 设置在所述固体基板（110）中的至少一个纳米孔（120）; 以及设置在固体基板（110）上的一个或多个导电薄膜（130a，130b）。生物聚合物性质分析芯片（100a）的特征在于，导电薄膜（130a，130b）部分地设置在形成有纳米孔（120）的固体基板（110）上，并且已经渗透到纳米孔中的生物聚合物 120）通过外部光照射产生拉曼散射光。

2. 发明授权

US09562809B2 Biopolymer optical analysis device and method 有权
标题翻译：生物聚合物光学分析装置及方法
公开(公告)号：US09562809B2
公开(公告)日：2017-02-07
申请号：US13812801
申请日：2011-07-21
申请人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
发明人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
IPC分类号： G01J3/02 , B82Y5/00 , B82Y15/00 , G01N33/487 , G01N21/65
CPC分类号： G01J3/0267 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , G01N21/658 , G01N33/48721 , C12Q2565/631 , C12Q2565/632
摘要： The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 130b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a, 130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.
摘要翻译：本发明提供一种使用简单的装置结构分析具有优异机械稳定性，高空间分辨率和灵敏度的生物聚合物的特性的装置和方法。具体地，测量生物聚合物的拉曼散射光，并通过使用生物聚合物性质分析芯片（100a）分析形成生物聚合物的单体单元的性质，其特征在于包括：固体基材（110）; 设置在所述固体基板（110）中的至少一个纳米孔（120）; 以及设置在固体基板（110）上的一个或多个导电薄膜（130a，130b）。生物聚合物性质分析芯片（100a）的特征在于，导电薄膜（130a，130b）部分地设置在形成有纳米孔（120）的固体基板（110）上，并且已经渗透到纳米孔中的生物聚合物 120）通过外部光照射产生拉曼散射光。

3. 发明申请

US20120312083A1 BIOPOLYMER ANALYSIS METHOD, BIOPOLYMER ANALYZER, AND BIOPOLYMER ANALYSIS CHIP 审中-公开
标题翻译：生物聚合物分析方法，生物聚合物分析仪和生物分析仪分析
公开(公告)号：US20120312083A1
公开(公告)日：2012-12-13
申请号：US13579329
申请日：2011-02-23
申请人： Rena Akahori , Takashi Anazawa , Satoshi Ozawa , Yoshiaki Yazawa , Tomoyuki Sakai , Hideyuki Noda
发明人： Rena Akahori , Takashi Anazawa , Satoshi Ozawa , Yoshiaki Yazawa , Tomoyuki Sakai , Hideyuki Noda
IPC分类号： G01N33/48
CPC分类号： C12Q1/6869 , G01N21/6428 , G01N33/48721 , C12Q2565/631
摘要： There have been the following problems with sequence analysis using multiple nanopores: trapping a sample in the nanopores is not always 100% efficient and unnecessary time is spent to measure pores in which no sample has been trapped, resulting in low measurement efficiency. To address the problems, a labeling substance is boned to a sample, and the sample to which the labeling substance has been bonded is trapped in the nanopores. An apparatus for observing the labeling substance is used to observe the labeling substance and monitor whether or not the sample has been trapped in the nanopores. Measuring only nanopores in which the sample has been trapped allows the measurement efficiency to be improved.
摘要翻译：使用多个纳米孔的序列分析存在以下问题：在纳米孔中捕获样品并不总是100％的有效性，并且花费不需要的时间来测量没有样品被捕获的孔隙，导致低的测量效率。为了解决这些问题，将标记物质结合到样品中，并且标记物质已经结合的样品被捕获在纳米孔中。用于观察标记物质的装置用于观察标记物质并监测样品是否已被捕获在纳米孔中。仅测量样品已被捕获的纳米孔，可以提高测量效率。

