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1. 发明授权

US07183087B2 Process for preparing polyhydroxyalkanoate employing maoC gene 失效
标题翻译：使用maoC基因制备聚羟基链烷酸酯的方法
公开(公告)号：US07183087B2
公开(公告)日：2007-02-27
申请号：US10662358
申请日：2003-09-16
申请人： Sang Yup Lee , Si Jae Park
发明人： Sang Yup Lee , Si Jae Park
IPC分类号： C12P7/62 , C12N1/20 , C12N9/88 , C12N15/00 , C07H21/04
CPC分类号： C12N9/93 , C08G63/06 , C12N9/88 , C12P7/625 , C12Y402/01017
摘要： The present invention relates to a method for producing middle-chain-length polyhydroxyalkanoate (MCL-PHA) using a maoC gene. The producing method of MCL-PHA according to the present invention comprises the steps of transforming a microorganism with the maoC gene to give a transformant, the microorganism being deleted of a fadB gene and containing a PHA synthase gene; culturing the transformant in medium containing a C6-10 carbon source; and obtaining PHA consisting of monomers with 6–10 carbon atoms. When the maoC gene whose function has not yet been established is used according to the present invention, high quality PHA with a higher number of carbon atoms than the prior PHA can be produced at a higher efficiency.
摘要翻译：本发明涉及使用maoC基因生产中链长度聚羟基链烷酸酯（MCL-PHA）的方法。根据本发明的MCL-PHA的制备方法包括以下步骤：用maoC基因转化微生物以产生转化体，所述微生物缺失fadB基因并含有PHA合酶基因; 在含有C 6-10碳源的培养基中培养转化体; 并获得由具有6-10个碳原子的单体组成的PHA。当根据本发明使用尚未建立功能的maoC基因时，可以以更高的效率生产具有比先前的PHA高的碳原子数的高质量PHA。

2. 发明授权

US08883463B2 Recombinant microorganism having ability to produce [lactate-co-glycolate] copolymer from glucose, and method for preparing [lactate-co-glycolate] copolymer using same 有权
标题翻译：能够从葡萄糖生产[乳酸 - 共 - 乙醇酸]共聚物的能力的重组微生物以及使用其制备[乳酸 - 共 - 乙醇酸]共聚物的方法
公开(公告)号：US08883463B2
公开(公告)日：2014-11-11
申请号：US14004437
申请日：2012-03-09
申请人： Sang Yup Lee , Si Jae Park , Seung Hwan Lee , Bong Keun Song , Yu Kyung Jung , Tae Woo Lee
发明人： Sang Yup Lee , Si Jae Park , Seung Hwan Lee , Bong Keun Song , Yu Kyung Jung , Tae Woo Lee
IPC分类号： C12N15/74 , C12N5/04 , C12N1/20 , C12P7/62 , C12N15/70 , C12N9/04 , C12N9/10
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12N15/70
摘要： There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment.
摘要翻译：提供了一种具有来自葡萄糖的聚（乳酸 - 共 - 乙醇酸）的生产性的重组微生物，更具体而言，是在不添加外源乙醇酸酯前体的情况下具有聚（乳酸 - 共 - 乙醇酸）的生产性的重组微生物， [聚（制备乳酸 - 共 - 乙醇酸）]。根据本发明，可以高浓度制备其中乙醇酸酯部分浓度高的聚（乳酸 - 共 - 乙醇酸），而不提供外源乙醛酸酯。因此，本发明可以有效地用于治疗。