4. 发明授权

US5938908A Capillary array electrophoresis system 失效
标题翻译：毛细管阵列电泳系统
公开(公告)号：US5938908A
公开(公告)日：1999-08-17
申请号：US847735
申请日：1997-04-22
申请人： Takashi Anazawa , Hideki Kambara , Satoshi Takahashi , Kazumichi Imai , Hisanori Nasu
发明人： Takashi Anazawa , Hideki Kambara , Satoshi Takahashi , Kazumichi Imai , Hisanori Nasu
IPC分类号： G01N21/64 , B01D57/02 , G01N27/447 , G01N35/00 , G01N27/26
CPC分类号： G01N27/44721 , G01N27/44782 , G01N2035/00237
摘要： A capillary electrophoresis system comprising a plurality of capillaries filled with a migration medium in which samples to which fluorephore labels are added migrate, a light source providing an excitation light exciting the fluorephore labels, a photo detector detecting a fluorescence radiated from the fluorephore labels, and light focusing means placed between the plurality of capillaries to which the excitation light is irradiated. The parts of the plurality of capillaries to which the excitation light is irradiated and the light focusing means are arranged in a plane shape. The excitation light is irradiated through the plurality of capillaries and the light focusing means, the capillaries and light focusing means alternatively placed in the parts where an excitation light is irradiated. The light focusing means consisting of cylindrical rod lenses. The axis of the cylindrical rod lenses is placed substantially parallel with the capillaries, which can detect the fluorescence from samples by irradiating a plurality of capillaries substantially at the same time in a batch without mechanical scanning of a plurality of capillaries or without optical scanning of a light beam, providing a highly sensitive detection of samples.
摘要翻译：一种毛细管电泳系统，包括填充有迁移介质的多个毛细管，其中添加有荧光标记的样品迁移，提供激发荧光标记的激发光的光源，检测从荧光标记物辐射的荧光的光电检测器，以及放置在激发光照射到的多个毛细管之间的光聚焦装置。照射激发光的多个毛细管的部分和光聚焦装置被布置成平面形状。激发光通过多个毛细管和光聚焦装置照射，毛细管和光聚焦装置交替地放置在照射激发光的部分中。光聚焦装置由圆柱形棒状透镜组成。圆柱形棒状透镜的轴线基本上与毛细管平行放置，其可以通过基本上同时照射多个毛细管而检测来自样品的荧光，而不需要对多个毛细管进行机械扫描，或者不进行光学扫描光束，提供高灵敏度的样品检测。

5. 发明授权

US5730850A Capillary array electrophoresis system 失效
标题翻译：毛细管阵列电泳系统
公开(公告)号：US5730850A
公开(公告)日：1998-03-24
申请号：US588776
申请日：1996-01-19
申请人： Hideki Kambara , Satoshi Takahashi , Takashi Anazawa , Takashi Yamada , Yoshinobu Kohara
发明人： Hideki Kambara , Satoshi Takahashi , Takashi Anazawa , Takashi Yamada , Yoshinobu Kohara
IPC分类号： G01N27/447 , G01N35/00 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44721 , G01N2035/00237
摘要： A capillary array electrophoresis system by which measurement is conducted using a large number of capillaries. The electrophoresis system includes a plurality of capillary array sheets stacked one on top of another wherein end portions of the capillaries at a detection region are arranged two-dimensionally in such a manner as to elute two-dimensionally a sample from the distal end of each capillary. Excitation light is applied to the sample eluted into a buffer solution, and a two-dimensional fluorescent image is picked up by a detector.
摘要翻译：使用大量毛细管进行测量的毛细管阵列电泳系统。所述电泳系统包括多个毛细管阵列片，所述多个毛细管阵列片层叠在另一个之上，其中毛细管在检测区域的端部以二维排列，从每个毛细管的末端二维地抽出样品。将激发光施加到洗脱到缓冲溶液中的样品上，并通过检测器拾取二维荧光图像。

6. 发明授权

US5968331A Sample holding device for electrophoresis apparatus and sample injection method 失效
标题翻译：电泳仪样品保持装置及样品注射法
公开(公告)号：US5968331A
公开(公告)日：1999-10-19
申请号：US554808
申请日：1995-11-07
申请人： Hideki Kambara , Satoshi Takahashi , Takashi Anazawa , Takashi Yamada
发明人： Hideki Kambara , Satoshi Takahashi , Takashi Anazawa , Takashi Yamada
IPC分类号： G01N33/50 , G01N27/447 , G01N27/26
CPC分类号： G01N27/44743
摘要： A sample holding device for electrophoresis apparatus according to the present invention wherein a plurality of sample holding capillaries are laid out in an array and are immobilized to the supporting jig. This sample holding device is configured to ensure the lower ends of the capillaries can contact the sample injection portion of the electrophoresis separation part of the electrophoresis apparatus, and provides easy sample injection and prevents the gel capillaries of the electrophoresis separation part from being damaged when sample holding capillaries are filled with gels, thereby allowing repeated use of the gel capillaries of the electrophoresis separation part.
摘要翻译：根据本发明的用于电泳装置的样品保持装置，其中多个样品保持毛细管以阵列布置并固定在支撑夹具上。该样品保持装置被配置为确保毛细血管的下端可以接触电泳装置的电泳分离部的样品注入部，并且提供容易的样品注射，并且防止当样品时电泳分离部分的凝胶毛细血管损伤保持毛细血管填充凝胶，从而允许重复使用电泳分离部分的凝胶毛细血管。

7. 发明授权

US5833827A Capillary array electrophoresis system 失效
标题翻译：毛细管阵列电泳系统
公开(公告)号：US5833827A
公开(公告)日：1998-11-10
申请号：US710894
申请日：1996-09-23
申请人： Takashi Anazawa , Satoshi Takahashi , Hideki Kambara
发明人： Takashi Anazawa , Satoshi Takahashi , Hideki Kambara
IPC分类号： G01N27/447 , G01N35/00 , G01N27/26
CPC分类号： G01N27/44721 , G01N27/44782 , G01N2035/00237
摘要： The capillary array electrophoresis system of the present invention comprises a plurality of capillaries whose inside is filled with migration medium and at least parts of which are aligned in a plane, a means for making lasers substantially simultaneously irradiate transparent parts of the plurality of capillaries along the direction of alignment of the plurality of capillaries so as to excite fluorephore labels of migrated and separated samples, a fluorescence detection means for detecting fluorescence radiating from the fluorephore labels, from a direction crossing the direction of the alignment, the transparent parts of the plurality of capillaries are surrounded by a transparent gas, the cross section of the capillary of the transparent part is a circle and the ratio of the outside diameter to the inside diameter of the capillary ranges from 1 to 7.
摘要翻译：本发明的毛细管阵列电泳系统包括多个毛细管，其内部填充有迁移介质，并且其至少一部分在平面中对准，用于使激光器基本上同时沿着多个毛细管的透明部分照射的装置沿着多个毛细管的排列方向，以激发迁移和分离的样品的荧光标记;荧光检测装置，用于检测从荧光标记物发出的荧光，从与取向方向相交的方向，多个毛细管被透明气体包围，透明部分的毛细管的横截面为圆形，毛细管的外径与内径之比为1〜7。

8. 发明授权

US5534703A Electrophoresis analyzer with wavelength selective detection 失效
标题翻译：具有波长选择性检测的电泳分析仪
公开(公告)号：US5534703A
公开(公告)日：1996-07-09
申请号：US220261
申请日：1994-03-30
申请人： Hideki Kambara , Takashi Anazawa , Satoshi Takahashi , Katsuhiko Murakami
发明人： Hideki Kambara , Takashi Anazawa , Satoshi Takahashi , Katsuhiko Murakami
IPC分类号： G01J3/427 , G01N21/27 , G01N21/64 , G01N27/447 , G01N27/26
CPC分类号： G01N21/6456 , G01N27/44721
摘要： An electrophoresis analyzer includes a light detector for detecting images of a linear fluorescence emitting region, the linear fluorescence emitting region including a plurality of fluorescence emitting points disposed along a straight line, each of the fluorescence emitting points being a point at which samples emit light including a plurality of wavelengths, the light detector including a line sensor, the line sensor including a plurality of photopixels disposed along a straight line, the photopixels constituting a detection surface of the line sensor, an image forming unit for receiving the light emitted by the samples at the fluorescence emitting points and forming a plurality of images of each of the fluorescence emitting points on the detection surface of the line sensor at mutually different positions disposed at a predetermined interval along the straight line along which the photopixels are disposed, and a filter unit disposed between the linear fluorescence emitting region and the light detector for selectively filtering the light emitted by the samples at the fluorescence emitting points such that the images of each of the fluorescence emitting points are formed by light including mutually different ones of the wavelengths.
摘要翻译：电泳分析仪包括用于检测线性荧光发射区域的图像的光检测器，所述线性荧光发射区域包括沿着直线设置的多个荧光发射点，每个荧光发射点是样品发射的点，包括多个波长，所述光检测器包括线传感器，所述线传感器包括沿着直线设置的多个照相像素，构成所述线传感器的检测表面的所述光像素，用于接收由所述样本发射的光的图像形成单元在荧光发射点处，并且沿着沿着设置有像素的直线以预定间隔设置的相互不同的位置，在线传感器的检测表面上形成每个荧光发射点的多个图像，以及滤光器单元设置在线性荧光发射区域a之间 nd光检测器，用于在荧光发射点选择性地过滤由样品发射的光，使得每个荧光发射点的图像由包括相互不同的波长的光形成。