3. 发明授权

US08158387B2 Method for cell surface display of target proteins using FadL of E. coli 有权
标题翻译：使用大肠杆菌FadL对靶蛋白进行细胞表面显示的方法
公开(公告)号：US08158387B2
公开(公告)日：2012-04-17
申请号：US10588782
申请日：2004-08-30
申请人： Sang Yup Lee , Seung Hwan Lee , Si Jae Park
发明人： Sang Yup Lee , Seung Hwan Lee , Si Jae Park
IPC分类号： C12P21/04 , C12N1/21 , C12N15/00
CPC分类号： C12N15/70
摘要： The present invention relates to an expression vector which can effectively express target proteins or peptides on the surface of cells using an outer membrane protein (FadL) of E. coli as a surface anchoring motif. Also, the present invention relates to microorganisms transformed with the expression vector, and a method for stably expressing large amounts of target proteins on the surface of cells by culturing the transformed microorganisms. Furthermore, the present invention relates to a production method of protein arrays, a production method of antibodies, and a bioconversion method, the methods being characterized by using target proteins which have been expressed on the cell surface by the inventive method. In addition, the present invention relates to a method for improving target proteins by the inventive surface expression method. The present invention allows target proteins with normal functions to be expressed on an outer cell membrane. Thus, the present invention will be useful in recombinant live vaccines, the screening of various peptides or antibodies, whole-cell adsorbents for heavy metal removal or waste water treatment, whole-cell bioconversion, and the like.
摘要翻译：本发明涉及使用大肠杆菌的外膜蛋白（FadL）作为表面锚定基序，能够在细胞表面上有效表达靶蛋白或肽的表达载体。此外，本发明涉及用表达载体转化的微生物，以及通过培养转化的微生物在细胞表面上稳定表达大量靶蛋白的方法。此外，本发明涉及蛋白质阵列的制造方法，抗体的制造方法以及生物转化方法，其特征在于，使用通过本发明的方法在细胞表面表达的靶蛋白。此外，本发明涉及通过本发明的表面表达方法改善靶蛋白的方法。本发明允许具有正常功能的靶蛋白在外细胞膜上表达。因此，本发明可用于重组活疫苗，各种肽或抗体的筛选，用于重金属去除或废水处理的全细胞吸附剂，全细胞生物转化等。

4. 发明申请

US20110201067A1 METHOD FOR PREPARING POLYLACTATE AND COPOLYMER THEREOF USING A MUTANT MICROORGANISM WITH ENHANCED POLYLACTATE, AND THE COPOLYMER PRODUCING CAPABILITY THEREOF 有权
标题翻译：使用具有增强聚氨酯的突变微生物制备聚乙烯和共聚物的方法，以及其生产能力的共聚物
公开(公告)号：US20110201067A1
公开(公告)日：2011-08-18
申请号：US13001001
申请日：2009-06-24
申请人： Sang Yup Lee , Yu Kyung Jung , Taek Ho Yang , Si Jae Park , Tae Wan Kim
发明人： Sang Yup Lee , Yu Kyung Jung , Taek Ho Yang , Si Jae Park , Tae Wan Kim
IPC分类号： C12P7/62
CPC分类号： C08G63/06 , C12P7/625 , C12P21/02
摘要： Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.
摘要翻译：提供了使用突变微生物制备聚乳酸（PLA）或其共聚物的方法，其中遗传操作参与辅酶A（CoA）供体和乳酸生产途径的基因以提高CoA供体和乳酸的生产率。 CoA供体和乳酸盐的量在微生物代谢途径中同时增加，以有效生物合成PLA和具有高含量乳酸盐的羟基链烷酸酯 - 乳酸共聚物，其在工业上是有用的。

5. 发明授权

US07291325B2 Method for producing target proteins by deleting or amplifying ibpA and/or ibpB gene coding for inclusion body-associated proteins 失效
标题翻译：通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的方法
公开(公告)号：US07291325B2
公开(公告)日：2007-11-06
申请号：US10545849
申请日：2003-07-10
申请人： Sang Yup Lee , Mee Jung Han , Si Jae Park
发明人： Sang Yup Lee , Mee Jung Han , Si Jae Park
IPC分类号： A61K48/00 , A01N63/00 , C07H21/02 , C07H21/04
CPC分类号： C12P21/02 , C07K14/245 , C07K14/5434 , C07K14/57 , C07K14/65 , C12N15/70
摘要： A method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. Two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli are described. The first method enhances the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method enhances the production of target proteins in the cytoplasm and also converts the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.
摘要翻译：通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的方法。描述了使用编码大肠杆菌的包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的两种方法。第一种方法使用ibpA和/或ibpB基因缺失的细菌增强了靶蛋白的分泌生产和活性。第二种方法增强细胞质中靶蛋白的产生，并使用ibpA和/或ibpB基因扩增的细菌将靶蛋白从可溶形式转化为不溶性包涵体。

6. 发明授权

US07148334B2 Composition for protecting proteins degradation comprising small heat shock proteins (sHSPs) and method of two-dimensional gel electrophoresis using the sHSPs 失效
标题翻译：用于保护包含小热休克蛋白（sHSP）的蛋白质降解的组合物和使用sHSPs的二维凝胶电泳方法
公开(公告)号：US07148334B2
公开(公告)日：2006-12-12
申请号：US10791059
申请日：2004-03-02
申请人： Sang Yup Lee , Mee-Jung Han , Si Jae Park
发明人： Sang Yup Lee , Mee-Jung Han , Si Jae Park
IPC分类号： C07K1/26
CPC分类号： C07K14/195 , C07K14/00
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional (2-D) gel electrophoresis. Furthermore, the present invention relates to the improved method of 2-D gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the 2-D gel electrophoresis, thereby obtaining 2-D gel with much more protein spots.
摘要翻译：本发明涉及含有用于预防蛋白质降解的sHSP和用于二维（2-D）凝胶电泳的组合物的组合物。此外，本发明涉及2-D凝胶电泳的改进方法，其特征在于使用sHSP。根据本发明，在2-D凝胶电泳中防止了蛋白质斑点的减少，从而获得了具有更多蛋白质斑点的2-D凝胶。

7. 发明授权

US09120891B2 Method for preparing polylactate and copolymer thereof using a mutant microorganism with enhanced polylactate, and the copolymer producing capability thereof 有权
标题翻译：使用具有增强的聚乳酸的突变微生物制备聚乳酸的方法及其共聚物及其共聚物生产能力
公开(公告)号：US09120891B2
公开(公告)日：2015-09-01
申请号：US13001001
申请日：2009-06-24
申请人： Sang Yup Lee , Yu Kyung Jung , Taek Ho Yang , Si Jae Park , Tae Wan Kim
发明人： Sang Yup Lee , Yu Kyung Jung , Taek Ho Yang , Si Jae Park , Tae Wan Kim
IPC分类号： C12P7/18 , C12P7/42 , C12N1/20 , C12N15/74 , C07H21/04 , C08G63/06 , C12P7/62 , C12P21/02
CPC分类号： C08G63/06 , C12P7/625 , C12P21/02
摘要： Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.
摘要翻译：提供了使用突变微生物制备聚乳酸（PLA）或其共聚物的方法，其中遗传操作参与辅酶A（CoA）供体和乳酸生产途径的基因以提高CoA供体和乳酸的生产率。 CoA供体和乳酸盐的量在微生物代谢途径中同时增加，以有效生物合成PLA和具有高含量乳酸盐的羟基链烷酸酯 - 乳酸共聚物，其在工业上是有用的。