9. 发明授权

US08040515B2 Fluorescence detection apparatus and method, and prism used therein 有权
标题翻译：荧光检测装置和方法以及其中使用的棱镜
公开(公告)号：US08040515B2
公开(公告)日：2011-10-18
申请号：US12051245
申请日：2008-03-19
申请人： Tsuyoshi Sonehara , Takashi Anazawa , Kenko Uchida , Tomoyuki Sakai
发明人： Tsuyoshi Sonehara , Takashi Anazawa , Kenko Uchida , Tomoyuki Sakai
IPC分类号： G01N21/25
CPC分类号： G01N21/6452 , G02B2207/113
摘要： In order to provide a fluorescence detection apparatus having a high sensitivity, a high processing capacity and a competitive edge in cost, the fluorescence detection apparatus according to this invention irradiate the sample with light so that the aspect ratio of the form of the irradiated region by light on the arrangement surface of the sample may be 1±0.1. The preferable form of irradiate region is not limited to one and varies to some extent depending on the item to be optimized. The form of irradiated region may be, for example, a circle, an equilateral triangle, a square, a regular hexagon and the like.
摘要翻译：为了提供具有高灵敏度，高处理能力和成本方面的竞争优势的荧光检测装置，根据本发明的荧光检测装置用光照射样品，使得照射区域的形式的纵横比由样品排列表面上的光可能为1±0.1。照射区域的优选形式不限于一个，并且根据要优化的项目而在一定程度上变化。照射区域的形式可以是例如圆形，等边三角形，正方形，正六边形等。

10. 发明授权

US06531044B1 Capillary array electrophoresis apparatus 有权
标题翻译：毛细管阵列电泳仪
公开(公告)号：US06531044B1
公开(公告)日：2003-03-11
申请号：US09425064
申请日：1999-10-25
申请人： Takashi Anazawa , Keiichi Nagai
发明人： Takashi Anazawa , Keiichi Nagai
IPC分类号： G01N2726
CPC分类号： G01N27/44782 , G01N27/44721
摘要： The electrophoresis apparatus includes plural capillaries for separating fluorephore-labeled samples by electrophoresis, fluorescence detecting parts provided in a part of these capillaries arranged in the same place for detecting a fluorescence emitted by fluorephore labels when a part of the plural capillaries is scanned and irradiated by a laser beam, and a fluorescence detection system for detecting this fluorescence. The fluorescence detecting parts are scanned and repeatedly irradiated by the laser bean where a scanning period of the fluorescence detecting parts by the laser bean is t1, and the fluorescence is detected by the fluorescence detecting system where an acquisition time of fluorescence signal is t2 (t1≦t2). The laser bean from a laser source is narrowly converged by a light collecting lens, and a galvanomirror is rotated in a rotation directional of the galvanomirror around the rotation axis of the galvanomirror so as to repeatedly scan the fluorescence detecting parts.
摘要翻译：电泳装置包括多个毛细管，用于通过电泳分离荧光标记的样品，荧光检测部分设置在这些毛细管的一部分中，布置在相同的位置，用于当多个毛细血管的一部分被扫描和照射时，用于检测由荧光标记物发出的荧光激光束和用于检测该荧光的荧光检测系统。荧光检测部件被激光束的荧光检测部的扫描期间为t1的激光束扫描并重复照射，荧光检测系统通过荧光检测系统检测荧光，其中荧光信号的获取时间为t2（t1 <= t2）。来自激光源的激光束由聚光透镜狭窄地收敛，并且电流镜以电流镜的旋转方向围绕电流镜的旋转轴线旋转，以便重复扫描荧光检测部分。

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