8. 发明申请

US20140030775A1 RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE [LACTATE-CO-GLYCOLATE] COPOLYMER FROM GLUCOSE, AND METHOD FOR PREPARING [LACTATE-CO-GLYCOLATE] COPOLYMER USING SAME 有权
标题翻译：具有从葡萄糖生产[柠檬酸 - 甘油酯]共聚物的能力的重组微生物以及使用其制备[LACTATE-CO-GLYCOLATE]共聚物的方法
公开(公告)号：US20140030775A1
公开(公告)日：2014-01-30
申请号：US14004437
申请日：2012-03-09
申请人： Sang Yup Lee , Si Jae Park , Seung Hwan Lee , Bong Keun Song , Yu Kyung Jung , Tae Woo Lee
发明人： Sang Yup Lee , Si Jae Park , Seung Hwan Lee , Bong Keun Song , Yu Kyung Jung , Tae Woo Lee
IPC分类号： C12P7/62 , C12N15/70
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12N15/70
摘要： There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment.
摘要翻译：提供了一种具有来自葡萄糖的聚（乳酸 - 共 - 乙醇酸）的生产性的重组微生物，更具体而言，是在不添加外源乙醇酸酯前体的情况下具有聚（乳酸 - 共 - 乙醇酸）的生产性的重组微生物， [聚（制备乳酸 - 共 - 乙醇酸）]。根据本发明，可以高浓度制备其中乙醇酸酯部分浓度高的聚（乳酸 - 共 - 乙醇酸），而不提供外源乙醛酸酯。因此，本发明可以有效地用于治疗。

9. 发明申请

US20100330634A1 Mutants Having Capability To Produce 1, 4-Butanediol And Method For Preparing 1, 4-Butanediol Using The Same 有权
标题翻译：具有生产1,4-丁二醇能力的突变体及其制备1,4-丁二醇的方法
公开(公告)号：US20100330634A1
公开(公告)日：2010-12-30
申请号：US12676840
申请日：2008-08-13
申请人： Si Jae Park , Sang Hyun Lee , Sang Yup Lee , Eun Jeong Lee
发明人： Si Jae Park , Sang Hyun Lee , Sang Yup Lee , Eun Jeong Lee
IPC分类号： C12P7/18 , C12N1/19 , C12N1/15 , C12N1/21 , C07H21/04 , C12N15/63
CPC分类号： C12N15/77 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12P7/18 , C12Y101/01061 , C12Y102/01024 , C12Y103/99002
摘要： A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.
摘要翻译：提供能够生产1,4-丁二醇的突变体和使用其制备1,4-丁二醇的方法。通过在能够产生琥珀酸的微生物中引入和扩增编码将琥珀酸酯转化成4-羟基丁酸酯和4-羟基丁酸酯的基因转化成1,4-丁二醇来制备突变体微生物。该方法包括在含有碳水化合物的培养基中培养该突变体并从培养物中获得1,4-丁二醇。因此，在化学工业中必不可少的1,4-丁二醇可以在生物过程中制备。

10. 发明授权

US08524478B2 Mutant of propionyl-CoA transferase from Clostridium propionicum and preparing method for PLA or PLA copolymer using the same 有权
标题翻译：丙酸酰辅酶A转移酶由丙酸梭菌的突变体及使用其制备PLA或PLA共聚物的制备方法
公开(公告)号：US08524478B2
公开(公告)日：2013-09-03
申请号：US12673389
申请日：2008-07-25
申请人： Si Jae Park , Taek Ho Yang , Hye Ok Kang , Sang Hyun Lee , Eun Jung Lee , Tae Wan Kim
发明人： Si Jae Park , Taek Ho Yang , Hye Ok Kang , Sang Hyun Lee , Eun Jung Lee , Tae Wan Kim
IPC分类号： C12N1/10 , C12P7/62 , C07H21/04
CPC分类号： C12P7/625 , C12N9/1029 , C12N9/1048 , C12N9/13 , C12Y203/01 , C12Y208/03001 , Y02P20/52
摘要： Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.
摘要翻译：本发明提供一种丙酸酰辅酶A转移酶突变体，其可从丙酸梭菌中在使用微生物制备聚乳酸（PLA）或PLA共聚物的方法中以高效率将乳酸转化为乳酰辅酶A。不同于在大肠杆菌中弱表达的常规丙酰基-CoA转移酶，当将来自丙酸梭菌的丙酰辅酶A转移酶的突变体引入重组大肠杆菌中时，可以非常顺利地提供乳酰辅酶A，从而能够高效制备聚乳酸（PLA）和PLA共聚物。

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